RAGs and Regulation of Autoantibodies

Autoreactive antibodies are etiologic agents in a number of autoimmune diseases. Like all other antibodies these antibodies are produced in developing B cells by V(D)J recombination in the bone marrow. Three mechanisms regulate autoreactive B cells: deletion, receptor editing, and anergy. Here we review the prevalence of autoantibodies in the initial antibody repertoire, their regulation by receptor editing, and the role of the recombinase proteins (RAG l and RAG2) in this process

Turismo y género: desempeño laboral de la mujer en los organismos turísticos públicos del estado Nueva Esparta

Se determinó el desempeño laboral del género femenino en los organismos públicos relacionados con la actividad turística del estado Nueva Esparta. La metodología es descriptiva y de campo, basada en la revisión bibliográfica, en entrevistas dirigidas a los Gerentes o representantes de estos organismos, en la aplicación de un cuestionario a las mujeres empleadas en ellos. Se revisaron leyes y reglamentos que regulan la participación de la mujer en estos organismos. Se concluye que en relación al perfil de la mujer, estas ocupan cargos relevantes en áreas administrativas y directivas; con experiencia laboral en los organismos relacionados con el turismo, e igualdad de participación con respecto a los hombres. Los representantes de estos organismos consideran positivo el aporte del recurso humano femenino, demostrado en el manejo eficiente de información y habilidades para desempeñar las funciones relacionadas con su cargo.Women´s labor performance was evaluated in public institutions related to tourist activity in Nueva Esparta State. The methodology was descriptive, of field research type, based on the bibliographical review, as well as interviews applied to the managers or representatives of these institutions, and a questionnaire applied to the women who work there. Some laws and regulations that refer to the participation of women in these organisms were reviewed. It is concluded that with respect to the labor profile of women, they hold important positions in administrative and managerial areas; with labor experience in the organisms related to tourist activity, with equal degree of participation as men. The representatives of these organisms consider that the contribution of the female human resource is positive, which they demonstrate through efficient information handling and abilities to perform the functions related to their jobs

HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression

<p>Abstract</p> <p>Background</p> <p>RNA interference is a gene regulatory mechanism that employs small RNA molecules such as microRNA. Previous work has shown that HIV-1 produces TAR viral microRNA. Here we describe the effects of the HIV-1 TAR derived microRNA on cellular gene expression.</p> <p>Results</p> <p>Using a variation of standard techniques we have cloned and sequenced both the 5' and 3' arms of the TAR miRNA. We show that expression of the TAR microRNA protects infected cells from apoptosis and acts by down-regulating cellular genes involved in apoptosis. Specifically, the microRNA down-regulates ERCC1 and IER3, protecting the cell from apoptosis. Comparison to our cloned sequence reveals possible target sites for the TAR miRNA as well.</p> <p>Conclusion</p> <p>The TAR microRNA is expressed in all stages of the viral life cycle, can be detected in latently infected cells, and represents a mechanism wherein the virus extends the life of the infected cell for the purpose of increasing viral replication.</p

Using quality risk management in the plantibody HB-01 manufacturing by transgenic tobacco Plants for vaccine production

The production of biopharmaceuticals by transgenic plants is a promising choice to achieve the multi-kilogram amount of products needed to treat many human diseases. However, this scientific field is still lacking of approved specific guidelines regarding points to consider for manufacturing and application of these products. In such sense, the implementation of new manufacturing processes and quality systems using the quality risks management is recognized as something of prime importance in the current pharmaceutical industry. In this work, we summarize the application of the FMEA method to design the manufacturing process of a plantibody, employed in the hepatitis B vaccine production to ensure the vaccine high quality.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Using quality risk management in the plantibody HB-01 manufacturing by transgenic tobacco Plants for vaccine production

The production of biopharmaceuticals by transgenic plants is a promising choice to achieve the multi-kilogram amount of products needed to treat many human diseases. However, this scientific field is still lacking of approved specific guidelines regarding points to consider for manufacturing and application of these products. In such sense, the implementation of new manufacturing processes and quality systems using the quality risks management is recognized as something of prime importance in the current pharmaceutical industry. In this work, we summarize the application of the FMEA method to design the manufacturing process of a plantibody, employed in the hepatitis B vaccine production to ensure the vaccine high quality.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Plan de negocio para la implementación de una tienda virtual en Lima Metropolitana con productos para la menstruación bajo la modalidad de suscripción

Red Crown es una tienda virtual de productos para la menstruación que funciona bajo la modalidad de suscripción, entregándolos en un empaque apropiado y diferenciado, en la hora, día y lugar programado por la suscriptora. El plan de negocio propone ingresar a la industria de productos de higiene femenina, incorporando elementos novedosos como son un sistema de planes de suscripción y añadiendo valor en la experiencia post-venta, con la finalidad de mejorar la gestión de las mujeres en temas relacionados a la menstruación. En ese sentido, el servicio incluye aspectos diferenciadores como un producto físico que permite ser personalizado según las preferencias y necesidades de las consumidoras, conteniendo una gran variedad de tipos y marcas de toallas higiénicas o tampones; un mix de infusiones variadas; productos gratificantes; consejos y notas informativas relacionadas a la menstruación y un producto adicional relacionado al cuidado personal. Por otro lado, se agrega un servicio digital, que forma parte de la propuesta de post-venta, proponiendo la creación e incorporación de las suscriptoras a una comunidad privada a través de grupos de whatsApp y con acceso periódicos a webinars sobre temas de interés dirigidos por profesionales del rubro

Using quality risk management in the plantibody HB-01 manufacturing by transgenic tobacco Plants for vaccine production

The production of biopharmaceuticals by transgenic plants is a promising choice to achieve the multi-kilogram amount of products needed to treat many human diseases. However, this scientific field is still lacking of approved specific guidelines regarding points to consider for manufacturing and application of these products. In such sense, the implementation of new manufacturing processes and quality systems using the quality risks management is recognized as something of prime importance in the current pharmaceutical industry. In this work, we summarize the application of the FMEA method to design the manufacturing process of a plantibody, employed in the hepatitis B vaccine production to ensure the vaccine high quality.Colegio de Farmacéuticos de la Provincia de Buenos Aire

The MIntAct project—IntAct as a common curation platform for 11 molecular interaction databases

IntAct (freely available at http://www.ebi.ac.uk/intact) is an open-source, open data molecular interaction database populated by data either curated from the literature or from direct data depositions. IntAct has developed a sophisticated web-based curation tool, capable of supporting both IMEx- and MIMIx-level curation. This tool is now utilized by multiple additional curation teams, all of whom annotate data directly into the IntAct database. Members of the IntAct team supply appropriate levels of training, perform quality control on entries and take responsibility for long-term data maintenance. Recently, the MINT and IntAct databases decided to merge their separate efforts to make optimal use of limited developer resources and maximize the curation output. All data manually curated by the MINT curators have been moved into the IntAct database at EMBL-EBI and are merged with the existing IntAct dataset. Both IntAct and MINT are active contributors to the IMEx consortium (http://www.imexconsortium.org

Plasma and CSF biomarkers in a memory clinic: Head-to-head comparison of phosphorylated tau immunoassays

INTRODUCTION: Direct comparisons of the main blood phosphorylated tau immunoassays in memory clinic populations are needed to understand possible differences. METHODS: In the BIODEGMAR study, 197 participants presenting with cognitive complaints were classified into an Alzheimer's disease (AD) or a non-AD cerebrospinal fluid (CSF) profile group, according to their amyloid beta 42/ phosphorylated tau (Aβ42/p-tau) ratio. We performed a head-to-head comparison of nine plasma and nine CSF tau immunoassays and determined their accuracy to discriminate abnormal CSF Aβ42/p-tau ratio. RESULTS: All studied plasma tau biomarkers were significantly higher in the AD CSF profile group compared to the non-AD CSF profile group and significantly discriminated abnormal CSF Aβ42/p-tau ratio. For plasma p-tau biomarkers, the higher discrimination accuracy was shown by Janssen p-tau217 (r = 0.76; area under the curve [AUC] = 0.96), ADx p-tau181 (r = 0.73; AUC = 0.94), and Lilly p-tau217 (r = 0.73; AUC = 0.94). DISCUSSION: Several plasma p-tau biomarkers can be used in a specialized memory clinic as a stand-alone biomarker to detect biologically-defined AD. HIGHLIGHTS: Patients with an Alzheimer's disease cerebrospinal fluid (AD CSF) profile have higher plasma phosphorylated tau (p-tau) levels than the non-AD CSF profile group. All plasma p-tau biomarkers significantly discriminate patients with an AD CSF profile from the non-AD CSF profile group. Janssen p-tau217, ADx p-tau181, and Lilly p-tau217 in plasma show the highest accuracy to detect biologically defined AD. Janssen p-tau217, ADx p-tau181, Lilly p-tau217, Lilly p-tau181, and UGot p-tau231 in plasma show performances that are comparable to their CSF counterparts