Cellular Imaging of Visual Cortex Reveals the Spatial and Functional Organization of Spontaneous Activity

The cerebral cortex is never silent; even in primary sensory areas there is ongoing neural activity in the absence of sensory input. Correlations in spontaneous activity can provide clues about network structure, but it has been difficult to record from enough nearby neurons to sample these correlations well. We used in vivo two-photon calcium imaging to demonstrate sparse patterns of correlated spontaneous activity among groups of ∼150 simultaneously imaged cells. In cat visual cortex, correlations fell off sharply with distance, by 50% within ∼240 μm, but in the rat there was little dependence on spatial separation up to 400 μm. In both species, cells that responded best to visual contours of a specific orientation were spontaneously co-active, suggesting that functionally related cells are organized into distinct subnetworks. Although these subnetworks are clustered in the cat, they are intermingled in the rodent, arguing for specific connections within the local cortical network

Diverse receptive fields in the lateral geniculate nucleus during thalamocortical development

articles Early studies of the effects of visual deprivation demonstrated the essential role of sensory experience in the development of the highly specific connections from thalamus to visual cortex 1 . Since then, the mechanisms underlying this refinement process have been extensively investigated, and models that attempt to explain it have been proposed The receptive-field shapes of the geniculate inputs to cortex may also be important in thalamocortical development. Most models of thalamocortical development assume geniculate inputs to be a homogeneous pool of concentric, on-or off-center cells, similar to those found in the adult The maturation of retinogeniculate connections is almost certainly the mechanism for the reduction of receptive-field size in the developing LGN The final phase of retinogeniculate maturation overlaps with the critical period for thalamocortical development. In kittens, spatial receptive-field maturation in the LGN occurs during the first 2-3 weeks after eye opening 14 . The maturation of ocular dominance columns 3 and orientation selectivity 20-24 in visual cortex occur during the first few weeks after eye opening in cats and ferrets. It is thus probable that imprecise connectivity from retina to LGN at this stage influences thalamocortical development RESULTS We recorded extracellularly from 112 neurons in layers A and A1 of the lateral geniculate nucleus of the ferret: 88 neurons in immature animals at postnatal day 31-55 (P31-P55) and 24 in adults. In addition, we recorded, in the optic tract, responses of five retinal ganglion cells from P37 and P39 animals. Eyes first open on postnatal day 30-34 in the ferret 25 . Spatial structure of developing geniculate receptive fields For all cells studied, we mapped the receptive fields with a whitenoise stimulus that consisted of 16 × 16 squares (pixels), each modulated in time by a binary temporal sequence 26 . Receptivefield maps from LGN cells in adult ferrets qualitatively resembled those obtained in previous studies of the cat LGN 27 : they were concentric and had antagonistic center/surround organization Trial-to-trial robustness of receptive-field structure To ensure that the receptive fields we mapped belonged to single cells, great care was taken to verify the quality of the recordings. Only well-isolated action potentials, as assessed by online Most models of thalamocortical development in the visual system assume a homogeneous population of thalamic inputs to the cortex, each with concentric on-or off-center receptive fields. To test this, we made high-resolution spatial maps of receptive fields in the developing ferret lateral geniculate nucleus (LGN). Developing receptive fields (RFs), had a variety of shapes: some concentric, others elongated (like adult cortical receptive fields) and some with 'hot spots' of sensitivity. These receptive fields seemed to arise from convergence of multiple retinal afferents onto LGN neurons. We present a Hebbian model whereby imprecise retinogeniculate connections help refine geniculocortical connections, sharpening both thalamocortical topography and perhaps orientation selectivity

Chronic Cellular Imaging of Entire Cortical Columns in Awake Mice Using Microprisms

SummaryTwo-photon imaging of cortical neurons in vivo has provided unique insights into the structure, function, and plasticity of cortical networks, but this method does not currently allow simultaneous imaging of neurons in the superficial and deepest cortical layers. Here, we describe a simple modification that enables simultaneous, long-term imaging of all cortical layers. Using a chronically implanted glass microprism in barrel cortex, we could image the same fluorescently labeled deep-layer pyramidal neurons across their entire somatodendritic axis for several months. We could also image visually evoked and endogenous calcium activity in hundreds of cell bodies or long-range axon terminals, across all six layers in visual cortex of awake mice. Electrophysiology and calcium imaging of evoked and endogenous activity near the prism face were consistent across days and comparable with previous observations. These experiments extend the reach of in vivo two-photon imaging to chronic, simultaneous monitoring of entire cortical columns.Video Abstrac

Galactic Center Research: Manifestations of the Central Black Hole

This review summarizes a few of the frontiers of Galactic center research that are currently the focus of considerable activity and attention. It is aimed at providing a necessarily incomplete sketch of some of the timely work being done on phenomena taking place in, or originating in, the central few parsecs of the Galaxy, with particular attention to topics related to the Galactic black hole (GBH). We have chosen to expand on the following exciting topics: 1) the characterization and the implications for the variability of emission from the GBH, 2) the strong evidence for a powerful X-ray flare in the Galactic center within the past few hundred years, and the likelihood that the GBH is implicated in that event, 3) the prospects for detecting the "shadow" of the GBH, 4) an overview of the current state of research on the central S-star cluster, and what has been learned from the stellar orbits within that cluster, and 5) the current hypotheses for the origin of the G2 dust cloud that is projected to make a close passage by the GBH in 2013.Comment: Review article, in press with Review of Astronomy and Astrophysic

Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance

SummaryAn increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity.Video Abstrac

Star Formation and Dynamics in the Galactic Centre

The centre of our Galaxy is one of the most studied and yet enigmatic places in the Universe. At a distance of about 8 kpc from our Sun, the Galactic centre (GC) is the ideal environment to study the extreme processes that take place in the vicinity of a supermassive black hole (SMBH). Despite the hostile environment, several tens of early-type stars populate the central parsec of our Galaxy. A fraction of them lie in a thin ring with mild eccentricity and inner radius ~0.04 pc, while the S-stars, i.e. the ~30 stars closest to the SMBH (<0.04 pc), have randomly oriented and highly eccentric orbits. The formation of such early-type stars has been a puzzle for a long time: molecular clouds should be tidally disrupted by the SMBH before they can fragment into stars. We review the main scenarios proposed to explain the formation and the dynamical evolution of the early-type stars in the GC. In particular, we discuss the most popular in situ scenarios (accretion disc fragmentation and molecular cloud disruption) and migration scenarios (star cluster inspiral and Hills mechanism). We focus on the most pressing challenges that must be faced to shed light on the process of star formation in the vicinity of a SMBH.Comment: 68 pages, 35 figures; invited review chapter, to be published in expanded form in Haardt, F., Gorini, V., Moschella, U. and Treves, A., 'Astrophysical Black Holes'. Lecture Notes in Physics. Springer 201

Global Patterns and Controls of Nutrient Immobilization On Decomposing Cellulose In Riverine Ecosystems

Microbes play a critical role in plant litter decomposition and influence the fate of carbon in rivers and riparian zones. When decomposing low-nutrient plant litter, microbes acquire nitrogen (N) and phosphorus (P) from the environment (i.e., nutrient immobilization), and this process is potentially sensitive to nutrient loading and changing climate. Nonetheless, environmental controls on immobilization are poorly understood because rates are also influenced by plant litter chemistry, which is coupled to the same environmental factors. Here we used a standardized, low-nutrient organic matter substrate (cotton strips) to quantify nutrient immobilization at 100 paired stream and riparian sites representing 11 biomes worldwide. Immobilization rates varied by three orders of magnitude, were greater in rivers than riparian zones, and were strongly correlated to decomposition rates. In rivers, P immobilization rates were controlled by surface water phosphate concentrations, but N immobilization rates were not related to inorganic N. The N:P of immobilized nutrients was tightly constrained to a molar ratio of 10:1 despite wide variation in surface water N:P. Immobilization rates were temperature-dependent in riparian zones but not related to temperature in rivers. However, in rivers nutrient supply ultimately controlled whether microbes could achieve the maximum expected decomposition rate at a given temperature

Nanotools for Neuroscience and Brain Activity Mapping

Neuroscience is at a crossroads. Great effort is being invested into deciphering specific neural interactions and circuits. At the same time, there exist few general theories or principles that explain brain function. We attribute this disparity, in part, to limitations in current methodologies. Traditional neurophysiological approaches record the activities of one neuron or a few neurons at a time. Neurochemical approaches focus on single neurotransmitters. Yet, there is an increasing realization that neural circuits operate at emergent levels, where the interactions between hundreds or thousands of neurons, utilizing multiple chemical transmitters, generate functional states. Brains function at the nanoscale, so tools to study brains must ultimately operate at this scale, as well. Nanoscience and nanotechnology are poised to provide a rich toolkit of novel methods to explore brain function by enabling simultaneous measurement and manipulation of activity of thousands or even millions of neurons. We and others refer to this goal as the Brain Activity Mapping Project. In this Nano Focus, we discuss how recent developments in nanoscale analysis tools and in the design and synthesis of nanomaterials have generated optical, electrical, and chemical methods that can readily be adapted for use in neuroscience. These approaches represent exciting areas of technical development and research. Moreover, unique opportunities exist for nanoscientists, nanotechnologists, and other physical scientists and engineers to contribute to tackling the challenging problems involved in understanding the fundamentals of brain function

On the mechanisms governing gas penetration into a tokamak plasma during a massive gas injection

A new 1D radial fluid code, IMAGINE, is used to simulate the penetration of gas into a tokamak plasma during a massive gas injection (MGI). The main result is that the gas is in general strongly braked as it reaches the plasma, due to mechanisms related to charge exchange and (to a smaller extent) recombination. As a result, only a fraction of the gas penetrates into the plasma. Also, a shock wave is created in the gas which propagates away from the plasma, braking and compressing the incoming gas. Simulation results are quantitatively consistent, at least in terms of orders of magnitude, with experimental data for a D 2 MGI into a JET Ohmic plasma. Simulations of MGI into the background plasma surrounding a runaway electron beam show that if the background electron density is too high, the gas may not penetrate, suggesting a possible explanation for the recent results of Reux et al in JET (2015 Nucl. Fusion 55 093013)