Transverse electric field dragging of DNA in a nanochannel

Nanopore analysis is an emerging single-molecule strategy for non-optical and high-throughput DNA sequencing, the principle of which is based on identification of each constituent nucleobase by measuring trans-membrane ionic current blockade or transverse tunnelling current as it moves through the pore. A crucial issue for nanopore sequencing is the fact that DNA translocates a nanopore too fast for addressing sequence with a single base resolution. Here we report that a transverse electric field can be used to slow down the translocation. We find 400-fold decrease in the DNA translocation speed by adding a transverse field of 10 mV/nm in a gold-electrode-embedded silicon dioxide channel. The retarded flow allowed us to map the local folding pattern in individual DNA from trans-pore ionic current profiles. This field dragging approach may provide a new way to control the polynucleotide translocation kinetics

The Stop Rag

Illustration of train travelling on tracks and red flag wavinghttps://scholarsjunction.msstate.edu/cht-sheet-music/3728/thumbnail.jp

The "stop" rag

Gift of Dr. Mary Jane Esplen.Piano [instrumentation]F major [key]Not too fast [tempo]Ragtime piano music [form/genre]Train, track, flag [illustration]Publisher's advertisement on back cover [note

The Stop rag: a Southern buck dance

(Published By A.F. Marzian

Milrinone as a rescue therapy for symptomatic refractory cerebral vasospasm in aneurysmal subarachnoid hemorrhage

Introduction: Delayed ischemic neurological deficit associated to cerebral vasospasm is the most common cause of sequelae and death that follows the rupture of an aneurysm. The objective of this study was to evaluate the safety and efficacy of intra-arterial Milrinone in patients with symptomatic refractory cerebral vasospasm. Patients and Method: Eight patients diagnosed with aneurysmal subarachnoid hemorrhage who developed symptomatic cerebral vasospasm refractory to conventional medical therapy were enrolled. They received an intra-arterial infusion of Milrinone at a rate of 0.25 mg/min, with a total dose of 10-15 mg. Qualitative evaluation of angiographic response, neurological and systemic complications as well as functional outcome at 3 months were documented. Results: All patients had a significant angiographic response. This was evidenced by a pre-treatment vessel stenosis greater than 70%, that improved to less than 50% after the intra-arterial Milrinone infusion. Three patie

COGNITIVE PERFORMANCE IN PATIENTS WITH CORONARY HEART DISEASE AND CARDIOVASCULAR RISK FACTORS IN CHILE

Introducción. La presencia de factores de riesgo cardiovascular (FRCV) se relaciona con una peor evolución cognitiva a largo plazo. La cardiopatía coronaria (CC) se ha asociado inconstantemente a mala evolución cognitiva. Objetivos. Se trata de evaluar el rendimiento cognitivo de pacientes chilenos con distintos grados de FRCV, con y sin CC. Sujetos y métodos. Es un estudio transversal de evaluación cognitiva en tres grupos de pacientes mayores de 60 años sin antecedentes de ataques cerebrovasculares: con CC y FRCV elevados (CC-FRCVE) (n = 62), con FRCV elevados sin CC (FRCVE) (n = 57), y sin antecedentes de hipertensión arterial (HTA), diabetes ni CC o ‘no hipertenso no diabético’ (NHND) (n = 25). Resultados. En la totalidad de la muestra los factores asociados significativamente a deterioro cognitivo fueron: mayor edad, menor escolaridad y ser hombre. No hubo diferencias significativas en la edad ni en la proporción de pacientes con deterioro cognitivo entre los tres grupos; los NHND tenían significativamente mayor escolaridad que los otros grupos. Los CC-FRCVE tuvieron mayor proporción de hombres y mayor proporción de enfermedad arterial oclusiva periférica y dislipidemia que los otros grupos. Los FRCVE tuvieron significativamente mayor cantidad de años con HTA que los otros grupos. En las escalas cognitivas y de depresión, no se encontraron diferencias estadísticamente significativas en el rendimiento entre los tres grupos después de ajustar los resultados por escolaridad y sexo, y existió significativamente mayor dependencia funcional en el grupo coronario. Conclusiones. La CC en pacientes sin antecedentes de enfermedad cerebrovascular, en forma aislada, no se asocia a peor rendimiento cognitivo global, pero sí a mayor daño vascular sistémico y mayor dependencia funcional