Potency of Papaya Seed Powder (Carica papaya L.) as Chicken Meat and Shrimp Preservative

 Chicken meat and shrimp are perishable food materials. Giving natural preservatives is one way to extend the storage time of food. This study aimed to know the potential of papaya seed powder varieties of ‘Bangkok’ and ‘California’ as preservatives. The sample consisted of two varieties of papaya seed powder with concentrations of 2,4,6 and 8 g. Storage time for chicken meat and shrimp was the third days. Parameters measured were total bacterial colonies, pH va-lues, and organoleptic characteristics (color, aroma and texture) of fresh chicken meat and shrimp. The result showed papaya seed pow-der influences the quality of chicken meat and shrimp. fresh chicken meat and shrimp with papaya seed powder varieties ‘Bangkok’ and ‘California’ had a lower total bacterial colony as much as 9 x 105 until 1x106 compared to control which were 4.2 x 107 - 1.87 x 107. Organoleptic result of chicken meat and shrimp with the treatment of papaya seed powder and stored for three days continue to decrease in term of color, aroma and texture. In chicken meat, the decrease in bacterial growth was still within the limits of microorganism con-tamination quality in animal-based food. However, the same con-centrations of papaya seed powder in the shrimp have not been able to reduce bacterial growth until the limit permitted by SNI.

Karakt£risasi Senyawa Aktif Anti Bakteri Minyak Atsiri Bunga Cengkeh (Syzygium Arimaticum)

Water distillation and purification of drought clove (Syzygium arimaticum) interest yields clove interest essential oils 6,5 % (v/w), rust colored ofchocolate, refractive index (25°C) 1,52874, specific mass (25°C) 1,0636 g/ml and condensation in ethanol 70% (1:2) limpid. Oil of clove haves the character of antibacterial to five test bacteriums ( B. subtilis, B. cereus, S. aureus, P. aeroginosa and E. coli). Result of analysis GCMS shows existence of eugenol compound (72,98%), kariaphilena (10,40%), a-humulena (1,04%) and acetate eugenol ( 15,58%). SpectraphotometerJKTI, VOL. 12, No.1, Juni 2010 analysis UV- Vis existence of absorption at Amaks 207,63 nm (ethanol) and 283,76 nm (eugenol). Analysis FTIR existence of bunch - OH (3543,39 em'), aliphatic C-H (1605,79 - 1766,90 em'), aromatic C=C ( 1433,60 - 1514,74 em'), C-Caril (1433,60-1514,74em-I) and cO (1268,44 em')

Genetic Polymorphism of Calpastatin (CAST) Gene in Pasundan Cattle

The aim of this study was to determine genetic diversity of Calpastatin gene (CAST) in Pasundan cattle. Forty-four fresh blood samples were collected from UPTD BPPT Beef Cattle Ciamis West Java. Genomic DNA samples were extracted using High Salt method. A 523 bp fragment of Calpastatin gene was successfully amplified using PCR method. Genotyping of CAST gene was conducted by PCR-RFLP method using RsaI restriction enzyme (GT^AC). Genotypes and alleles were analyzed using software Cervus 3.0.7. Parameters were observed i.e genotypes and alleles frequencies, heterozygosity observed (Ho) and expected (He), Hardy Weinberg Equilibrium (HWE), and Polymorphic Information Content (PIC). Result showed that three variant genotypes of GG, GC and CC were found and two variant alleles of G and C. Allele G was found higher (0.77) than allele C (0.23). Population of Pasundan cattle was found polymorphism and in the Hardy Weinberg Equilibrium. Polymorphic Information Content (PIC) value showed in a moderate (0.290) condition. Values of Heterozygosity observed and expected were 0.409 and 0.355 respectively. This research concludes that there is polymorphism of CAST gene in Pasundan cattle population and has genetic diversity. This result could be used as early genetic information in exploration of Pasundan cattle

Aplikasi Effective Microorganism 10 (Em10) untuk Pertumbuhan Ikan Lele Sangkuriang (Clarias Gariepinus Var. Sangkuriang) di Kolam Budidaya Lele Jombang, Tangerang

The catfish is a fish that is widely cultivated and consumed in Indonesia. The Catfish growth depand on by the availability of food and water quality. EM4 and EM10 are an example of liquid biofertilizer. The addition of EM4 in the fish pond can help the growth of the fish and maintain of the water quality. While the test of EM10 has not been done on fishery fields. The purpose of this study was to determine the effect of EM10 fertilizers on of sangkuriang catfish (Clarias gariepinus Var. sangkuriang) growth and water quality. This research was conducted in February-March 2014. Research using completely randomized design with 7 treatments and 3 replications. The treatments tested were control (A), EM4 10 ml (B), EM4 20 ml (C), EM4 30 ml (D), EM10 10 ml (E), EM10 20 ml (F), and EM10 30 ml (G ). Analysis of data were using ANOVA followed by Duncan test if there is a real effect (ᾱ = 0.05). EM10 fertilizers at concentration of 20 ml could affect to the specific growth rate on catfish in 7 days maintenance, the concentration of 10 ml at 14 days of villa maintenance and the concentration of 30 ml at 28 days of maintenance. EM10 fertilizers can affect the weight growth of catfish. Fertilizer EM10 can maintain the temperature of the water

Antioxidant Activity From Water Extract Of Kecombrang Flower (Etlingera elatior) Leading To Jelly Candy Formulation

A research on antioxidant activity of kecombrang flower ( Etlingera Elatior) water extract leading tojelly candy formulation was conducted. This experiment aims to elucidate the potential usage ofkecombrang flower as a functional food ingredient. Antioxidant activity of the kecombrang flowerwater extract was determined using Diphenyl Picryl Hidrazyl ( DPPH) method. In order to elucidatethe responsible compound for antioxidant activity, GCMS analysis was undertaken. Water extract ofkecombrang flower have antioxidant activity ( IC50=61,6497 ppm) and based on GCMS analysis itcontained 1-dodekanol ( tR=11,60, area=11,73, similarity at 95 %), 3-metil-1-oxo-2-buten 1-(21,41,51-trihidroxyl phenil) ( tR=13,02, area=3,17, similarity at 57 %) and 1-tetradecene ( tR= 13,26,area=6,03, similarity at 98 %). Formulation of jelly candy using kecombrang flower was designedand based on organoleptic test, C formula ( pink, sample code 763) was the optimum jelly pepermintformula with average score of 3,52

The Potential of Nutmeg’s Microbes (Myristica fragrans Houtt.) as Antagonistic Agents against Rigidoporus microporus

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.      

Identifikasi Jenis Cendawan pada Kelelawar (Ordo Chiroptera) di Kota Tangerang Selatan

Kelelawar merupakan salah satu spesies mamalia yang memiliki habitat cukup berdekatan dengan aktivitas manusia dan mampu terbang dalam jarak jauh. Habitat kelelawar dengan kondisi lembab, suhu rendah dan intensitas cahaya rendah berpotensi sebagai distribusi mikroba dan menyebabkan penyakit yang disebabkan oleh cendawan dan khamir yang ditemukan di kelelawar. Penelitian ini bertujuan untuk mengetahui jenis cendawan pada habitat kelelawar. Metode tangkapan menggunakan kelelawar untuk ditangkap dan dilepaskan dengan mist net dan sampel diambil secarapurposive sampling. Sampel yang dikumpulkan adalah rambut hidung dan air liur di mulut kelelawar. Hasil penelitian menunjukkan bahwa 4 jenis cendawan dan 2 spesies khamir diidentifikasi seperti Aspergillus niger, A. fumigatus, Fusarium sp., Mucor sp., Candida sp. dan Rhodotorula sp. Cendawan dan khamir yang ditemukan di kelelawar belum diketahu efek negatif terhadap kesehatan, namun beberapa genera cendawan memiliki patogen potensial pada organisme hidup lainnya

Uji Aktivitas Antibakteri Ekstrak Air Bunga Kecombrang terhadap Bakteri E. Coli dan S. Aureus sebagai Bahan Pangan Fungsional

The testing has been conducted research on the antibacterial activity of aqueous extracts of flowers kecombrang against E. coli and S. Aureus. This study was aims to provided scientific evidence of excellence kecombrang plants as functional food ingredients. Antibacterial activity assays performed using dispersive method. From the results of antibacterial testing kecombrang flower water extract against E. coli concentration of 20% = 0 mm, 40% = 0 mm, 60% = 4.8 mm, 80% = 5.2 mm, 100% = 7.3 mm and the test bacteria S. aureus concentration of 20% = 8.67 mm, 40% = 9.11 mm, 60% = 12:33 mm, 80% = 12:44 mm, 100% = 13.89 mm

Aplikasi Effective Microorganism 10 (Em10) untuk Pertumbuhan Ikan Lele Sangkuriang (Clarias gariepinus var. Sangkuriang) di Kolam Budidaya Lele Jombang, Tangerang

The catfish is a fish that is widely cultivated and consumed in Indonesia. The Catfish growth depand on by the availability of food and water quality. EM4 and EM10 are an example of liquid biofertilizer. The addition of EM4 in the fish pond can help the growth of the fish and maintain of the water quality. While the test of EM10 has not been done on fishery fields. The purpose of this study was to determine the effect of EM10 fertilizers on of sangkuriang catfish (Clarias gariepinus Var. sangkuriang) growth and water quality. This research was conducted in February-March 2014. Research using completely randomized design with 7 treatments and 3 replications. The treatments tested were control (A), EM4 10 ml (B), EM4 20 ml (C), EM4 30 ml (D), EM10 10 ml (E), EM10 20 ml (F), and EM10 30 ml (G ). Analysis of data were using ANOVA followed by Duncan test if there is a real effect (ᾱ = 0.05). EM10 fertilizers at concentration of 20 ml could affect to the specific growth rate on catfish in 7 days maintenance, the concentration of 10 ml at 14 days of villa maintenance and the concentration of 30 ml at 28 days of maintenance. EM10 fertilizers can affect the weight growth of catfish. Fertilizer EM10 can maintain the temperature of the water

Uji Aktivitas Antibakteri Ekstrak Air Bunga Kecombrang terhadap Bakteri E. coli dan S. aureus sebagai Bahan Pangan Fungsional

The testing has been conducted research on the antibacterial activity of aqueous extracts of flowers kecombrang against E. coli and S. Aureus. This study was aims to provided scientific evidence of excellence kecombrang plants as functional food ingredients. Antibacterial activity assays performed using dispersive method. From the results of antibacterial testing kecombrang flower water extract against E. coli concentration of 20% = 0 mm, 40% = 0 mm, 60% = 4.8 mm, 80% = 5.2 mm, 100% = 7.3 mm and the test bacteria S. aureus concentration of 20% = 8.67 mm, 40% = 9.11 mm, 60% = 12:33 mm, 80% = 12:44 mm, 100% = 13.89 mm