3,355 research outputs found
OpenSPIM - an open access platform for light sheet microscopy
Light sheet microscopy promises to revolutionize developmental biology by
enabling live in toto imaging of entire embryos with minimal phototoxicity. We
present detailed instructions for building a compact and customizable Selective
Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware
and software platform is shared with the scientific community through a public
website, thereby making light sheet microscopy accessible for widespread use
and optimization to various applications.Comment: 7 pages, 3 figures, 6 supplementary videos, submitted to Nature
Methods, associated public website http://openspim.or
An Electrocorticographic Brain Interface in an Individual with Tetraplegia
Brain-computer interface (BCI) technology aims to help individuals with disability to control assistive devices and reanimate paralyzed limbs. Our study investigated the feasibility of an electrocorticography (ECoG)-based BCI system in an individual with tetraplegia caused by C4 level spinal cord injury. ECoG signals were recorded with a high-density 32-electrode grid over the hand and arm area of the left sensorimotor cortex. The participant was able to voluntarily activate his sensorimotor cortex using attempted movements, with distinct cortical activity patterns for different segments of the upper limb. Using only brain activity, the participant achieved robust control of 3D cursor movement. The ECoG grid was explanted 28 days post-implantation with no adverse effect. This study demonstrates that ECoG signals recorded from the sensorimotor cortex can be used for real-time device control in paralyzed individuals
Essential self-adjointness for combinatorial Schr\"odinger operators II- Metrically non complete graphs
We consider weighted graphs, we equip them with a metric structure given by a
weighted distance, and we discuss essential self-adjointness for weighted graph
Laplacians and Schr\"odinger operators in the metrically non complete case.Comment: Revisited version: Ognjen Milatovic wrote to us that he had
discovered a gap in the proof of theorem 4.2 of our paper. As a consequence
we propose to make an additional assumption (regularity property of the
graph) to this theorem. A new subsection (4.1) is devoted to the study of
this property and some details have been changed in the proof of theorem 4.
Renormalization of minimally doubled fermions
We investigate the renormalization properties of minimally doubled fermions,
at one loop in perturbation theory. Our study is based on the two particular
realizations of Borici-Creutz and Karsten-Wilczek. A common feature of both
formulations is the breaking of hyper-cubic symmetry, which requires that the
lattice actions are supplemented by suitable counterterms. We show that three
counterterms are required in each case and determine their coefficients to one
loop in perturbation theory. For both actions we compute the vacuum
polarization of the gluon. It is shown that no power divergences appear and
that all contributions which arise from the breaking of Lorentz symmetry are
cancelled by the counterterms. We also derive the conserved vector and
axial-vector currents for Karsten-Wilczek fermions. Like in the case of the
previously studied Borici-Creutz action, one obtains simple expressions,
involving only nearest-neighbour sites. We suggest methods how to fix the
coefficients of the counterterms non-perturbatively and discuss the
implications of our findings for practical simulations.Comment: 23 pages, 1 figur
Quantum oscillations from Fermi arcs
When a metal is subjected to strong magnetic field B nearly all measurable
quantities exhibit oscillations periodic in 1/B. Such quantum oscillations
represent a canonical probe of the defining aspect of a metal, its Fermi
surface (FS). In this study we establish a new mechanism for quantum
oscillations which requires only finite segments of a FS to exist. Oscillations
periodic in 1/B occur if the FS segments are terminated by a pairing gap. Our
results reconcile the recent breakthrough experiments showing quantum
oscillations in a cuprate superconductor YBCO, with a well-established result
of many angle resolved photoemission (ARPES) studies which consistently
indicate "Fermi arcs" -- truncated segments of a Fermi surface -- in the normal
state of the cuprates.Comment: 8 pages, 5 figure
Analysis of motoneuron responses to composite synaptic volleys (computer simulation study)
This paper deals with the analysis of changes in motoneuron (MN) firing evoked by repetitively applied stimuli aimed toward extracting information about the underlying synaptic volleys. Spike trains were obtained from computer simulations based on a threshold-crossing model of tonically firing MN, subjected to stimulation producing postsynaptic potentials (PSPs) of various parameters. These trains were analyzed as experimental results, using the output measures that were previously shown to be most effective for this purpose: peristimulus time histogram, raster plot and peristimulus time intervalgram. The analysis started from the effects of single excitatory and inhibitory PSPs (EPSPs and IPSPs). The conclusions drawn from this analysis allowed the explanation of the results of more complex synaptic volleys, i.e., combinations of EPSPs and IPSPs, and the formulation of directions for decoding the results of human neurophysiological experiments in which the responses of tonically firing MNs to nerve stimulation are analyzed
Early Detection of Erlotinib Treatment Response in NSCLC by 3′-Deoxy-3′-[18F]-Fluoro-L-Thymidine ([18F]FLT) Positron Emission Tomography (PET)
Background: Inhibition of the epidermal growth factor receptor (EGFR) has shown clinical success in patients with advanced non-small cell lung cancer (NSCLC). Somatic mutations of EGFR were found in lung adenocarcinoma that lead to exquisite dependency on EGFR signaling; thus patients with EGFR-mutant tumors are at high chance of response to EGFR inhibitors. However, imaging approaches affording early identification of tumor response in EGFR-dependent carcinomas have so far been lacking. Methodology/Principal Findings: We performed a systematic comparison of 3′-Deoxy-3′-[]-fluoro-L-thymidine ([]FLT) and 2-[]-fluoro-2-deoxy-D-glucose ([]FDG) positron emission tomography (PET) for their potential to identify response to EGFR inhibitors in a model of EGFR-dependent lung cancer early after treatment initiation. While erlotinib-sensitive tumors exhibited a striking and reproducible decrease in []FLT uptake after only two days of treatment, []FDG PET based imaging revealed no consistent reduction in tumor glucose uptake. In sensitive tumors, a decrease in []FLT PET but not []FDG PET uptake correlated with cell cycle arrest and induction of apoptosis. The reduction in []FLT PET signal at day 2 translated into dramatic tumor shrinkage four days later. Furthermore, the specificity of our results is confirmed by the complete lack of []FLT PET response of tumors expressing the T790M erlotinib resistance mutation of EGFR. Conclusions: []FLT PET enables robust identification of erlotinib response in EGFR-dependent tumors at a very early stage. []FLT PET imaging may represent an appropriate method for early prediction of response to EGFR TKI treatment in patients with NSCLC
Obesity and immune function relationships.
The immunological processes involved in the collaborative defence of organisms are affected by nutritional status. Thus, a positive chronic imbalance between energy intake and expenditure leads to situations of obesity, which may influence unspecific and specific immune responses mediated by humoral and cell mediated mechanisms. Furthermore, several lines of evidence have supported a link between adipose tissue and immunocompetent cells. This interaction is illustrated in obesity, where excess adiposity and impaired immune function have been described in both humans and genetically obese rodents. However, limited and often controversial information exist comparing immunity in obese and non-obese subjects as well as about the cellular and molecular mechanisms implicated. In general terms, clinical and epidemiological data support the evidence that the incidence and severity of specific types of infectious illnesses are higher in obese persons as compared to lean individuals together with the occurrence of poor antibody responses to antigens in overweight subjects. Leptin might play a key role in linking nutritional status with T-cell function. The complexities and heterogeneity of the host defences concerning the immune response in different nutritional circumstances affecting the energy balance require an integral study of the immunocompetent cells, their subsets and products as well as specific and unspecific inducer/regulator systems. In this context, more research is needed to clarify the clinical implications of the alterations induced by obesity on the immune function
Dietary supplement increases plasma norepinephrine, lipolysis, and metabolic rate in resistance trained men
Correction to Richard J Bloomer, Kelsey H Fisher-Wellman, Kelley G Hammond, Brian K Schilling, Adrianna A Weber and Bradford J Cole: Dietary supplement increases plasma norepinephrine, lipolysis, and metabolic rate in resistance trained men. Journal of the International Society of Sports Nutrition 2009, 6:
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