Reconstructing galaxy fundamental distributions and scaling relations from photometric redshift surveys. Applications to the SDSS early-type sample

Noisy distance estimates associated with photometric rather than spectroscopic redshifts lead to a mis-estimate of the luminosities, and produce a correlated mis-estimate of the sizes. We consider a sample of early-type galaxies from the SDSS DR6 for which both spectroscopic and photometric information is available, and apply the generalization of the V_max method to correct for these biases. We show that our technique recovers the true redshift, magnitude and size distributions, as well as the true size-luminosity relation. We find that using only 10% of the spectroscopic information randomly spaced in our catalog is sufficient for the reconstructions to be accurate within about 3%, when the photometric redshift error is dz = 0.038. We then address the problem of extending our method to deep redshift catalogs, where only photometric information is available. In addition to the specific applications outlined here, our technique impacts a broader range of studies, when at least one distance-dependent quantity is involved. It is particularly relevant for the next generation of surveys, some of which will only have photometric information.Comment: 14 pages, 12 figures, 1 table, new section 3.1 and appendix added, MNRAS in pres

Regulation of Cyclooxygenase-2 Expression by Heat: A Novel Aspect of Heat Shock Factor 1 Function in Human Cells

The heat-shock response, a fundamental defense mechanism against proteotoxic stress, is regulated by a family of heat-shock transcription factors (HSF). In humans HSF1 is considered the central regulator of heat-induced transcriptional responses. The main targets for HSF1 are specific promoter elements (HSE) located upstream of heat-shock genes encoding cytoprotective heat-shock proteins (HSP) with chaperone function. In addition to its cytoprotective function, HSF1 was recently hypothesized to play a more complex role, regulating the expression of non-HSP genes; however, the non-canonical role of HSF1 is still poorly understood. Herein we report that heat-stress promotes the expression of cyclooxygenase-2 (COX-2), a key regulator of inflammation controlling prostanoid and thromboxane synthesis, resulting in the production of high levels of prostaglandin-E2 in human cells. We show that heat-induced COX-2 expression is regulated at the transcriptional level via HSF1-mediated signaling and identify, by in-vitro reporter gene activity assay and deletion-mutant constructs analysis, the COX-2 heat-responsive promoter region and a new distal cis-acting HSE located at position −2495 from the transcription start site. As shown by ChIP analysis, HSF1 is recruited to the COX-2 promoter rapidly after heat treatment; by using shRNA-mediated HSF1 suppression and HSE-deletion from the COX-2 promoter, we demonstrate that HSF1 plays a central role in the transcriptional control of COX-2 by heat. Finally, COX-2 transcription is also induced at febrile temperatures in endothelial cells, suggesting that HSF1-dependent COX-2 expression could contribute to increasing blood prostaglandin levels during fever. The results identify COX-2 as a human non-classical heat-responsive gene, unveiling a new aspect of HSF1 function

CARACTERÍSTICAS SOCIODEMOGRÁFICAS Y MORTALIDAD MATERNA EN UN HOSPITAL DE REFERENCIA EN LA CIUDAD DE CURITIBA – PARANÁ

A mortalidade materna atrai inúmeras discussões e preocupações no Brasil visto que reflete a qualidade da assistência prestada nos serviços de saúde, bem como a operacionalização das políticas públicas voltadas à saúde da mulher. Esta pesquisa teve como objetivo identificar o perfil sociodemográfico e causal da mortalidade materna no período de 2004 a 2007, em um hospital de ensino da cidade de Curitiba, Estado do Paraná. Após análise das fichas hospitalares de investigação confidencial de óbitos e dos pareceres técnicos do Comitê Estadual de Prevenção de Mortalidade Materna encontrou-se um percentual de 80,6% de óbitos evitáveis, sendo que 45,16% destes ocorreram devido às causas diretas de óbito materno. Dentre essas causas, as que mais provocaram óbitos foram aquelas relacionadas a doenças do aparelho circulatório, embolias obstétricas e doenças renais. O estudo possibilitou concluir que há deficiência nos registros de informações nos prontuários quanto aos dados sociodemográficos, bem como a necessidade de discutir a melhoria de acesso aos serviços de saúde com vistas a reduzir as taxas de mortalidade materna.Maternal mortality is in the center of many discussions and is of concern in Brazil since it reflects the quality of care in health services, as well as the operationalization of public policies related to women’s health. This research aimed to identify the sociodemographic and causal profile of maternal mortality in the period 2004-2007 in a teaching hospital in Curitiba, Parana State. After analysis of the hospital records during confidential investigation of deaths and technical statements by experts of the State Committee for Prevention of Maternal Mortality, it was found a percentage of 80.6% of avoidable deaths, of which 45.16% of these were due to direct causes of mother deaths. Among these causes, the ones which caused more deaths were those related to circulatory diseases, obstetric embolism and kidney disease. We concluded that there’s some deficiency in the information registries in the medical record regarding socio demographic data, and there’s a need to discuss the health care acess improvement in order to decrease the maternal mortality rates.La mortalidad materna atrae innúmeras discusiones y preocupaciones en Brasil ya que refleja la cualidad de la asistencia prestada en los servicios de salud, así como operacionaliza las políticas públicas para la salud de la mujer. Esta investigación tuvo como objetivo identificar el perfil sociodemográfico y causal de la mortalidad materna en periodo de 2004 a 2007, en un hospital de enseñanza de la ciudad de Curitiba, estado de Paraná. Después del análisis de las fichas hospitalares de investigación confidencial de óbitos y de los pareceres técnicos del Comité Estadual de Prevención de Mortalidad Materna, fue encuentrado un percentual de 80,6% de óbitos evitables, siendo 45,16% de estes ocurridos por causas directas de óbito materno. Entre esas causas, las que más provocaron óbitos fueron aquellas referentes a enfermedades del aparato circulatorio, embolias obstétricas y enfermedades renales. Nosotros concluimos que existe deficiencia en los registros de informaciones en los prontuarios cuanto a los datos sociodemográficos, así como a la necesidad de discutir la mejoría de acceso a los servicios de salud para disminuir los índices de mortalidad matern

Ischemic Stroke Caused by Paradoxical Embolism After an Unsuccessful Transcatheter Atrial Septal Defect Closure Procedure: A Word of Caution

Transcatheter device closure of atrial septal defect (ASD) has become a well-accepted alternative to surgical repair. Serious complications of transcatheter ASD closure are rare, but when they occur, devastating consequences may result. Herein, we present the case of a 4-year-old girl who had an ischemic stroke caused by a presumptive paradoxical embolism after an unsuccessful transcatheter ASD procedure and in whom subsequent venous color Doppler showed deep venous thrombosis (DVT) of the right lower extremity. The risk factors that predisposed to paradoxical cerebral embolism and DVT in this patient are discussed, and the literature is reviewed

Impairment of Auditory-Motor Timing and Compensatory Reorganization after Ventral Premotor Cortex Stimulation

Integrating auditory and motor information often requires precise timing as in speech and music. In humans, the position of the ventral premotor cortex (PMv) in the dorsal auditory stream renders this area a node for auditory-motor integration. Yet, it remains unknown whether the PMv is critical for auditory-motor timing and which activity increases help to preserve task performance following its disruption. 16 healthy volunteers participated in two sessions with fMRI measured at baseline and following rTMS (rTMS) of either the left PMv or a control region. Subjects synchronized left or right finger tapping to sub-second beat rates of auditory rhythms in the experimental task, and produced self-paced tapping during spectrally matched auditory stimuli in the control task. Left PMv rTMS impaired auditory-motor synchronization accuracy in the first sub-block following stimulation (p<0.01, Bonferroni corrected), but spared motor timing and attention to task. Task-related activity increased in the homologue right PMv, but did not predict the behavioral effect of rTMS. In contrast, anterior midline cerebellum revealed most pronounced activity increase in less impaired subjects. The present findings suggest a critical role of the left PMv in feed-forward computations enabling accurate auditory-motor timing, which can be compensated by activity modulations in the cerebellum, but not in the homologue region contralateral to stimulation

Dental management considerations for the patient with an acquired coagulopathy. Part 1: Coagulopathies from systemic disease

Current teaching suggests that many patients are at risk for prolonged bleeding during and following invasive dental procedures, due to an acquired coagulopathy from systemic disease and/or from medications. However, treatment standards for these patients often are the result of long-standing dogma with little or no scientific basis. The medical history is critical for the identification of patients potentially at risk for prolonged bleeding from dental treatment. Some time-honoured laboratory tests have little or no use in community dental practice. Loss of functioning hepatic, renal, or bone marrow tissue predisposes to acquired coagulopathies through different mechanisms, but the relationship to oral haemostasis is poorly understood. Given the lack of established, science-based standards, proper dental management requires an understanding of certain principles of pathophysiology for these medical conditions and a few standard laboratory tests. Making changes in anticoagulant drug regimens are often unwarranted and/or expensive, and can put patients at far greater risk for morbidity and mortality than the unlikely outcome of postoperative bleeding. It should be recognised that prolonged bleeding is a rare event following invasive dental procedures, and therefore the vast majority of patients with suspected acquired coagulopathies are best managed in the community practice setting

Rituximab in B-Cell Hematologic Malignancies: A Review of 20 Years of Clinical Experience

Rituximab is a human/murine, chimeric anti-CD20 monoclonal antibody with established efficacy, and a favorable and well-defined safety profile in patients with various CD20-expressing lymphoid malignancies, including indolent and aggressive forms of B-cell non-Hodgkin lymphoma. Since its first approval 20 years ago, intravenously administered rituximab has revolutionized the treatment of B-cell malignancies and has become a standard component of care for follicular lymphoma, diffuse large B-cell lymphoma, chronic lymphocytic leukemia, and mantle cell lymphoma. For all of these diseases, clinical trials have demonstrated that rituximab not only prolongs the time to disease progression but also extends overall survival. Efficacy benefits have also been shown in patients with marginal zone lymphoma and in more aggressive diseases such as Burkitt lymphoma. Although the proven clinical efficacy and success of rituximab has led to the development of other anti-CD20 monoclonal antibodies in recent years (e.g., obinutuzumab, ofatumumab, veltuzumab, and ocrelizumab), rituximab is likely to maintain a position within the therapeutic armamentarium because it is well established with a long history of successful clinical use. Furthermore, a subcutaneous formulation of the drug has been approved both in the EU and in the USA for the treatment of B-cell malignancies. Using the wealth of data published on rituximab during the last two decades, we review the preclinical development of rituximab and the clinical experience gained in the treatment of hematologic B-cell malignancies, with a focus on the well-established intravenous route of administration. This article is a companion paper to A. Davies, et al., which is also published in this issue

Toxicity in mice expressing short hairpin RNAs gives new insight into RNAi

Short hairpin RNAs can provide stable gene silencing via RNA interference. Recent studies have shown toxicity in vivo that appears to be related to saturation of the endogenous microRNA pathway. Will these findings limit the therapeutic use of such hairpins

The Role of Dicer Protein Partners in the Processing of MicroRNA Precursors

One of the cellular functions of the ribonuclease Dicer is to process microRNA precursors (pre-miRNAs) into mature microRNAs (miRNAs). Human Dicer performs this function in cooperation with its protein partners, AGO2, PACT and TRBP. The exact role of these accessory proteins in Dicer activity is still poorly understood. In this study, we used the northern blotting technique to investigate pre-miRNA cleavage efficiency and specificity after depletion of AGO2, PACT and TRBP by RNAi. The results showed that the inhibition of either Dicer protein partner substantially affected not only miRNA levels but also pre-miRNA levels, and it had a rather minor effect on the specificity of Dicer cleavage. The analysis of the Dicer cleavage products generated in vitro revealed the presence of a cleavage intermediate when pre-miRNA was processed by recombinant Dicer alone. This intermediate was not observed during pre-miRNA cleavage by endogenous Dicer. We demonstrate that AGO2, PACT and TRBP were required for the efficient functioning of Dicer in cells, and we suggest that one of the roles of these proteins is to assure better synchronization of cleavages triggered by two RNase III domains of Dicer

Cervical cancer screening in women vaccinated against human papillomavirus infection: Recommendations from a consensus conference

In Italy, the cohorts of women who were offered Human papillomavirus (HPV) vaccination in 2007/08 will reach the age (25&nbsp;years) for cervical cancer (CC) screening from 2017. The simultaneous shift from cytology-based screening to HPV test-based screening gives the opportunity for unprecedented reorganisation of CC prevention. The ONS (National Screening Monitoring Centre) Directive and the GISCi (Italian Group for Cervical Screening) identified the consensus conference as the most suitable method for addressing this topic. A summary of consensus recommendations is reported here. The main objective was to define the best screening methods in girls vaccinated against HPV and the knowledge required for defining evidence-based screening strategies. A Jury made recommendations about questions and proposals formulated by a panel of experts representative of Italian scientific societies involved in CC prevention and based on systematic reviews of literature and evidence. The Jury considered changing the screening protocols for girls vaccinated in their twelfth year as appropriate. Tailored screening protocols based on vaccination status could be replaced by \u201cone size fits all\u201d protocols only when a herd immunity effect has been reached. Vaccinated women should start screening at age 30, instead of 25, with HPV test. Furthermore, there is a strong rationale for applying longer intervals for re-screening HPV negative women than the currently recommended 5&nbsp;years, but research is needed to determine the optimal screening time points. For non-vaccinated women and for women vaccinated in their fifteenth year or later, the current protocol should be kept