Perancangan Sistem Pemantauan Surat Izin Praktik Tenaga Kesehatan di Dinas Kesehatan Pringsewu Lampung

The design of monitoring system of health workers' practice license in health office in Pringsewu LampungPurposeThe purpose of this study was to design a prototype of a monitoring system of health practice licenses (SIP) of health workers in Pringsewu's local government. MethodsAn action research was conducted with 15 subjects. Data collection was done through in-depth interviews, observations and focus group discussions.ResultsBy order of priority issues, value urgency showed 70%, seriousness 75% and growth 75%, and the ultrasound matrix (urgency, seriousness and growth) showed that it is necessary to design a prototype monitoring SIP (100%), access to online or offline (66.66%), mapping of health workers (60%), and integration and communication with professional organizations and the Ministry of Health of Indonesia (60%). One of the functional testing systems that can be used is the SIP monitoring system. Furthermore, considering the system functional testing system the results showed 58% strongly agreed and 42% agreed with the use of the SIP designed monitoring system. The evaluation of the feasibility of the system shows that the aspect ratio before and after using the prototype was 5 times faster than before using the system. This system provides a positive effect on employee performance and can simplify management of managerial data licensing health professionals. ConclusionThe designed prototype system for monitoring practice licenses has overcome one of the problems in the regulatory process concerning the licensing of health personnel, although it is not developed fully, but the prototype is enough to give change and improved performance at the district health office in Pringsewu

Thermal and photochemical degradation of myoglobin pigments in relation to colour stability of sliced dry-cured Parma ham and sliced dry-cured ham produced with nitrite salt

Lipophilic and hydrophilic extracts of the red pigments from Parma ham and nitrosylated pigment of dry-cured ham produced with nitrite salt were prepared with acetone/water (75/25 v/v %) solution and aqueous phosphate buffer, respectively. The spectral characteristics differed for both the lipophilic and the hydrophilic Parma ham pigment compared with the dry-cured ham produced with nitrite salt. The red lipophilic pigment(s) extractable from Parma ham was(were) found to be very stable towards thermal degradation in acetone/water (75/25 v/v %) solution for temperatures up to 70 °C in contrast to the lipophilic pigment(s) extractable from dry-cured ham produced with nitrite salt, which was(were) found to have an energy of activation of 99 kJ/mol for thermal degradation. In contrast, quantum yields for photodegradation of the lipophilic ham pigments exposed to 366 nm (420 nm) monochromatic light were larger for Parma ham than for nitrite-cured ham [1.6×10–5 (6.9×10–6) versus 1.6×10–6 (2×10–6) mol einstein–1] as determined for acetone/water (75/25 v/v %) solution. In agreement with these findings for the extracted lipophilic pigments, sliced Parma ham showed better colour stability than sliced dry-cured ham produced with nitrite salt, when stored in the dark at low oxygen concentration, in contrast to a faster initial discolouration for Parma ham when exposed to light, as shown for chilled storage for 35 days under retail conditions for the two products each packed at two oxygen levels (0.4 and 21%)

Parameter Genetik Sifat Pertumbuhan Dan Kerapatan Kayu Klon Eucalyptus Pellita F. Muell. Di Dua Tapak Yang Berbeda Di Kalimantan Timur

Industrial forest plantations have an important role in fulfilling the wood demand. Based on global industrial development, the forest plantations industry will increase in the following years. Eucalyptus pellita has become main species in Indonesia forest plantations because it has a short cycle and wood products are suitable to forest industry. The average productivity of E. pellita plantations in Indonesia is still low and high variation. In an effort to increase the productivity, the first step is a better understanding of genetic control on growth and basic density. This study aims to determine the genetic parameters for growth and basic density of E. pellita clones on two different sites. The clonal trials was conducted at two different sites. Both clonal trial are designed using RCBD. The number of clones tested in both trial was 30, 5 blocks and 25 tree/plot. The result of the study showed that the effects of clones vary greatly according the enviromental conditions. The clones- environemntal interaction of growth trait is higher than the basic density. This is in line with genetic parameters of growth trait that are less stable than the basic density. The expected genetic gain of growth trait is higher than the basic density and at the same time there was a weak genetic correlation (there is even a negative) between growth trait and basic density. Therefore carefulness is needed in selecting clones when the two traits are used as selection parameters

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts of Egypt and to determine their antimicrobial resistance. In total, 34-suspected Brucella isolates were recovered from lymph nodes, milk, and fetal abomasal contents of infected cattle, buffaloes, sheep, and goats from nine districts in Egypt. The isolates were identified by microbiological methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Differentiation and genotyping were confirmed using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR. Antimicrobial susceptibility testing against clinically used antimicrobial agents (chloramphenicol, ciprofloxacin, erythromycin, gentamicin, imipenem, rifampicin, streptomycin, and tetracycline) was performed using E-Test. The antimicrobial resistance-associated genes and mutations in Brucella isolates were confirmed using molecular tools. In total, 29 Brucella isolates (eight B. abortus biovar 1 and 21 B. melitensis biovar 3) were identified and typed. The resistance of B. melitensis to ciprofloxacin, erythromycin, imipenem, rifampicin, and streptomycin were 76.2%, 19.0%, 76.2%, 66.7%, and 4.8%, respectively. Whereas, 25.0%, 87.5%, 25.0%, and 37.5% of B. abortus were resistant to ciprofloxacin, erythromycin, imipenem, and rifampicin, respectively. Mutations in the rpoB gene associated with rifampicin resistance were identified in all phenotypically resistant isolates. Mutations in gyrA and gyrB genes associated with ciprofloxacin resistance were identified in four phenotypically resistant isolates of B. melitensis. This is the first study highlighting the antimicrobial resistance in Brucella isolated from different animal species in Egypt. Mutations detected in genes associated with antimicrobial resistance unravel the molecular mechanisms of resistance in Brucella isolates from Egypt. The mutations in the rpoB gene in phenotypically resistant B. abortus isolates in this study were reported for the first time in Egypt

Behaviors of Some Soybean Cultivars (Glycine max L.) Yield to Planting Dates and Different Phosphorus Fertilizer Rates

Soybean production components such as planting date, cultivars and phosphorus fertilizer rates affected soybean yield. Two field experiments conducted in extensive field at El-Gahrbia district, Egypt during 2015 and 2016 seasons. The goal of this research was aimed to investigate the influence of sowing date at 1th May, 15th May and 31th May and three phosphorus fertilization rates, i.e. 0, 37.2 and 74.4 kg P2O5/ha on the performance of three soybean (Glycine max (L.) Merrl) cultivars i.e. Crawford, Giza 22 and Giza 111 on growth, yield and seed quality. The tallest plants, the thick stem, highest branches number/plant, pods number/plant, seed number/pod, weight of 1000 seed and seed yield/ha were recorded from sown early on first May in the both seasons. The tallest plants, the thick stems and highest number of branches/plant were recorded from sown Crawford cultivar. Whereas maximum number of pods/plant and number of seed/pod were found from sown Giza 111 cultivar in the both seasons. Increasing phosphorous fertilizer rates significantly increased all studied cultivars of seed yield and yield components. Tallest plant, the thick stem, the highest branches number/plant, pods number/plant, seed number/pod, weight of1000 seed and seed yield/ha were produced from phosphorous fertilizer at a rate of 74.4 kg P2O5/ha in the first and second seasons, respectively

Synthesis of some new Thienopyrimidine with Benzoxazine, Quinazoline and Azole Moieties

Reaction of thienopyrimidinoyl chloride 3 with  2-aminocyclohexanothiophene-3-carboxamide 4 yielded thienopyrimidine derivative 5 that undergoes cyclodehydration to afford thienopyrimidine 6. The synthesis of benzamidazole 8 and benzoxazole 10 derivatives was achieved by condensation of thienopyrimidinoyl chloride 3 and o-phenylenediamine / o-aminophenol followed by cyclization. Reaction of 4-thio-6-methyl-2-(p-methoxyphenyl)-5-acetylpyrimidine 1 and N(o-carboxyphenyl) chloroacetamide11 yielded pyrimidine derivative 12 that cyclized to benzoxazine 13. Compound 13 was transformed to quinazoline derivatives 14a-c and 16. Reaction of 13 with semicarbazide yielded triazoloquinazoline 18

In-situ Magnesium Diboride Superconducting Thin Films grown by Pulsed Laser Deposition

Superconducting thin films of MgB2 were deposited by Pulsed Laser Deposition on magnesium oxide and sapphire substrates. Samples grown at 450C in an argon buffer pressure of about 10-2 mbar by using a magnesium enriched target resulted to be superconducting with a transition temperature of about 25 K. Film deposited from a MgB2 sintered pellet target in ultra high vacuum conditions showed poor metallic or weak semiconducting behavior and they became superconducting only after an ex-situ annealing in Mg vapor atmosphere. Up to now, no difference in the superconducting properties of the films obtained by these two procedures has been evidenced.Comment: 10 pages, 4 figure

Ascaroside Expression in Caenorhabditis elegans Is Strongly Dependent on Diet and Developmental Stage

Background: The ascarosides form a family of small molecules that have been isolated from cultures of the nematode Caenorhabditis elegans. They are often referred to as “dauer pheromones” because most of them induce formation of long-lived and highly stress resistant dauer larvae. More recent studies have shown that ascarosides serve additional functions as social signals and mating pheromones. Thus, ascarosides have multiple functions. Until now, it has been generally assumed that ascarosides are constitutively expressed during nematode development. Methodology/Principal Findings: Cultures of C. elegans were developmentally synchronized on controlled diets. Ascarosides released into the media, as well as stored internally, were quantified by LC/MS. We found that ascaroside biosynthesis and release were strongly dependent on developmental stage and diet. The male attracting pheromone was verified to be a blend of at least four ascarosides, and peak production of the two most potent mating pheromone components, ascr#3 and asc#8 immediately preceded or coincided with the temporal window for mating. The concentration of ascr#2 increased under starvation conditions and peaked during dauer formation, strongly supporting ascr#2 as the main population density signal (dauer pheromone). After dauer formation, ascaroside production largely ceased and dauer larvae did not release any ascarosides. These findings show that both total ascaroside production and the relative proportions of individual ascarosides strongly correlate with these compounds' stage-specific biological functions. Conclusions/Significance: Ascaroside expression changes with development and environmental conditions. This is consistent with multiple functions of these signaling molecules. Knowledge of such differential regulation will make it possible to associate ascaroside production to gene expression profiles (transcript, protein or enzyme activity) and help to determine genetic pathways that control ascaroside biosynthesis. In conjunction with findings from previous studies, our results show that the pheromone system of C. elegans mimics that of insects in many ways, suggesting that pheromone signaling in C. elegans may exhibit functional homology also at the sensory level. In addition, our results provide a strong foundation for future behavioral modeling studies

Distribution of vacA genotypes in Helicobacter pylori strains isolated from Brazilian adult patients with gastritis, duodenal ulcer or gastric carcinoma

This PCR-based analysis is the first molecular epidemiological study in Brazil testing Helicobacter pylori cagA and vacA distribution in adults with gastric complaints, that includes a large number of carcinoma patients. Multiple-strain infection was identified in 11/13.4% patients. vacA sl-ml and cagA(+) genotypes were the most common in patients with a non-mixed infection. All vacA s1 strains were s1b, so subtyping s1 strains was not useful. vacA s1b-m1 and cagA(+) strains were associated with higher prevalence of peptic ulcer and gastric carcinoma than vacA s2-m2 and cagA(-) ones. in conclusion, cagA and vacA genotyping may have clinical relevance in Brazil. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.Univ Fed Minas Gerais, Mol Biol Lab, Fac Med, BR-30130100 Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Lab Res Bacteriol, Fac Med, BR-30130100 Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Dept Pathol, Fac Med, BR-30130100 Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, BR-04023062 São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, BR-21941590 Rio de Janeiro, BrazilUniv Itauna, Fac Fisioterapia, BR-35680033 Itauna, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol, BR-04023062 São Paulo, BrazilWeb of Scienc