Preventive Steps for Pastoral Sexual Misconduct: A Seminar for Brazilian Pastors in the North American Division

Problem In recent years sexual misconduct among clergyman has received an unprecedented public profile as victims have become increasingly willing to speak up and the media has disseminated the stories of clergy abuse. An increasing number of people have been willing to risk their personal reputation and go public with their stories. Effective ministry occurs only within a climate of trust and credibility; however, the ministry is increasingly losing its credibility in society. The goal of this study was to prepare, present, and evaluate a seminar for Brazilian pastors in the North American Division, discussing the main reasons for a pastor’s fall into sexual misconduct and the strategies to keep him from falling. Method This seminar deals with clergy sexual misconduct involving male pastors and female parishioners. This narrow focus does not exhaust the variety of misconduct currently present within the church, although all available statistics indicate that in the majority of cases males tend to be the perpetrators. The seminar was presented May 22-24, 2002, during the Brazilian pastors’ meeting at Pine Springs Ranch, California. This seminar was attended by twenty-seven pastors and their wives. Results All pastors were shocked by the new understanding of this subject. They realized that people are twice as likely to be sexually exploited by the clergy than by secular therapists, and that between 20 and 39 percent of surveyed clergy reported sexual contact with parishioners. They strongly agreed that pastors must be very careful to avoid using pastoral power to take advantage of parishioners and that it is the pastor’s responsibility to be alert to the likelihood that certain parishioners will be attracted to him. He must manage such situations in ways to protect the parishioners. They also recognized that extraordinary safeguards should be kept in place if pastors ever have to practice long-term, cross-gender counseling. Finally, the pastors recognized how important it is for every Seventh-day Adventist pastor to conform to the highest standards of moral conduct as a symbol of the Christian faith for people in the church and in the community at large. Conclusions In the light of responses from Seminar participants and in view of the findings in the literature, the following conclusions have been drawn. Colleges and seminaries should provide a course in ministerial ethics that includes a clear focus for understanding the dynamics of sexual misconduct. The church must be more proactive in addressing the issues of clergy sexual misconduct because the ministerial role places a pastor in a sexually vulnerable position. The pastor who does not understand his vulnerability is either naive or consciously courting danger. Finally, the church leaders should make efforts to train and prepare godly women to counsel women, as is advised in Ellen G. White’s writings

A study of thermodynamic properties using molecular dynamics

Treball de Final de Grau en Química. Codi: QU0943. Curs acadèmic: 2022-202

Leiomiomatosis endovenosa: Abordaje anestésico a propósito de un caso clínico

ResumenJustificativa y objetivosDescribimos aquí el abordaje anestésico a propósito de un caso clínico de leiomiomatosis endovenosa, con invasión de la vena cava inferior y extensión a la aurícula derecha, que tuvo éxito por medio de un abordaje quirúrgico.Relato de casoMujer enferma, de 45 años, aparentemente estable hasta dos semanas antes de su llegada a urgencias, en que debutó con cansancio y disnea. Hizo el ecocardiograma en que se comprobó masa intracardiaca. Por eso, se procedió a la tumorectomía electiva en la transición vena cava-aurícula derecha y ventrículo derecho. En la histología, la pieza operatoria mostró una sugestión de leiomioma uterino. Posteriormente, para evaluar la extensión, se realizó la tomografía computadorizada, que arrojó una extensión a la vena cava inferior y al ovario izquierdo. Entonces se propuso la histerectomía total, anexetomía izquierda y salpingectomía derecha, retirada de leiomioma intravenoso y colocación de filtro definitivo en la vena cava inferior. En este artículo, describimos el abordaje anestésico dándole un énfasis particular a la corrección de las necesidades hídricas, como también a la evolución en el posoperatorio, destacando posibles aportes al abordaje futuro de casos parecidos.ConclusionesEl diagnóstico preoperatorio de leiomiomatosis endovenosa es extremadamente difícil. El tratamiento consiste en la extracción quirúrgica. Ese procedimiento involucra importantes pérdidas hemáticas y por eso, condicionó la preparación preoperatoria. En el intraoperatorio la fluidoterapia fue fundamental y la monitorización invasiva se asumió como preponderante en el auxilio del equilibrio hidroelectrolítico del enfermo. La existencia de un laboratorio de análisis clínicos accesible y con una respuesta rápida y una evaluación de la gasometría fue determinante. La unidad de cuidados intensivos equipada con ventilador para los cuidados postoperatorios fue otra necesidad originada por este caso

Production and Purification of pre-miRNA-149 with potential application in diagnosis of prostate cancer

A few years ago, was discovered that the lin-4 gene did not encode a protein, but a small RNA, responsible for regulating the expression of the LIN-14 protein in Caenorhabditis elegans. This promotes the study of a new class of biomolecules, the non-coding RNAs (ncRNAs), which include the microRNAs (miRNAs). Among them, the miRNA-149 is particularly relevant in the several cellular processes, including oncogenic regulators. Another particularity of this molecule is that the precursor (pre-miRNA-149) have a G-rich sequence, which forms a special nucleic acid secondary structure called G-quadruplex (G4) with a parallel topology that allows its recognition by nucleolin (an important nucleolar protein). This structure has been associated to the regulation of many cellular processes, such as, telomere maintenance, replication, transcription and translation. The present work describes the production and purification of pre-miRNA-149 in E. coli DH5a. For that purpose, a pre-miRNA-149 DNA sequence was cloned into a pBHSR1-RM plasmid and inserted within the previous competent cells by thermic shock. After fermentation, total RNA was and purified by affinity chromatography taking advantage of the biological recognition of pre-miRNA-149. Three affinity supports are used such as naphthalene amine Sepharose, Ltyrosine Sepharose and L-lysine Sepharose. Each of them has already been successfully used to purify similar biomolecules. The eluted fractions are evaluated by denaturing PAGE. The complete isolation of pre-miRNA-149 was not achieved in the three supports tested; however, some promising results were obtained with the L-lysine support, where a small portion of pre-miRNA-149 was isolated into one single fraction (low recovery rate), with a decreasing stepwise gradient from 2.05 M to 0.1 M of (NH4)2SO4 in Tris 10 mM (pH 6.0). Future affinity chromatography experiments are required to optimize the conditions to separate the pre-miRNA-149 from the total RNA mixture, to successfully use miRNA-based therapies.Há alguns anos, descobriu-se que o gene lin-4 não codificava uma proteína, mas um pequeno RNA, responsável por regular a expressão da proteína LIN-14 em Caenorhabditis elegans. Deste modo, uma nova classe de biomoléculas emerge, os RNAs não-codificantes (ncRNAs), que incluem os microRNAs (miRNAs). Entre eles, o miRNA-149 é particularmente relevante em diversos processos celulares, incluindo os reguladores oncogénicos. Outra particularidade desta molécula é que o seu precursor (pre-miRNA-149) possui uma sequência rica em nucleótidos de guanina, que forma uma estrutura secundária denominada de G-quadruplex (G4), com topologia paralela que permite o seu reconhecimento pela proteína nucleolina. A estrutura de G4 tem sido associada à regulação de muitos processos celulares, como manutenção, replicação, transcrição e tradução de telómeros. O presente trabalho descreve a produção e purificação de pre-miRNA-149 em E. coli DH5a. Para esse propósito, uma sequência de DNA pre-miRNA-149 foi clonada em um plasmídeo pBHSR1-RM e inserida nas células competentes por choque térmico. Após a fermentação, o RNA total foi purificado por cromatografia de afinidade, aproveitando o reconhecimento biológico do pre-miRNA-149. Utilizam-se três suportes cromatográficos de afinidade que foram naftaleno amina Sepharose, L-tirosina Sepharose e L-lisina Sepharose. Cada um deles já foi usado com sucesso para purificar biomoléculas similares. As frações eluídas foram avaliadas por PAGE desnaturante. O isolamento completo do pre-miRNA-149 não foi alcançado em nenhum dos suportes; no entanto, foram obtidos alguns resultados promissores com o suporte de L-lisina, onde uma pequena porção de pre-miRNA-149 foi isolada num passo único, com gradiente decrescente de 2,05 M a 0,1 M de (NH4)2SO4 em Tris 10 mM (pH 6,0). Como trabalho futuro pretende-se otimizar as condições cromatográficas experimentais que permitam separar o pre-miRNA-149 da mistura de RNA total, para ser utilizado em terapias baseadas em miRNA

Estratificação geográfica por nível sócio-econômico: metodologia utilizada em um inquérito domiciliar com idosos residentes em São Paulo, Brasil

Considering that in most developing countries there are still no comprehensive lists of addresses for a given geographical area, there has always been a problem in drawing samples from the community, ensuring randomisation in the selection of the subjects. This article discusses the geographical stratification by socio-economic status used to draw a multistage random sample from a community-based elderly population living in a city like S. Paulo - Brazil. Particular attention is given to the fact that the proportion of elderly people in the total population of a certain area appeared to be a good discriminatory variable for such stratification. The validity of the stratification method is analysed in the light of the socio-economic results obtained in the survey.Em países como o Brasil é sempre problemático conseguir-se uma amostra populacional randomisada e representativa dada a inexistência de um catálogo atualizado e global da população residente na comunidade. Discute-se a metodologia de estratificação geográfica por nível sócio-econômico utilizada para conseguir uma amostra randomisada em múltiplos estágios de uma população de idosos residentes na cidade de São Paulo. O estudo aponta para o fato de a proporção de idosos na população total de uma determinada área da cidade ter se mostrado uma variável com poder discriminante do nível sócio-econômico da população. Discute-se a validade do método de estratificação a partir dos dados coletados no inquérito

STUDI KELAYAKAN IMPLEMENTASI SAP R/3 MODUL MATERIAL MANAGEMENT (STUDI KASUS : SAP ECC 6.0 PADA BADAN OPERASI BERSAMA PT. BSP - PERTAMINA HULU)

STUDI KELAYAKAN IMPLEMENTASI SAP R/3 MODUL MATERIAL MANAGEMENT (STUDI KASUS : SAP ECC 6.0 PADA BADAN OPERASI BERSAMA PT. BSP - PERTAMINA HULU) - Studi Kelayakan, Material Management, SAP R/3, SAP ECC 6.

Diseño de la ampliación de la red del alcantarillado del Asentamiento Humano El Paraiso del distrito de la Arena - Piura

El presente trabajo de investigación tiene por objetivo diseñar la red del alcantarillado sanitario del A.A.H.H El Paraíso ya que hasta el momento no cuenta con este servicio básico, haciendo sus necesidades en acequias, y canales de tierras, contaminando así dichos lugares. En el desarrollo del diseño de la Red del Alcantarillado Sanitario se recaudó información insitu, completándolo con la información obtenido de la Municipalidad Distrital de la Arena. Se realizo estudios poblacionales determinándose una población beneficiaria de 505 habitantes que ocupan 133 viviendas, para un periodo de diseño de 20 años siendo este diseño hasta el año 2041. Para el diseño de la red del alcantarillado sanitario se usó el software SewerGEMS, en la caudal obtuvimos los datos hidráulicos siendo el caudal de diseño de 1.5 l/s en la cual ese cual descargaría a la cámara de bombeo existente en la zona de estudio, ya que el caudal obtenido de los estudios de la población del asentamiento humano el paraíso es un caudal menor al caudal que recomienda el reglamento nacional de edificación siendo el caudal de la población de 0.945 l/s , se optó por diseñar con el caudal que recomienda el reglamento que es de 1.5 l/s. El diseño de la red del alcantarillado sanitario cuenta con 20 buzones, en la cual 4 buzones son con caída especial siendo los buzones (Bz-04, Bz-05, Bz-07, Bz-08), contando en el diseño de la red con 20 tramos de tubería de colector de PVC SERIE 25 SN 2/ SDR 51 de diámetro nominal 160 mm siendo un total de 38.54ml, también con tubería de conexiones domiciliarias de PVC SERIE 25 SN 2/ SDR 51 de diámetro nominal 110 mm teniendo un total de 302.65 ml.The objective of this research work is to design the sanitary sewer network of the A.A.H.H El Paraíso since until now it does not have this basic service, making its needs in ditches and land channels, thus contaminating these places. In the development of the design of the Sanitary Sewer Network, information was collected on-site, completing it with the information obtained from the District Municipality of La Arena. Population studies were carried out, determining a beneficiary population of 505 inhabitants who occupy 133 homes, for a design period of 20 years, this design being until the year 2041. For the design of the sanitary sewer network, the Sewer GEMS software was used, in the flow we obtained the hydraulic data being the design flow of 1.5 l / s in which that which would discharge to the existing pumping chamber in the study area, Since the flow obtained from the studies of the population of the human settlement El Paraíso is a flow lower than the flow recommended by the national building regulations, the population flow being 0.945 l / s, it was decided to design with the flow recommended the regulation that is 1.5 l / s. The design of the sanitary sewer network has 20 mailboxes, in which 4 mailboxes have a special drop, being the mailboxes (Bz-04, Bz-05, Bz-07, Bz-08), counting in the design of the network with 20 sections of PVC collector pipe SERIES 25 SN 2 / SDR 51 with a nominal diameter of 160 mm, for a total of 38.54ml, also with PVC domestic connection pipe SERIES 25 SN 2 / SDR 51 with a nominal diameter of 110 mm having a total of 302.65 ml.Tesi

Urban growth in the long term: Belgium, 1880–1970

We take advantage of a new data set on Belgian cities to test random growth, that is, Gibrat’s law. This unique data set provides annual population estimates for all Belgian municipalities (2680 cities) from 1880 to 1970. The use of panel data methodology and unit root tests can provide a precise test of Gibrat’s law (a unit root is equivalent to random growth). We run both time series and panel data unit root tests, thus obtaining strong support for random growth in the long term. Results hold when allowing for the presence of one and two structural breaks in the mean, with the timing of the breaks coinciding with some major historical events, such as the World Wars and the economic crisis of 1929–1933

Design and development of a multiscale model for the osteoporotic fracture prevention: a preclinical tool

Se espera que la osteoporosis sea partícipe de más de 9 millones de nuevas fracturas en todo el mundo en un futuro no muy lejano, ya que es una de las enfermedades con mayor índice de impacto entre la población de los países desarrollados. Se define como una enfermedad sistémica caracterizada por la pérdida de masa ósea y una alteración de su microestructura interna con la consiguiente susceptibilidad a la fractura. Actualmente, la estimación del riesgo de fractura se lleva a cabo mediante tomografía axial computerizada (TAC), Rayos X o densitometrías. Sin embargo, las simulaciones por elementos finitos para un paciente determinado, pueden contener una gran cantidad de información que permitirían unas predicciones más precisas. Una metodología multiescala ayudaría al desarrollo y caracterización de modelos de fractura más robustos que permitirían conocer de una manera más detallada el comportamiento del hueso. Además, dichos modelos podrían incorporar parámetros relacionados con la edad, el grado de osteoporosis o el tratamiento mediante fármacos. De hecho, debido a que el hueso trabecular interviene, en gran medida, en las fracturas de cadera osteoporóticas, un tratamiento preventivo alternativo para reducir el riesgo de fractura osteoporótica consistiría en la inyección de cemento óseo (PMMA) en el fémur osteoporótico.Por lo tanto, el principal objetivo de esta tesis doctoral es el desarrollo de un modelo multiescala para la prevención de la fractura ósea osteoporótica. Este modelo nos permitirá conocer más acerca de los mecanismos de fallo asociados a la osteoporosis desde el nivel tisular hasta el nivel macroscópico a fin de evaluar la factibilidad de la femoroplastia. Para alcanzar este objetivo, en primer lugar, se ha llevado a cabo una caracterización in vitro e in silico de estructuras artificiales de hueso artificial, denominadas open-cell (Sawbones, Malmö, Sweden), con propiedades próximas al hueso sano y osteoporótico, de manera que permita elucidar mecanismos de fractura asociados a la osteoporosis desde el nivel tisular. De esta manera, se han empleado métodos experimentales y computacionales basados en el procesado de imagen con el fin de estimar el módulo elástico y las porosidades de las diferentes estructuras open-cell. Las resultados computacionales y experimentales fueron comparados con los datos aportados por el fabricante. Se apreciaron importantes diferencias no sólo en términos del módulo de Young sino también en las porosidades. Posteriormente, se desarrolló un modelo discreto de partículas basado en la Teoría del Movimiento Aleatorio para simular la infiltración de cemento a través de las estructuras open-cell, previamente caracterizadas. Los parámetros del modelo incluyeron no sólo la viscosidad del cemento (alta o baja) sino la dirección de inyección (vertical o diagonal). De nuevo, se llevó a cabo una caracterización in vitro e in silico de las estructuras cementadas, validando el modelo computacional mediante ensayos experimentales. Dichos resultados mostraron que el modelo discreto de partículas era suficientemente robusto para su aplicación en la escala macroscópica. También, se inyectó cemento in vivo en fémures de conejo a fin de evaluar la factibilidad de la femoroplastia. Finalmente, se utilizaron fémures sanos y osteoporóticos para la predicción computacional del grado de mejora de las propiedades mecánicas cuando se inyectaba cemento de alta o baja viscosidad. El cemento de baja viscosidad mejoraba notablemente las cargas de fractura con respecto a los fémures no cementados. Los resultados finales mostraron que el cemento óseo mejora definitivamente las propiedades del hueso osteoporótico y la metodología propuesta puede llegar a utilizarse como una herramienta preclínica para un diagnóstico más preciso.<br /

Improving Emergency Response Decisions by Using Web 2.0

Emergency response organizations such as the American Red Cross are delving into Web 2.0 applications. Although, social media has flattened the communication hierarchy, large organizations continue to use traditional top-down modes of communication. There is an opportunity to use social media to document the events of an emergency as it happens. The knowledge of a crisis could be captured first-hand by the victims of that emergency and then fed up the communication channel in a bottom-up approach through Web 2.0 applications