Análisis y desarrollo de competencias genéricas y profesionales en la universidad: un estudio empírico de los egresado

La tesis que se pretende verificar en este trabajo afirma que los egresados de la Universidad Francisco de Vitoria, por el hecho de haber cursado el Plan de Formación Humanística ofrecido a todos los estudiantes, han desarrollado una serie de competencias profesionales que pueden ser percibidas y evaluadas por sus empleadores (jefes y compañeros); asimismo, se afirma que las evaluaciones sobre la presencia de estas competencias en el desempeño profesional de los ex alumnos de la Universidad Francisco de Vitoria son más altas que las realizadas sobre el desempeño de sus compañeros de trabajo..

Herpes simplex virus type 2 infection induces AD-like neurodegeneration markers in human neuroblastoma cells

© 2015 Elsevier Inc. Herpes simplex virus (HSV) types 1 and 2 are neurotropic viruses that establish lifelong latent infections in neurons. Mounting evidence suggests that HSV-1 infection is involved in the pathogenesis of Alzheimer's disease (AD). The relationships between other herpesvirus infections and events associated with neurodegeneration have not, however, been extensively studied. The present work reports that HSV-2 infection leads to the strong accumulation of hyperphosphorylated tau and the amyloid-β peptides Aβ40 and Aβ42 (all major pathological hallmarks of AD) in human SK-N-MC neuroblastoma cells. Infection is also associated with a marked reduction in the amount of Aβ40 secreted and in the proteolytic fragments of the amyloid-β precursor protein (APP) (secreted APPα and the α-C-terminal fragment). These results indicate that HSV-2 infection inhibits the nonamyloidogenic pathway of APP processing and impairs Aβ secretion in these cells. In addition, HSV-2 induces the accumulation of intracellular autophagic compartments containing Aβ due to a failure in the late stages of autophagy. To our knowledge, this is the first report to show that HSV-2 infection strongly alters the tau phosphorylation state, APP processing, and autophagic process in human neuroblastoma cells, leading to the appearance of AD-like neurodegeneration markers.Ministerio de Economía y Competitividad (SAF2010-15558; URL: http://www.mineco.gob.es/portal/site/mineco) and Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas CIBERNED (PI2010/09-8; URL: http://www.ciberned.es).Peer Reviewe

The lysosome system is severely impaired in a cellular model of neurodegeneration induced by HSV-1 and oxidative stress

The causal agent(s) and molecular mechanisms of Alzheimer's disease (AD) remain unclear. Mounting evidence suggests that herpes simplex virus type 1 (HSV-1) infection is involved in the AD pathogenesis. Oxidative stress (OS) may also be crucial in the AD development. Our group previously reported that both HSV-1 and OS trigger the appearance of AD-type neurodegeneration markers. The main aim of the present study was to identify the mechanisms involved in this triggering. Expression studies revealed the involvement of a set of OS-regulated genes in HSV-1-infected cells and in cells harboring the Swedish mutation of the amyloid beta precursor protein gene. Functional annotation of these genes revealed the lysosome system to be impaired, suggesting that the interaction of OS with both HSV-1 and amyloid beta precursor protein mutations affects lysosomal function. Functional studies revealed HSV-1 infection and OS to increase the lysosome load, reduce the activity of lysosomal hydrolases, affect cathepsin maturation, and inhibit the endocytosis-mediated degradation of the epidermal growth factor receptor. These findings suggest alterations in the lysosome system to be involved in different forms of ADHeniike Kristen is recipient of a UAM-CSIC International Excellence Campus research contract.Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. This work was supported by the Spanish Ministerio de Ciencia e Innovación (SAF2014-53954-R

El perdón y la misericordia como condiciones de posibilidad de la paz desde diferentes enseñanzas. Propuesta de intervención educativa en la asignatura de responsabilidad social.

Pope Francis, over the years has traveled, especially, seeking reconciliation among peoples and raising the voice for the most disadvantaged and excluded. The Pope's message is one of forgiveness, reconciliation and compassion, because the Mercy of God is the hope of the world and will always be greater than our sins. Before the urgent call of Pope Francis to mercy and forgiveness we must think of young people as the witnesses of future generations who are the hope of the world. Education, according to Francisco, must be a generator of hope and considers that the role of an educator is that of a mother and a father who transmit a life full of future; hence the need for the inclusion of knowledge of the path of forgiveness towards peace in the educational task. The objective of this work is to transmit the teachings of the Pope to young people, through an educational intervention. To expand the search we have used two explanatory models of forgiveness, one exclusively focused on the teachings of Pope Francis, another, with a non-denominational nuance addressed to all people, regardless of their religious beliefs and ideas, based on the work of Gianfranco Testa, founder of the University of Perdón of Turin; In this model, Francisco's teachings have been treated as those of one more author along with other experts in the subject. The intervention was developed within the subject of Social Responsibility, second year of the degrees of Early Childhood Education, Primary Education and Physiotherapy of the Francisco de Vitoria University of Madrid (Spain). As a resource has been used to view different scenes of films in which characters and scenes are presented full of emotion and meaning in relation to the concepts developed.post-print1411 K

Matrix metalloproteinase 14 regulates HSV-1 infection in neuroblastoma cells

Growing evidence supports that chronic or latent infection of the central nervous system might be implicated in Alzheimer's disease (AD). Among them, Herpes simplex virus type 1 (HSV-1) has emerged as a major factor in the etiology of the disease. Our group is devoted to the study of the relationship among HSV-1, oxidative stress (OS) and neurodegeneration. We have found that HSV-1 induces the main neuropathological hallmarks of AD, including the accumulation of intracellular amyloid beta (Aβ), hyperphosphorylated tau protein and autophagic vesicles, that OS exacerbates these effects, and that matrix metalloproteinase 14 (MMP-14) participates in the alterations induced by OS. In this work, we focused on the role of MMP-14 in the degenerative markers raised by HSV-1 infection. Interestingly, we found that MMP-14 blockage is a potent inhibitor of HSV-1 infection efficiency, that also reduces the degeneration markers, accumulation of Aβ and hyperphosphorylated tau, induced by the virus. Our results point to MMP-14 as a potent antiviral target to control HSV-1 infection and its associated neurodegenerative effectsThis work was supported by the Ministerio de Ciencia, Innovación y Universidades (SAF2017-85747-R

Aedes albopictus in a recently invaded area in Spain: effects of trap type, locality, and season on mosquito captures

Mosquitoes are primary vectors of pathogens impacting humans, wildlife, and livestock. Among them, the Asian tiger mosquito, Aedes albopictus, stands out as an invasive species with a global distribution, having established populations on every continent except Antarctica. Recent findings incriminate Ae. albopictus in the local transmission of several pathogens causing human diseases, including dengue, chikungunya, and Zika viruses and worm parasites as Dirofilaria. In Spain, the establishment of Ae. albopictus occurred in 2004 and it rapidly expanded, currently reaching southern provinces and creating novel epidemiological scenarios in recently invaded areas. In this study, we conducted captures of Ae. albopictus from May to November 2022 in two provinces, Granada and Malaga, situated near the current edge of the species' expanding range in Spain. The objective was to identify the primary factors influencing their captures in these regions. Mosquitoes were captured using BG-Sentinel traps baited with CO2 and BG-Lure, and miniature CDC-UV traps in five different localities. Our findings underscore the influence of both extrinsic factors, such as locality, and intrinsic factors, including mosquito sex, on the abundance of captured Ae. albopictus. A higher abundance of Ae. albopictus was observed in the Malaga province compared to localities in the Granada province. Furthermore, similar numbers of Ae. albopictus mosquitoes were captured in more urbanized areas of Granada, while the lowest counts were recorded in the less urbanized area. These results were compared to captures of another common species in the area, specifically Culex pipiens. Overall, these results represent the first monitoring of invasive Ae. albopictus in the area and are discussed in the light of the potential importance of the species as a nuisance for humans and vectors of pathogens of public health relevance.This study was financed by the PID2020-118205GB-I00 grant to JMP funded by MCIN/AEI/https://doi.org/10.13039/501100011033. Additional support derived from the CNS2022-135993 grant of the Ministerio de Ciencia e Innovación (MCIN/AEI/https://doi.org/10.13039/501100011033) with funding from European Union NextGenerationEU. Mario Garrido was supported by the María Zambrano program and the P9 program for the Incorporation of Young Doctors funded by Spanish Ministry of Universities, the European Union-NextGenerationEU, and the University of Granada. Jesús Veiga received financial support from the Margarita Salas and Juan de la Cierva (FJC2021-048057-I) programs funded by Spanish Ministry of Universities, the Spanish Ministry of Science and Innovation and the European Union-NextGenerationEU. Marta Garrigós was supported by a FPI grant (PRE2021-098544). Mario Garrido is currently granted by the PID2022-137746NA-I00 funded by Spanish Ministry of Science and Innovation. We greatly appreciate the support given by the “Grupo de Investigación Comportamiento y Ecología Animal” of the University of Granada for the field sampling

In Life as in Sport: Implications of a Program to Educate Spanish Youth on Values

This research examines the effect of the Delphi Program on student self-control and self-perception. Some 305 young people (55% girls) with an average age of 11.6 years (± 0.69) enrolled in public schools in central Spain took part in the project. A quasi-experimental single-group design was used with pre-test and post-test measures following a mixed quantitative-qualitative approach. The CACIA questionnaire was used to assess students’ self-perception. A single level of treatment was conducted with groups of students in twenty didactic sessions of indoor football. The quantitative results showed an improvement in personal feedback, deferring reward, criterion self-control and process self-control. The qualitative analysis of student responses yielded four topics: conflict resolution, respect, honesty and pro-social behavior in two areas: sports and physical activity and in personal life

Functional characterization of P2Y1 and P2X4 receptors in human neuroblastoma SK-N-MC cells

Nucleotides are important signalling molecules in both the peripheral and central nervous system. However, the in vitro study of their receptors can be hampered by the heterogeneity of primary neuronal cultures. The use of clonal neuroblastoma cell lines allows to circumvent this difficulty, so these lines are often used as a model to analyze the properties, regulation and physiological role of nucleotide receptors in neural tissues. Expression studies indicated the presence of P2Y1, P2Y6, P2Y11, P2Y13, P2X1, P2X4, P2X5, P2X6 and P2X7 proteins in SK-N-MC cells. Functional analyses showed transient [Ca2+]i increases upon application of ADP, 2-MeSADP or ADPβS. Responses to these agonists seem to be mediated by a P2Y1 receptor, as demonstrated by the almost complete blockade exerted by the P2Y1-selective antagonist MRS2179. ATP was also able to induce [Ca2+]i increases in SK-N-MC cells. Responses to ATP were partially blocked by MRS2179 and the P2X antagonist TNP-ATP, thus suggesting that ATP can interact with two different P2 receptors: a P2Y1 receptor, inhibited by MRS2179, and a TNP-ATP sensitive P2X receptor. To characterize the P2X receptor responsible for the MRS2179-resistant component of the ATP response, we analyze the effect of several P2X agonists on [Ca2+]i. Cells did not show responses to either α,β-meATP or BzATP, although [Ca2+]i increases could be observed when cells were challenged with CTP. Both the response to CTP and the MRS2179-resistant component of ATP response were potentiated by ivermectin. Such pharmacological profile is consistent with the presence of a functional P2X4 receptor in SK-N-MC cell lineLos nucleótidos son importantes moléculas señalizadoras en el sistema nervioso. El estudio in vitro de sus receptores puede verse obstaculizado por la heterogeneidad de los cultivos neuronales. El uso de líneas celulares de neuroblastoma permite eludir esta dificultad y dichas líneas se utilizan frecuentemente como un modelo con el que analizar las propiedades, regulación y función de los receptores de nucleótidos en tejidos neurales. Estudios de expresión indicaron la presencia de proteínas P2Y1, P2Y6, P2Y11, P2Y13, P2X1, P2X4, P2X5, P2X6 y P2X7 en las células SK-N-MC. Análisis funcionales mostraron incrementos transitorios de [Ca2+]i tras la aplicación de ADP, 2- MeSADP o ADPβS, respuestas que parecen estar mediadas a través un receptor P2Y1, como se pone de manifiesto por el bloqueo casi total ejercido por el antagonista selectivo P2Y1, MRS2179. El ATP también indujo incrementos de [Ca2+]i en las células SK-N-MC, siendo su respuesta parcialmente bloqueada por MRS2179 y por el antagonista P2X TNP-ATP, lo que sugiere que el ATP puede interactuar con dos receptores P2 diferentes: un receptor P2Y1, inhibido por MRS2179, y un receptor P2X sensible a TNP-ATP. Se caracterizó el receptor P2X analizando el efecto de varios agonistas en la [Ca2+]i. Ninguna célula mostró respuestas a αβ- meATP o BzATP, aunque se observaron incrementos de [Ca2+]i cuando las células fueron estimuladas con CTP. Tanto la respuesta a CTP como el componente de la respuesta a ATP resistente a MRS2179, se potenciaron en presencia de ivermectina. Todos estos datos sugieren la presencia de un receptor P2X4 funcional en las células SK-N-MCThis work was supported by research grants from Ministerio de Ciencia e Innovación (BFU2014-53654-P) and Comunidad de Madrid (S2013/ICE-2958 BRADECM

Monitoring of bluetongue virus in zoo animals in Spain, 2007–2019

Bluetongue (BT) is an emerging and re-emerging communicable vector-borne disease of animal health concern. A serosurvey was performed to assess exposure to BT virus (BTV) in zoo animals in Spain and to determine the dynamics of seropositivity in longitudinally sampled individuals during the study period. Serum samples were collected from 241 zoo animals belonging to 71 different species in five urban zoos (A-E) in Spain between 2007 and 2019. Twenty-four of these animals were longitudinally surveyed at three of the sampled zoos (zoos B, C and E) during the study period. Anti-BTV antibodies were found in 46 (19.1%; 95% CI: 14.1-24.1) of the 241 captive animals analysed by commercial ELISA. A virus neutralization test confirmed specific antibodies against BTV-1 and BTV-4 in 25 (10.7%; 95% CI: 6.7-14.6) and five (3.0%; 95% CI: 0.3-4.0) animals, respectively. Two of the 24 longitudinally sampled individuals (one African elephant (Loxodanta africana) and one aoudad (Ammotragus lervia)) showed anti-BTV antibodies at all samplings, whereas seroconversions were detected in one mouflon (Ovis aries musimon) in 2016, and one Asian elephant (Elephas maximus) in 2019. To the best of the authors' knowledge, this is the first large-scale survey on BTV conducted in both artiodactyl and non-artiodactyl zoo species worldwide. The results confirm BTV exposure in urban zoo parks in Spain, which could be of animal health and conservation concern. Circulation of BTV was detected in yearling animals in years when there were no reports of BTV outbreaks in livestock. Surveillance in artiodactyl and non-artiodactyl zoo species could be a valuable tool for epidemiological monitoring of BTV.info:eu-repo/semantics/acceptedVersio