Atypical enteropathogenic Escherichia coli (aEPEC) in children under five years old with diarrhea in Quito (Ecuador)

Enteropathogenic Escherichia coli (EPEC) remain one the most important pathogens infecting children and they are one of the main causes of persistent diarrhea worldwide. In this study, we have isolated EPEC from 94 stool samples of children under five years old with diarrheal illness in the area of Quito (Ecuador), and we have determined the occurrence of the two subtypes of EPEC, typical EPEC (tEPEC) and atypical (aEPEC), by PCR amplification of the genes eae (attaching and effacing) and bfp (bundle- forming pilus). Typical EPEC is positive for eae and bfp genes while aEPEC is positive only for eae. Our results suggest that aEPEC is the most prevalent subtype in Quito (89.36 %), while subtype tEPEC is less prevalent (10.64 %). [Int Microbiol 19(3):157-160 (2016)]Keywords: Escherichia coli · atypical EPEC · genes eae and bfp · diarrhea in children · Quito (Ecuador

SYMPA, a dedicated instrument for Jovian Seismology. II. Real performance and first results

Context. Due to its great mass and its rapid formation, Jupiter has played a crucial role in shaping the Solar System. The knowledge of its internal structure would strongly constrain the solar system formation mechanism. Seismology is the most efficient way to probe directly the internal structure of giant planets. Aims. SYMPA is the first instrument dedicated to the observations of free oscillations of Jupiter. Principles and theoretical performance have been presented in paper I. This second paper describes the data processing method, the real instrumental performance and presents the first results of a Jovian observation run, lead in 2005 at Teide Observatory. Methods. SYMPA is a Fourier transform spectrometer which works at fixed optical path difference. It produces Doppler shift maps of the observed object. Velocity amplitude of Jupiter's oscillations is expected below 60 cm/s. Results Despite light technical defects, the instrument demonstrated to work correctly, being limited only by photon noise, after a careful analysis. A noise level of about 12 cm/s has been reached on a 10-night observation run, with 21 % duty cycle, which is 5 time better than previous similar observations. However, no signal from Jupiter is clearly highlighted.Comment: 13 pages, 26 figure

77^{77}Se and 63^{63}Cu NMR studies of the electronic correlations in Cux_xTiSe2_2 (x=0.05,0.07x=0.05, 0.07)

We report $^{77}$Se and $^{63}$Cu nuclear magnetic resonance (NMR) investigation on the charge-density-wave (CDW) superconductor Cu$_x$TiSe$_2$ ($x=0.05$ and 0.07). At high magnetic fields where superconductivity is suppressed, the temperature dependence of $^{77}$Se and $^{63}$Cu spin-lattice relaxation rates 1/T_{1}$follow a linear relation. The slope of$^{77}1/T_{1}$ vs \emph{T} increases with the Cu doping. This can be described by a modified Korringa relation which suggests the significance of electronic correlations and the Se 4\emph{p}- and Ti 3\emph{d}-band contribution to the density of states at the Fermi level in the studied compounds.Comment: Revised manuscript. Submitted to Journal of Physics: Condensed Matte

Inter-annual ciliate distribution variation within the late stratification oxycline in a monomictic lake, Lake Alchichica (Mexico)

Deep stratified tropical water bodies pass through prolonged periods of meta-hypolimnetic anoxia, and ciliates might play a very important role in the plankton community budget there. We analysed changes in the composition and biomass of the ciliate assemblage and other microbial loop components throughout the oxycline just at the end of stratification in a warm-monomictic lake, Lake Alchichica, Mexico (four samplings: 2006-2008, 2010); the results were compared with those obtained from another lake from the re- gion, La Preciosa, sampled in 2010. Bacteria, autotrophic picoplankton (APP) and flagellates were analysed using epifluorescence microscopy. Ciliates were evaluated either in DAPI stained samples (looking for pigmented organelles and/or ingested phototrophs) or in quantitative protargol stain (QPS) permanent preparations, where they were identified at the genus or species level. The end of the stratification period in Lake Alchichica was characterized by almost uniform heterotrophic picoplankton (HPP) numbers (106 cells mL–1 ) throughout the water column. Meanwhile, APP showed epilimnetic and/or metalimnetic maxima of 105 cells mL–1 followed by an order of magnitude drop in the hypolimnion. A very important peak (105 cells mL–1 ) of the autotrophic or mixotrophic flagellate Pyramimonas sp. was observed repeatedly above and within the oxycline of Lake Alchichica. Ciliate biomass maxima were found around the oxycline and in the above-bottom layer. The top of the oxycline was dominated by Euplotes spp. and Spirostomum teres fine- to coarse-filter feeders (feeding upon APP, nanodiatoms and algae). Raptorial haptorids (in particular, Phialina sp.) were the second most important group, generally occupying the layer below euplotids, followed by Holophrya and Prorodon facultative anaer- obic prostomes. Sometimes, strictly anaerobic Caenomorpha sp. was found to be important in the anoxic hypolimnion. Minute pi- coplankton feeding species (both APP and heterotrophic bacteria feeders) were important throughout the water column: in the epilimnion, vorticellids (2006-2008) or scuticociliates (2010) dominated. Typically, the scuticociliate maximum was located in the oxycline and/or above the bottom. Some microaerophilic species were isolated; thus, their identification could be carried out. However, the apparent polymorphic ciliate life cycles were not described completely, and the species composition was only estimated: two dom- inant species (SC 1 - Cristigera-like and SC 2 - Cyclidim-like) covered nearly the total scuticociliate biomass. Strictly anaerobic scu- ticociliates were not isolated but observed in the deepest layers of the lake (bacteria symbiotic Isocyclidium globosum and Cristigera sp.). Significant statistical relation within the ciliate distribution and environmental variables was not confirmed due to unique species composition in the respective years. However, general trends in the distribution of ciliates on a species level were observed. Scutic- ociliates, including two important tentatively identified species, did not present unambiguous ecological position, and the study of their live cycle should be the next step in investigations

Onset of Phase Synchronization in Neurons Conneted via Chemical Synapses

We study the onset of synchronous states in realistic chaotic neurons coupled by mutually inhibitory chemical synapses. For the realistic parameters, namely the synaptic strength and the intrinsic current, this synapse introduces non-coherences in the neuronal dynamics, yet allowing for chaotic phase synchronization in a large range of parameters. As we increase the synaptic strength, the neurons undergo to a periodic state, and no chaotic complete synchronization is found.Comment: to appear in Int. J. Bif. Chao

Chiral bosons and improper constraints

We argue that a consistent quantization of the Floreanini-Jackiw model, as a constrained system, should start by recognizing the improper nature of the constraints. Then each boundary conditon defines a problem which must be treated sparately. The model is settled on a compact domain which allows for a discrete formulation of the dynamics; thus, avoiding the mixing of local with collective coordinates. For periodic boundary conditions the model turns out to be a gauge theory whose gauge invariant sector contains only chiral excitations. For antiperiodoc boundary conditions, the mode is a second-class theory where the excitations are also chiral. In both cases, the equal-time algebra of the quantum energy-momentum densities is a Virasoro algebra. The Poincar\'e symmetry holds for the finite as well as for the infinite domain.Comment: 13 pages, Revtex file, IF.UFRGS Preprin

Approximating Mexican highways with slime mould

Plasmodium of Physarum polycephalum is a single cell visible by unaided eye. During its foraging behavior the cell spans spatially distributed sources of nutrients with a protoplasmic network. Geometrical structure of the protoplasmic networks allows the plasmodium to optimize transport of nutrients between remote parts of its body. Assuming major Mexican cities are sources of nutrients how much structure of Physarum protoplasmic network correspond to structure of Mexican Federal highway network? To find an answer undertook a series of laboratory experiments with living Physarum polycephalum. We represent geographical locations of major cities by oat flakes, place a piece of plasmodium in Mexico city area, record the plasmodium's foraging behavior and extract topology of nutrient transport networks. Results of our experiments show that the protoplasmic network formed by Physarum is isomorphic, subject to limitations imposed, to a network of principle highways. Ideas and results of the paper may contribute towards future developments in bio-inspired road planning