Features associated to woody hosts in the bacterial pathogen of olive plants Pseudomonas savastanoi pv. savastanoi

The causal agent of olive knot disease, Pseudomonas savastanoi pv. savastanoi, belongs to the Pseudomonas syringae complex, a bacterial group causing diseases in a broad variety of both woody and herbaceous plant species. Here we summarize our results regarding a set of P. savastanoi pv. savastanoi features exclusively found in the genomes of bacteria from the P. syringae complex isolated from woody hosts. Comparative genomics and evolutionary studies allowed us to identify a 15 kb genomic island (WHOP, from woody host and Pseudomonas), carrying a set of genes involved in degradation of phenolic compounds and exclusively found in bacterial pathogens of woody hosts. Deletion of several WHOP-encoded genes in Pseudomonas savastanoi pv. savastanoi NCPPB 3335 revealed that they play a role in the virulence of the strain in woody olive plants but not in in vitro-grown (nonwoody) plants. In addition, several type III secretion system effectors belonging to the HopAF, HopAO and HopBL families were shown to be clustered across the P. syringae complex according to the woody/herbaceous nature of their host of isolation. Further functional analyses of these virulence factors are needed to facilitate the design of novel strategies directed to control bacterial pathogens of woody hosts.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Assessment of the structures contribution (crystalline and mesophases) and mechanical properties of polycaprolactone/pluronic blends

Films of biodegradable blends of polycaprolactone (PCL) and Pluronics F68 and F127 were manufactured by an industrial thermo-mechanical process to be applied as potential delivery systems. The effects of Pluronics on the structure (mesophase organization), and thermal and mechanical properties of polycaprolactone were investigated using differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS), X-ray diffraction (XRD), polarized optical microscopy (POM) and tensile mechanical tests. The addition of Pluronics affected the crystallization process by changing the relative amounts of crystalline, amorphous, and meso- (condis + plastic) phases. The melting transition and XRD profiles were deconvoluted to assess the individual contribution of the different crystal morphologies. Furthermore, it was found that the mechanical properties of the blends depended on the ratio and type of Pluronic. Thus, Pluronic F127 showed a larger mesophase content than its F68 counterpart with PCL and blends with enhanced ductilityFunding for open access charge was provided by Universidad de Huelva / CBUA. The authors gratefully acknowledge the financial support

Primer registro de Achimenes admirabilis (Gesneriaceae) para la flora de Tabasco, México

ResumenSe da a conocer un nuevo registro de Achimenes admirabilis para México. Esta especie solo se había registrado en los estados de Oaxaca y Veracruz. Colectas recientes en el área municipal de Tenosique, extienden el intervalo de distribución de la especie al estado de Tabasco.AbstractA new record of Achimenes admirabilis is reported for Mexico. This species had only been reported in the states of Oaxaca and Veracruz. Recent collections in the municipality of Tenosique, extend its range to the state of Tabasco

Chronodisruption: A poorly recognized feature of CKD

Multiple physiological variables change over time in a predictable and repetitive manner, guided by molecular clocks that respond to external and internal clues and are coordinated by a central clock. The kidney is the site of one of the most active peripheral clocks. Biological rhythms, of which the best known are circadian rhythms, are required for normal physiology of the kidneys and other organs. Chronodisruption refers to the chronic disruption of circadian rhythms leading to disease. While there is evidence that circadian rhythms may be altered in kidney disease and that altered circadian rhythms may accelerate chronic kidney disease (CKD) progression, there is no comprehensive review on chronodisruption and chronodisruptors in CKD and its manifestations. Indeed, the term chronodisruption has been rarely applied to CKD despite chronodisruptors being potential therapeutic targets in CKD patients. We now discuss evidence for chronodisruption in CKD and the impact of chronodisruption on CKD manifestations, identify potential chronodisruptors, some of them uremic toxins, and their therapeutic implications, and discuss current unanswered questions on this topicThis work was funded by FIS CP14/00133, PI16/02057, PI18/01366, PI19/00588, PI19/00815, DTS18/00032, ERA-PerMed-JTC2018 (KIDNEY ATTACK AC18/00064 and PERSTIGAN AC18/00071, National Institute of Health (2R01AI063331), ISCIII-RETIC REDinREN RD016/0009 Fondos FEDER, FRIAT, Sociedad Española de Nefrología, Comunidad de Madrid B2017/BMD-3686 CIFRA2-CM, Miguel Servet MS14/00133 to MDSN and ABS. IIS-Fundacion Jimenez Diaz Biobank, part of the Spanish Biobanks Platform (PT17/0015/0006). The APC was funded by PI19/0081

Clinical proteomics in kidney disease as an exponential technology: Heading towards the disruptive phase

Exponential technologies double in power or processing speed every year, whereas their cost halves. Deception and disruption are two key stages in the development of exponential technologies. Deception occurs when, after initial introduction, technologies are dismissed as irrelevant, while they continue to progress, perhaps not as fast or with so many immediate practical applications as initially thought. Twenty years after the first publications, clinical proteomics is still not available in most hospitals and some clinicians have felt deception at unfulfilled promises. However, there are indications that clinical proteomics may be entering the disruptive phase, where, once refined, technologies disrupt established industries or procedures. In this regard, recent manuscripts in CKJ illustrate how proteomics is entering the clinical realm, with applications ranging from the identification of amyloid proteins in the pathology lab, to a new generation of urinary biomarkers for chronic kidney disease (CKD) assessment and outcome prediction. Indeed, one such panel of urinary peptidomics biomarkers, CKD273, recently received a Food and Drug Administration letter of support, the first ever in the CKD field. In addition, a must-read resource providing information on kidney disease-related proteomics and systems biology databases and how to access and use them in clinical decision-making was also recently published in CKJ.Grant support was received from: ISCIII and FEDER funds PI13/00047; EUTOX, CP12/03262, CP14/00133, PI15/00298, PI14/00386, PI15/01460, PI16/01900, PI16/02057; Diabetes Cancer Connect PIE13/00051; Sociedad Española de Nefrologia; FRIAT; and ISCIII-RETIC REDinREN RD016/009. Salary support was received from: ISCIII Miguel Servet to A.B.S., A.M.R. and M.D.S.-N.; Joan Rodes to B.F.-F; and Programa Intensificación Actividad Investigadora (ISCIII/Agencia Laín-Entralgo/CM) to A.O

Excitons, trions and Rydberg states in monolayer MoS2 revealed by low temperature photocurrent spectroscopy

We investigate excitonic transitions in a h-BN encapsulated monolayer $\textrm{MoS}_2$ phototransistor by photocurrent spectroscopy at cryogenic temperature (T = 5 K). The spectra presents excitonic peaks with linewidths as low as 8 meV, one order of magnitude lower than in earlier photocurrent spectroscopy measurements. We observe four spectral features corresponding to the ground states of neutral excitons ($\textrm{X}_{\textrm{1s}}^\textrm{A}$ and $\textrm{X}_{\textrm{1s}}^\textrm{B}$) and charged trions ($\textrm{T}^\textrm{A}$ and $\textrm{T}^\textrm{B}$) as well as up to eight additional spectral lines at energies above the $\textrm{X}_{\textrm{1s}}^\textrm{B}$ transition, which we attribute to the Rydberg series of excited states of $\textrm{X}^\textrm{A}$ and $\textrm{X}^\textrm{B}$. The relative intensities of the different spectral features can be tuned by the applied gate and drain-source voltages, with trions and Rydberg excited states becoming more prominent at large gate voltages. Using an effective-mass theory for excitons in two-dimensional transition-metal dichalcogenides we are able to accurately fit the measured spectral lines and unambiguously associate them with their corresponding Rydberg states. The fit also allows us to determine the quasiparticle bandgap and spin-orbit splitting of monolayer $\textrm{MoS}_2$, as well as the exciton binding energies of $\textrm{X}^\textrm{A}$ and $\textrm{X}^\textrm{B}$

Effects of a 48-Day Home Quarantine during the Covid-19 Pandemic on the First Outdoor Running Session among Recreational Runners in Spain

[Abstract] COVID-19-induced quarantine may lead to deleterious effects on health status as well as to impaired performance and increased injury risk when re-starting training after lockdown. We investigated the physical activity (PA) habits of recreational runners in Spain during a 48-day home quarantine during the COVID-19 pandemic and the characteristics of the first outdoor running session after confinement. A cross-sectional study, including a self-reported running questionnaire completed after the first outdoor running session after quarantine, was performed. Three hundred recreational runners (74% males; 60% 18–40 years old; most typical running experience >3 years, 10– 30 km weekly running distance distributed in 3–4 sessions) were considered for analysis. Advanced runners ran, at least, 4 days/week and participated in running events. They performed significantly longer and more non-supervised weekly training sessions during confinement (p < 0.01 for both) than novice and amateur runners. Most runners performed their first outdoor running session on asphalt (65.3%) and ran 5 to 10 km (61%) at a pace above 5 min/km (60%), reporting no pain before (77%), during (64%), and 24 h after (76%) the session. Advanced runners performed a significantly longer running session, at a higher pace, and covered a greater distance (p < 0.01 for all) than novice and amateur runners, while enjoyment and motivation tended to be significantly higher when runners’ level increased (p < 0.05). Higher training levels prior to and during confinement may lower the collateral effects (e.g., detraining, injury risk) of home quarantine when runners return to previous PA levels

Behavior of vine varieties resistant to fungal diseases in the somontano region

The vine is highly susceptible to numerous fungal diseases, the incidence and severity of which are increasing because of climate change. To fight them, large amounts of phytosanitary products are generally used, although they entail important economic and environmental costs. The new fungus resistant vine varieties (PIWI) constitute one of the most active lines of research on plant material in viticulture and are regarded as a viable solution to respond to the requirements of European Directive 2009/128/EC. In the present work, the evolution and impact of the three main fungal diseases (powdery mildew, downy mildew and botrytis bunch rot) were monitored in plots in the Somontano region (Huesca, Spain), comparing three PIWI varieties (Sauvignon Kretos, Souvignier gris and Muscaris) with a susceptible control variety (Sauvignon blanc) in real field growing conditions over three successive years (2016–2018). The main ampelographic characters of each variety were studied and a weekly follow-up was carried out to track the development of each disease, relating it to climatic variables. Regardless of the climatic conditions (one of the growing seasons was rainy and the other two corresponded to dry years, with differences in the distribution of rainfall), the three PIWI varieties hardly presented any symptoms, demonstrating a high resistance to downy mildew and powdery mildew and even to botrytis. Principal component analysis results pointed to a differential behavior versus the control and treated Sauvignon blanc plants in terms of disease resistance. Based on production results, despite the youth of the plantation, the excellent potential of Sauvignon Kretos variety was observed. However, from the analysis of the quality of wine obtained in microvinifications from these fungus resistant varieties in the second and third year, the one obtained from Muscaris seemed to have the best organoleptic properties. Apart from environmental considerations, given that these fungus resistant varieties can significantly contribute to the sustainability of wineries, they may also respond to the demand of consumers who want zero-pesticide products or of the supporters of ecological products adjusted to European regulations

Effective nephroprotection against acute kidney injury with a star-shaped polyglutamate-curcuminoid conjugate

The lack of efective pharmacological treatments for acute kidney injury (AKI) remains a signifcant public health problem. Given the involvement of apoptosis and regulated necrosis in the initiation and progression of AKI, the inhibition of cell death may contribute to AKI prevention/recovery. Curcuminoids are a family of plant polyphenols that exhibit attractive biological properties that make them potentially suitable for AKI treatment. Now, in cultured tubular cells, we demonstrated that a crosslinked self-assembled star-shaped polyglutamate (PGA) conjugate of bisdemethoxycurcumin (StPGA-CL-BDMC) inhibits apoptosis and necroptosis induced by Tweak/TNFα/IFNγ alone or concomitant to caspase inhibition. St-PGA-CL-BDMC also reduced NF-κB activation and subsequent gene transcription. In vivo, St-PGA-CL-BDMC prevented renal cell loss and preserved renal function in mice with folic acid-induced AKI. Mechanistically, St-PGA-CL-BDMC inhibited AKI-induced apoptosis and expression of ferroptosis markers and also decreased the kidney expression of genes involved in tubular damage and infammation, while preserving the kidney expression of the protective factor, Klotho. Thus, due to renal accumulation and attractive pharmacological properties, the application of PGAbased therapeutics may improve nephroprotective properties of current AKI treatmentsTis work was supported by grants from the Instituto de Salud Carlos III, FEDER funds: PI16/02057, PI16/01900, PI18/01133, PI19/00815, ISCIII RETIC REDINREN RD16/0009; Sociedad Española de Nefrología; FRIAT; Comunidad de Madrid en Biomedicina B2017/BMD-3686 CIFRA2-CM; ERA-PerMed-JTC2018 (AC18/00071; DTS18/00032); Spanish Ministry of Economy and Competitiveness (Grant numbers SAF2013-44848-R, SAF2016-80427-R). Partly co-funded by FEDER (PO FEDER Valencian Community - 2014–2020