25 research outputs found
Vitellogenin Dynamics and Reproductive Morphology at Sexual Maturity of Philippine Mallard (Anas Platyrhynchos Domesticus L) Fed with Zinc Supplemented Diet
The vitellogenin (Vtg) is a precursor for the synthesis of egg yolk protein. Feeding with zinc-supplemented diet is hypothesized to increase the circulating Vtg thereby enhancing reproductive development. A total of 80 ducks,aged 16 weeks old, were randomly assigned to treatment groups; namely: group A with 40 ducks fed with 30 ppm zinc-supplemented diet (zinc positive) and group B with 40 ducks fed with no added zinc (zinc negative). Thecirculating Vtg at sexual maturity (155.11±10.83 days old) were determined from the blood sera. The sera were assayed for Vtg in duplicate using 96–well microplate and read the optical density at 415nm. The zinc concentrationof the samples was calculated using the nonlinear regression =OD = a x [Zn2+] / (b + [Zn2+]. Results show that the circulating Vtg in the blood sera of ducks at sexual maturity were 0.69±0.07 Gg Zn dL-1. The feeding of zinc-treateddiet had no significant influenced on the concentration of circulating Vtg. There was also insignificant difference in the reproductive morphology of ducks fed with or without added zinc. The Vtg concentration had no correlation withreproductive parameters but found to be positively correlated with liver weight (H=0.21) and negatively correlated with body weight (H = -0.24)
Arterial vascularization of the thoracic lobes of the thymus in stillborns of the lineage C40
As origens e distribuições das artérias que vascularizaram os lobos torácicos do timo foram estudadas em fetos de 30 suínos da linhagem C40, sendo 12 machos e 18 fêmeas. Os exemplares tiveram o sistema arterial preenchido com solução aquosa a 50% de Neoprene Látex corado e, em seguida, foram submetidos à fixação em solução aquosa a 10% de formaldeído. Os lobos torácicos do timo foram vascularizados por ramos diretos das artérias torácica interna direita (63,33%) e esquerda (53,33%), subclávia esquerda (3,33%), vertebral esquerda (3,33%), cervical superficial direita (3,33%) e esquerda (3,33%), carótida comum esquerda (3,33%), coronária direita (3,33%) e pelos troncos braquiocefálico (33,33%) e costocervical (3,33%). Observaram-se ainda os ramos indiretos das artérias torácica interna direita (70%) e esquerda (76,67%), subclávia esquerda (23,33%), cervical superficial esquerda (3,33%) e do arco aórtico (6,67%).The origins and distributions of the arteries that vascularization the thoracic lobes of the thymus, right and left, were studied in 30 swine of the lineage C40, fetus, being 12 males and 18 females. The animals had the arterial system filled with aqueous colored solution in a 50% of Neoprene Latex, and than were fixed in 10% aqueous solution formaldehyde. The thoracic lobes of thymus were vascularization by direct branches of the right (53.33%) and left (63.33%) internal thoracic arteries, left subclavian (3.33%), left vertebral (3.33%), right (3.33%) and left (3.33%) superficial cervical, left common carotid (3.33%), right coronary (3.33%) and brachiocephalic trunk (33.33%) and costocervical trunk (3.33%). The right internal thoracic arteries (70%) and left internal thoracic (76.67%), left subclavian (23.33%), left superficial cervical (3.33%) of the aortic arch (6.67%) provided indirect branches for the correspondent thoracic lobes of the thymus in each side
Morphology and biometry of the thymus gland in macaw of Ara and Anodorhynchus genera
Foram utilizados 12 exemplares de Ara ararauna – seis fêmeas e seis machos –, cinco exemplares de Ara chloropterus (uma fêmea e quatro machos) e dois exemplares de Anodorhynchus hyacinthinus – uma fêmea e um macho –, todos adultos, doados por criadouro particular, após óbito natural. Os lobos foram dissecados e medidos com paquímetro – comprimento x largura x espessura – e analisados quanto ao peso, à topografia e à morfologia individual. Independentemente do gênero, foram identificados, em 17 casos (89,5%), lobos tímicos nos antímeros cervicais esquerdo e direito, e em oito casos (42,1%), lobos na cavidade celomática. Os lobos apresentaram formatos alongados – 52,6% –, arredondados – 21,1% – ou ovalados – 15,8% –, posicionados preferencialmente ventromedialmente ao longo do plexo vasculoneural do pescoço, com número médio de cinco lobos por antímero, tamanho médio de 0,49cm de comprimento, 0,12cm de largura e 0,05cm de espessura e peso médio de 0,076g.Twelve samples of Ara ararauna – six females and six males –, five samples of Ara chloropterus (one female and four males) and two samples of Anodorhynchus hyacinthinus (one female and one male), all
adults from a Breeding Park, were used after natural death. The lobes were dissected and measured with
electronic calliper (length x width x thickness) and analyzed taking their weight, topography and
individual morphology into account. Regardless of gender, 89.5% of the cases presented timic lobes in
the left and right cervical antimere, and 42.1% of the cases presented lobes in the celomatic cavity. The
lobes were shown in shapes – long (52.6%), round (21.1%) or oval (15.8%), positioned mostly
ventromedially, along the neurovascular plexus of the neck, with an average of five lobes per antimere,
and an average size of 0.49cm length, 0.12cm width, and 0.05cm thick and average weight of 0.076g
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines
Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment.e FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/
2013—FP XII-BIOPLAT), Barretos Cancer Hospital, CAPES, CNPq, FAPEMIG, UFSJ. RMR is a recipient of CNPq
Productivity Gran
Vascularização arterial dos lobos cervicais do timo em fetos de suínos da linhagem C40
Vascularização arterial dos lobos torácicos do timo em fetos de suínos da Linhagem C40
Análise microscópica do miocárdio ventricular esquerdo em cães soropositivos para cinomose
Desenvolvimento vegetativo de pinhão-manso em diferentes arranjos de plantio em sistemas agrossilvipastoris
Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial
Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt