Into the depths of deep eutectic solvents

Ionic liquids (ILs) have been successfully tested in a wide range of applications; however, their high price and complicated synthesis make them infeasible for large scale implementation. A decade ago, a new generation of solvents so called deep eutectic solvents (DESs) was reported for the first time. DESs show similar properties to ILs and they have proven to be an excellent alternative to ILs in many applications where ILs succeeded first. Besides, DESs can be prepared easily and cheaply, with low-cost starting materials, no need of solvents, no atom loss during the formation and no additional purification requirements. The main problem that scientist are facing when they want to use DESs in different applications is the lack of information on the fundamentals of these solvents. Why do they form? How do they form? How do the hydrogen bond donor (HBD) and the hydrogen bond acceptor (HBA) interact? How do DESs interact with other compounds? Can we predict their thermophysical properties? The answer to these questions will provide to researches new insights on the application of these solvents, solving the problem of trial-and-error experimentation. In this work, we present a detailed analysis of molecular interactions and conformational states of two selected DESs: lactic acid – choline chloride (2:1) and glycolic acid – choline chloride (1:1), using density functional theory (DFT) and second-order Møller¿Plesset perturbation theory (MP2) with various basis sets. Theoretical calculations were performed using the GAUSSIAN 03 software package. The obtained results are used to evaluate the nature of interactions between the HBD and HBA and their structural features. Additionally, it will contribute to the better understanding of how DESs are formed

NN Scattering: Chiral Predictions for Asymptotic Observables

We assume that the nuclear potential for distances larger than 2.5 fm is given just by the exchanges of one and two pions and, for the latter, we adopt a model based on chiral symmetry and subthreshold pion-nucleon amplitudes, which contains no free parameters. The predictions produced by this model for nucleon-nucleon observables are calculated and shown to agree well with both experiment and those due to phenomenological potentials.Comment: 16 pages, 12 PS figures included, to appear in Physical Review

Drought impact on forest carbon dynamics and fluxes in Amazonia

In 2005 and 2010 the Amazon basin experienced two strong droughts, driven by shifts in the tropical hydrological regime possibly associated with global climate change, as predicted by some global models. Tree mortality increased after the 2005 drought, and regional atmospheric inversion modelling showed basin-wide decreases in CO2 uptake in 2010 compared with 2011 (ref. 5). But the response of tropical forest carbon cycling to these droughts is not fully understood and there has been no detailed multi-site investigation in situ. Here we use several years of data from a network of thirteen 1-ha forest plots spread throughout South America, where each component of net primary production (NPP), autotrophic respiration and heterotrophic respiration is measured separately, to develop a better mechanistic understanding of the impact of the 2010 drought on the Amazon forest. We find that total NPP remained constant throughout the drought. However, towards the end of the drought, autotrophic respiration, especially in roots and stems, declined significantly compared with measurements in 2009 made in the absence of drought, with extended decreases in autotrophic respiration in the three driest plots. In the year after the drought, total NPP remained constant but the allocation of carbon shifted towards canopy NPP and away from fine-root NPP. Both leaf-level and plot-level measurements indicate that severe drought suppresses photosynthesis. Scaling these measurements to the entire Amazon basin with rainfall data, we estimate that drought suppressed Amazon-wide photosynthesis in 2010 by 0.38 petagrams of carbon (0.23-0.53 petagrams of carbon). Overall, we find that during this drought, instead of reducing total NPP, trees prioritized growth by reducing autotrophic respiration that was unrelated to growth. This suggests that trees decrease investment in tissue maintenance and defence, in line with eco-evolutionary theories that trees are competitively disadvantaged in the absence of growth. We propose that weakened maintenance and defence investment may, in turn, cause the increase in post-drought tree mortality observed at our plots.Gordon and Betty Moore FoundationNatural Environment Research Council (NERC)EU FP7 Amazalert (282664) projectEU FP7GEOCARBON (283080) projectNational Council for Scientific and Technological Development (CNPq, Brazil)ARC - fellowship awardERC - Advanced Investigator AwardRoyal Society - Wolfson Research Merit AwardJackson FoundationJohn Fell Fun

Potential of microbiome-based solutions for agrifood systems

Host-associated microbiomes are central to food production systems and human nutrition and health. Harnessing the microbiome may help increase food and nutrient security, enhance public health, mitigate climate change and reduce land degradation. Although several microbiome solutions are currently under development or commercialized in the agrifood, animal nutrition, biotechnology, diagnostics, pharmaceutical and health sectors , fewer products than expected have been successfully commercialized beyond food processing, and fewer still have achieved wider adoption by farming, animal husbandry and other end-user communities. This creates concerns about the translatability of microbiome research to practical applications. Inconsistent efficiency and reliability of microbiome solutions are major constraints for their commercialization and further development, and demands urgent attention

Growth inhibitory effects of 3′-nitro-3-phenylamino nor-beta-lapachone against HL-60: A redox-dependent mechanism

AbstractIn this study, the cytotoxicity, genotoxicity and early ROS generation of 2,2-dimethyl-(3H)-3-(N-3′-nitrophenylamino)naphtho[1,2-b]furan-4,5-dione (QPhNO2) were investigated and compared with those of its precursor, nor-beta-lapachone (nor-beta), with the main goal of proposing a mechanism of antitumor action. The results were correlated with those obtained from electrochemical experiments held in protic (acetate buffer pH 4.5) and aprotic (DMF/TBABF4) media in the presence and absence of oxygen and with those from dsDNA biosensors and ssDNA in solution, which provided evidence of a positive interaction with DNA in the case of QPhNO2. QPhNO2 caused DNA fragmentation and mitochondrial depolarization and induced apoptosis/necrosis in HL-60 cells. Pre-treatment with N-acetyl-l-cysteine partially abolished the observed effects related to the QPhNO2 treatment, including those involving apoptosis induction, indicating a partially redox-dependent mechanism. These findings point to the potential use of the combination of pharmacology and electrochemistry in medicinal chemistry

Quark Condensate in the Deuteron

We study the changes produced by the deuteron on the QCD quark condensate by means the Feynman-Hellmann theorem and find that the pion mass dependence of the pion-nucleon coupling could play an important role. We also discuss the relation between the many body effect of the condensate and the meson exchange currents, as seen by photons and pions. For pion probes, the many-body term in the physical amplitude differs significantly from that of soft pions, the one linked to the condensate. Thus no information about the many-body term of the condensate can be extracted from the pion-deuteron scattering length. On the other hand, in the Compton amplitude, the relationship with the condensate is a more direct one.Comment: to appear in Physics Review C (19 pages, 3 figures

Produtividade e vigor do maracujazeiro-amarelo plantado em covas e plantio direto sob manejo orgânico.

O objetivo deste trabalho foi avaliar o vigor e a produtividade do maracujazeiro-amarelo plantado em diferentes tamanhos de cova e plantio direto sob manejo orgânico. O experimento foi conduzido de 2005 a 2007, no Setor de Agricultura Ecológica da Universidade Federal do Acre, em delineamento de blocos casualizados, constituídos de cinco tratamentos, quatro repetições e quatro plantas por parcela. Foram avaliados cinco tipos de preparo do solo: T1 cova do tamanho do torrão (0,19 x 0,063m) com adubação em cobertura; T2 cova de 0,30 x 0,30 x 0,30m com adubação de plantio na cova; T3 cova de 0,30 x 0,30 x 0,30m com adubação de plantio em cobertura; T4 cova de 0,50 x 0,50 x 0,50m com adubação de plantio na cova; e T5 cova de 0,50 x 0,50 x 0,50m com adubação de plantio em cobertura. O tamanho da cova e o plantio direto não influenciaram o vigor da planta e a biomassa de raízes. O número de frutos por planta e a produtividade, na segunda e na somatória das duas safras, foram maiores com plantio direto e com covas cúbicas de 0,30m. Após dois anos de cultivo, a densidade do solo foi maior na camada de 0-5cm de profundidade num raio de 20cm da planta para o plantio em covas de 0,50m com adubação na cova e menor para o plantio direto, não havendo diferença entre os demais tratamentos. O plantio direto ou o plantio em covas pequenas com dimensões de 0,30 x 0,30 x 0,30m proporcionou maior produtividade de maracujá que o plantio em covas maiores, mesmo não influenciando o vigor das plantas e a massa seca de raízes

Aspergillus fumigatus MADS-Box Transcription Factor rlmA Is Required for Regulation of the Cell Wall Integrity and Virulence

Microbial Biotechnolog

CHIRAL BACKGROUND FOR THE TWO PION EXCHANGE NUCLEAR POTENTIAL: A PARAMETRIZED VERSION

We argue that the minimal chiral background for the two-pion exchange nucleon-nucleon interaction has nowadays a rather firm conceptual basis, which entitles it to become a standard ingredient of any modern potential. In order to facilitate applications, we present a parametrized version of a configuration space potential derived previously. We then use it to assess the phenomenological contents of some existing NN potentials.Comment: REVTEX style, 16 pages, 5 PostScript figures compressed, tarred and uuencode