Differentation of species within Section Juniperus of Genus Juniperus L. (Cupressaceae) from the Balkan peninsula using phytochemical & molecular characters

U ovom radu analizirana je fitohemijska i molekularna varijabilnost i diferencijacija prirodnih populacija taksona sekcije Juniperus roda Juniperus (Cupressaceae) sa Balkanskog poluostrva...This thesis deals with phytochemical & molecular variability variability & differentation of natural population of taxa belonging to section Juniperus of the genus Juniperus (Cupressaceae) from the Balkan peninsula..

Supplementary data for article: Dodoš, T.; Rajčević, N.; Tešević, V.; Marin, P. D. Chemodiversity of Epicuticular N-Alkanes and Morphological Traits of Natural Populations of Satureja Subspicata Bartl. Ex Vis. along Dinaric Alps – Ecological and Taxonomic Aspects. Chemistry and Biodiversity 2017, 14 (2). https://doi.org/10.1002/cbdv.201600201

Supplementary material for: [https://doi.org/10.1002/cbdv.201600201]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2416

Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data

Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.Ministry of Education, Science, and Technology of the Republic of Serbia {[}173029, 173030

Supplementary data for article: Dodoš, T.; Rajčević, N.; Tešević, V.; Marin, P. D. Chemodiversity of Epicuticular N-Alkanes and Morphological Traits of Natural Populations of Satureja Subspicata Bartl. Ex Vis. along Dinaric Alps – Ecological and Taxonomic Aspects. Chemistry and Biodiversity 2017, 14 (2). https://doi.org/10.1002/cbdv.201600201

Supplementary material for: [https://doi.org/10.1002/cbdv.201600201]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2416

Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts

We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further

Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities

Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry

Supplementary material for the article: Dodoš, T.; Rajčević, N.; Janaćković, P.; Vujisić, L.; Marin, P. D. Essential Oil Profile in Relation to Geographic Origin and Plant Organ of Satureja Kitaibelii Wierzb. Ex Heuff. Industrial Crops and Products 2019, 139. https://doi.org/10.1016/j.indcrop.2019.111549

Supplementary material for: [https://doi.org/10.1016/j.indcrop.2019.111549]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3296

Chemical Composition of Aster Albanicus Deg. (Asteraceae) Essential Oil: Taxonomical Implications

The composition of essential oil isolated from the areal parts of Aster albanicus Deg, an endemic species of the central Balkans, was analyzed. In total, 111 compounds were identified, representing 98% of the essential oil. The essential oil was dominated by sesquiterpene (69.3%) and monoterpene hydrocarbons (15.9%), with germacrene D as the most abundant compound (34.7%). Several multivariant statistical methods (HCA, NJ, PCoA) were deployed to infer the relation between A. albanicus and other species belonging to this genus. Taxonomical implications are discussed

Phlomis fruticosa l. exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line

Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.This work has been supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (451-03-9/2021-14/200007 and 451-03-68/2022-14/200042). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the institutional scien-tific employment program-contract for M.I. Dias and L. Barros; to FEDER-Interreg España-Portugal program for financial support through the project 0377_Iberphenol_6_E and TRANSCoLAB 0612_TRANS_CO_LAB_2_P.info:eu-repo/semantics/publishedVersio

Thomas Bernhard. Il paradosso del teatro nell'epoca postdrammatica.

Relazione fra Thomas Bernhard ed il teatro nell'epoca postdrammatica, in particolare con attori e registi. Analisi di alcune opere dell'autore (Minetti, Ritter Dene Voss, Claus Peymann kauft sich eine Hose und geht mit mir essen) dove emerge con evidenza l'interesse dell'autore per l'istituzione teatrale e per la performance teatrale