Evolución de las lesiones cervicales en mujeres VIH (+). San Pedro Sula, 2010-14.

Estudio descriptivo, retrospectivo. Los principales hallazgos fueron que en las mujeres VIH (+) predomina con mayor la lesión intraepitelial de bajo o de alto grado, con progresión rápida (menor de 12 meses) de lesión inicial. Se concluye que la evolución de la lesión cervical es de progresión rápida en las mujeres VIH (+), por lo que se recomienda control citológico dos veces al año en este grupo de pacientes

Novel SCAMPs Lacking NPF Repeats: Ubiquitous and Synaptic Vesicle-Specific Forms Implicate SCAMPs in Multiple Membrane- Trafficking Functions

In vertebrates, secretory carrier membrane proteins (SCAMPs) 1–3 constitute a family of putative membrane-trafficking proteins composed of cytoplasmic N-terminal sequences with NPF repeats, four central transmembrane regions (TMRs), and a cytoplasmic tail. SCAMPs probably function in endocytosis by recruiting EH-domain proteins to the N-terminal NPF repeats but may have additional functions mediated by their other sequences. We now demonstrate that SCAMPs form a much larger and more heterogeneous protein family than envisioned previously, with an evolutionary conservation extending to invertebrates and plants. Two novel vertebrate SCAMPs (SCAMPs 4 and 5), single SCAMP genes in Caenorhabditis elegans and Drosophila melanogaster, and multiple SCAMPs in Arabidopsis thaliana were identified. Interestingly, the novel SCAMPs 4 and 5 lack the N-terminal NPF repeats that are highly conserved in all other SCAMPs. RNA and Western blotting experiments showed that SCAMPs 1–4 are ubiquitously coexpressed, whereas SCAMP 5 is only detectable in brain where it is expressed late in development coincident with the elaboration of mature synapses. Immunocytochemistry revealed that SCAMP 5 exhibits a synaptic localization, and subcellular fractionations demonstrated that SCAMP 5 is highly enriched in synaptic vesicles. Our studies characterize SCAMPs as a heterogeneous family of putative trafficking proteins composed of three isoforms that are primarily synthesized outside of neurons (SCAMPs 2–4), one isoform that is ubiquitously expressed but highly concentrated on synaptic vesicles (SCAMP 1), and one brain-specific isoform primarily localized to synaptic vesicles (SCAMP 5). The conservation of the TMRs in all SCAMPs with the variable presence of N-terminal NPF repeats suggests that in addition to the role of some SCAMPs in endocytosis mediated by their NPF repeats, all SCAMPs perform a “core” function in membrane traffic mediated by their TMRs

Estudio del nivel de satisfacción de los usuarios con respecto al servicio que se les brinda en los cursos impartidos por el Programa Institucional para la Persona Adulta y Adulta Mayor (PIAM).

Proyecto de graduación (Bachillerato en Administración de Empresa) Instituto Tecnológico de Costa Rica, Escuela de Administración de Empresas, 2014.La presente investigación pretende dar respuesta al nivel de satisfacción de los usuarios con respecto al servicio que se les brinda en los cursos impartidos por el Programa Institucional para la Persona Adulta y Adulta Mayor (PIAM). Es por ello que se busca determinar el nivel de satisfacción en general, analizar la aceptación que ha tenido el programa respecto a la oferta académica, horario, desarrollo de la lección, espacio físico, acceso a instalaciones y general. Así también establecer recomendaciones sobre los cursos, establecer el perfil de usuario, realizar un FODA sobre los cursos e identificar necesidades existentes en los usuarios. Para cumplir con dar respuesta a los objetivos, se realizó una investigación de tipo exploratoria para definir la situación actual del Programa y otra descriptiva, para recolectar la información necesaria, a partir de una encuesta administrada a los usuarios activos en el segundo semestre de 2014. La forma en que se determina la muestra a evaluar se realizó por medio de un muestreo sistemático para determinar los usuarios a los cuales se les aplicó la encuesta. xv Se procedió con el análisis de la información recabada, tabulada y generada en cuadros y gráficos, que reflejaron datos que dan respuesta a los objetivos y se determinó que el nivel de satisfacción de los cursos brindados a los usuarios es excelente, con aspectos que podrían mejorar como la comunicación efectiva de cambios o eventualidades que se puedan suscitar en los cursos, la difusión de sus servicios complementarios a los cursos por efecto del boca a boca entre la comunidad universitaria y en general, la retroalimentación respecto a la demanda de cursos clave que permitan dar satisfacción a los estudiantes, entre otros hallazgos que se obtuvieron en el estudio

Conceptual Photography and Critical Learning of the Visual Arts in the University Context. Educational Experience With Future Teachers

The present research study deepen knowledge on the viewpoints of future primary education teaching professionals regarding issues associated with the educational system and its creative capacity. A study was carried out with 216 students undertaking the Primary Teaching Degree of the University of Granada. Participants were required to develop a conceptual photograph which reflected their thoughts regarding weaknesses of the educational system. The resultant pieces served as a research tool following the performance of formal and content analysis. The future teachers identify weaknesses classified in two broad spheres. The first refers to issues of a political or institutional nature which affect education at a general level. The second refers to teaching methods and education at a more localised or specific level. This is a line of work in art education that serves to stimulate a critical view of the educational system in the training of future teachers

A Poglut1 mutation causes a muscular dystrophy with reduced Notch signaling and satellite cell loss

Skeletal muscle regeneration by muscle satellite cells is a physiological mechanism activated upon muscle damage and regulated by Notch signaling. In a family with autosomal recessive limbgirdle muscular dystrophy, we identified a missense mutation in POGLUT1 (protein O-glucosyltransferase 1), an enzyme involved in Notch posttranslational modification and function. In vitro and in vivo experiments demonstrated that the mutation reduces Oglucosyltransferase activity on Notch and impairs muscle development. Muscles from patients revealed decreased Notch signaling, dramatic reduction in satellite cell pool and a muscle-specific adystroglycan hypoglycosylation not present in patients’ fibroblasts. Primary myoblasts from patients showed slow proliferation, facilitated differentiation, and a decreased pool of quiescent PAX7+ cells. A robust rescue of the myogenesis was demonstrated by increasing Notch signaling. None of these alterations were found in muscles from secondary dystroglycanopathy patients. These data suggest that a key pathomechanism for this novel form of muscular dystrophy is Notch-dependent loss of satellite cells.Junta de Andalucía PI-0017-201

Preservice Primary-School Educators’ Perceptions of the Usefulness of and Problems With Art Education: A Case Study

Art education has traditionally been considered a discipline related to leisure. A case study of primary preservice generalist educators at a Spanish university was conducted to determine whether or not this perspective has evolved. It was of interest to examine whether preservice educators understand the way in which art education develops comprehensive and analytical skills. This study integrated both qualitative and quantitative methodologies to examine future teachers’ perceptions of the usefulness of this subject and the problems it presents. Outcomes indicate that there is still a long way to go to transform preservice primary generalist teachers’ false perceptions related to art teaching