Automated clinical system for chromosome analysis

An automatic chromosome analysis system is provided wherein a suitably prepared slide with chromosome spreads thereon is placed on the stage of an automated microscope. The automated microscope stage is computer operated to move the slide to enable detection of chromosome spreads on the slide. The X and Y location of each chromosome spread that is detected is stored. The computer measures the chromosomes in a spread, classifies them by group or by type and also prepares a digital karyotype image. The computer system can also prepare a patient report summarizing the result of the analysis and listing suspected abnormalities

Physical activity patterns in a nationally representative sample of adults in Ireland

Original article can be found at: http://journals.cambridge.org/ Copyright - the authors. DOI: 10.1079/PHN2001192Objective To evaluate habitual levels of physical activity in a nationally representative sample of adults in Ireland. Design Cross-sectional survey using a self-administered questionnaire. Usual levels of work, recreational and household activities were evaluated in relation to anthropometric, demographic and socio-economic characteristics. The amount and intensity of all activities were quantified by assigning metabolic equivalents (METS) to each activity. Setting Republic of Ireland and Northern Ireland, 1997–1999. Subjects Random sample of 1379 adults aged 18–64 years. Results Men were approximately twice as active in work and recreational activity (139.7 ± 83.9 METS) as women (68.5 ± 49.8 METS; P 28kg m−2) or obese (BMI > 30kg m−2). Fewer obese subjects reported higher levels of work and leisure activities. However, a higher percentage of obese women reported participation in the higher levels of household activities. Participation rates in recreational activities were low. Walking was the most important leisure activity of both men (41%) and women (60%). In terms of hours per week spent in vigorous physical activity, men were more active than women, professional and skilled non-manual women were more active than women in other social classes, and younger subjects (aged 18–35 years) were more active than older subjects. Conclusions The holistic approach used in the assessment of physical activity in this study has revealed important and subtle differences in the activity patterns of men and women. Failure to fully characterise the respective activity patterns of men and women could lead to ill-informed public health policy aimed at promoting and sustaining lifetime habits of physical activity. The results suggest that simple population-focused programmes to promote physical activity are unlikely to offer the same chance of long-term success as more sensitive and individualised strategies.Peer reviewe

Ionic Liquids Containing Sulfonium Cations as Electrolytes for Electrochemical Double Layer Capacitors

In this work, we report on the behavior of ionic liquids (ILs) containing sulfonium cations as electrolytes for electrochemical double layer capacitors (EDLCs). Physical properties such as viscosity and ionic conductivity are reported over a range of temperatures for ILs containing the diethylmethyl sulfonium [S221], triethyl sulfonium [S222], and diethylpropyl sulfonium [S223] cations paired with the bis(trifluoromethanesulfonyl) imide [Tf2N] anion. The size and structure of the cations are shown to influence the physical and electrochemical properties of the ILs, with a significant degree of ionic coordination being evident in [S223][Tf2N]. The electrochemical behavior of these ILs in EDLCs was compared with that of a fairly established IL electrolyte, N-butyl-N-methylpyrrolidinium bis(trifluoromethanesulfonyl) imide ([Pyr14][Tf2N]), and it is shown that [S221][Tf2N] can perform better in terms of energy and power at room temperature, despite operating at a much reduced potential

Haemolytic activity of Escherichia coli

Production of large amounts of high titre E. coli haemolysin has been achieved in a chemically-defined medium and in a glucose-nutrient broth medium. In chemically-defined medium, only cell-associated, (3-haemolysin was produced; in nutrient broth both extracellular a-haemolysin and p-haemolysin were found and, maximum yields of a-haemolysin were obtained within 2 hr after inoculation of cultures. More a-haemolysin was produced when large initial inocula were used but, loss of haemolytic activity occurred rapidly after maximum levels were reached. Evidence is presented which suggests that a-haemolysin is a "released" form of p-haemolysin. Large molecular weight proteins, contained in nutrient broth, enhanced levels of a-haemolysin without affecting growth; both forms of haemolysin required calcium ions for activity, were inhibited by incubation with trypsin and were not affected by thiomersalate (in the case of p-haemolysin inhibition of haemolysis by thiomersalate was not observed after haemolytic E, coli cells had adsorbed to sheep erythrocytes). Highly purified a-haeraolysin was obtained by precipitation methods using 50% (w/v) ammoniun sulphate (stage l), and dialysis against 0.005M acetate buffer, pH 4.6 (stage II), and by gel filtration on Sephadex G-200 at pH 7.5 an eluant buffer containing 0.01M Tris, 0.1M NaCl and 5% (w/v) glycerol (stage III). No loss of haemolytic activity was observed following dialysis at any stage in the purification. Other purification procedures, such as electrofocusing, proved of little practical value. Using the above procedures, J6% of the total activity contained in crude culture filtrates was recovered and a 4000-fold increase in specific activity was achieved. This degree of purification has not previously been reported for E. coli haemolysin. D-urine precipitation procedures, activation of haemolytic activity occurred. Furthermore, in eluant buffer containing: glycerol, the haemolysin was eluted from Sephadex G-200 near the void volume as two closely associated, but distinct peaks of activity. Several techniques were employed to estimate the molecular weight of a-haemolysin. Diffusion coefficient analysis (D20 = 2.4 x 10

A novel technique to repair orbital roof defects: irradiated homologous cadaveric rib (Tutoplast ®) graft in a recurrent frontal sinus ossifying fibroma

Ossifying fibroma in the fronto-ethmoidal sinuses is a rare, benign condition. In symptomatic cases, surgical excision is often undertaken and bony defects may be repaired using alloplastic grafts. We present a novel method of repairing an orbital roof defect using irradiated homologous cadaveric rib (Tutoplast ®) graft, overlaid with a pericranial flap. The patient made an excellent recovery, concluding that it is a viable and safe option with lower morbidity

Distribution of hepatitis B virus infection in Namibia

Background. Namibia regards hepatitis B virus (HBV) infection as a public health problem and introduced hepatitis B vaccinations for infants during 2009. However, information on HBV infection in the country remains limited, and effective public health interventions may be compromised in the absence of adequate evidence-based data. Available data from the World Health Organization (WHO) estimate that 15 - 60% of the normal population in many African countries may be positive for one or more of the HBV serological markers. Objective. To investigate the distribution of HBV infection in Namibia, using available laboratory data for 2013. Methods. A cross-sectional descriptive study was conducted using pre-existing electronic laboratory data on HBV infection. The data were retrieved from the central Namibia Institute of Pathology laboratory in Windhoek during January - December 2013. Tests were done on the following three main groups: (i) pregnant women during routine antenatal care (ANC) visits; (ii) patients with HIV/AIDS during antiretroviral therapy clinic visits; and (iii) any other individual suspected of having HBV infection. Results. Of a total of 77 238 hepatitis B surface antigen test results retrieved countrywide, 9 087 (11.8%) were positive. Of the positive results, 246/9 087 (2.7%) were in children aged 0 - 14 years, with the sexes equally affected. HBV infections increased markedly, particularly among females, in the age group 15 - 39 years, reaching a peak in the age group 30 - 34 years. Routine screening of pregnant women for HBV during ANC visits was found to be systematically conducted in only two regions, Ohangwena and Khomas. Conclusions. This study showed high proportions of positive results in pregnant women, patients with HIV/AIDS and individuals suspected of having HBV infection. The Ministry of Health and Social Services and stakeholders may wish to consider improving the routine and surveillance reporting systems for viral hepatitis and uptake of screening for pregnant women in all regions, and expanding HBV screening to other population groups. Population-based or similar studies are therefore required to determine the HBV prevalence and risk factors. This will assist Namibia in developing appropriate national viral hepatitis strategies as per WHO recommendations.S Afr Med J 2017;107(10):882-88

The rights of the child: are we creating a world in which all children are enabled to reach their full potential?

This chapter, through the lens of the UN Convention on the Rights of the Child, reviews whether we are actually creating world in which children’s development needs are recognized and met. The principle focus of the chapter is each child’s right to an education. In particular Articles 28 and 29 of the UNCRC are highlighted to provide a benchmark against which to consider education provision in multiple international contexts. Reference is made to the UN Millennium Declaration, the eight Millennium Development Goals and the UNICEF report “The State of the World’s Children 2016: A fair chance for every child” to enable examination of how a world fit for children is being achieved or not. From examination of the aforementioned reports, three imperatives emerge: economic, education and moral. These imperatives challenge the reader to consider how legislation and policy works towards or hinders the goal of creating a world in which all children are enabled to reach their full potential

Arterial Recanalization for Access for Arterial Intervention in Children: Techniques and Outcomes

PURPOSE: To assess technical success of arterial recanalization in children requiring repeated arterial access and intervention. MATERIALS AND METHODS: Over 14 years, 41 attempts to cross 30 arterial occlusions were made in 22 patients (13 male, 9 female). Median patient age was 12 months (15 days-14 years), and weight was 7.6 kg (3.0-77.3 kg). Techniques and outcomes were recorded. RESULTS: Twenty-five of 41 (61%) attempts at crossing an arterial occlusion were successful. Nineteen of 30 (63%) first attempts to cross occlusions were successful, and 6 of 11 (55%) repeat attempts were successful. The occluded segments were combinations of common femoral artery (n = 4), external iliac artery (n = 36), common iliac artery (n = 11), and aorta (n = 1). Complications occurred in 5 of 41(12%) attempts: 3 minor complications (hematoma, extravasation, and transient leg ischemia) and 2 major complications (rupture and thrombosis). CONCLUSIONS: Arterial access by recanalization of occluded segments is technically feasible in children, with a low complication rate

Experimentally engineered mutations in a ubiquitin hydrolase, UBP-1, modulate in vivo susceptibility to artemisinin and chloroquine in Plasmodium berghei

As resistance to artemisinins (current frontline drugs in malaria treatment) emerges in south East Asia, there is an urgent need to identify the genetic determinants and understand the molecular mechanisms underpinning such resistance. Such insights could lead to prospective interventions to contain resistance and prevent the eventual spread to other malaria endemic regions. Artemisinin reduced susceptibility in South East Asia (SEA) has been primarily linked to mutations in P. falciparum Kelch-13, which is currently widely recognised as a molecular marker of artemisinin resistance. However, 2 mutations in a ubiquitin hydrolase, UBP-1, have been previously associated with artemisinin reduced susceptibility in a rodent model of malaria and some cases of UBP-1 mutation variants associating with artemisinin treatment failure have been reported in Africa and SEA. In this study, we have employed CRISPR-Cas9 genome editing and pre-emptive drug pressures to test these artemisinin susceptibility associated mutations in UBP-1 in P. berghei sensitive lines in vivo. Using these approaches, we have shown that the V2721F UBP-1 mutation results in reduced artemisinin susceptibility, while the V2752F mutation results in resistance to chloroquine and moderately impacts tolerance to artemisinins. Genetic reversal of the V2752F mutation restored chloroquine sensitivity in these mutant lines while simultaneous introduction of both mutations could not be achieved and appears to be lethal. Interestingly, these mutations carry a detrimental growth defect, which would possibly explain their lack of expansion in natural infection settings. Our work has provided independent experimental evidence on the role of UBP-1 in modulating parasite responses to artemisinin and chloroquine under in vivo conditions