Association between monocyte lymphocyte ratio and abdominal aortic calcification in US adults: A cross-sectional study

Background This study aimed to evaluate the association between Monocyte Lymphocyte Ratio (MLR) and Abdominal Aortic Calcification (AAC) in adults over 40 years of age in the United States. Methods Data were collected from the 2013–2014 National Health and Nutrition Examination Survey (NHANES). AAC was quantified by the Kauppila score system based on dual-energy X-Ray absorptiometry. Severe AAC was defined as a total AAC score > 6. The lymphocyte count and monocyte count can be directly obtained from laboratory data files. Multivariable logistic regression models were used to determine the association between MLR and the AAC score and severe AAC. Results A total of 3,045 participants were included in the present study. After adjusting for multiple covariates, MLR was positively associated with higher AAC score (β = 0.21, 95% CI 0.07, 0.34, p = 0.0032) and the odds of severe AAC increased by 14% per 0.1 unit increase in the MLR (OR = 1.14, 95% CI 1.00, 1.31, p = 0.0541). The Odds Ratio (OR) (95% CI) of severe AAC for participants in MLR tertile 3 was 1.88 (1.02, 3.47) compared with those in tertile 1 (p for trend = 0.0341). Subgroup analyses showed that a stronger association was detected in the elderly compared with non-elderly (p for interaction = 0.0346) and diabetes compared with non-diabetes (borderline significant p for interaction = 0.0578). Conclusion In adults in the United States, MLR was associated with higher AAC scores and a higher probability of severe AAC. MLR may become a promising tool to predict the risk of AAC

Seed Heteromorphism: An Important Adaptation of Halophytes for Habitat Heterogeneity

Seed germination is a very critical and important step for seedling establishment under saline environments, as high level of salinity in the soil can prevent seed germination. However halophytes exhibit an interesting mechanism to cope with salt stress. Many halophytes produce heteromorphic seeds, which have different dormancy and germination behavior under saline conditions. This characteristic is related to the structural and physiological differences among heteromorphic seeds. It was unclear that how heteromorphic seeds differently accumulate organic and inorganic substances under saline conditions, and what are the physiological and molecular mechanisms involved in the production of heteromorphic seeds, and in the development of transgenerational plasticity in heteromorphic seeds. In the current brief review, dormancy and germination and the possible role of seed coat and storage compounds in this process of heteromorphic seeds development have been discussed. Moreover, the role of maternal effects on heteromorphic seeds production under saline environments and growth and reproduction capability of the descendants from them have been highlighted

STUDY ON INTELLIGENT MULTI- CONCENTRATES FEEDING SYSTEM FOR DAIRY COW

Abstract: To implement precision feeding for dairy cow, an intelligent multiconcentrates feeding system was developed. The system consists of two parts, one is precision ingredients control subsystem, the other is multi-concentrates discharge subsystem. The former controls the latter with 4 stepper motors. The precision ingredients control subsystem was designed based on Samsung S3C2440 ARM9 microprocessor and WinCE5.0 embedded operating system. The feeding system identifies the dairy cow with passive transponder using RFID (Radio frequency identification) reader. According to the differences of based diet intake and individual dairy cow milk yield, the system can automatically and quantificationally discharge 4 kinds of different concentrates on the basis of the cow identification ID. The intelligent multiconcentrates feeding system for dairy cow has been designed and implemented. According to the experiment results, the concentrate feeding error is less than 5%, the cow inditification delay time is less than 0.5s and the cow inditification error rate is less than 0.01%

KMT2A promotes melanoma cell growth by targeting hTERT signaling pathway.

Melanoma is an aggressive cutaneous malignancy, illuminating the exact mechanisms and finding novel therapeutic targets are urgently needed. In this study, we identified KMT2A as a potential target, which promoted the growth of human melanoma cells. KMT2A knockdown significantly inhibited cell viability and cell migration and induced apoptosis, whereas KMT2A overexpression effectively promoted cell proliferation in various melanoma cell lines. Further study showed that KMT2A regulated melanoma cell growth by targeting the hTERT-dependent signal pathway. Knockdown of KMT2A markedly inhibited the promoter activity and expression of hTERT, and hTERT overexpression rescued the viability inhibition caused by KMT2A knockdown. Moreover, KMT2A knockdown suppressed tumorsphere formation and the expression of cancer stem cell markers, which was also reversed by hTERT overexpression. In addition, the results from a xenograft mouse model confirmed that KMT2A promoted melanoma growth via hTERT signaling. Finally, analyses of clinical samples demonstrated that the expression of KMT2A and hTERT were positively correlated in melanoma tumor tissues, and KMT2A high expression predicted poor prognosis in melanoma patients. Collectively, our results indicate that KMT2A promotes melanoma growth by activating the hTERT signaling, suggesting that the KMT2A/hTERT signaling pathway may be a potential therapeutic target for melanoma

Regulation of the Optical Properties of Cellulose Nanocrystal Films by Sealed Deposition Treatment

Cellulose nanocrystal (CNC) can self-assemble and arrange at specific concentrations, imparting unique optical properties to the system. This paper investigated the effects of sealed deposition time and CNC concentration on the formation and alignment of cholesteric liquid crystals within the naturally dried films by analyzing the changes of CNC films in macroscopic color, UV-vis spectra, polarization optics, microscopic morphology, and crystal structure to elucidate the mechanism of CNC self-assembly behavior during the formation of CNC films. The results showed that when the sealed deposition time was extended to 48 h, the structural color development range and the long-range ordering of the cholesteric phase structure of CNC films were considerably enhanced. As the concentration increased, the CNC particle spacing decreased, the torsion angle between neighboring particles increased, and the pitch was compressed, decreasing from 480 nm to 344 nm. The results of polarized light microscopy analysis demonstrated that the sealed deposition treatment had a significant advantage in the formation of long-range ordered cholesteric phase structure in high-concentration CNC suspensions. The results of this research indicated that prolonging the sealed deposition time and increasing the CNC concentration could enhance the improvement of long-range orderliness in the films and promote the formation of cholesteric phase structural domains. This further improved the scientific basis for the preparation of CNC-based smart packaging materials and had a positive effect on the development of new visual food packaging and inspection materials

The honeysuckle genome provides insight into the molecular mechanism of carotenoid metabolism underlying dynamic flower coloration

Lonicera japonica is a wide-spread member of the Caprifoliaceae (honeysuckle) family utilized in traditional medical practices. This twining vine honeysuckle is also a much-sought ornamental, in part due to its dynamic flower coloration, which changes from white to gold during development. The molecular mechanism underlying dynamic flower coloration in L. japonica was elucidated by integrating whole genome sequencing, transcriptomic analysis, and biochemical assays. Here, we report a chromosome-level genome assembly of L. japonica, comprising nine pseudo-chromosomes with a total size of 843.2 Mb. We also provide evidence for a whole genome duplication event in the lineage leading to L. japonica, which occurred after its divergence from Dipsacales and Asterales. Moreover, gene expression analysis not only revealed correlated expression of the relevant biosynthetic genes with carotenoid accumulation, but also suggested a role for carotenoid degradation in L. japonica's dynamic flower coloration. The variation of flower color is consistent with not only the observed carotenoid accumulation pattern, but also with the release of volatile apocarotenoids that presumably serve as pollinator attractants. Beyond novel insights into the evolution and dynamics of flower coloration, the high-quality L. japonica genome sequence also provides a foundation for molecular breeding to improve desired characteristics

Accelerating complex modeling workflows in CyberWater using on-demand HPC/Cloud resources

Workflow management systems (WMSs) are commonly used to organize/automate sequences of tasks as workflows to accelerate scientific discoveries. During complex workflow modeling, a local interactive workflow environment is desirable, as users usually rely on their rich, local environments for fast prototyping and refinements before they consider using more powerful computing resources. However, existing WMSs do not simultaneously support local interactive workflow environments and HPC resources. In this paper, we present an on-demand access mechanism to remote HPC resources from desktop/laptop-based workflow management software to compose, monitor and analyze scientific workflows in the CyberWater project. Cyber-Water is an open-data and open-modeling software framework for environmental and water communities. In this work, we extend the open-model, open-data design of CyberWater with on-demand HPC accessing capacity. In particular, we design and implement the LaunchAgent library, which can be integrated into the local desktop environment to allow on-demand usage of remote resources for hydrology-related workflows. LaunchAgent manages authentication to remote resources, prepares the computationally-intensive or data-intensive tasks as batch jobs, submits jobs to remote resources, and monitors the quality of services for the users. LaunchAgent interacts seamlessly with other existing components in CyberWater, which is now able to provide advantages of both feature-rich desktop software experience and increased computation power through on-demand HPC/Cloud usage. In our evaluations, we demonstrate how a hydrology workflow that consists of both local and remote tasks can be constructed and show that the added on-demand HPC/Cloud usage helps speeding up hydrology workflows while allowing intuitive workflow configurations and execution using a desktop graphical user interface

Validity and Reliability of the Dyslexia Checklist for Chinese Children

The study on developmental dyslexia (DD) has fairly matured in the past decades, even when there is a lack of a standardized and convenient instrument for dyslexia in the Chinese population. The purpose of this study was to assess the reliability and validity of the Dyslexia Checklist for Chinese Children (DCCC), which was administered to Chinese students in primary school. A total of 545 students from grades 2 through 6 were recruited in Wuhan to participate in this study. We used confirmatory factor analysis (CFA) to evaluate the structure validity of the DCCC. Concurrent validity was determined via correlations between the DCCC and the verbal comprehension index (VCI), and Chinese achievement. The reliability of the DCCC was assessed via test-retest reliability and internal consistency. The CFA suggested that the first order model with eight factors and 55 items fit the data well (RMSEA = 0.057, CFI = 0.930, and TLI = 0.925). The DCCC was negatively associated with VCI (r = −0.218) and Chinese achievement (r = −0.372). The test-retest reliability of the DCCC was 0.734, and the internal consistency of all subscales was above 0.752. The DCCC thus proved to have adequate validity and reliability to screen Chinese dyslexia among students in grades 2 through 6

The honeysuckle genome provides insight into the molecular mechanism of carotenoid metabolism underlying dynamic flower coloration

Lonicera japonica is a wide-spread member of the Caprifoliaceae (honeysuckle) family utilized in traditional medical practices. This twining vine honeysuckle is also a much-sought ornamental, in part due to its dynamic flower coloration, which changes from white to gold during development. The molecular mechanism underlying dynamic flower coloration in L. japonica was elucidated by integrating whole genome sequencing, transcriptomic analysis, and biochemical assays. Here, we report a chromosome-level genome assembly of L. japonica, comprising nine pseudochromosomes with a total size of 843.2 Mb. We also provide evidence for a whole genome duplication event in the lineage leading to L. japonica, which occurred after its divergence from Dipsacales and Asterales. Moreover, gene expression analysis not only revealed correlated expression of the relevant biosynthetic genes with carotenoid accumulation, but also suggested a role for carotenoid degradation in L. japonica’s dynamic flower coloration. The variation of flower color is consistent with not only the observed carotenoid accumulation pattern, but also with the release of volatile apocarotenoids that presumably serve as pollinator attractants. Beyond novel insights into the evolution and dynamics of flower coloration, the high-quality L. japonica genome sequence also provides a foundation for molecular breeding to improve desired characteristics

The genome of hibiscus hamabo reveals its adaptation to saline and waterlogged habitat

Hibiscus hamabo is a semi-mangrove species with strong tolerance to salt and waterlogging stress. However, the molecular basis and mechanisms that underlie this strong adaptability to harsh environments remain poorly understood. Here, we assembled a high-quality, chromosome-level genome of this semi-mangrove plant and analyzed its transcriptome under different stress treatments to reveal regulatory responses and mechanisms. Our analyses suggested that H. hamabo has undergone two recent successive polyploidy events, a whole-genome duplication followed by a whole-genome triplication, resulting in an unusually large gene number (107 309 genes). Comparison of the H. hamabo genome with that of its close relative Hibiscus cannabinus, which has not experienced a recent WGT, indicated that genes associated with high stress resistance have been preferentially preserved in the H. hamabo genome, suggesting an underlying association between polyploidy and stronger stress resistance. Transcriptomic data indicated that genes in the roots and leaves responded differently to stress. In roots, genes that regulate ion channels involved in biosynthetic and metabolic processes responded quickly to adjust the ion concentration and provide metabolic products to protect root cells, whereas no such rapid response was observed from genes in leaves. Using co-expression networks, potential stress resistance genes were identified for use in future functional investigations. The genome sequence, along with several transcriptome datasets, provide insights into genome evolution and the mechanism of salt and waterlogging tolerance in H. hamabo, suggesting the importance of polyploidization for environmental adaptation.DATA AVAILABILITY: The data supporting the findings of this work are available within the paper and its Supporting Information files. The data sets generated and analyzed during this study are available from the corresponding author upon request. All the whole-genome raw data generated during this study have been deposited in the SRA database under BioProject number PRJNA759075. Transcriptome clean data have been deposited in the SRA database under BioProject number PRJNA759717. The final chromosome-scale genome assembly and annotation data have been deposited in the Figshare database (https://doi.org/10.6084/m9.figshare.19142558.v1).Six Talent Peaks Project of Jiangsu Province (NY-042); Open Fund of the Jiangsu Key Laboratory for the Research and Utilization of Plant Resources (JSPKLB201928); Talent Training Funds of the Institute of Botany, Jiangsu Province and Chinese Academy of Sciences.https://academic.oup.com/hrBiochemistryGeneticsMicrobiology and Plant Patholog