The Best of Both Worlds: Connecting Remote Sensing and Arctic Communities for Safe Sea Ice Travel

  Northern communities are increasingly interested in technology that provides information about the sea ice environment for travel purposes. Synthetic aperture radar (SAR) remote sensing is widely used to observe sea ice independently of sunlight and cloud cover, however, access to SAR in northern communities has been limited. This study 1) defines the sea ice features that influence travel for two communities in the Western Canadian Arctic, 2) identifies the utility of SAR for enhancing mobility and safety while traversing environments with these features, and 3) describes methods for sharing SAR-based maps. Three field seasons (spring and fall 2017 and spring 2018) were used to engage residents in locally guided research, where applied outputs were evaluated by community members. We found that SAR image data inform and improve sea ice safety, trafficability, and education. Information from technology is desired to complement Inuit knowledge-based understanding of sea ice features, including surface roughness, thin sea ice, early and late season conditions, slush and water on sea ice, sea ice encountered by boats, and ice discontinuities. Floe edge information was not a priority. Sea ice surface roughness was identified as the main condition where benefits to trafficability from SAR-based mapping were regarded as substantial. Classified roughness maps are designed using thresholds representing domains of sea ice surface roughness (smooth ice/maniqtuk hiku, moderately rough ice/maniilrulik hiku, rough ice/maniittuq hiku; dialect is Inuinnaqtun). These maps show excellent agreement with local observations. Overall, SAR-based maps tailored for on-ice use are beneficial for and desired by northern community residents, and we recommend that high-resolution products be routinely made available in communities.  Les collectivités du Nord s’intéressent de plus en plus aux technologies qui leur fournissent de l’information au sujet de l’environnement de glace de mer à des fins de déplacements. La télédétection par radar à synthèse d’ouverture (SAR) est couramment utilisée pour observer la glace de mer, indépendamment de la lumière du soleil et de la nébulosité. Cependant, dans les collectivités du Nord, l’accès au SAR est restreint. Cette étude 1) définit les caractéristiques de la glace de mer qui exercent une influence sur les déplacements de deux collectivités dans l’ouest de l’Arctique canadien; 2) détermine l’utilité du SAR pour améliorer la mobilité et la sécurité quand vient le temps de traverser des environnements comportant ces caractéristiques; et 3) décrit les méthodes de partage de cartes établies à l’aide du SAR. Trois saisons sur le terrain (le printemps et l’automne de 2017, et le printemps de 2018) ont permis d’inciter les résidents à participer à une recherche locale guidée, là où les extrants appliqués ont été évalués par les membres de la collectivité. Nous avons trouvé que les données émanant des images du SAR éclairent et améliorent la sécurité de la glace de mer, l’aptitude à la circulation et l’éducation. L’information découlant de la technologie s’avère un complément désirable aux connaissances inuites en vue de la compréhension des caractéristiques de la glace de mer, dont la rugosité de la surface, la glace de mer mince, les conditions en début et en fin de saison, la bouillie de glace et la glace mouillée, la glace de mer rencontrée par les bateaux, et la discontinuité de la glace. Les données sur la glace de banc ne constituaient pas une priorité. La rugosité de la surface de la glace de mer était considérée comme la principale condition pour laquelle les avantages de la praticabilité déterminés au moyen des cartes établies à l’aide du SAR étaient substantiels. Les cartes indiquant la rugosité sont conçues en fonction de seuils représentant les caractéristiques de rugosité de la surface des glaces de mer (glace lisse/maniqtuk hiku, glace modérément rugueuse/maniilrulik hiku, glace rugueuse/maniittuq hiku; en dialecte inuinnaqtun). Ces cartes sont largement en accord avec les observations locales. Dans l’ensemble, les cartes établies à l’aide du SAR préparées en fonction des utilisations de la glace sont bénéfiques et désirées par les résidents des collectivités du Nord. Nous recommandons que des produits de haute résolution soient régulièrement mis à la disposition des collectivités

Changes in EMG and Finger Force with Repeated Hangs from the Hands in Rock Climbers

The nature and degree of fatigue in muscles that control finger position during repeated sustained efforts in rock climbing have not been described. The purpose of this study was to identify changes in maximum hang time and forearm electromyogram (EMG) during repeated maximum duration hangs from a simulated rock feature. A second objective was to determine the effect of different recovery times between hangs upon changes in finger force. Five experienced rock climbers performed 2 test sessions on different days in a randomized order. Each session involved 8 repetitions of a maximum duration hang with either 1 min (R1) or 3 min (R3) resting recovery between hangs. Finger force (FF) was measured for the right hand via a piezoelectric force sensor fitted with a plate to accept the distal digits of four fingers. Peak EMG (EMGPK) and EMG Area (EMGAREA) were recorded from the anterior forearm for each hang and standardized as percent of maximum FF EMG prior to statistical analysis. Hang duration progressively decreased over repetitions but tended to plateau around repetition 5 for both R1 and R3 conditions. A significant difference was found for both recovery conditions and repetitions (p\u3c.05) as well as a significant interaction of the two factors (p\u3c.05) for hang duration. There were no significant differences for EMGPK among repetitions or between recovery conditions (p\u3e.05). EMGAREA decreased initially with repeated hangs during both R1 and R3 but this trend tended to plateau at repetition 3 for the R3 condition. A significant difference was found in EMGAREA for both recovery conditions and trials (p\u3c.05) as well as a significant interaction of the two factors (p\u3c.05). Mean FF decreased between pre- and post-hangs for both R1 and R3 however the difference was not significant. It was concluded that the overall decline in hang time is less with 3 min recovery vs 1 min recovery between hangs. Peak EMG does not appear to change despite this evidence of fatigue. A 3 min recovery interval between hang repetitions decreases the magnitude of fatigue experienced and enables a greater EMGAREA per hang

Examination of the Potential Association of Stress with Morbidity and Mortality Outcomes in Patient with Heart Failure

OBJECTIVES: The high mortality and morbidity rates associated with heart failure are still not well explained. A few psychosocial factors have been studied and explain some of this risk, but other factors, like stress, remain largely unexplored in heart failure. This study aimed to (1) examine the association of stress with 6-month cardiac event-free survival, (2) examine the relationship of stress with salivary cortisol, and (3) examine the association of salivary cortisol level with 6-month cardiac event-free survival. METHOD: A total of 81 heart failure patients participated. Stress was measured using the brief Perceived Stress Scale. Cortisol was measured from unstimulated whole expectorated saliva. Cox regression analyses were used to determine whether stress predicted event-free survival, and if salivary cortisol predicted event-free survival. Linear and multiple regressions were used to determine the association of stress with salivary cortisol. RESULTS: Stress was not a significant predictor of event-free survival in heart failure (heart rate = 1.06; 95% confidence interval = 0.95-1.81; p = 0.32). Salivary cortisol was a significant predictor of event-free survival in the unadjusted model (heart rate = 2.30; 95% confidence interval = 0.99-5.927; p = 0.05), but not in the adjusted model. Stress (β 1.06; 95% confidence interval = 0.95-1.18; p = 0.32) was not a significant predictor of salivary cortisol level. CONCLUSION: Stress is a complex phenomenon, and our measure of stress may not have captured it well. Alternatively, the physical stressors acting in heart failure produce levels of neurohormonal activation that mask the effects of psychosocial stressors or an indirect association of stress with outcomes that is mediated through another construct. Future studies are needed to investigate stress in patients with heart failure to provide definitive answers

Platinum bis(phosphine) complexes of 1,8-naphthosultam

The work in this project was supported by the Engineering and Physical Sciences Research Council (EPSRC).A series of bis(phosphine) platinum complexes 1-4 and 6-8 that bear the 1,8-naphthosultam ligand (L) have been synthesised. The nitrogen atom in L was deprotonated with sodium tert-butoxide to form the sodium salt. Metathetical reaction of the sodium salt (1 eq.) with the appropriate cis-dichlorobis(phosphine) platinum (1 eq.) in THF resulted in the formation of platinum complexes [Pt(PR3)2(L)Cl] (R3 = Ph3; 1, Ph2Me; 2, PhMe2; 3, Me3; 4), whilst reaction with [Pt(COD)Cl2] afforded [Pt(COD)(L)Cl] (5). The corresponding reaction employing two equivalents of L, two equivalents of NaOtBu and one equivalent of [Pt(PR3)2Cl2]/[Pt(COD)Cl2] yielded complexes [Pt(PR3)2(L)2] (R3 = Ph2Me; 6, PhMe2; 7, Me3; 8) and [Pt(COD)(L)2] (9). L, 1, 5 and 9 have been fully characterised, principally by multinuclear magnetic resonance and IR spectroscopy and mass spectrometry, the remaining members of the series were analysed by 31P NMR only. Unsymmetrical complexes 1-4 provide examples of AX spin systems, with appropriate satellites attributed to 31P NMR-195Pt coupling. X-ray structures determined for 1,8-naphthosultam L, and complexes 1, 5 and 9 and where appropriate the platinum metal geometry, N-S distance and ligand distortions were compared.PostprintPeer reviewe

Farmers Markets and the Local Food System: The Case of Gettysburg, Pennsylvania

In order to examine and obtain a better understanding of the local food system within Adams County, Pennsylvania, this study explores the characteristics and perspectives of the customers and vendors at the farmers markets in Gettysburg, Pennsylvania. Survey findings from the Gettysburg Farmers Market and the three Adams County Farmers Markets include customer demographic information, perspectives and shopping behavior as well as vendor product information, farm size and location and preference for market management. Introductory background information on the Farm Bill and the influence of agricultural practices on the environment, human health and nutrition and the relationship between farmers markets and the local economy are offered in order to emphasize the value of a well-managed local food system. Conclusions provide evidence that lower income and lower education levels are not sufficiently represented at all the markets and food stamp programs are being underutilized. This study suggests employing additional marketing to target underrepresented demographic groups, public transportation to potentially inaccessible market locations and increased advertisement and encouragement of food stamp programs at all markets in order to expand the customer base and increase access to healthy, local foods for less advantaged citizens. The results from this study are intended to offer evidence that will promote and facilitate market management, strengthen customer/vendor relationships and encourage better ties between the local community and local food systems at the farmers markets within Gettysburg in Adams County, Pennsylvania

Factors influencing malaria control policy-making in Kenya, Uganda and Tanzania

Abstract Background Policy decisions for malaria control are often difficult to make as decision-makers have to carefully consider an array of options and respond to the needs of a large number of stakeholders. This study assessed the factors and specific objectives that influence malaria control policy decisions, as a crucial first step towards developing an inclusive malaria decision analysis support tool (MDAST). Methods Country-specific stakeholder engagement activities using structured questionnaires were carried out in Kenya, Uganda and Tanzania. The survey respondents were drawn from a non-random purposeful sample of stakeholders, targeting individuals in ministries and non-governmental organizations whose policy decisions and actions are likely to have an impact on the status of malaria. Summary statistics across the three countries are presented in aggregate. Results Important findings aggregated across countries included a belief that donor preferences and agendas were exerting too much influence on malaria policies in the countries. Respondents on average also thought that some relevant objectives such as engaging members of parliament by the agency responsible for malaria control in a particular country were not being given enough consideration in malaria decision-making. Factors found to influence decisions regarding specific malaria control strategies included donor agendas, costs, effectiveness of interventions, health and environmental impacts, compliance and/acceptance, financial sustainability, and vector resistance to insecticides. Conclusion Malaria control decision-makers in Kenya, Uganda and Tanzania take into account health and environmental impacts as well as cost implications of different intervention strategies. Further engagement of government legislators and other policy makers is needed in order to increase funding from domestic sources, reduce donor dependence, sustain interventions and consolidate current gains in malaria.http://deepblue.lib.umich.edu/bitstream/2027.42/109455/1/12936_2014_Article_3344.pd

Representing kidney development using the gene ontology.

Gene Ontology (GO) provides dynamic controlled vocabularies to aid in the description of the functional biological attributes and subcellular locations of gene products from all taxonomic groups (www.geneontology.org). Here we describe collaboration between the renal biomedical research community and the GO Consortium to improve the quality and quantity of GO terms describing renal development. In the associated annotation activity, the new and revised terms were associated with gene products involved in renal development and function. This project resulted in a total of 522 GO terms being added to the ontology and the creation of approximately 9,600 kidney-related GO term associations to 940 UniProt Knowledgebase (UniProtKB) entries, covering 66 taxonomic groups. We demonstrate the impact of these improvements on the interpretation of GO term analyses performed on genes differentially expressed in kidney glomeruli affected by diabetic nephropathy. In summary, we have produced a resource that can be utilized in the interpretation of data from small- and large-scale experiments investigating molecular mechanisms of kidney function and development and thereby help towards alleviating renal disease

RGS12 and RGS14 GoLoco Motifs Are Gα i Interaction Sites with Guanine Nucleotide Dissociation Inhibitor Activity

The regulators of G-protein signaling (RGS) proteins accelerate the intrinsic guanosine triphosphatase activity of heterotrimeric G-protein alpha subunits and are thus recognized as key modulators of G-protein-coupled receptor signaling. RGS12 and RGS14 contain not only the hallmark RGS box responsible for GTPase-accelerating activity but also a single G alpha(i/o)-Loco (GoLoco) motif predicted to represent a second G alpha interaction site. Here, we describe functional characterization of the GoLoco motif regions of RGS12 and RGS14. Both regions interact exclusively with G alpha(i1), G alpha(i2), and G alpha(i3) in their GDP-bound forms. In GTP gamma S binding assays, both regions exhibit guanine nucleotide dissociation inhibitor (GDI) activity, inhibiting the rate of exchange of GDP for GTP by G alpha(i1). Both regions also stabilize G alpha(i1) in its GDP-bound form, inhibiting the increase in intrinsic tryptophan fluorescence stimulated by AlF(4)(-). Our results indicate that both RGS12 and RGS14 harbor two distinctly different G alpha interaction sites: a previously recognized N-terminal RGS box possessing G alpha(i/o) GAP activity and a C-terminal GoLoco region exhibiting G alpha(i) GDI activity. The presence of two, independent G alpha interaction sites suggests that RGS12 and RGS14 participate in a complex coordination of G-protein signaling beyond simple G alpha GAP activity

Portable, Battery-Operated, Low-Cost, Bright Field and Fluorescence Microscope

This study describes the design and evaluation of a portable bright-field and fluorescence microscope that can be manufactured for $240 USD. The microscope uses a battery-operated LED-based flashlight as the light source and achieves a resolution of 0.8 µm at 1000× magnification in fluorescence mode. We tested the diagnostic capability of this new instrument to identify infections caused by the human pathogen, Mycobacterium tuberculosis. Sixty-four direct, decontaminated, and serially diluted smears were prepared from sputa obtained from 19 patients suspected to have M. tuberculosis infection. Slides were stained with auramine orange and evaluated as being positive or negative for M. tuberculosis with both the new portable fluorescence microscope and a laboratory grade fluorescence microscope. Concordant results were obtained in 98.4% of cases. This highly portable, low cost, fluorescence microscope may be a useful diagnostic tool to expand the availability of M. tuberculosis testing at the point-of-care in low resource settings