(Anti-)deuteron production and anisotropic flow measured with ALICE at the LHC

The high abundance of (anti-)deuterons in the statistics gathered in Run 1 of the LHC and the excellent performance of the ALICE setup allow for the simultaneous measurement of the elliptic flow and the deuteron production rates with a large transverse momentum ($p_{\rm T}$) reach. The (anti-) deuterons are identified using the specific energy loss in the time projection chamber and the velocity information in the time-of-flight detector. The elliptic flow of (anti-)deuterons can provide insight into the production mechanisms of particles in heavy-ion collisions. Quark coalescence is one of the approaches to describe the elliptic flow of hadrons, while the production of light nuclei can be also depicted as a coalescence of nucleons. In these proceedings, the measured $v_{2}$ of deuterons produced in Pb--Pb collisions at $\sqrt{s_{\mathrm{NN}}}$=2.76TeV will be compared to expectations from coalescence and hydrodynamic models.Comment: 4 pages, 3 figures, proceeding of Quark Matter 2015, Kobe, Japa

Hypernuclei production in Pb–Pb collisions at sNN=2.76 TeV with ALICE at the LHC

AbstractResults on (anti)hypertriton production in Pb–Pb collisions at sNN=2.76 TeV are reported using the data samples collected by the ALICE experiment during the LHC heavy-ion runs at the end of 2011.The (anti)HΛ3 signal is extracted from the study of its mesonic decay (HΛ3→He3+π−) via the topological identification of secondary vertices.The (He3/He¯3,π) invariant mass distributions in different transverse momentum intervals are shown

Light hypernuclei production in Pb-Pb collisions with ALICE at LHC

2011/2012The subject of the present PhD thesis is the study of the production of light hypernuclei in ultra-relativistic Pb-Pb collisions with ALICE (A Large Ion Collider Experiment), one of the four major experiments at the LHC (Large Hadron Collider). The main physics goal of the ALICE experiment is the investigation of the properties of the strongly interacting matter at high energy density ($>$ 10 GeV/fm$^3$) and high temperature ($\approx$ 0.2 GeV) conditions. According to the lattice Quantum Chromo Dynamics (QCD) calculations, under these conditions (i.e. high temperature and large energy density) hadronic matter undergoes a phase transition to a ``plasma'' of deconfined quarks and gluons (Quark Gluon Plasma, QGP). In the first chapter of the thesis a general introduction to the heavy-ion physics will be given. Then the main quantities related to QGP formation (i.e. \textit{probes}) will be described. Finally the most important results obtained at SPS, RHIC and LHC experiments will be shown and discussed. In the second chapter a short description of the LHC and its experimental conditions will be reported and an overview of the ALICE experiment will be given. A description of the different detectors and their performances during data taking will be described; in addition a description of the computing framework will be given. The third chapter will be devoted to an introduction of the (anti)(hyper)nuclei production in heavy-ion collisions. The two main approaches which are believed to govern nuclei production (i.e. coalescence and thermal models) will be described, and an overview on the results at different energies will be shown. A comparison of the theoretical results will be also shown, with particular regards to the energies at the LHC. The fourth chapter is devoted to the description of the analysis method used to get (anti)hypertriton production yield in \PbPb~collisions at $\sqrt{s_{\rm{NN}}}$ = 2.76~TeV with the ALICE experiment via its mesonic decay \hyp~$\rightarrow$ \he + \pim (\antihyp $\rightarrow$ \antihe + \pip). In the beginning of the chapter the analysis technique used for particle identification and for the determination of secondary vertices will be described. The analysis will be divided into two distinct parts: the first one based on the data sample collected by the ALICE experiment during the first LHC heavy-ion run held at the end of 2010, while the second one based on data collected at the end of 2011. A detailed description of the study on efficiency evaluation and signal extraction will be shown for both analysis, together with a study of the systematic uncertainties. The results on the production yield of (anti)hypertriton will also be shown. The estimation of the hypertriton lifetime will be provided in the final section of the chapter.\\ In the fifth chapter the method used to obtain the \pt~spectrum of \he~will be presented. The raw spectra, the efficiency evaluation, systematic errors and feed-down from \hyp~will be presented. The final spectrum will be used to evaluate the production yield of \he(\antihe) in the whole \pt~region, from 0 to $\infty$. \\ Finally, in the last chapter, the present experimental results will be compared with published relevant results and with the most recent theoretical findings. Moreover, the measurement of the ``Strangeness Population Factor'' [S$_{3}$= \hyp/\he/($\Lambda$/p)] at the LHC energies will be provided. This quantity is a valuable tool to probe the nature of dense matter created in high-energy heavy-ion collisions and to validate theoretical models.-------------------------------------------------------------------------------------------------- Questa tesi è dedicata allo studio della produzione di ipernuclei leggeri in collisioni ultra-relativistiche di ioni piombo (Pb) con l'esperimento ALICE (A Large Ion Collider Experiment), uno dei quattro grandi esperimenti del Large Hadron Collider (LHC) del CERN. Il principale obiettivo scientifico dell'esperimenento ALICE è lo studio delle proprietà della materia in condizioni estreme di energia (> 10 GeV/fm^3) e di temperatura (~ 0.2 GeV) mediante lo studio di collisioni di ioni piombo. Calcoli di Cromo Dinamica Quantistica (QCD) su reticolo prevedono, infatti, che in condizioni di alta temparatura e grande energia la materia adronica subisca un transizione di fase verso un ``plasma'' di quark e gluoni deconfinati (Quark Gluon Plasma, QGP). Nel primo capitolo della tesi verranno descritte in maniera generale la fisica degli ioni pesanti e le grandezze caratterische usate per provare la formazione del QGP (probes). Verranno quindi mostrati e discussi i risultati sperimentali che possono provare l'esistenza di uno stato deconfinato della materia nucleare ottenuti agli esperimenti a SPS, RHIC e LHC. Nel secondo capitolo saranno brevemente presentati il Large Hadron Collider (LHC) e le condizioni sperimentali di lavoro durante i primi tre anni di presa dati; in seguito verrà data un'ampia panoramica dell'esperimento ALICE. Saranno descritti i differenti sotto-rivelatori che formano l'esperimento e verranno inoltre mostrate le loro performance durante l'acquisione dati; inoltre verrà fornita una descrizione del framework di calcolo utilizzato nell'analisi dei dati. Il terzo capitolo sarà dedicato alla descrizione dei maccanismi di produzione di (anti)(iper)nuclei in collisioni di ioni pesanti: verranno descritti i due meccanismi di produzione che si ritiene governino la loro produzione (coalescenza e modello termico) e verrà mostrata una panoramica sui risultati ottenuti a diverse energie. Inotre saranno presentati diversi calcoli teorici, ponendo particolare attenzione ai risultati aspettati all'energia di LHC. Il quarto capitolo contiene la descrizione del metodo di analisi utilizzato per valutare lo yield di pruduzione dell'(anti)ipertritone attraverso il suo canale di decadimento mesonico \hyp~$\rightarrow$ \he + \pim (\antihyp $\rightarrow$ \antihe + \pip) in collisioni \PbPb~con energia nel centro di massa $\sqrt{s_{\rm{NN}}}$ = 2.76~TeV. Inizialmente verrà descritta la tecnica di analisi utilizzata per l'identificazione di particelle e dei vertici secondari, quindi sarà fornita la descrizione dettagliata della tecnica di analisi. L'analisi dei dati è stata siddivisa in due distinte parti: la prima è dedicata alla descrizione della procedura utilizzata per l'analisi dei dati raccolti da ALICE durante la prima acquisizione di collisioni Pb--Pb alla fine del 2010; nella seconda parte, invece, verrà descritta la procedura di analisi dei dati raccolti durante la seconda presa dati nel Dicembre 2011. Verranno quindi descritte in modo dettagliato l'estrazione del segnale, lo studio del fondo combinatoriale e gli errori sistematici. Infine, nella parte finale del capitolo, varrà fornita una stima della vita media dell'ipertritone.\\ Nel quinto capitolo sarà presentato il metodo usato per ottenere lo spettro in pT di (anti-3He)3He. Verranno descritti: la procedura di estrazione del segnale, la stima dell'efficienza in funzione del momento trasverso, la valutazione degli errori sistematici e la procedure usata per sottrarre il feed-down dovuto al decadimento dell'ipertitone. Lo spettro verrà quindi utilizzato per valutare lo yield di produzione di (anti-3He) 3He. Infine, nel sesto e ultimo capitolo, i risultati sperimentali ottenuti verranno confrontati con i risultati teorici discussi nel Capitolo 3.XXV Ciclo198

Biological Risk Assessment of Three Dental Composite Materials following Gas Plasma Exposure

Gas plasma is an approved technology that generates a plethora of reactive oxygen species, which are actively applied for chronic wound healing. Its particular antimicrobial action has spurred interest in other medical fields, such as periodontitis in dentistry. Recent work has indicated the possibility of performing gas plasma-mediated biofilm removal on teeth. Teeth frequently contain restoration materials for filling cavities, e.g., resin-based composites. However, it is unknown if such materials are altered upon gas plasma exposure. To this end, we generated a new in-house workflow for three commonly used resin-based composites following gas plasma treatment and incubated the material with human HaCaT keratinocytes in vitro. Cytotoxicity was investigated by metabolic activity analysis, flow cytometry, and quantitative high-content fluorescence imaging. The inflammatory consequences were assessed using quantitative analysis of 13 different chemokines and cytokines in the culture supernatants. Hydrogen peroxide served as the control condition. A modest but significant cytotoxic effect was observed in the metabolic activity and viability after plasma treatment for all three composites. This was only partially treatment time-dependent and the composites alone affected the cells to some extent, as evident by differential secretion profiles of VEGF, for example. Gas plasma composite modification markedly elevated the secretion of IL6, IL8, IL18, and CCL2, with the latter showing the highest correlation with treatment time (Pearson’s r > 0.95). Cell culture media incubated with gas plasma-treated composite chips and added to cells thereafter could not replicate the effects, pointing to the potential that surface modifications elicited the findings. In conclusion, our data suggest that gas plasma treatment modifies composite material surfaces to a certain extent, leading to measurable but overall modest biological effects

Oxidized Proteins Differentially Affect Maturation and Activation of Human Monocyte-Derived Cells

In cancer, antigen-presenting cells (APC), including dendritic cells (DCs), take up and process proteins to mount adaptive antitumor immune responses. This often happens in the context of inflamed cancer, where reactive oxygen species (ROS) are ubiquitous to modify proteins. However, the inflammatory consequences of oxidized protein uptake in DCs are understudied. To this end, we investigated human monocyte-derived cell surface marker expression and cytokine release profiles when exposed to oxidized and native proteins. Seventeen proteins were analyzed, including viral proteins (e.g., CMV and HBV), inflammation-related proteins (e.g., HO1 and HMGB1), matrix proteins (e.g., Vim and Coll), and vastly in the laboratory used proteins (e.g., BSA and Ova). The multifaceted nature of inflammation-associated ROS was mimicked using gas plasma technology, generating reactive species cocktails for protein oxidation. Fourteen oxidized proteins led to elevated surface marker expression levels of CD25, CD40, CD80, CD86, and MHC-II as well as strongly modified release of IL6, IL8, IL10, IL12, IL23, MCP-1, and TNFα compared to their native counterparts. Especially IL8, heme oxygenase 2, and vimentin oxidation gave pronounced effects. Furthermore, protein kinase phospho-array studies in monocyte-derived cells pulsed with native vs. oxidized IL8 and insulin showed enhanced AKT and RSK2 phosphorylation. In summary, our data provide for the first time an overview of the functional consequences of oxidized protein uptake by human monocyte-derived cells and could therefore be a starting point for exploiting such principle in anticancer therapy in the future

Gas plasma–oxidized sodium chloride acts via hydrogen peroxide in a model of peritoneal carcinomatosis

Gas plasma technology generates reactive oxygen and nitrogen species (ROS/RNS), inducing lethal oxidative damage in tumor cells. The transfer of gas plasma–derived ROS/RNS into liquids has been proposed as an innovative anti-cancer strategy targeting peritoneal carcinomatosis (PC). However, the mechanism of action is under debate. To this end, we compared gas plasma–oxidized medical-grade sodium chloride (oxNaCl) with a concentration-matched control (cmc) of NaCl enriched with equivalent concentrations of H2O2 and NO32 in several cell lines and models of PC. Strikingly, oxNaCl and cmc performed equally well in oxidation and cytotoxic activity in tumor cells in two-dimensional cultures, three-dimensional (3D) tumor spheroids, vascularized 3D tumors grown on chicken-embryo chorioallantoic membranes, and a syngeneic PC mouse model in vivo. Given the importance of immunotherapies in oncology today, we focused on immunological consequences of the treatment. Again, to a similar extent, oxNaCl and cmc increased tumor cell immunogenicity and enhanced uptake by and maturation of peripheral blood monocyte–derived dendritic cells together with an inflammatory secretion profile. Furthermore, NanoString gene expression profiling revealed immune system processes and unfolded protein response-related pathways as being linked to the observed anti-tumor effects for both oxNaCl and cmc. In conclusion, gas plasma–generated oxNaCl and cmc showed equal therapeutic efficacy in our PC-related models. In light of the many promising anti-cancer studies of gas plasma–oxidized liquids and the convenient production of corresponding cmcs in large quantities as needed in clinics, our findings may spur research lines based on low-dose oxidants in peritoneal cancer therapy

Argon Plasma Exposure Augments Costimulatory Ligands and Cytokine Release in Human Monocyte-Derived Dendritic Cells

Cold physical plasma is a partially ionized gas expelling many reactive oxygen and nitrogen species (ROS/RNS). Several plasma devices have been licensed for medical use in dermatology, and recent experimental studies suggest their putative role in cancer treatment. In cancer therapies with an immunological dimension, successful antigen presentation and inflammation modulation is a key hallmark to elicit antitumor immunity. Dendritic cells (DCs) are critical for this task. However, the inflammatory consequences of DCs following plasma exposure are unknown. To this end, human monocyte-derived DCs (moDCs) were expanded from isolated human primary monocytes; exposed to plasma; and their metabolic activity, surface marker expression, and cytokine profiles were analyzed. As controls, hydrogen peroxide, hypochlorous acid, and peroxynitrite were used. Among all types of ROS/RNS-mediated treatments, plasma exposure exerted the most notable increase of activation markers at 24 h such as CD25, CD40, and CD83 known to be crucial for T cell costimulation. Moreover, the treatments increased interleukin (IL)-1α, IL-6, and IL-23. Altogether, this study suggests plasma treatment augmenting costimulatory ligand and cytokine expression in human moDCs, which might exert beneficial effects in the tumor microenvironment

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Recent research indicated the potential of cold physical plasma in cancer therapy. The plethora of plasma-derived reactive oxygen and nitrogen species (ROS/RNS) mediate diverse antitumor effects after eliciting oxidative stress in cancer cells. We aimed at exploiting this principle using a newly designed dual-jet neon plasma source (Vjet) to treat colorectal cancer cells. A treatment time-dependent ROS/RNS generation induced oxidation, growth retardation, and cell death within 3D tumor spheroids were found. In TUM-CAM, a semi in vivo model, the Vjet markedly reduced vascularized tumors' growth, but an increase of tumor cell immunogenicity or uptake by dendritic cells was not observed. By comparison, the argon-driven single jet kINPen, known to mediate anticancer effects in vitro, in vivo, and in patients, generated less ROS/RNS and terminal cell death in spheroids. In the TUM-CAM model, however, the kINPen was equivalently effective and induced a stronger expression of immunogenic cancer cell death (ICD) markers, leading to increased phagocytosis of kINPen but not Vjet plasma-treated tumor cells by dendritic cells. Moreover, the Vjet was characterized according to the requirements of the DIN-SPEC 91315. Our results highlight the plasma device-specific action on cancer cells for evaluating optimal discharges for plasma cancer treatment

Energy dependence and fluctuations of anisotropic flow in Pb-Pb collisions at 1asNN = 5.02 and 2.76 TeV

Measurements of anisotropic flow coefficients with two- and multi-particle cu- mulants for inclusive charged particles in Pb\u2013Pb collisions at 1asNN = 5.02 and 2.76 TeV are reported in the pseudorapidity range |\u3b7| < 0.8 and transverse momentum 0.2 < pT < 50 GeV/c. The full data sample collected by the ALICE detector in 2015 (2010), corre- sponding to an integrated luminosity of 12.7 (2.0) \u3bcb 121 in the centrality range 0\u201380%, is analysed. Flow coefficients up to the sixth flow harmonic (v6) are reported and a de- tailed comparison among results at the two energies is carried out. The pT dependence of anisotropic flow coefficients and its evolution with respect to centrality and harmonic number n are investigated. An approximate power-law scaling of the form vn(pT) 3c pn/3 T is observed for all flow harmonics at low pT (0.2 < pT < 3 GeV/c). At the same time, the ratios v /vn/m are observed to be essentially independent of p for most centralities up to nmT about pT = 10 GeV/c. Analysing the differences among higher-order cumulants of elliptic flow (v2), which have different sensitivities to flow fluctuations, a measurement of the stan- dardised skewness of the event-by-event v2 distribution P(v2) is reported and constraints on its higher moments are provided. The Elliptic Power distribution is used to parametrise P(v2), extracting its parameters from fits to cumulants. The measurements are compared to different model predictions in order to discriminate among initial-state models and to constrain the temperature dependence of the shear viscosity to entropy-density ratio

Azimuthally-differential pion femtoscopy relative to the third harmonic event plane in Pb\u2013Pb collisions at 1asNN = 2.76TeV

Azimuthally-differential femtoscopic measurements, being sensitive to spatio-temporal characteristics of the source as well as to the collective velocity fields at freeze out, provide very important information on the nature and dynamics of the system evolution. While the HBT radii oscillations relative to the second harmonic event plane measured recently reflect mostly the spatial geometry of the source, model studies have shown that the HBT radii oscillations relative to the third harmonic event plane are predominantly defined by the velocity fields. In this Letter, we present the first results on azimuthally-differential pion femtoscopy relative to the third harmonic event plane as a function of the pion pair transverse momentum kT for different collision centralities in Pb\u2013Pb collisions at 1asNN = 2.76 TeV. We find that the Rside and Rout radii, which characterize the pion source size in the directions perpendicular and parallel to the pion transverse momentum, oscillate in phase relative to the third harmonic event plane, similar to the results from 3+1D hydrodynamical calculations. The observed radii oscillations unambiguously signal a collective expansion and anisotropy in the velocity fields. A comparison of the measured radii oscillations with the Blast-Wave model calculations indicate that the initial state triangularity is washed- out at freeze out