Pituitary Adenylate Cyclase Activating Peptide (1-38) and its analog (Acetyl-[Ala15, Ala20] PACAP 38-polyamide) reverse methacholine airway hyperresponsiveness in rats

O objetivo deste estudo foi investigar funcionalmente e estruturalmente efeito broncodilatador do peptídeo ativador da adenilato ciclase pituitária (PACAP1-38) e da acetil-[Ala15, Ala20]PACAP 38-poliamida, potente análogo do PACAP-38, nos ratos desafiados pelo metacolina (MeCh). Ratos Wistar machos foram aleatoriamente divididos em cinco grupos. Grupos 1 e 2, inalando aerossóis de solução salina ou doses crescentes de MeCh (0,5, 1, 2,12, 4,25, 8,5, 17, 34 e 68 mg/L). Os outros grupos recebendo terbutalina (Terb) (250 µg/rato) (10-6M), PACAP-38 (50 µg/rato) (0.1 mM) ou análogo do PACAP-38 (50 µg/rato) associados a MeCh na dose de 4,25 mg/L. A resistência pulmonar total (RL) foi registrada antes e 2 min após a administração de Mech pelo equipamento pneumomultiteste. A administração MeCh induziu aumento significativo e dose dependente (pThe aim of this study was to investigate both functionally and structurally bronchodilator effects of Pituitary adenylate cyclase activating peptide (PACAP38) and acetyl-[Ala15, Ala20] PACAP38-polyamide, a potent PACAP38 analog, in rats challenged by methacholine (MeCh). Male Wistar rats were divided randomly into five groups. Groups 1 and 2 inhaled respectively aerosols of saline or increasing doses of MeCh (0.5, 1, 2.12, 4.25, 8.5, 17, 34 and 68mg/L). The other groups received terbutaline (Terb) (250 µg/rat) (10-6 M), PACAP38 (50 µg/rat) (0.1 mM) or PACAP38 analog (50 µg/rat) associated to MeCh from the dose of 4.25 mg/L. Total lung resistances (RL) were recorded before and 2 min after MeCh administration by pneumomultitest equipment. MeCh administration induced a significant and a dose-dependent increase (

Dieldrin initiates apoptosis in rat thymocytes

1147-1150In vitro <span style="font-size:15.0pt;mso-bidi-font-size: 9.0pt;font-family:" times="" new="" roman","serif";color:black"="">incubation for 6 hr to pesticide dieldrin resulted in a dose-dependent decrease of cell viability comparable to that of dexamethasone. <span style="font-size:14.5pt;mso-bidi-font-size: 8.5pt;font-family:" times="" new="" roman","serif";color:black"="">In vivo experiments also demonstrated that dieldrin administration induced a dose-dependent thymic atrophy which appeared to be mediated by endogenous corticosteroids. Agarose gel electrophorosis analysis, revealed the <span style="font-size: 15.0pt;mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman","serif";="" color:black"="">generation of typical apoptotic oligosomal DNA fragmentation in presence of dieldrin. However, in responsc to high concentrations of pesticide, cells seemed to undergo necrosis pathway. Thus, it may be concluded that dieldrin induced apoptosis in rat thymocytes. </span

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