812 research outputs found
Digital Preservation and Access of Natural Resources Documents
Digitization and preservation of natural resource documents were reviewed and the current status of digitization presented for a North American university. It is important to present the status of the digitation process for natural resources and to advocate for increased collections of digital material for ease of reference and exchange of information. Digital collections need to include both published documents and ancillary material for research projects and data for future use and interpretation. The methods in this paper can be applied to other natural resource collections increasing their use and distribution. The process of decision making for documents and their preservation and inclusion in ScholarWorks is presented as a part of the Forest Sciences Commons as a subset of the Life Sciences Commons of the Digital Commons Open Network launched and maintained by bepress. Digitization has increased the roles and skillsets needed for librarians and from libraries. This creates new challenges and opportunities for the library as publisher and as an advocate for open access. Digital curation melds together digitization and knowledge management and enhances community engagement. Digitization of collections are reviewed and natural resource documentation presented for faculty publications, Research Projects and Centers, eBooks, Journals, Galleries and electronic Theses and Dissertations (ETDs). Recommendations are made to increase the digital curation of the collection by encouraging community participation and use. Digital archives are important to natural resource professionals as society-ready natural resource graduates need to deal effectively with complex ecological, economic and social issues of current natural resources management. Natural resource research for the future needs to ensure that professionals have a greater breath of knowledge as they interpret and apply new knowledge, understanding, and technology to complex, transdisciplinary social and biological issues and challenges
Accretion onto the Supermassive Black Hole in M87
Chandra X-ray observations of the giant elliptical galaxy M87 resolve the
thermal state of the hot interstellar medium into the accretion (Bondi) radius
of its central 3 10^9 Msun black hole. We measure the X-ray gas temperature and
density profiles and calculate the Bondi accretion rate, Mdot_Bondi \sim 0.1
Msun/yr. The X-ray luminosity of the active nucleus of M87 observed with
Chandra is L_{x, 0.5-7 \keV} \sim 7 \times 10^{40}erg/s. This value is much
less than the predicted nuclear luminosity, L_{Bondi} \sim 5 \times 10^{44}
erg/s, for accretion at the Bondi rate with a canonical accretion radiative
efficiency of 10%. If the black hole in M87 accretes at this rate it must do so
at a much lower radiative efficiency than the canonical value. The
multiwavelength spectrum of the nucleus is consistent with that predicted by an
advection-dominated flow. However, as is likely, the X-ray nucleus is dominated
by jet emission then the properties of flow must be modified, possibly by
outflows. We show that the overall energetics of the system are just consistent
with the predicted Bondi nuclear power. This suggests that either most of the
accretion energy is released in the relativistic jet or that the central engine
of M87 undergoes on-off activity cycles. We show that, at present, the energy
dumped into the ISM by the jet may reduce the accretion rate onto the black
hole by a factor \propto (v_j/c_s)^{-2}, where v_j is the jet velocity and c_s
the ISM sound speed, and that this is sufficient to account for the low nuclear
luminosity.Comment: emulateapj.sty, revised version, accepted by Ap
A high-resolution wide-field radio survey of M51
We present the highest resolution, wide-field radio survey of a nearby face-on star-forming galaxy to date. The multiphase centre technique is used to survey the entire disc of M51 (77 arcmin2) at a maximum resolution of 5 mas on a single 8 h pointing with the European Very Long Baseline Interferometry (VLBI) Network at 18 cm. In total, 7 billion pixels were imaged using 192 phase centres that resulted in the detection of six sources: the Seyfert nucleus, the supernova SN 2011dh, and four background AGNs. Using the wealth of archival data available in the radio (Multi-Element Radio Linked Interferometer Network and the Very Large Array), optical (Hubble Space Telescope) and X-rays (Chandra) the properties of the individual sources were investigated in detail. The combined multiwavelength observations reveal a very complex and puzzling core region that includes a low-luminosity parsec scale core-jet structure typical of AGNs, with evidence for a lateral shift corresponding to 0.27c. Furthermore, there is evidence for a fossil radio hotspot located 1.44 kpc from the Seyfert nucleus that may have resulted from a previous ejection cycle. Our study provides measures of the supernova and star formation rates that are comparable to independent studies at other wavelengths, and places further limits on the radio and X-ray luminosity evolution of the supernovae SN 1994I, SN 2005cs and SN 2011dh. The radio images of background AGN reveal complex morphologies that are indicative of powerful radio galaxies, and confirmed via the X-ray and optical properties
Resolved Spectroscopy of the Narrow-Line Region in NGC 1068. II. Physical Conditions Near the NGC 1068 ``Hot-Spot''
The physical conditions near the optical continuum peak (``hot spot'') in the
inner narrow line region (NLR) of the Seyfert 2 galaxy, NGC 1068. Spectra were
taken with HST/STIS through the 0.1X52 arcsec slit, covering the full STIS 1200
to 10000 Angstrom waveband, and are from a region that includes the hot spot,
extending 0.2, or ~ 14 pc (for H= 75 km/sec/Mpc). Perhaps the most striking
feature of these spectra is the presence of strong coronal emission lines,
including [S XII] 7611 which has hitherto only been identified in spectra of
the solar corona. There is an apparent correlation between ionization energy
and velocity of the emission lines with respect to the systemic velocity of the
host galaxy, with the coronal lines blueshifted, most other high excitation
lines near systemic, and some of the low ionization lines redshifted. From the
results of our modeling, we find that the emission-line gas consists of three
principal components: 1) one in which most of the strong emission-lines, such
as [O III] 5007, [Ne V] 3426, C IV 1550, arise, 2) a more tenuous, highly
ionized component, which is the source of the coronal-line emission, and 3) a
component, which is not co-planar with the other two, in which the low
ionization and neutral lines, such as [N II] 6548 and [O I] 6300, are formed.
The first two components are directly ionized by the EUV-Xray continuum emitted
by the central source, while the low ionization gas is ionized by a combination
of highly absorbed continuum radiation and a small fraction of unabsorbed
continuum scattered by free electrons associated with the hot spot. The
combination of covering factor and Thomson optical depth of the high ionization
components is insufficient to scatter the observed fraction of continuum
radiation into our line-of-sight.Comment: 42 pages, Latex, includes 5 figures (postscript), to appear in the
Astrophysical Journa
TRAIL and proteasome inhibitors combination induces a robust apoptosis in human malignant pleural mesothelioma cells through Mcl-1 and Akt protein cleavages
Background
Malignant pleural mesothelioma (MPM) is an aggressive malignancy closely associated with asbestos exposure and extremely resistant to current treatments. It exhibits a steady increase in incidence, thus necessitating an urgent development of effective new treatments. Methods
Proteasome inhibitors (PIs) and TNFα-Related Apoptosis Inducing Ligand (TRAIL), have emerged as promising new anti-MPM agents. To develop effective new treatments, the proapoptotic effects of PIs, MG132 or Bortezomib, and TRAIL were investigated in MPM cell lines NCI-H2052, NCI-H2452 and NCI-H28, which represent three major histological types of human MPM. Results
Treatment with 0.5-1 ÎŒM MG132 alone or 30 ng/mL Bortezomib alone induced a limited apoptosis in MPM cells associated with the elevated Mcl-1 protein level and hyperactive PI3K/Akt signaling. However, whereas 10â20 ng/ml TRAIL alone induced a limited apoptosis as well, TRAIL and PI combination triggered a robust apoptosis in all three MPM cell lines. The robust proapoptotic activity was found to be the consequence of a positive feedback mechanism-governed amplification of caspase activation and cleavage of both Mcl-1 and Akt proteins, and exhibited a relative selectivity in MPM cells than in non-tumorigenic Met-5A mesothelial cells. Conclusion
The combinatorial treatment using TRAIL and PI may represent an effective new treatment for MPMs
Intrinsic Absorption Lines in Seyfert 1 Galaxies. I. Ultraviolet Spectra from the Hubble Space Telescope
We present a study of the intrinsic absorption lines in the ultraviolet
spectra of Seyfert 1 galaxies. We find that the fraction of Seyfert 1 galaxies
that show absorption associated with their active nuclei is more than one-half
(10/17), which is much higher than previous estimates (3 - 10%) . There is a
one-to-one correspondence between Seyferts that show intrinsic UV absorption
and X-ray ``warm absorbers''. The intrinsic UV absorption is generally
characterized by high ionization: C IV and N V are seen in all 10 Seyferts with
detected absorption (in addition to Ly-alpha), whereas Si IV is present in only
four of these Seyferts, and Mg II absorption is only detected in NGC 4151. The
absorption lines are blueshifted (or in a few cases at rest) with respect to
the narrow emission lines, indicating that the absorbing gas is undergoing net
radial outflow. At high resolution, the absorption often splits into distinct
kinematic components that show a wide range in widths (20 - 400 km/s FWHM),
indicating macroscopic motions (e.g., radial velocity subcomponents or
turbulence) within a component. The strong absorption components have cores
that are much deeper than the continuum flux levels, indicating that the
regions responsible for these components lie completely outside of the broad
emission-line regions. The covering factor of the absorbing gas in the line of
sight, relative to the total underlying emission, is C > 0.86, on average. The
global covering factor, which is the fraction of emission intercepted by the
absorber averaged over all lines of sight, is C > 0.5.Comment: 56 pages, Latex, includes 4 figures (encapsulated postscript), Fig. 1
has 2 parts and Fig. 2 has 3 parts, to appear in the Astrophysical Journa
Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells.
Background:
The unique physicochemical properties of multi-walled carbon nanotubes (MWCNT) have led to many industrial applications. Due to their low density and small size, MWCNT are easily aerosolized in the workplace making respiratory exposures likely in workers. The International Agency for Research on Cancer designated the pristine Mitsui-7 MWCNT (MWCNT-7) as a Group 2B carcinogen, but there was insufficient data to classify all other MWCNT. Previously, MWCNT exposed to high temperature (MWCNT-HT) or synthesized with nitrogen (MWCNT-ND) have been found to elicit attenuated toxicity; however, their genotoxic and carcinogenic potential are not known. Our aim was to measure the genotoxicity of MWCNT-7 compared to these two physicochemically-altered MWCNTs in human lung epithelial cells (BEAS-2B & SAEC).
Results:
Dose-dependent partitioning of individual nanotubes in the cell nuclei was observed for each MWCNT material and was greatest for MWCNT-7. Exposure to each MWCNT led to significantly increased mitotic aberrations with multi- and monopolar spindle morphologies and fragmented centrosomes. Quantitative analysis of the spindle pole demonstrated significantly increased centrosome fragmentation from 0.024-2.4 [mu]g/mL of each MWCNT. Significant aneuploidy was measured in a dose-response from each MWCNT-7, HT, and ND; the highest dose of 24 [mu]g/mL produced 67, 61, and 55%, respectively. Chromosome analysis demonstrated significantly increased centromere fragmentation and translocations from each MWCNT at each dose. Following 24 h of exposure to MWCNT-7, ND and/or HT in BEAS-2B a significant arrest in the G1/S phase in the cell cycle occurred, whereas the MWCNT-ND also induced a G2 arrest. Primary SAEC exposed for 24 h to each MWCNT elicited a significantly greater arrest in the G1 and G2 phases. However, SAEC arrested in the G1/S phase after 72 h of exposure. Lastly, a significant increase in clonal growth was observed one month after exposure to 0.024 [mu]g/mL MWCNT-HT & ND.
Conclusions:
Although MWCNT-HT & ND cause a lower incidence of genotoxicity, all three MWCNTs cause the same type of mitotic and chromosomal disruptions. Chromosomal fragmentation and translocations have not been observed with other nanomaterials. Because in vitro genotoxicity is correlated with in vivo genotoxic response, these studies in primary human lung cells may predict the genotoxic potency in exposed human populations
Phenotypic Instability and Rapid Gene Silencing in Newly Formed Arabidopsis Allotetraploids
Discovery of induced point mutations in maize genes by TILLING
BACKGROUND: Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern genomics era; however, the most powerful methods are generally restricted to a few model organisms. Previously, we introduced a reverse-genetic strategy with the potential for general applicability to organisms that lack well-developed genetic tools. Our TILLING (Targeting Induced Local Lesions IN Genomes) method uses chemical mutagenesis followed by screening for single-base changes to discover induced mutations that alter protein function. TILLING was shown to be an effective reverse genetic strategy by the establishment of a high-throughput TILLING facility and the delivery of thousands of point mutations in hundreds of Arabidopsis genes to members of the plant biology community. RESULTS: We demonstrate that high-throughput TILLING is applicable to maize, an important crop plant with a large genome but with limited reverse-genetic resources currently available. We screened pools of DNA samples for mutations in 1-kb segments from 11 different genes, obtaining 17 independent induced mutations from a population of 750 pollen-mutagenized maize plants. One of the genes targeted was the DMT102 chromomethylase gene, for which we obtained an allelic series of three missense mutations that are predicted to be strongly deleterious. CONCLUSIONS: Our findings indicate that TILLING is a broadly applicable and efficient reverse-genetic strategy. We are establishing a public TILLING service for maize modeled on the existing Arabidopsis TILLING Project
Identifying Gamma-Ray Burst Remnants Through Positron Annihilation Radiation
We model the annihilation of relic positrons produced in a gamma-ray burst
(GRB) after its afterglow has faded. We find that the annihilation signal from
at least one GRB remnant in the Milky Way galaxy should be observable with
future space missions such as INTEGRAL and EXIST, provided that the gas
surrounding the GRB source has the typical density of the interstellar medium,
< 1 cm^-3. Three fortunate circumstances conspire to make the signal
observable. First, unlike positrons in a standard supernova, the GRB positrons
initially travel at a relativistic speed and remain ahead of any
non-relativistic ejecta until the ejecta become rarefied and the annihilation
time becomes long. Second, the GRB remnant remains sufficiently hot (T > 5 x
10^5 K) for a strong annihilation line to form without significant smearing by
three-photon decay of positronium. Third, the annihilation signal persists over
a time longer than the average period between GRB events in the Milky Way
galaxy.Comment: 5 pages, 2 figures, submitted to ApJL (fixed Latex figure
referencing
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