A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly

The assembly of the embryo’s primary axis is a fundamental landmark for the establishment of the vertebrate body plan. Although the morphogenetic movements directing cell convergence towards the midline have been described extensively, little is known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that the double knockout of yap and its paralog yap1b in medaka results in an axis assembly failure, due to reduced displacement and migratory persistence in mutant cells. Accordingly, we identified genes involved in cytoskeletal organization and cell-ECM adhesion as potentially direct Yap targets. Dynamic analysis of live sensors and downstream targets reveal that Yap is acting in migratory cells, promoting cortical actin and focal adhesions recruitment. Our results indicate that Yap coordinates a mechanoregulatory program to sustain intracellular tension and maintain the directed cell migration for embryo axis development

Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network

Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors' specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR/Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted toward photoreceptor or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species

Science and tourism together in the management for the preservation of the geological heritage

El “Bosque Petrificado Florentino Ameghino” (BPFA) es la primera área protegida estatal-privada en la Provincia del Chubut que nace legalmente en el año 2010 regido bajo la figura jurídica Custodio Rural Ley XXIII N° 19. Esta es una experiencia desarrollada con bases científicas que permiten una apropiada divulgación y que se encuentra en una fase ya consolidada de formalización administrativa, educativa y turística. En cuestión de “novedad local” es una experiencia inédita en el turismo cultural al aunar interpretaciones de la Historia Geológica del lugar con la fisonomía actual de su paisaje, restos fósiles y su correlato de episodios geológicos acontecidos. Con el objetivo educativo como eje, se induce a los visitantes a observar los elementos naturales yacentes y se les ayuda a llegar a la interpretación del patrimonio en su integridad. El BPFA es por lo tanto un área protegida que posee características geológicas particulares, y fundamentalmente está siendo abordada desde una gestión integral de preservación, educación y uso sostenible, orientada al desarrollo económico y cultural local, especialmente a través del geoturismo. Se han comenzado a generar también una serie de acercamientos y acciones conjuntas de cooperación entre el BPFA y entidades civiles de la Villa Dique Florentino Ameghino, tomando como base las directrices orientativas del Servicio del Programa Geoparques de la UNESCO.The "Florentino Ameghino” Petrified Forest (FAPF) is the first state-private protected area in the Province of Chubut that was born legally in 2010 governed under the legal figure Custodio Rural Law XXIII No. 19, Chubut. This is an experience developed with scientific bases that allow an appropriate disclosure that is in an already consolidated phase of administrative, educational and tourist formalization. This project is an experience in cultural tourism as well as the interpretations of the fossils and the different geological events that occurred and the landscape. Visitors are invited to observe the geological and paleontological elements and help them to right interpretation. The FABP is a protected area that has particular geological characteristics, and is fundamentally being approached from an integral management of preservation, education and sustainable use, oriented to local economic and cultural development, especially through geo-tourism. Also, a series of joint cooperation actions between the BPFA and civil entities of Villa Dique Florentino Ameghino have begun to be generated based on the guidelines of the UNESCO Geopark Program Service.Fil: Lech, Roberto Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico de Ciencias Sociales y Humanas; ArgentinaFil: Reinoso, Jorge R.. No especifíca;Fil: Marcus, Alicia. Municipialidad de Puerto Madryn (chubut).; ArgentinaXX Congreso Geológico ArgentinoSan Miguel de TucumánArgentinaAsociación Geológica Argentin

Goats dermaphilosis in Buenos Aires province

La dermatofilosis es una dermatitis exudativa y proliferativa causada por la bacteria Dermatophilus congolensis. La enfermedad afecta a animales salvajes y domésticos e incluso al hombre. El agente causal es una bacteria Gram positiva perteneciente al orden Actinomycetales, familia Dermatophiloceae. El objetivo del trabajo fue describir la presentación de un caso de dermatofilosis en cabras. En un rodeo compuesto por 65 cabras en 14 animales se observaron las siguientes lesiones cutáneas: erupciones de pequeñas vesículas con el manto piloso agregado por el exudado, alguna de las lesiones se encontraban cubiertas por exudado desecado, localizadas en su mayoría en orejas, nariz, boca y, esporádicamente, entre las pezuñas. Se tomaron muestras para su análisis microbiológico e histopatológico. En las observaciones previas de las costras exudativas calentadas con hidróxido de potasio al 40% se pudieron observar hifas bacterianas compatibles con Dermatophilus. Se realizaron extendidos de la profundidad de las costras exudativas para ser coloreadas con los métodos de Gram y la "Tinción 15" (kit comercial), con esta última coloración se obtuvo la mejor discriminación en las formas bacterianas. Se inoculó una ansada de costras exudativas, previamente humidificadas en 1 mililitro de agua destilada estéril, en una placa de agar sangre a 37 °C. Después de 2-3 días de incubación se observó el desarrollo de colonias lisas y rugosas de color amarillento. Los cortes parafinados se colorearon con hematoxilina & eosina, ácido periódico de Schiff (PAS) y Grocott. Los filamentos bacterianos se observaron con el método de Grocott. Además de los estudios bacteriológicos y patológicos también se realizaron análisis parasitológicos en materia fecal, con resultados de 200-13.000 huevos de parásitos gastrointestinales.Dermatophilosis is an exudative to proliferative dermatitis caused by the bacterium Dermatophilus congolensis. The disease affects many animals, wild as well as domesticated and humans. The causative agent is an aerobic gram-positive bacterium belonging to the order Actinomycetales and the family Dermatophiloceae. The bacterium grows as branching filamentous mycelium. Mature filaments are composed of motile, coccal zoospores, in parallel lines, during the grow state the filaments become transversely and longitudinally separate to form parallel rows of zoospores which are dormant and can survive in the dried crusts of lesions for several months. The object of the present work was to describe an infection of dermatofilosis case in goats. From a total herd of 65 goats 14 animals had de following skin lesions: eruptions of small vesicles with the nearby hair coat matted and tufts, some were covered by crusts. Most of these mild lesions were localised in ears, nose, mouth, and sporadically in feet. Previous direct observation of exudative scab material was heat treated with 40% potasium hydroxide, bacterial hyphae resembling Dermatophilus were observed. A smear was made from de underpart of the scab material and stained by Gram stain and "Tinción 15" (commercial kit) method, this last stain accomplish for the best discrimination of bacterial shape. A loopfull of scab material placed into a tube containing 1 ml of distilled water was inoculated on to blood agar plate and incubated at 37ºC, after 2-3 days incubation period a yellowish smooth and rough colonies were observed. Paraffin sections were stained with hematoxylin & eosin, periodic acid-Schiff (PAS) and Grocott. Notorious bacterial filaments were observed with Grocott method. Beside these bacteriological and pathological studies parasites were also investigated with 200-13.000 count of gastrointestinal eggs in faeces.Facultad de Ciencias Veterinarias (FCV

Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network

Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors’ specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR/Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted toward photoreceptor or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species

Primary T-cell immunodeficiency with functional revertant somatic mosaicism in CD247

Depto. de Inmunología, Oftalmología y ORLFac. de MedicinaTRUEMinisterio de Economía y Competitividad (MINECO)Instituto de Salud Carlos III (ISCIII)Comunidad de MadridFundacion LairUniversidad Complutense de Madridpu