Rober J. and Janet R. Pasquesi to Mr. Meredith (1 October 1962)

https://egrove.olemiss.edu/mercorr_pro/1408/thumbnail.jp

Structuring Life After Death: Plant Leachates Promote CO2 Uptake by Regulating Microbial Biofilm Interactions in a Northern Peatland Ecosystem

Shifts in plant functional groups associated with climate change have the potential to influence peatland carbon storage by altering the amount and composition of organic matter available to aquatic microbial biofilms. The goal of this study was to evaluate the potential for plant subsidies to regulate ecosystem carbon flux (CO2) by governing the relative proportion of primary producers (microalgae) and heterotrophic decomposers (heterotrophic bacteria) during aquatic biofilm development in an Alaskan fen. We evaluated biofilm composition and CO2 flux inside mesocosms with and without nutrients (both nitrogen and phosphorus), organic carbon (glucose), and leachates from common peatland plants (moss, sedge, shrub, horsetail). Experimental mesocosms were exposed to either natural sunlight or placed under a dark canopy to evaluate the response of decomposers to nutrients and carbon subsidies with and without algae, respectively. Algae were limited by inorganic nutrients and heterotrophic bacteria were limited by organic carbon. The quality of organic matter varied widely among plants and leachate nutrient content, more so than carbon quality, influenced biofilm composition. By alleviating nutrient limitation of algae, plant leachates shifted the biofilm community toward autotrophy in the light-transparent treatments, resulting in a significant reduction in CO2 emissions compared to the control. Without the counterbalance from algal photosynthesis, a heterotrophic biofilm significantly enhanced CO2 emissions in the presence of plant leachates in the dark. These results show that plants not only promote carbon uptake directly through photosynthesis, but also indirectly through a surrogate, the phototrophic microbes

Nutritional values of wild fruits and consumption by migrant frugivorous birds.

Used 18 fruit species and 11 migrant frugivorous bird species in Illinois. The only seasonal trends in fruit traits were interspecific increases in absolute quantity of K and protein per fruit. Fruit energy content did not differ among species having bicolored vs. monochrome or small vs. large fruit displays. The fruit mass consumed was correlated best with dry pulp mass per fruit, providing significant positive correlations in 6 of 11 frugivorous species. Large fruit size relative to bill size did not appear to affect fruit consumption over the range of fruit sizes and bird species used. Because retained energy was correlated with mass consumed, the fruit pulp mass consumed was in most cases a good index of the energy obtained. Some significant differences occurred in digestive efficiency of a bird species eating different fruit species, and among different bird species eating a single fruit species, but no trends were apparent. Regurgitated seed generally spent less time in a bird than did defecated seeds, facilitating more rapid disposal of seed ballast. Smaller birds defecated only small seeds and regurgitated some small seeds as well as all large ones, whereas larger birds defecated all smaller seeds and many larger ones. Resultant seed shadows thus may depend upon both bird and seed size. -from Author

Skin, hair and beyond: the impact of menopause.

The skin is an endocrine organ and a major target of hormones such as estrogens, androgens and cortisol. Besides vasomotor symptoms (VMS), skin and hair symptoms often receive less attention than other menopausal symptoms despite having a significant negative effect on quality of life. Skin and mucosal menopausal symptoms include dryness and pruritus, thinning and atrophy, wrinkles and sagging, poor wound healing and reduced vascularity, whereas skin premalignant and malignant lesions and skin aging signs are almost exclusively caused by environmental factors, especially solar radiation. Hair menopausal symptoms include reduced hair growth and density on the scalp (diffuse effluvium due to follicular rarefication and/or androgenetic alopecia of female pattern), altered hair quality and structure, and increased unwanted hair growth on facial areas. Hormone replacement therapy (HRT) is not indicated for skin and hair symptoms alone due to the risk–benefit balance, but wider potential benefits of HRT (beyond estrogen’s effect on VMS, bone, breast, heart and blood vessels) to include skin, hair and mucosal benefits should be discussed with women so that they will be able to make the best possible informed decisions on how to prevent or manage their menopausal symptoms.post-print1529 K

Online Data-Driven Safety Certification for Systems Subject to Unknown Disturbances

Deploying autonomous systems in safety critical settings necessitates methods to verify their safety properties. This is challenging because real-world systems may be subject to disturbances that affect their performance, but are unknown a priori. This work develops a safety-verification strategy wherein data is collected online and incorporated into a reachability analysis approach to check in real-time that the system avoids dangerous regions of the state space. Specifically, we employ an optimization-based moving horizon estimator (MHE) to characterize the disturbance affecting the system, which is incorporated into an online reachability calculation. Reachable sets are calculated using a computational graph analysis tool to predict the possible future states of the system and verify that they satisfy safety constraints. We include theoretical arguments proving our approach generates reachable sets that bound the future states of the system, as well as numerical results demonstrating how it can be used for safety verification. Finally, we present results from hardware experiments demonstrating our approach's ability to perform online reachability calculations for an unmanned surface vehicle subject to currents and actuator failures.Comment: 6 pages, 7 figure

Photosynthesis down-regulation precedes carbohydrate accumulation under sink limitation in Citrus

Photosynthesis down-regulation due to an imbalance between sources and sinks in Citrus leaves could be mediated by excessive accumulation of carbohydrates. However, there is limited understanding of the physiological role of soluble and insoluble carbohydrates in photosynthesis regulation and the elements triggering the down-regulation process. In this work, the role of non-structural carbohydrates in the regulation of photosynthesis under a broad spectrum of source-sink relationships has been investigated in the Salustiana sweet orange. Soluble sugar and starch accumulation in leaves, induced by girdling experiments, did not induce down-regulation of the photosynthetic rate in the presence of sinks (fruits). The leaf-to-fruit ratio did not modulate photosynthesis but allocation of photoassimilates to the fruits. The lack of strong sink activity led to a decrease in the photosynthetic rate and starch accumulation in leaves. However, photosynthesis down-regulation due to an excess of total soluble sugars or starch was discarded because photosynthesis and stomatal conductance reduction occurred prior to any significant accumulation of these carbohydrates. Gas exchange and fluorescence parameters suggested biochemical limitations to photosynthesis. In addition, the expression of carbon metabolism-related genes was altered within 24 h when strong sinks were removed. Sucrose synthesis and export genes were inhibited, whereas the expression of ADP-glucose pyrophosphorylase was increased to cope with the excess of assimilates. In conclusion, changes in starch and soluble sugar turnover, but not sugar content per se, could provide the signal for photosynthesis regulation. In these conditions, non-stomatal limitations strongly inhibited the photosynthetic rate prior to any significant increase in carbohydrate levels. © The Author 2011. Published by Oxford University Press. All rights reserved.This work was supported by the Conselleria de Cultura, Educacio i Esport de la Generalitat Valenciana [GV/2007/213 and GV/2009/034].González Nebauer, S.; Renau Morata, B.; Guardiola, JL.; Molina Romero, RV. (2011). Photosynthesis down-regulation precedes carbohydrate accumulation under sink limitation in Citrus. Tree Physiology. 31(2):169-177. doi:10.1093/treephys/tpq103S16917731

Airway Mucin Concentration as a Marker of Chronic Bronchitis

Chronic obstructive pulmonary disease (COPD) is characterized by chronic bronchitic and emphysematous components. In one biophysical model, the concentration of mucin on the airway surfaces is hypothesized to be a key variable that controls mucus transport in healthy persons versus cessation of transport in persons with muco-obstructive lung diseases. Under this model, it is postulated that a high mucin concentration produces the sputum and disease progression that are characteristic of chronic bronchitis

Nuclear envelope protein Lem2 is required for mouse development and regulates MAP and AKT kinases

The nuclear lamina, along with associated nuclear membrane proteins, is a nexus for regulating signaling in the nucleus. Numerous human diseases arise from mutations in lamina proteins, and experimental models for these disorders have revealed aberrant regulation of various signaling pathways. Previously, we reported that the inner nuclear membrane protein Lem2, which is expressed at high levels in muscle, promotes the differentiation of cultured myoblasts by attenuating ERK signaling. Here, we have analyzed mice harboring a disrupted allele for the Lem2 gene (Lemd2). No gross phenotypic defects were seen in heterozygotes, although muscle regeneration induced by cardiotoxin was delayed. By contrast, homozygous Lemd2 knockout mice died by E11.5. Although many normal morphogenetic hallmarks were observed in E10.5 knockout embryos, most tissues were substantially reduced in size. This was accompanied by activation of multiple MAP kinases (ERK1/2, JNK, p38) and AKT. Knockdown of Lem2 expression in C2C12 myoblasts also led to activation of MAP kinases and AKT. These findings indicate that Lemd2 plays an essential role in mouse embryonic development and that it is involved in regulating several signaling pathways. Since increased MAP kinase and AKT/mTORC signaling is found in other animal models for diseases linked to nuclear lamina proteins, LEMD2 should be considered to be another candidate gene for human disease

The Mutant Form of Lamin A that Causes Hutchinson-Gilford Progeria Is a Biomarker of Cellular Aging in Human Skin

Hutchinson-Gilford progeria syndrome (HGPS, OMIM 176670) is a rare disorder characterized by accelerated aging and early death, frequently from stroke or coronary artery disease. 90% of HGPS cases carry the LMNA G608G (GGC>GGT) mutation within exon 11 of LMNA, activating a splice donor site that results in production of a dominant negative form of lamin A protein, denoted progerin. Screening 150 skin biopsies from unaffected individuals (newborn to 97 years) showed that a similar splicing event occurs in vivo at a low level in the skin at all ages. While progerin mRNA remains low, the protein accumulates in the skin with age in a subset of dermal fibroblasts and in a few terminally differentiated keratinocytes. Progerin-positive fibroblasts localize near the basement membrane and in the papillary dermis of young adult skin; however, their numbers increase and their distribution reaches the deep reticular dermis in elderly skin. Our findings demonstrate that progerin expression is a biomarker of normal cellular aging and may potentially be linked to terminal differentiation and senescence in elderly individuals