Escola Superior e Profissional de Enologia e Viticultura

Na construção existem diversas lacunas ainda durante a fase de obra ou em anomalias futuras, estas têm como principal fator a falta de conhecimentos por parte dos intervenientes. O cruzamento de informação e partilha de conhecimentos é fundamental; todos têm responsabilidade na obra construída. Para ultrapassar a falta de conhecimento, tem que haver partilha de informação e esta maioritariamente não é de fácil acesso, grande parte desta encontra-se encerrada nas instituições académicas. É urgente melhorar a comunicação para que o conhecimento se torne efetivo. O objetivo do presente trabalho é ultrapassar esta barreira da falta de comunicação, de bibliografa sobre os processos construtivos e propor algo que seja útil e que possa ajudar tanto profssionais como estudantes de arquitetura, bem como intervenientes de áreas relacionadas com a construção. A base do trabalho de investigação apresentado são as obras literárias e gráfcas analisadas, obras nacionais de referência produzidas por profssionais e empresas da área. A Proposta apresentada é uma Ficha que será a base para futuros trabalhos da unidade curricular de Acompanhamento de Obra e Fotogrametria do ISCTE – Instituto Universitário de Lisboa. A fcha é um contributo para um novo Manual de Construção e de Reabilitação evolutivo que pode e deve ser atualizado ao longo do tempo. Este manual deverá ser partilhado para dar a conhecer os materiais e processos de execução, ser de fácil leitura e interpretação por todos os estudantes e intervenientes da construção.In construction there are several gaps that occur still during the work phase or future anomalies, these have as main factor the lack of knowledge of the participants. Cross-referencing information and sharing knowledge is fundamental, all have responsibility over the constructed work. To overcome this lack of knowledge, there must be information sharing but it is not easily accessible, most is found enclosed in academical institutions. It´s urgent to improve communication so that knowledge become effective. The aim of this work is to overcome the barrier of lack of communication, lack of bibliography about construction processes and suggest something useful that may help both professionals and architecture students, as well as other participants from related areas of construction. The basis of the research presented are the literary and graphic works analyzed, national works of reference produced by professionals and companies of the area. The presented proposal is a Sheet that will be the basis for future works from the curricular unity of Acompanhamento de Obra e Fotogrametria of ISCTE – Instituto Universitário de Lisboa. The sheet is a contribution for a new evolutive Construction and Rehabilitation Manual that may and should be updated over time. This manual should be shared to introduce material and execution processes, be easily read and interpreted by all students and construction participants

Envelhecimento artificial de papel por metais e microrganismos e sua caracterização por técnicas analíticas

Envelhecimento artificial de papel por metais e microrganismos e sua caracterização por técnicas analíticas O foxing é uma descoloração do papel ou de outros materiais fibrosos e refere-se a manchas de pequenas dimensões que ocorrem de forma dispersa e que normalmente tem uma coloração alaranjada ou tonalidades entre o amarelo e o castanho-escuro. Neste trabalho, utilizaram-se quatro tipos de papel contemporâneo (papel para aguarela, cartolina duplex, papel para gravura e cartão madeira) envelhecido e não envelhecido, nos quais foram induzidas manchas de foxing com culturas mistas de fungos e com iões metálicos Fe2+ ou Cu2+ em duas concentrações diferentes, sozinhos ou em conjugação com as culturas mistas. Foi determinada a atividade celulolítica de cinco estirpes fúngicas após 24h, 48h, 72h, 96h, 168h e 336h. Para a caracterização dos papéis e das manchas induzidas foram utilizadas técnicas de análise não destrutivas, como microscopia ótica, análise colorimétrica, microscopia eletrónica de varrimento em modo de pressão variável e espectroscopia de raios-X por dispersão em energia (MEV-EDS) e espectroscopia de infravermelho com transformada de Fourier em modo de reflexão total atenuada (FT-IR-ATR); ### Abstract:Artificial aging of paper for metals and microorganisms and their characterization by analytical techniques Foxing is a discolouration of paper or other fibre-based materials and refers to small stains that occur in a dispersed and that usually have an orange color or yellowish to dark brown hues. In this work, four types of paper support (watercolour paper, duplex paperboard, gravure paper and wood cardboard) aged and non-aged were used, in which foxing stains were induced by the presence of mixed cultures of fungi obtained in papers containing foxing and by Fe2+ or Cu2+ metal ions in two different concentrations, alone or in conjunction with mixed cultures. Enzymatic activity of cellulase of five different species of fungi was analysed after 24h, 48h, 72h, 96h, 168h and 336h. For the paper characterization and induced stains were used non-destructive analysis techniques, such as light microscopy, colourimetric studies, scanning electron microscopy coupled with energy dispersive X-ray spectrometry (SEM-EDS) and ATRFourier- transformed infrared spectroscopy (ATR-FTIR)

Cistercian Pavements from Santa Maria de Alcobaça Abbey: first examples of tin-glazed tiles in Portugal

The Mosteiro de Alcobaça (Alcobaça Monastery, founded in 1153) was built to the image of Clairvaux Abbey, founded by the Cistercian abbot St. Bernard. In many ways Alcobaça mirrored the original concepts of the Cistercian Order, with an austere architectural style and shedding signs of richness. The glazed tiles found on the pavement of the deambulatory and some adjacent chapels are some of the first examples of lead-tin glazes in Christian Portugal. For the first time, these tiles were analytically characterized, to determine their chemical composition, production technology and decoration technique. The analytical techniques used were μ-PIXE (particle-induced X-ray emission), SEM-EDS (scanning electron microscopy with energy-dispersive X-ray spectrometry), and μ-Raman spectroscopy

Microglia dysfunction caused by the loss of Rhoa disrupts neuronal physiology and leads to neurodegeneration

© 2020 The Author(s). Creative Commons Attribution (CC BY 4.0)Nervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of β-amyloid plaques, and memory deficits). Mechanistically, loss of Rhoa in microglia triggers Src activation and Src-mediated tumor necrosis factor (TNF) production, leading to excitotoxic glutamate secretion. Inhibiting Src in microglia Rhoa-deficient mice attenuates microglia dysregulation and the ensuing neurological phenotype. We also find that the Rhoa/Src signaling pathway is disrupted in microglia of the APP/PS1 mouse model of Alzheimer disease and that low doses of Aβ oligomers trigger microglia neurotoxic polarization through the disruption of Rhoa-to-Src signaling. Overall, our results indicate that disturbing Rho GTPase signaling in microglia can directly cause neurodegeneration.The authors acknowledge the support of the following i3S Scientific Platforms: Animal Facility, Translational Cytometry Unit (TraCy), BioSciences Screening (BS) and Advanced Light Microscopy (ALM), and members of the national infrastructure PPBI-Portuguese Platform of BioImaging (supported by POCI-01–0145-FEDER-022122). FCT Portugal ( PTDC/MED-NEU/31318/2017-031318 ) supported work in the J.B.R. lab. FCT Portugal , PEst ( UID/NEU/04539/2013 ), COMPETE-FEDER ( POCI-01-0145-FEDER-007440 ), Centro 2020 Regional Operational Programme ( CENTRO-01-0145-FEDER-000008 : BrainHealth 2020), and Strategic Project UIDB/04539/2020 and UIDP/04539/2020 (CIBB) supported work in the A.F.A. lab. C.C.P. and R.S. hold employment contracts financed by national funds through FCT (Fundação para a Ciência e a Tecnologia, I.P.) in the context of the program contract described in paragraphs 4, 5, and 6 of article 23 of law no. 57/2016, of August 29th, as amended by law no. 57/2017 of July 19th.info:eu-repo/semantics/publishedVersio

Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi

© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian's skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2 ) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 µg/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalization (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-031158 – PTDC/BII-BIO/31158/2017. The authors would like to thank the participation and scientific support of the Unit projects UIDB/50006/2020 | UIDP/50006/2020, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universal Faixa ‘B’ (grant number 32103/2018-0). A.P. is a recipient of a post-doctoral grant from the project PTDC/BII-BIO/31158/2017. The authors would like to thank the researcher Roberto Resendes (CiBio, University of the Azores, Ponta Delgada, São Miguel, Azores, Portugal) for the logistical support in the collection of samples. C.P.A acknowledges FCT-MCTES fellowship PD/BD/136860/2018. A.B.-N. and F.C.D.A.L. acknowledge CNPq (grants 420449/2018-3 and 428211/2018-6) for financial support.info:eu-repo/semantics/publishedVersio

Analytical and microbiological characterization of 20th century photographic negatives

Two cellulose acetate photographic negatives were studied. Photography under transmitted, raking and UV illuminations in conjunction with optical microscopy, VP-SEM-EDS, ATR-FT-IR, Py-GC-MS and EDXRF were used for analytical characterization. Microbiological studies revealed the presence of three bacteria strains colonizing the negatives. Samples present a degraded status, exhibiting channels and bubbles. Triphenyl phosphate was the plasticizer used in the manufacturing process of both negatives. Cross section of one of the samples allowed to distinguish the different layers that compose the negative. Contaminants related to the manufacturing process were discussed

Portuguese adaptation of the Differentiation of Self Inventory-Revised (DSI-R): An exploratory study

A presente investigação foca-se nos estudos de adaptação e validação para a população portuguesa de um inventário de avaliação da diferenciação do self, o Differentiation of Self Inventory-Revised (DSI-R; Skowron & Schmitt, 2003). A amostra recolhida envolveu 470 adultos, sendo 249 do sexo feminino e 221 do sexo masculino, com idades compreendidas entre os 18 e os 80 anos. Nos estudos de análise fatorial exploratória da versão portuguesa não se obteve uma total replicação da composição fatorial original para os quatro fatores considerados. Os resultados obtidos para a consistência interna revelaram um valor ajustado para efeitos de investigação. A análise do impacto das variáveis sociodemográficas, no resultado total do inventário, apontou para níveis de diferenciação do self mais elevados em sujeitos mais jovens, bem como com níveis de escolaridade mais elevados. Dada a natureza exploratória desta investigação, estudos adicionais poderão reforçar as propriedades psicométricas da versão portuguesa do DSI-R.The present research focuses on the studies of adaptation and validation for the Portuguese population of an inventory which assesses the differentiation of self, the Differentiation of Self Inventory-Revised (DSI-R; Skowron & Schmitt, 2003). The sample of this research included 470 adults, 249 women and 221 men, aged between 18 and 80 years. Regarding the exploratory factor analyses for the Portuguese version, a total replication of the original factor structure for the four considered factors wasn’t obtained. The results for the internal consistency studies presented an adequate value for investigation purposes. The analysis of the impact of socio-demographic variables, on the total score of this inventory, indicated higher levels of differentiation of self in younger participants, as well as with a superior academic level. Once this is an exploratory research, additional studies could reinforce the psychometric properties of the Portuguese version of the DSI-R

Study of the materials for thermal insulation in home textiles

The thermal insulation in targeted textile substrates for home textiles assumes particular interest nowadays. Increasing performance and decreasing thickness are critical challenges to provide the required comfort to users during their rest period. Thermal insulation is associated with the phenomenon of heat absorption/desorption influencing the insulation value in fibrous material, since fibers absorb or release moisture, depending on their chemical composition. Thus, regarding thermal insulation, there are two major parameters to take into account: the conductivity and heat resistance. The main objective of this work is to study the performance of different fibrous structures for home textiles applications in terms of thermal insulation. The innovative structures considered within this study include non-woven fabrics produced with wool and hollow polyester fibers incorporating cork. Non-woven structures have been prepared in different compositions including 75%/25% wool and hollow polyester, 50%/50% wool and hollow polyester, blended with 25 mm cork granules size in proportions of 5% and 10% or with an agglomerated cork panel of 0,8mm. After production, samples were tested for their thermal behavior using Alambeta equipment. Results show that the non-woven composition influences greatly the thermal performance and the best result is obtained for sample with 50/50% wool and hollow polyester