241 research outputs found
Stimuli-responsive behavior of PNiPAm microgels under interfacial confinement
The volume phase transition of microgels is one of the most paradigmatic
examples of stimuli-responsiveness, enabling a collapse from a highly swollen
microgel state into a densely coiled state by an external stimulus. Although
well characterized in bulk, it remains unclear how the phase transition is
affected by the presence of a confining interface. Here, we demonstrate that
the temperature-induced volume phase transition of poly(N-isopropylacrylamide)
microgels, conventionally considered an intrinsic molecular property of the
polymer, is in fact largely suppressed when the microgel is adsorbed to an
air/liquid interface. We further observe a hysteresis in core morphology and
interfacial pressure between heating and cooling cycles. Our results, supported
by molecular dynamics simulations, reveal that the dangling polymer chains of
microgel particles, spread at the interface under the influence of surface
tension, do not undergo any volume phase transition, demonstrating that the
balance in free energy responsible for the volume phase transition is
fundamentally altered by interfacial confinement. These results imply that
important technological properties of such systems, including the
temperature-induced destabilization of emulsions does not occur via a decrease
in interfacial coverage of the microgels
Author Correction: Drosophila ĂHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.
In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ĂHeavy-Spectrin cytoskeleton. ĂHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function
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Author Correction: Drosophila ĂHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.
Soft particles at liquid interfaces: From molecular particle architecture to collective phase behavior
Soft particles such as microgels and core-shell particles can undergo
significant and anisotropic deformations when adsorbed to a liquid interface.
This, in turn, leads to a complex phase behavior upon compression. Here we
develop a multiscale framework to rationally link the molecular particle
architecture to the resulting interfacial morphology and, ultimately, to the
collective interfacial phase behavior, enabling us to identify the key
single-particle properties underlying two-dimensional continuous,
heterostructural, and isostructural solid-solid transitions. Our approach
resolves existing discrepancies between experiments and simulations and thus
provides a unifying framework to describe phase transitions in interfacial
soft-particle systems. We establish proof-of-principle for our rational
approach by synthesizing three different poly(N-isopropylacrylamide)
soft-particle architectures, each of which corresponds to a different targeted
phase behavior. In parallel, we introduce a versatile and highly efficient
coarse-grained simulation method that adequately captures the qualitative key
features of each soft-particle system; the novel ingredient in our simulation
model is the use of auxiliary degrees of freedom to explicitly account for the
swelling and collapse of the particles as a function of surface pressure.
Notably, these combined efforts allow us to establish the first experimental
demonstration of a heterostructural transition to a chain phase in a
single-component system, as well as the first accurate in silico account of the
two-dimensional isostructural transition. Overall, our multiscale framework
provides a bridge between physicochemical soft-particle characteristics at the
molecular- and nanoscale and the collective self-assembly phenomenology at the
macroscale, paving the way towards novel materials with on-demand interfacial
behavior
Interface-induced hysteretic volume phase transition of microgels: simulation and experiment
Thermo-responsive microgel particles can exhibit a drastic volume shrinkage
upon increasing the solvent temperature. Recently we found that the spreading
of poly(N-isopropylacrylamide)(PNiPAm) microgels at a liquid interface under
the influence of surface tension hinders the temperature-induced volume phase
transition. In addition, we observed a hysteresis behavior upon temperature
cycling, i.e. a different evolution in microgel size and shape depending on
whether the microgel was initially adsorbed to the interface in expanded or
collapsed state. Here, we model the volume phase transition of such microgels
at an air/water interface by monomer-resolved Brownian dynamics simulations and
compare the observed behavior with experiments. We reproduce the experimentally
observed hysteresis in the microgel dimensions upon temperature variation. Our
simulations did not observe any hysteresis for microgels dispersed in the bulk
liquid, suggesting that it results from the distinct interfacial morphology of
the microgel adsorbed at the liquid interface. An initially collapsed microgel
brought to the interface and subjected to subsequent swelling and collapsing
(resp. cooling and heating) will end up in a larger size than it had in the
original collapsed state. Further temperature cycling, however, only shows a
much reduced hysteresis, in agreement with our experimental observations. We
attribute the hysteretic behavior to a kinetically trapped initial collapsed
configuration, which relaxes upon expanding in the swollen state. We find a
similar behavior for linear PNiPAm chains adsorbed to an interface. Our
combined experimental - simulation investigation provides new insights into the
volume phase transition of PNiPAm materials adsorbed to liquid interfaces
Drosophila Ă Heavy -Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil
Abstract: In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ĂHeavy-Spectrin cytoskeleton. ĂHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function
Drosophila Ă Heavy -Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil
Abstract: In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ĂHeavy-Spectrin cytoskeleton. ĂHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function
The spectral action and cosmic topology
The spectral action functional, considered as a model of gravity coupled to
matter, provides, in its non-perturbative form, a slow-roll potential for
inflation, whose form and corresponding slow-roll parameters can be sensitive
to the underlying cosmic topology. We explicitly compute the non-perturbative
spectral action for some of the main candidates for cosmic topologies, namely
the quaternionic space, the Poincare' dodecahedral space, and the flat tori. We
compute the corresponding slow-roll parameters and see we check that the
resulting inflation model behaves in the same way as for a simply-connected
spherical topology in the case of the quaternionic space and the Poincare'
homology sphere, while it behaves differently in the case of the flat tori. We
add an appendix with a discussion of the case of lens spaces.Comment: 55 pages, LaTe
First evidence of population genetic structure of the deep-water blackmouth catshark Galeus melastomus Rafinesque, 1810
Genetic connectivity at large spatial scales. Given the lack of species-specific nuclear markers, a total of 129 microsatellite loci (Simple Sequence Repeats, SSRs) were cross-amplified on blackmouth catshark specimens collected in eight geographically distant areas in the Mediterranean Sea and North-eastern Atlantic Ocean. A total of 13 SSRs were finally selected for genotyping, based on which the species exhibited signs of weak, but tangible genetic structure. The clearcut evidence of genetic differentiation of G. melastomus from Scottish waters from the rest of the population samples was defined, indicating that the species is genetically structured in the Mediterranean Sea and adjacent Southern North-eastern Atlantic. Both individual and frequency-based analyses identified a genetic unit formed by the individuals collected in the Tyrrhenian Sea and the Strait of Sicily, distinguished from the rest of the Mediterranean and Portuguese samples. In addition, Bayesian analyses resolved a certain degree of separation of the easternmost Aegean sample and the admixed nature of the other Mediterranean and the Portuguese samples. Here, our results supported the hypothesis that the interaction between the ecology and biology of the species and abiotic drivers such as water circulations, temperature and bathymetry may affect the dispersion of G. melastomus, adding new information to the current knowledge of the connectivity of this deep-water species and providing powerful tools for estimating its response to anthropogenic impacts
Extending Timescales and Narrowing Linewidths in NMR
Among the different fields of research in nuclear magnetic resonance (NMR) which are currently investigated in the Laboratory of Biomolecular Magnetic Resonance (LRMB), two subjects that are closely related to each other are presented in this article. On the one hand, we show how to populate long-lived states (LLS) that have long lifetimes T_LLS which allow one to go beyond the usual limits imposed by the longitudinal relaxation time T_1. This makes it possible to extend NMR experiments to longer time-scales. As an application, we demonstrate the extension of the timescale of diffusion measurements by NMR spectroscopy. On the other hand, we review our work on long-lived coherences (LLC), a particular type of coherence between two spin states that oscillates with the frequency of the scalar coupling constant J_IS and decays with a time constant T_LLC. Again, this time constant T_LLC can be much longer than the transverse relaxation time T_2. By extending the coherence lifetimes, we can narrow the linewidths to an unprecedented extent. J-couplings and residual dipolar couplings (RDCs) in weakly-oriented phases can be measured with the highest precision
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