Data accessibility. Cell trajectories data and a summary of directedness and angle values are deposited at Dryad: http://dx.doi.org/10.5061/dryad.53512. Funding MA was funded by an Alban International Research Studentship (code: E07D400602UY).Peer reviewedPublisher PD

Arocena, Miguel

Collinson, Jon Martin

Rajnicek, Ann M

English

The intricate patterns of cell migration that are found throughout development are generated through a vast array of guidance cues. Responding integratively to distinct, often conflicting, migratory signals is probably crucial for cells to reach their correct destination.Pax6is a master transcription factor with key roles in neural development that include the control of cell migration. In this study, we have investigated the ability of cells derived from cortical neurospheres from wild-type (WT) andPax6−/−mouse embryos to integrate diverging guidance cues. We used two different cues, either separately or in combination: substratum nanogrooves to induce contact guidance, and electric fields (EFs) to induce electrotaxis. In the absence of an EF, both WT andPax6−/−cells aligned and migrated parallel to grooves, and on a flat substrate both showed marked electrotaxis towards the cathode. When an EF was applied in a perpendicular orientation to grooves, WT cells responded significantly to both cues, migrating in highly oblique trajectories in the general direction of the cathode. However,Pax6−/−cells had an impaired response to both cues simultaneously. Our results demonstrate that these neurosphere derived cells have the capacity to integrate diverging guidance cues, which requiresPax6function.</jats:p

Miguel Arocena

Ann M. Rajnicek

Jon Martin Collinson

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Royal Society Open Science

Requirement of<i>Pax6</i>for the integration of guidance cues in cell migration

Data accessibility. Cell trajectories data and a summary of directedness and angle values are deposited at Dryad: http://dx.doi.org/10.5061/dryad.53512. Funding MA was funded by an Alban International Research Studentship (code: E07D400602UY).Peer reviewe

Aberdeen University Research

Requirement of Pax6 for the integration of guidance cues in cell migration

https://aura.abdn.ac.uk/bitstream/2164/9465/1/Requirement_of_Pax6_for.pdf

Requirement of Pax6 for the integration of guidance cues in cell migration

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Aberdeen University Research