Surface grid generation for complex three-dimensional geometries

An outline is presented for the creation of surface grids from primitive geometry data such as obtained from CAD/CAM systems. The general procedure is applicable to any geometry including full aircraft with wing, nacelle, and empennage. When developed in an interactive graphics environment, a code based on this procedure is expected to substantially improve the turn around time for generating surface grids on complex geometries. Results are shown for a general hypersonic airplane geometry

Development of computerized analysis for solid propellant combustion (ISAP-2)

This report is an improvement of ISAP-1, SRB Vorticity-Acoustic Coupled Instability Analysis, September 1986. Included in this report are the automatic generation of all input data for grid configuration, boundary conditions for coupled acoustic and vortical field calculations, transformation of all dimensions to a parametric form, resulting in flexibility for the user to define the size of the problem (geometric configurations) with reduction in storage (15 to 65%) and computer run-time (50 to 75%). Additional research is required for the following areas: (1) turbulence effects; (2) nonlinear wave oscillations; and (3) chemistry upon combustion instability

A checklist of the hepatics and anthocerotes of Panamá

A brief account of the geography and floristic regions of Panamá is complemented with an historical account of the liverwort studies of this country. This is followed by an annotated checklist of 289 taxa, including 93 new for Panamá. Among these are four hornwort taxa that represent the first anthocerotes reported. Each checklist entry comprises proper taxon citation, collection data and/or bibliographic references.Una revisión de la geografía y regiones florísticas de Panamá es complementada con una nota histórica de los estudios sobre hepáticas de este país. Esto es seguido por una lista anotada de 289 taxa, incluyendo 93 que son nuevos para Panamá. Entre éstos se encuentran cuatro antocerotes que representan las primeras comunicaciones en esta División. Cada taxon ingresado contiene la citación apropiada, datos de colecta y/o referencias bibliográficas

Hepatitis C Virus and Hepatocarcinogenesis

Hepatitis C virus (HCV) is an RNA virus that is unable to integrate into the host genome. However, its proteins interact with various host proteins and induce host responses. The oncogenic process of HCV infection is slow and insidious and probably requires multiple steps of genetic and epigenetic alterations, the activation of cellular oncogenes, the inactivation of tumor suppressor genes, and dysregulation of multiple signal transduction pathways. Stellate cells may transdifferentiate into progenitor cells and possibly be linked to the development of hepatocellular carcinoma (HCC). Viral proteins also have been implicated in several cellular signal transduction pathways that affect cell survival, proliferation, migration and transformation. Current advances in gene expression profile and selective messenger RNA analysis have improved approach to the pathogenesis of HCC. The heterogeneity of genetic events observed in HCV-related HCCs has suggested that complex mechanisms underlie malignant transformation induced by HCV infection. Considering the complexity and heterogeneity of HCCs of both etiological and genetic aspects, further molecular classification is required and an understanding of these molecular complexities may provide the opportunity for effective chemoprevention and personalized therapy for HCV-related HCC patients in the future. In this review, we summarize the current knowledge of the mechanisms of hepatocarcinogenesis induced by HCV infection

Maintenance cost studies of rotary wing commercial transport aircraft

December 1974Includes bibliographical references (p. 121-123)Introduction: The vertical take-off and landing (VTOL) aircraft market has had substantial growth in the period of the last ten years when one considers the overall number of aircraft in use. The military fleet has continued to increase, as have such operators as natural resource (petroleum and lumber) companies, and law enforcement agencies. (See Table 1.) In scheduled passenger service, however, the VTOL- market has not enjoyed sustained growth. Consider Table 2, the type and number of helicopters in passenger service during 1962-1972. Following the cessation of federal subsidies to helicopter operators in 1966 the number of aircraft (and total available seats) has been steadily declining. Table 3 shows the composition of the fleets of the certificated carriers since 1966. Los Angeles Airways has been in bankruptcy since 1969; Chicago Helicopter is now largely a charter operator, although retaining its certificate; New York Airways, after a period of experimentation with the fixed wing Twin Otter (DHC-6) in 1968-1969, finally made it into the black in 1973, flying Sikorsky S-61's; and SFO Helicopter has retrenched its passenger services severely, but is not yet profitable. Why is the state of scheduled passenger operations so bleak? Many answers to this question have been given. For example, it has been said that the aircraft used by the operators have been inadequate: that they have been designed for military use and are ill suited for civilians who have been used to a higher comfort level (especially since most flights taken on a helicopter are in conjunction with a ride on a large, comfortable jet transport). Alternatively, it has been said that the high cost of operating the current helicopter fleet has caused the ticket price to be too high to be attractive to the traveler. Sometimes the operators have been fingered as the culprits -- that they have not priced their product adequately and have structured their networks poorly, i.e., that the failure has been one of management and marketing. And from the purely technology minded, the answer has been that once the properly designed rotary wing aircraft arrives on the scene -- one designed for civilian use and having the proper payload-range configuration -- the market will boom as VTOL aircraft enter city-center to city-center service. Doubtless there is a kernel -of truth in all these explanations, and examples to sustain most of them can be found in the history of helicopter operations in the United States. The intent of the work described in this report was to explore one frequently cited cause of the problem of high operating costs of helicopters in scheduled service - to wit, high maintenance costs of rotary wing aircraft. This attempt was made to allow a look ahead and to predict trends in maintenance costs of future rotary wing aircraft.This work was performed under a NASA Contract for Ames Research Cente

CCAAT/Enhancer-Binding Protein Homologous (CHOP) Protein Promotes Carcinogenesis in the DEN-Induced Hepatocellular Carcinoma Model

Background and Aims C/EBP homologous protein (CHOP) plays pro-apoptotic roles in the integrated stress response. Recently, a tumor suppressive role for CHOP was demonstrated in lung cancer via regulation of tumor metabolism. To explore the role of CHOP in hepatocarcinogenesis, we induced hepatocellular carcinoma (HCC) in wild type (wt) and CHOP knockout (KO) mice using the carcinogen N-diethylnitrosamine (DEN). Results: Analysis of tumor development showed reduced tumor load, with markedly smaller tumor nodules in the CHOP KO animals, suggesting oncogenic roles of CHOP in carcinogen-induced HCC. In wt tumors, CHOP was exclusively expressed in tumor tissue, with minimal expression in normal parenchyma. Analysis of human adenocarcinomas of various origins demonstrated scattered expression of CHOP in the tumors, pointing to relevance in human pathology. Characterization of pathways that may contribute to preferential expression of CHOP in the tumor identified ATF6 as a potential candidate. ATF6, a key member of the endoplasmic reticulum stress signaling machinery, exhibited a similar pattern of expression as CHOP and strong activation in wt but not CHOP KO tumors. Because HCC is induced by chronic inflammation, we assessed whether CHOP deficiency affects tumor-immune system crosstalk. We found that the number of macrophages and levels of IFNγ and CCL4 mRNA were markedly reduced in tumors from CHOP KO relative to wt mice, suggesting a role for CHOP in modulating tumor microenvironment and macrophage recruitment to the tumor. Conclusion: Our data highlights a role for CHOP as a positive regulator of carcinogen-induced HCC progression through a complex mechanism that involves the immune system and modulation of stress signaling pathways

Nonalcoholic steatohepatitis is associated with an atherogenic lipoprotein subfraction profile

Background: Nonalcoholic steatohepatitis (NASH) carries an increased risk of cardiovascular disease (CVD) relative to the general population. We sought to evaluate whether differences in lipoprotein subfractions in obese patients with and without NASH contributes to this difference in CVD risk. Findings: Ion mobility analysis was performed on 78 individuals with obesity undergoing weight loss surgery. All individuals had standard of care liver biopsies performed during surgery. Patients with NASH had significantly smaller peak LDL diameter (P = 0.02, 219.0 Å vs. 222.6 Å), and levels of IDL2 (P = 0.01, 104. nmol/L vs. 133.4 nmol/L) and HDL2b (P = 0.05, 676.7 nmol/L vs. 880.1 nmol/L) compared to those without NASH. NASH patients had significantly higher LDL-IVb levels than those without NASH (P = 0.02, 49.0 nmol/L vs. 37.1 nmol/L). The inverse association of LDL peak diameter with NASH remained significant after adjustment for diabetes (P = 0.02). HDL2b levels were inversely correlated with hepatocyte ballooning and NASH and these remained significant after adjustment for diabetes (P = 0.0017 and P = 0.007, respectively). IDL2 levels were inversely correlated with NASH, hepatocyte ballooning and fibrosis stage but these were not significant after adjustment for diabetes. Conclusions: The lipoprotein subfraction profile in subjects with NASH is characterized by small peak LDL diameter, reduced HDL2b levels and elevated LDL-IVb levels. These changes may contribute to the increased CVD seen in patients with NASH

Inhibition of HCV by the serpin antithrombin III

Background: Although there have been dramatic strides made recently in the treatment of chronic hepatitis C virus infection, interferon-α based therapy remains challenging for certain populations, including those with unfavorable IL28B genotypes, psychiatric co-morbidity, HIV co-infection, and decompensated liver disease. We have recently shown that ATIII, a serine protease inhibitor (serpin), has broad antiviral properties. Results: We now show that ATIII is capable of inhibiting HCV in the OR6 replicon model at micromolar concentrations. At a mechanistic level using gene-expression arrays, we found that ATIII treatment down-regulated multiple host cell signal transduction factors involved in the pathogenesis of cirrhosis and hepatocellular carcinoma, including Jun, Myc and BMP2. Using a protein interactive network analysis we found that changes in gene-expression caused by ATIII were dependent on three nodes previously implicated in HCV disease progression or HCV replication: NFκB, P38 MAPK, and ERK1/2. Conclusions: Our findings suggest that ATIII stimulates a novel innate antiviral host cell defense different from current treatment options

The Absence of Obstructive Sleep Apnea May Protect against Non-Alcoholic Fatty Liver in Patients Undergoing Bariatric Surgery

Background: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease worldwide and its progressive form, steatohepatitis, will be the leading indication for liver transplant by 2020. While risk factors for steatohepatitis have been identified, little work has been performed to identify factors protective against NAFLD development. Aim This study sought to identify factors predictive of normal liver histology in a bariatric cohort. Methods: Patients undergoing weight loss surgery with liver biopsies at the time of surgery were included. Patients with other causes of chronic liver disease were excluded. Results: One hundred fifty-nine patients were included. Forty-nine patients had normal liver histology and 110 patients had NAFLD. Several previously identified factors associated with normal liver histology were found. Black race was the strongest predictor of the absence of NAFLD with an odds ratio (OR) of 6.8, 95% confidence interval (CI) 2.4–18.9. Low HOMA-IR was also associated with normal histology (OR 1.4, 95% CI 1.03–1.9). In contrast, low HDL was associated with a decreased chance of normal histology (OR 0.38, 95% CI 0.05–0.83). Interestingly, a novel protective factor, the absence of obstructive sleep apnea (OSA) was strongly associated with normal histology (OR 5.6, 95% CI 2.0–16.1). In multivariate regression controlling for BMI, black race, absence of OSA, low HOMA-IR and low ALT independently predicted normal liver histology with an area under the ROC curve of 0.85. Conclusions: Our study confirmed several factors associated with normal liver histology, including black race and identified a novel factor, absence of OSA. Further evaluation of these factors will allow for improved understanding of the pathogenesis of NAFLD