2,034 research outputs found
Reprocessing Single-Use Medical Devices: The State of the Debate
Reprocessing single-use medical devices is a growing but controversial industry subject to an increasingly complex and fast-developing set of regulations and a wide range of unresolved legal issues
Bcl-xL-mediated remodeling of rod and cone synaptic mitochondria after postnatal lead exposure: electron microscopy, tomography and oxygen consumption.
PurposePostnatal lead exposure produces rod-selective and Bax-mediated apoptosis, decreased scotopic electroretinograms (ERGs), and scotopic and mesopic vision deficits in humans and/or experimental animals. Rod, but not cone, inner segment mitochondria were considered the primary site of action. However, photoreceptor synaptic mitochondria were not examined. Thus, our experiments investigated the structural and functional effects of environmentally relevant postnatal lead exposure on rod spherule and cone pedicle mitochondria and whether Bcl-xL overexpression provided neuroprotection.MethodsC57BL/6N mice pups were exposed to lead only during lactation via dams drinking water containing lead acetate. The blood [Pb] at weaning was 20.6±4.7 ”g/dl, which decreased to the control value by 2 months. To assess synaptic mitochondrial structural differences and vulnerability to lead exposure, wild-type and transgenic mice overexpressing Bcl-xL in photoreceptors were used. Electron microscopy, three-dimensional electron tomography, and retinal and photoreceptor synaptic terminal oxygen consumption (QO(2)) studies were conducted in adult control, Bcl-xL, lead, and Bcl-xL/lead mice.ResultsThe spherule and pedicle mitochondria in lead-treated mice were swollen, and the cristae structure was markedly changed. In the lead-treated mice, the mitochondrial cristae surface area and volume (abundance: measure correlated with ATP (ATP) synthesis) were decreased in the spherules and increased in the pedicles. Pedicles also had an increased number of crista segments per volume. In the lead-treated mice, the number of segments/crista and fraction of cristae with multiple segments (branching) similarly increased in spherule and pedicle mitochondria. Lead-induced remodeling of spherule mitochondria produced smaller cristae with more branching, whereas pedicle mitochondria had larger cristae with more branching and increased crista junction (CJ) diameter. Lead decreased dark- and light-adapted photoreceptor and dark-adapted photoreceptor synaptic terminal QO(2). Bcl-xL partially blocked many of the lead-induced alterations relative to controls. However, spherules still had partially decreased abundance, whereas pedicles still had increased branching, increased crista segments per volume, and increased crista junction diameter. Moreover, photoreceptor and synaptic QO(2) were only partially recovered.ConclusionsThese findings reveal cellular and compartmental specific differences in the structure and vulnerability of rod and cone inner segment and synaptic mitochondria to postnatal lead exposure. Spherule and pedicle mitochondria in lead-exposed mice displayed complex and distinguishing patterns of cristae and matrix damage and remodeling consistent with studies showing that synaptic mitochondria are more sensitive to Ca(2+) overload, oxidative stress, and ATP loss than non-synaptic mitochondria. The lead-induced decreases in QO(2) likely resulted from the decreased spherule cristae abundance and smaller cristae, perhaps due to Bax-mediated effects as they occurred in apoptotic rod inner segments. The increase in pedicle cristae abundance and CJ diameter could have resulted from increased Drp1-mediated fission, as small mitochondrial fragments were observed. The mechanisms of Bcl-xL-mediated remodeling might occur via interaction with formation of CJ protein 1 (Fcj1), whereas the partial protection of synaptic QO(2) might result from the enhanced efficiency of energy metabolism via Bcl-xL's direct interaction with the F1F0 ATP synthase and/or regulation of cellular redox status. These lead-induced alterations in photoreceptor synaptic terminal mitochondria likely underlie the persistent scotopic and mesopic deficits in lead-exposed children, workers, and experimental animals. Our findings stress the clinical and scientific importance of examining synaptic dysfunction following injury or disease during development, and developing therapeutic treatments that prevent synaptic degeneration in retinal and neurodegenerative disorders even when apoptosis is blocked
Shellfish Spotlight: 2008
Each year Granite State shellfishers search shallow briny waters in search of delicious mussels, clams, or oysters for the dinner table. Those who are skilled often are rewarded with full buckets, but few shellfishers realize that good harvests in New Hampshireâs Seacoast owe much to activities occurring far upstream.
The quality of the water and amount of available nutrients that sustain a clam or
oyster is directly related to the condition of the rivers and streams that drain the land. The Hampton-Seabrook Estuary is fed by approximately 46 square miles of surrounding land. An even larger system, the Piscataqua River Estuary that includes Great Bay, is supplied by a watershed that is 1,023 square miles.
Development within the coastal watershed area has profound impacts on the amount of contaminants flowing to the sea. Sediment washed from roadways and bare soil flows downstream and collects in the estuary where it smothers shellfish beds in
extreme cases. Nutrients, primarily nitrogen, are contributed by wastewater treatment plants, septic systems, and land use activities such as lawn fertilizing. Excessive nutrients threaten the ecological balance of the estuaries and thus the survival of shellfish populations. Finally, bacteria from failing septic systems, pet waste, or damaged sewer systems create a human health hazard in estuarine waters.
Because shellfish filter great amounts of water to take in food and oxygen, they absorb contaminants from the water that accumulate in their flesh. Therefore, a watershed that flushes large amounts of contaminants downstream will deliver many of these contaminants to shellfish and reduce their numbers or often make them unsafe to eat.
It is this close relationship between coastal watershed function and shellfish health that caused the New Hampshire Estuaries Project (NHEP), and many partnering agencies, to monitor shellfish in New Hampshire and make their restoration and maintenance a priority. The NHEP Manage- ment Plan includes many strategies that improve water quality throughout the watershed that will in turn improve shellfish populations and open more harvesting areas
The Evolution of Early-type Field Galaxies Selected from a NICMOS Map of the Hubble Deep Field North
The redshift distribution of well-defined samples of distant early-type
galaxies offers a means to test the predictions of monolithic and hierarchical
galaxy formation scenarios. NICMOS maps of the entire Hubble Deep Field North
in the F110W and F160W filters, when combined with the available WFPC2 data,
allow us to calculate photometric redshifts and determine the morphological
appearance of galaxies at rest-frame optical wavelengths out to z ~ 2.5. Here
we report results for two subsamples of early-type galaxies, defined primarily
by their morphologies in the F160W band, which were selected from the NICMOS
data down to H160_{AB} < 24.0. The observed redshift distributions of our two
early-type samples do not match that predicted by a monolithic collapse model,
which shows an overabundance at z > 1.5. A hierarchical formation model better
matches the redshift distribution of the HDF-N early-types at z > 1.5, but
still does not adequately describe the observed early-types. The hierarchical
model predicts significantly bluer colors on average than the observed
early-type colors, and underpredicts the observed number of early-types at z <
1. [abridged]Comment: Accepted for publication in the Astronomical Journal; 54 pages, 21
figures. Figures 10 and 11 are included separately in JPEG forma
Evolution of brown dwarf disks: A Spitzer survey in Upper Scorpius
We have carried out a Spitzer survey for brown dwarf (BD) disks in the ~5 Myr
old Upper Scorpius (UpSco) star forming region, using IRS spectroscopy from 8
to 12\mu m and MIPS photometry at 24\mu m. Our sample consists of 35 confirmed
very low mass members of UpSco. Thirteen objects in this sample show clear
excess flux at 24\mu m, explained by dust emission from a circum-sub-stellar
disk. Objects without excess emission either have no disks at all or disks with
inner opacity holes of at least ~5 AU radii. Our disk frequency of 37\pm 9% is
higher than what has been derived previously for K0-M5 stars in the same region
(on a 1.8 sigma confidence level), suggesting a mass-dependent disk lifetime in
UpSco. The clear distinction between objects with and without disks as well as
the lack of transition objects shows that disk dissipation inside 5 AU occurs
rapidly, probably on timescales of <~10^5 years. For the objects with disks,
most SEDs are uniformly flat with flux levels of a few mJy, well modeled as
emission from dusty disks affected by dust settling to the midplane, which also
provides indirect evidence for grain growth. The silicate feature around 10\mu
m is either absent or weak in our SEDs, arguing for a lack of hot, small dust
grains. Compared with younger objects in Taurus, BD disks in UpSco show less
flaring. Taken together, these results clearly demonstrate that we see disks in
an advanced evolutionary state: Dust settling and grain growth are ubiquituous
in circum-sub-stellar disks at ages of 5 Myr, arguing for planet forming
processes in BD disks. For almost all our targets, results from high-resolution
spectroscopy and high-spatial resolution imaging have been published before,
thus providing a large sample of BDs for which information about disks,
accretion, and binarity is available. (abridged)Comment: 39 pages, 7 figures, accepted for publication in Ap
A review of how we assess denitrification in oyster habitats and proposed guidelines for future studies
Excess nitrogen (N) loading and resulting eutrophication plague coastal ecosystems globally. Much work is being done to remove N before it enters coastal receiving waters, yet these efforts are not enough. Novel techniques to remove N from within the coastal ecosystem are now being explored. One of these techniques involves using oysters and their habitats to remove N via denitrification. There is substantial interest in incorporating oyster-mediated enhancement of benthic denitrification into N management plans and trading schemes. Measuring denitrification, however, is expensive and time consuming. For large-scale adoption of oyster-mediated denitrification into nutrient management plans, we need an accurate model that can be applied across ecosystems. Despite significant effort to measure and report rates of denitrification in oyster habitats, we are unable to create such a model, due to methodological differences between studies, incomplete data reporting, and inconsistent measurements of environmental variables that may be used to predict denitrification. To make a model that can predict denitrification in oyster habitats a reality, a common sampling and reporting scheme is needed across studies. Here, we provide relevant background on how oysters may stimulate denitrification, and the importance of oyster-mediated denitrification in remediating excess N loading to coastal systems. We then summarize methods commonly used to measure denitrification in oyster habitats, discuss the importance of various environmental variables that may be useful for predicting denitrification, and present a set of guidelines for measuring denitrification in oyster habitats, allowing development of models to support incorporation of oyster-mediated denitrification into future policy decisions
Morphologies and Spectral Energy Distributions of Extremely Red Galaxies in the GOODS-South Field
Using U'- through Ks-band imaging data in the GOODS-South field, we construct
a large, complete sample of 275 ``extremely red objects'' (EROs; K_s<22.0,
R-K_s>3.35; AB), all with deep HST/ACS imaging in B_435, V_606, i_775, and
z_850, and well-calibrated photometric redshifts. Quantitative concentration
and asymmetry measurements fail to separate EROs into distinct morphological
classes. We therefore visually classify the morphologies of all EROs into four
broad types: ``Early'' (elliptical-like), ``Late'' (disk galaxies),
``Irregular'' and ``Other'' (chain galaxies and low surface brightness
galaxies), and calculate their relative fractions and comoving space densities.
For a broad range of limiting magnitudes and color thresholds, the relative
number of early-type EROs is approximately constant at 33-44%, and the comoving
space densities of Early- and Late-type EROs are comparable. Mean rest-frame
spectral energy distributions (SEDs) at wavelengths between 0.1 and 1.2 um are
constructed for all EROs. The SEDs are extremely similar in their range of
shapes, independent of morphological type. The implication is that any
differences between the broad-band SEDs of Early-type EROs and the other types
are relatively subtle, and there is no robust way of photometrically
distinguishing between different morphological types with usual
optical/near-infrared photometry.Comment: Submitted to the ApJL. A version with full-resolution figures, all
GOODS data and all GOODS collaboration papers may be found at
http://www.stsci.edu/science/goods
Preliminary Report of the 2000, 2004, and 2005 Seasons at Tall Jalul, Jordan (Madaba Plains Project)
Since the last full season of excavation in 1999, the Institute of Archaeology at Andrews Univeresity has undertaken three additional expeditions to Tall Jalul as part of the Madaba Plains Project. The first expedition was a brief two-week season, undertaken in 2000, in which a new field of four squares, designated as Field E, was opened immediately north of Field B on the east side of the tall
Sulfur Mustard Induces Markers of Terminal Differentiation and Apoptosis in Keratinocytes Via a Ca2+-Calmodulin and Caspase-Dependent Pathway
Sulfur mustard (SM) induces vesication via poorly understood pathways. The blisters that are formed result primarily from the detachment of the epidermis from the dermis at the level of the basement membrane. In addition, there is toxicity to the basal cells, although no careful study has been performed to determine the precise mode of cell death biochemically. We describe here two potential mechanisms by which SM causes basal cell death and detachment: namely, induction of terminal differentiation and apoptosis. In the presence of 100 ÎŒM SM, terminal differentiation was rapidly induced in primary human keratinocytes that included the expression of the differentiation-specific markers K1 and K10 and the cross-linking of the cornified envelope precursor protein involucrin. The expression of the attachment protein, fibronectin, was also reduced in a time- and dose-dependent fashion. Features common to both differentiation and apoptosis were also induced in 100 ÎŒM SM, including the rapid induction of p53 and the reduction of Bcl-2. At higher concentrations of SM (i.e., 300 ÎŒM), formation of the characteristic nucleosome-sized DNA ladders, TUNEL-positive staining of cells, activation of the cysteine protease caspase-3/apopain, and cleavage of the death substrate poly(ADP-ribose) polymerase, were observed both in vivo and in vitro. Both the differentiation and the apoptotic processes appeared to be calmodulin dependent, because the calmodulin inhibitor W-7 blocked the expression of the differentiation-specific markers, as well as the apoptotic response, in a concentration-dependent fashion. In addition, the intracellular Ca2+ chelator, BAPTA-AM, blocked the differentiation response and attenuated the apoptotic response. These results suggest a strategy for designing inhibitors of SM vesication via the Ca2+-calmodulin or caspase-3/PARP pathway
Radio Observations of the Hubble Deep Field South region: I. Survey Description and Initial Results
This paper is the first of a series describing the results of the Australia
Telescope Hubble Deep Field South (ATHDFS) radio survey. The survey was
conducted at four wavelengths - 20, 11, 6, and 3 cm, over a 4-year period, and
achieves an rms sensitivity of about 10 microJy at each wavelength. We describe
the observations and data reduction processes, and present data on radio
sources close to the centre of the HDF-S. We discuss in detail the properties
of a subset of these sources. The sources include both starburst galaxies and
galaxies powered by an active galactic nucleus, and range in redshift from 0.1
to 2.2. Some of them are characterised by unusually high radio-to-optical
luminosities, presumably caused by dust extinction.Comment: Accepted by AJ. 32 pages, 4 tables, 3 figures. PDF with
full-resolution figures is on
http://www.atnf.csiro.au/people/rnorris/N197.pd
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