PENGARUH WORK STRESS TERHADAP KINERJA KARYAWAN DENGAN JOB SATISFACTION SEBAGAI VARIABEL INTERVENING PADA KARYAWAN BAGIAN PRODUKSI PT. GUDANG BARU BERKAH KEPANJEN

This research study to determine employee performance, work stress and job satisfaction and analyze the effect of work stress on employee performance with job satisfaction as an intervening variable. The type of research used in this research is explanatory research with a quantitative approach. The data collection technique in this research uses a questionnaire. This research was conducted at PT. Gudang Baru Berkah Kepanjen. The population in this study were employees of the production department of PT. Gudang Baru Berkah with a total of 120 employees. The sample used in this research was 55 employees using a sampling technique using simple random sampling by taking samples from the population at random without paying attention to strata in the population. The data analysis method used is path analysis with SmartPLS 4.0 software. The results of this research indicate that work stress has a significant effect on employee performance. Work stress has a significant effect on job satisfaction. Job satisfaction does not have a significant effect on employee performance. Work stress does not have a significant effect on employee performance with job satisfaction as an intervening variabl

Uji Kuantitatif flavonoid dan Aktivitas Antioksidan Teh Kombucha Daun Kersen (Muntingia calabura)

Cherry leaf is a plant that contains high antioxidants to help protect the body from cell damage due to free radicals, steeping cherry leaves can be used as a health drink that contains more antioxidants, which is processed into kombucha tea. Kombucha is a fermented beverage made from a solution of tea and sugar by adding kombucha microbial starter and several types of yeast. This research is an experimental study. Determination of total flavonoid content and antioxidant activity test using UV-Vis spectrophotometry method. Flavonoids were determined based on the formation of aluminum chloride reagent complex compound with quercetin standard. Analysis of antioxidant activity test using the DPPH method with quercetin as a comparison. Phytochemical screening results showed that cherry leaf kombucha tea contains flavonoid compounds, tannins, saponins, alkaloids and phenols. The result of testing the total flavonoid content in kombucha tea preparations of cherry leaves was 44,026 mg/g quercetin. The results of the determination of antioxidant activity with the IC50 parameter in cherry leaf kombucha tea were 7.66 ppm with a very strong category.ABSTRAKDaun kersen merupakan tanaman yang mengandung antioksidan tinggi untuk membantu melindungi tubuh dari kerusakan sel akibat radikal bebas. Seduhan daun kersen dapat dimanfaatkan sebagai minuman kesehatan yang mengandung lebih banyak antioksidan yaitu diolah menjadi teh kombucha. Kombucha adalah minuman hasil fermentasi larutan teh dan gula dengan menambahkan starter mikrobia kombucha dan beberapa jenis khamir. Tujuan penelitian ini adalah untuk mengetahui kadar flavonoid total serta aktivitas antioksidan pada teh kombucha daun kersen. Penelitian ini merupakan penelitian eksperimental dengan analisis kadar flavonoid total serta uji aktivitas antioksidan menggunakan metode spektrofotometri UV-Vis. Flavonoid ditetapkan berdasarkan pembentukan senyawa kompleks reagen alumunium klorida dengan standar baku kuersetin. Analisa uji aktivitas antioksidan menggunakan metode DPPH dengan pembanding kuersetin. Hasil skrining fitokimia menunjukkan bahwa teh kombucha daun kersen mengandung senyawa flavonoid, tanin, saponin, alkaloid dan fenol. Hasil pengujian kadar flavonoid total pada sedian teh kombucha daun kersen adalah 44,026 mg/g kuersetin. Hasil penetapan aktivitas antioksidan dengan nilai %inhibisi pada konsentrasi 1 ppm didapatkan 28,28%, 2 ppm didapatkan 30,26%, 3 ppm didapatkan 33,80%, 4 ppm didapatkan 37,19%, 5 ppm didapatkan 41,86% dan nilai IC50 7,66 ppm dengan kategori sangat kuat

Uji Kuantitatif flavonoid dan Aktivitas Antioksidan Teh Kombucha Daun Kersen (Muntingia calabura)

Cherry leaf is a plant that contains high antioxidants to help protect the body from cell damage due to free radicals, steeping cherry leaves can be used as a health drink that contains more antioxidants, which is processed into kombucha tea. Kombucha is a fermented beverage made from a solution of tea and sugar by adding kombucha microbial starter and several types of yeast. This research is an experimental study. Determination of total flavonoid content and antioxidant activity test using UV-Vis spectrophotometry method. Flavonoids were determined based on the formation of aluminum chloride reagent complex compound with quercetin standard. Analysis of antioxidant activity test using the DPPH method with quercetin as a comparison. Phytochemical screening results showed that cherry leaf kombucha tea contains flavonoid compounds, tannins, saponins, alkaloids and phenols. The result of testing the total flavonoid content in kombucha tea preparations of cherry leaves was 44,026 mg/g quercetin. The results of the determination of antioxidant activity with the IC50 parameter in cherry leaf kombucha tea were 7.66 ppm with a very strong category.ABSTRAKDaun kersen merupakan tanaman yang mengandung antioksidan tinggi untuk membantu melindungi tubuh dari kerusakan sel akibat radikal bebas. Seduhan daun kersen dapat dimanfaatkan sebagai minuman kesehatan yang mengandung lebih banyak antioksidan yaitu diolah menjadi teh kombucha. Kombucha adalah minuman hasil fermentasi larutan teh dan gula dengan menambahkan starter mikrobia kombucha dan beberapa jenis khamir. Tujuan penelitian ini adalah untuk mengetahui kadar flavonoid total serta aktivitas antioksidan pada teh kombucha daun kersen. Penelitian ini merupakan penelitian eksperimental dengan analisis kadar flavonoid total serta uji aktivitas antioksidan menggunakan metode spektrofotometri UV-Vis. Flavonoid ditetapkan berdasarkan pembentukan senyawa kompleks reagen alumunium klorida dengan standar baku kuersetin. Analisa uji aktivitas antioksidan menggunakan metode DPPH dengan pembanding kuersetin. Hasil skrining fitokimia menunjukkan bahwa teh kombucha daun kersen mengandung senyawa flavonoid, tanin, saponin, alkaloid dan fenol. Hasil pengujian kadar flavonoid total pada sedian teh kombucha daun kersen adalah 44,026 mg/g kuersetin. Hasil penetapan aktivitas antioksidan dengan nilai %inhibisi pada konsentrasi 1 ppm didapatkan 28,28%, 2 ppm didapatkan 30,26%, 3 ppm didapatkan 33,80%, 4 ppm didapatkan 37,19%, 5 ppm didapatkan 41,86% dan nilai IC50 7,66 ppm dengan kategori sangat kuat

Genetic Characteristics of Actinomycetes Isolates (GST, KP, KP11, Kp16, T24, T37 Code) by RFLP of NRPS Genes

Actinomycetes is the one of antibiotic producing microorganisms. Actinomycetes isolates with GST, KP, KP11, KP16, T24, T37 have been isolated from soil. This study aims to determine the diversity of actinomycetes isolates by RFLP (Restriction Fragment Length Polymorphism) of NRPS (Non Ribosomal Peptide Synthetase) genes from GST, KP, KP11, KP16, T24, and T37 isolates. This study is divided into several steps of the DNA isolation  from isolate GST, KP16, T24, T37, KP, KP11, PCR of the 16S rRNA and NRPS genes and cutting the NRPS genes fragmens with HaeIII enzyme to determine the diversity of isolates. Results of DNA isolate, PCR of 16S rRNA genes and NRPS genes, and RFLP of NRPS genes performed by agarose gel electrophoresis. The diversity of the isolates were analyzed by multivariate analysis UPGMA with MVSP 2.0 applications. The results showed the diversity of 6 actinomycetes isolates are T37 and GST have 64 % similarity, KP11 and KP16 have 96 % similarity, KP has 76 % similarity with KP11 and KP16, while T24 has 60 % similarity with KP and 12 % similarity with T37 and GST. Based on RFLP analysis of the NRPS genes, among of 6 isolates can divided into 4 groups are T37 and GST isolates as group 1, KP11 and KP16 isolates as group 2, KP isolate as group 3, and T24 isolate as group 4. Keyword : Actinomycetes, diversity, 16S rRNA genes, NRPS   genes, RFL

PENGARUH EXPERIENTIAL MARKETING DAN VARIASI PRODUK TERHADAPMINAT BELI ULANG PADA OUTLET STREET BOBA CABANG RAWAMANGUN

The purpose of this study was to determine the effect of experiential marketing and product variations on repurchase interest at the Street Boba outlet of Rawamangun branch. This study used a quantitative approach with data collection techniques in the form of questionnaire distribution. The research sample consisted of 120 respondents who were selected using the nonprobability sampling method. The data collected were analyzed using multiple linear regression analysis with the help of statistical applications IBM SPSS 25. The results showed that experiential marketing and product variations simultaneously have a significant effect on consumer repurchase interest. The value of R square indicates that the experiential marketing and product variation variables have a 72% influence on the consumer repurchase interest variable, with the remaining 28% being the influence of other variables outside this study

Uji Aktivitas Isolat Actinomycetes (Kode Gst, Kp, Kp11, Kp16, T24, Dan T37) Terhadap Staphylococcus Aureus Atcc 25923 Dan Escherichia Coli Atcc 25922

ABSTRAK Prevalensi penyakit infeksi belum menunjukkan penurunan dari tahun ke tahun. Faktor penyebab tingginya kasus infeksi adalah pemakaian antibiotika yang telah resisten. Resistensi mikroba yang meningkat terhadap antibiotik dan munculnya mikroba patogen baru telah mengilhami pencarian antibiotik baru dari mikroba. Salah satu kelompok mikroba yang paling potensial sebagai penghasil senyawa obat yang dicari saat ini adalah Actinomycetes. Actinomycetes dikenal sebagai sumber penting untuk antibiotik dan molekul bioaktif. Penelitian ini bertujuan untuk mengetahui aktivitas isolat Actinomycetes sebagai penghasil antibiotik terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 25922. Hasil isolasi Actinomycetes pada penelitian ini, diberi kode GST, KP, KP11, KP16, T24, dan T37. Pengujian aktivitas antibakteri isolat Actinomycetes dengan metode difusi agar yaitu metode sumuran, dengan kontrol positif menggunakan kloramfenikol 20%. Hasil dari uji aktivitas cairan kultur isolat Actinomycetes yaitu kode KP yang menunjukkan bahwa cairan kultur isolat tersebut mampu menghambat pertumbuhan S. aureus dan E. coli. Zona penghambatan terhadap S. aureus adalah 5 mm, sedangkan pada E. coli memiliki diameter zona hambat sebesar 3,25 mm.Kata Kunci : Actinomycetes, Uji Aktivitas, Staphylococcus aureus, Escherichia coli The prevalence of infectious diseases has not shown a decrease from year to year. The causing factor of infection high cases are antibiotic used that has been resistant. Microbial resistance increased to antibiotics and the emergence of new pathogenic microbes have inspired the research for new antibiotics from microbes. One of the most potential microbial groups as a producer of the drug compounds that are sought at this time is Actinomycetes. Actinomycetes is known as an important source for antibiotics and bioactive molecules. This study aims to determine the activity of Actinomycetes isolates as an antibiotic producer against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The results of the Actinomycetes isolation in this study were given the GST, KP, KP11, KP16, T24, and T37 codes. Antibacterial activity testing of Actinomycetes isolates using diffusion agar method was well method, with positive control using chloramphenicol 20 %. The results of the activity test of the Actinomycetes isolates culture were the KP code which showed that the isolate culture was able to inhibit the growth of S. aureus and E. coli. The inhibitory zone against S.aureus was 5 mm, while at E.coli was 3,25 mm.Keywords : Actinomycetes, Activity Test, Staphylococcus aureus, Escherichia coli

Uji Aktivitas Isolat Actinomycetes (Kode Gst, Kp, Kp11, Kp16, T24, Dan T37) Terhadap Staphylococcus Aureus Atcc 25923 Dan Escherichia Coli Atcc 25922

ABSTRAK Prevalensi penyakit infeksi belum menunjukkan penurunan dari tahun ke tahun. Faktor penyebab tingginya kasus infeksi adalah pemakaian antibiotika yang telah resisten. Resistensi mikroba yang meningkat terhadap antibiotik dan munculnya mikroba patogen baru telah mengilhami pencarian antibiotik baru dari mikroba. Salah satu kelompok mikroba yang paling potensial sebagai penghasil senyawa obat yang dicari saat ini adalah Actinomycetes. Actinomycetes dikenal sebagai sumber penting untuk antibiotik dan molekul bioaktif. Penelitian ini bertujuan untuk mengetahui aktivitas isolat Actinomycetes sebagai penghasil antibiotik terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 25922. Hasil isolasi Actinomycetes pada penelitian ini, diberi kode GST, KP, KP11, KP16, T24, dan T37. Pengujian aktivitas antibakteri isolat Actinomycetes dengan metode difusi agar yaitu metode sumuran, dengan kontrol positif menggunakan kloramfenikol 20%. Hasil dari uji aktivitas cairan kultur isolat Actinomycetes yaitu kode KP yang menunjukkan bahwa cairan kultur isolat tersebut mampu menghambat pertumbuhan S. aureus dan E. coli. Zona penghambatan terhadap S. aureus adalah 5 mm, sedangkan pada E. coli memiliki diameter zona hambat sebesar 3,25 mm.Kata Kunci : Actinomycetes, Uji Aktivitas, Staphylococcus aureus, Escherichia coli The prevalence of infectious diseases has not shown a decrease from year to year. The causing factor of infection high cases are antibiotic used that has been resistant. Microbial resistance increased to antibiotics and the emergence of new pathogenic microbes have inspired the research for new antibiotics from microbes. One of the most potential microbial groups as a producer of the drug compounds that are sought at this time is Actinomycetes. Actinomycetes is known as an important source for antibiotics and bioactive molecules. This study aims to determine the activity of Actinomycetes isolates as an antibiotic producer against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The results of the Actinomycetes isolation in this study were given the GST, KP, KP11, KP16, T24, and T37 codes. Antibacterial activity testing of Actinomycetes isolates using diffusion agar method was well method, with positive control using chloramphenicol 20 %. The results of the activity test of the Actinomycetes isolates culture were the KP code which showed that the isolate culture was able to inhibit the growth of S. aureus and E. coli. The inhibitory zone against S.aureus was 5 mm, while at E.coli was 3,25 mm.Keywords : Actinomycetes, Activity Test, Staphylococcus aureus, Escherichia coliÂ

Peningkatan Pengetahuan dan Keterampilan Ibu dalam Pemantauan Tumbuh Kembang serta Pola Konsumsi Omega 3 pada Anak

Nutrition is one of the determinants of the quality of human resources. Good nutritional conditions can be achieved when the body gets enough nutrients to be consumed to allow physical growth, brain development, and the ability to achieve optimal levels of health. Nutrients that play a vital role in the development process of brain neuron cells for baby intelligence are fatty acids. The purpose of this community service program is to increase the knowledge and skills of the community, especially mothers in monitoring child growth and development, as well as adding an explanation on the importance of providing fatty acid intake, one of which is omega 3, for toddlers and children. The implementation method in this community service program consists of location surveys, coordination meetings of the distribution team per group, socialization of activities, and monitoring and evaluation of the results of the community knowledge program. The result of this community service is that activities run well and smoothly. The material presented received a good response and enthusiasm from the community. Knowledge of the importance of providing omega 3 and skills in monitoring child development can be well received by the community with the results of the participant posttest, 33.33% excellent results, 50% good categories, and 16.67% adequate. The conclusion of this service activity is that the activity has been carried out well, this can be seen from the community enthusiastically following the activity until the end, and community service activities have an impact on the results ranging from very good to sufficient knowledge of skills in monitoring growth and development and the importance of providing omega 3 in children. ABSTRAKGizi merupakan salah satu penentu kualitas sumber daya manusia. Kondisi gizi baik dapat dicapai bila tubuh memperoleh cukup zat gizi yang dikonsumsi sehingga memungkinkan terjadinya pertumbuhan fisik, perkembangan otak, dan kemampuan untuk mencapai tingkat kesehatan optimal. Zat gizi yang berperan vital dalam proses tumbuh kembang sel sel neuron otak untuk kecerdasan bayi adalah asam lemak. Tujuan program pengabdian kepada masyarakat ini adalah untuk meningkatkan pengetahuan dan keterampilan masyarakat, khususnya ibu-ibu dalam pemantauan tumbuh kembang anak, serta ditambah pula adanya pemaparan mengenai pentingnya pemberian asupan asam lemak, salah satunya adalah omega 3, pada balita dan anak. Metode pelaksanaan pada program pengabdian ini terdiri dari survey lokasi, rapat koordinasi tim pembagian per kelompok, sosialisasi kegiatan, serta adanya monitoring dan evaluasi hasil program pengetahuan masyarakat. Hasil dari pengabdian masyarakat ini adalah kegiatan berjalan dengan baik dan lancar. Materi yang disampaikan mendapatkan respon dan antusias yang baik dari masyarakat. Pengetahuan tentang pentingnya pemberian omega 3 serta keterampilan dalam pemantauan tumbuh kembang anak dapat diterima dengan baik oleh masyarakat dengan adanya hasil posttest peserta, hasil sangat baik sebanyak 33,33%, katagori baik sebanyak 50%, dan cukup sebanyak 16,67%. Kesimpulan dari kegiatan pengabdian ini adalah kegiatan telah terlaksana dengan baik, hal ini dapat terlihat dari masyarakat secara antusias mengikuti kegiatan hingga akhir, dan kegiatan pengabdian masyarakat memberikan dampak hasil dengan rentang sangat baik sampai cukup pada pengetahuan keterampilan dalam pemantauan tumbuh kembang dan pentingnya pemberian omega 3 pada anak

Peningkatan Pengetahuan dan Keterampilan Ibu dalam Pemantauan Tumbuh Kembang serta Pola Konsumsi Omega 3 pada Anak

Nutrition is one of the determinants of the quality of human resources. Good nutritional conditions can be achieved when the body gets enough nutrients to be consumed to allow physical growth, brain development, and the ability to achieve optimal levels of health. Nutrients that play a vital role in the development process of brain neuron cells for baby intelligence are fatty acids. The purpose of this community service program is to increase the knowledge and skills of the community, especially mothers in monitoring child growth and development, as well as adding an explanation on the importance of providing fatty acid intake, one of which is omega 3, for toddlers and children. The implementation method in this community service program consists of location surveys, coordination meetings of the distribution team per group, socialization of activities, and monitoring and evaluation of the results of the community knowledge program. The result of this community service is that activities run well and smoothly. The material presented received a good response and enthusiasm from the community. Knowledge of the importance of providing omega 3 and skills in monitoring child development can be well received by the community with the results of the participant posttest, 33.33% excellent results, 50% good categories, and 16.67% adequate. The conclusion of this service activity is that the activity has been carried out well, this can be seen from the community enthusiastically following the activity until the end, and community service activities have an impact on the results ranging from very good to sufficient knowledge of skills in monitoring growth and development and the importance of providing omega 3 in children. ABSTRAKGizi merupakan salah satu penentu kualitas sumber daya manusia. Kondisi gizi baik dapat dicapai bila tubuh memperoleh cukup zat gizi yang dikonsumsi sehingga memungkinkan terjadinya pertumbuhan fisik, perkembangan otak, dan kemampuan untuk mencapai tingkat kesehatan optimal. Zat gizi yang berperan vital dalam proses tumbuh kembang sel sel neuron otak untuk kecerdasan bayi adalah asam lemak. Tujuan program pengabdian kepada masyarakat ini adalah untuk meningkatkan pengetahuan dan keterampilan masyarakat, khususnya ibu-ibu dalam pemantauan tumbuh kembang anak, serta ditambah pula adanya pemaparan mengenai pentingnya pemberian asupan asam lemak, salah satunya adalah omega 3, pada balita dan anak. Metode pelaksanaan pada program pengabdian ini terdiri dari survey lokasi, rapat koordinasi tim pembagian per kelompok, sosialisasi kegiatan, serta adanya monitoring dan evaluasi hasil program pengetahuan masyarakat. Hasil dari pengabdian masyarakat ini adalah kegiatan berjalan dengan baik dan lancar. Materi yang disampaikan mendapatkan respon dan antusias yang baik dari masyarakat. Pengetahuan tentang pentingnya pemberian omega 3 serta keterampilan dalam pemantauan tumbuh kembang anak dapat diterima dengan baik oleh masyarakat dengan adanya hasil posttest peserta, hasil sangat baik sebanyak 33,33%, katagori baik sebanyak 50%, dan cukup sebanyak 16,67%. Kesimpulan dari kegiatan pengabdian ini adalah kegiatan telah terlaksana dengan baik, hal ini dapat terlihat dari masyarakat secara antusias mengikuti kegiatan hingga akhir, dan kegiatan pengabdian masyarakat memberikan dampak hasil dengan rentang sangat baik sampai cukup pada pengetahuan keterampilan dalam pemantauan tumbuh kembang dan pentingnya pemberian omega 3 pada anak

The Study of Organic Fertilizers Application on Two Soybean Varieties in Organic Saturated Soil Culture

Indonesia is facing soybean shortage and the low productivity of soybean production from decreasing acreage. Saturated soil culture (SSC) could be one of the solutions for marginal land with drainage problem. Saturated soil culture is cultivation technology that gives continuous irrigation and maintains water depth constantly and makes soil layer in saturated condition. Farmers can use on-farm inputs that are normally available at the production site. An organic farming system may be able to ensure local and regional food security through continuous production. The study was conducted at Cikarawang Experimental Station of Bogor Agricultural University, Indonesia, from December 2009 to February 2011. The objective of the research was to determine the influence of organic fertilizer application on the productivity of two varieties of soybean, “Anjasmoro” and “Wilis”,    in organic saturated soil culture conducted in two cropping seasons. Prior to the experiment all plots were applied with 2 ton.ha-1 of dolomite, 2 ton.ha-1 of rice hull ash, and 10 ton.ha-1 of chicken manure. The experiment of the first season used split plot design with six replications. The main-plot was organic fertilizer that consisted of chicken manure only (20 ton.ha-1), chicken manure (10 t.ha-1) + Centrosema pubescens (4.2 t.ha-1), chicken manure (10 ton.ha-1) + Tithonia diversifolia (4.2 t.ha-1). The sub-plot was soybean varieties “Anjasmoro” and “Wilis”. The soybean productivity was not affected by the application of organic fertilizers. “Willis” productivity (1.98 t.ha-1) was higher than “Anjasmoro” (1.80 t.ha-1). The experiment of second season used split-split plot design with three replications. The main-plot consisted of 50 and 100% fertilizer rate of the first cropping season; the sub-plot was the same types of organic fertilizer with soybean varieties as sub-sub-plot. Rate of fertilizer, types of organic fertilizer and soybean variety did not affect productivity. Application of 50 and 100% rate of fertilizer in the second season produced 2.41 and 2.55 t.ha-1 of dry seeds, respectively. Soybean plants treated with chicken manure, green manure from C. pubescens and T. diversifolia produced 2.45, 2.50 and 2.49 t.ha-1 of dry seeds, respectively. “Anjasmoro” and “Wilis” produced 2.50 and 2.45 t.ha-1 of dry seeds, respectively. Productivity in the second season was 26.26-36.61% higher than those of the first cropping season