An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation

Human rhinoviruses (HRV) cause the majority of common colds and acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD). Effective therapies are urgently needed, but no licensed treatments or vaccines currently exist. Of the 100 identified serotypes, ∼90% bind domain 1 of human intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, making this an attractive target for development of therapies; however, ICAM-1 domain 1 is also required for host defence and regulation of cell trafficking, principally via its major ligand LFA-1. Using a mouse anti-human ICAM-1 antibody (14C11) that specifically binds domain 1 of human ICAM-1, we show that 14C11 administered topically or systemically prevented entry of two major groups of rhinoviruses, HRV16 and HRV14, and reduced cellular inflammation, pro-inflammatory cytokine induction and virus load in vivo. 14C11 also reduced cellular inflammation and Th2 cytokine/chemokine production in a model of major group HRV-induced asthma exacerbation. Interestingly, 14C11 did not prevent cell adhesion via human ICAM-1/LFA-1 interactions in vitro, suggesting the epitope targeted by 14C11 was specific for viral entry. Thus a human ICAM-1 domain-1-specific antibody can prevent major group HRV entry and induction of airway inflammation in vivo

Sympathy and Tenderness as Components of Dispositional Empathic Concern: Predicting Helping and Caring Behaviors

Recently, empathic concern was separated into the components of sympathy and tenderness (Lishner, Batson, & Huss, 2011). So far, these two emotional experiences have been assessed as episodic emotional responses, as the existent dispositional measures remain blind to such distinction. The aim of the present research is to develop and validate a dispositional measure that captures the personal disposition to feel sympathy, tenderness, and personal distress. This new scale is called Sympathy, Tenderness and Distress Dispositional Scale (SyTeD). In Study 1, we developed and tested the internal consistency and factor structure of the English version of the scale in the United States. In Study 2, we translated the scale into Spanish and tested its content and criterion validity in Spain. In Study 3, we tested the predictive validity of the sympathy-tenderness distinction within a helping vs. a care-based scenario in the United Kingdom (SyTeD-English version). In Study 4, we tested the predictive validity of the sympathy-tenderness distinction in a real helping situation in Spain (SyTeD-Spanish version). The results across these four studies suggest that the SyTeD is a useful measure of dispositional sympathy and tenderness that allows studying further different types of prosocial behavior (i.e., help vs. care)

Bioactive steroids as contaminants of the common carbon source galactose

Most inducible expression vectors for the budding yeast Saccharomyces cerevisiae are based on galactose-inducible promoters. Yeast has been increasingly used to study vertebrate steroid receptors because of its powerful genetics. In principle, both regulatory systems are compatible and can be combined in the same strain. However, we found that commercial galactose can be contaminated by bioactive estrogen and progesterone at concentrations that are sufficient to fully activate their cognate receptors. Since steroids can elicit biological responses in pathogenic fungi and possibly other microorganisms, such contaminants in a commonly used fermentable carbon source may need to be screened fo