Bioelectricity Buzz

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Requirement of Pax6 for the integration of guidance cues in cell migration

Data accessibility. Cell trajectories data and a summary of directedness and angle values are deposited at Dryad: http://dx.doi.org/10.5061/dryad.53512. Funding MA was funded by an Alban International Research Studentship (code: E07D400602UY).Peer reviewedPublisher PD

Contact-mediated control of radial migration of corneal epithelial cells

We thank Darrin Sheppard and other staff at the University of Aberdeen Medical Research Facility for specialist technical assistance. We thank Patsy D. Goast for overnight microscope monitoring. This work was performed under the Biotechnology and Bioscience Research Council Grant number BB/E015840/1 to JMC.Peer reviewedPublisher PD

Wireless control of nerve growth using bipolar electrodes : a new paradigm in electrostimulation

Acknowledgements The authors want to thank financial contribution from Grants from Fundacion MARATO TV3 2011 (110131), AEI (ref MAT2015-65192-R, RTI2018-097753-B-I00, PID2021-123276OB-I00, CEX2019-000917-S).Peer reviewe

Electric field gradients and bipolar electrochemistry effects on neural growth : A finite element study on immersed electroactive conducting electrode materials

Acknowledgments This work was funded by the European Commission FP6 NEST Program (Contract 028473), RTI2018-097753, MAT2011-24363 and MAT2015-65192-R from the Spanish Science Ministry, La Marató de TV3 Foundation (Identification Number 110131), and Severo Ochoa Programme for Centres of Excellence in R&D (SEV-2015-0496). LI. Abad thanks MINECO for a Ramón y Cajal Contract (RYC-2013-12640). The authors also thank A. Beardo (NanoTransport group from UAB) for useful discussions.Peer reviewedPostprin

Electrical Stimulation Directs Migration, Enhances and Orients Cell Division and Upregulates the Chemokine Receptors CXCR4 and CXCR2 in Endothelial Cells

© 2019 S. Karger AG, Basel.Peer reviewedPostprin

The Direction of Neurite Growth in a Weak DC Electric Field Depends on the Substratum: Contributions of Adhesivity and Net Surface Charge

AbstractWe investigated the influence of the growth surface on the direction ofXenopusspinal neurite growth in the presence of a dc electric field of physiological magnitude. The direction of galvanotropism was determined by the substratum; neurites grew toward the negative electrode (cathode) on untreated Falcon tissue culture plastic or on laminin substrata, which are negatively charged, but neurites growing on polylysine, which is positively charged, turned toward the positive electrode (anode). Growth was oriented randomly on all substrata without an electric field. We tested the hypothesis that the charge of the growth surface was responsible for reversed galvanotropism on polylysine by growing neurons on tissue culture dishes with different net surface charges. Although neurites grew cathodally on both Plastek substrata, the frequency of anodal turning was greater on dishes with a net positive charge (Plastek C) than on those with a net negative charge (Plastek M). The charge of the growth surface therefore influenced the frequency of anodal galvanotropism but a reversal in surface charge was insufficient to reverse galvanotropism completely, possibly because of differences in the relative magnitude of the substratum charge densities. The influence of substratum adhesion on galvanotropism was considered by growing neurites on a range of polylysine concentrations. Growth cone to substratum adhesivity was measured using a blasting assay. Adhesivity and the frequency of anodal turning were graded over the range of polylysine concentrations (0 = 0.1 < 1 < 10 = 100 μg/ml). The direction of neurite growth in an electric field is therefore influenced by both substratum charge and growth cone-to-substratum adhesivity. These data are consistent with the idea that spatial or temporal variation in the expression of adhesion molecules in embryos may interact with naturally occurring electric fields to enhance growth cone pathfinding

The ciliary GTPase Arl13b regulates cell migration and cell cycle progression

Acknowledgments We acknowledge Prof. Tamara Caspary from Emory University for kindly providing the cell lines, Linda Duncan from the University of Aberdeen Ian Fraser Cytometry Center for help with flow cytometry. MP was funded by the Scottish Universities Life Science Alliance (SULSA) and the University of Aberdeen. Funding This work was supported by grants from British Council China (Sino-UK higher Education for PhD studies) to YD and CM, The Carnegie Trust for the Universities of Scotland (70190) and The NHS Grampian Endowment Funds (14/09) to BL, and National Natural Science Foundation of China (31528011) to BL and YD.Peer reviewedPostprin

Ion Channels As Emerging Metabolic Regulators and Therapeutic Targets in Osteoarthritis : Nav1.7 As a Recent Exemplar

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