Reactors for microbial electrobiotechnology

From the first electromicrobial experiment to a sophisticated microbial electrochemical process - it all takes place in a reactor. Whereas the reactor design and materials used strongly influence the obtained results, there are no common platforms for MES reactors. This is a critical convention gap, as cross-comparison and benchmarking among MES as well as MES vs. conventional biotechnological processes is needed. Only knowledge driven engineering of MES reactors will pave the way to application and commercialization. In this chapter we first assess the requirements on reactors to be used for bioelectrochemical systems as well as potential losses caused by the reactor design. Subsequently, we compile the main types and designs of reactors used for MES so far, starting from simple H-cells to stirred tank reactors. We conclude with a discussion on the weaknesses and strengths of the existing types of reactors for bioelectrochemical systems that are scored on design criteria and draw conclusions for the future engineering of MES reactors. [GRAPHICS]

Electrobioremediation of oil spills

Annually, thousands of oil spills occur across the globe. As a result, petroleum substances and petrochemical compounds are widespread contaminants causing concern due to their toxicity and recalcitrance. Many remediation strategies have been developed using both physicochemical and biological approaches. Biological strategies are most benign, aiming to enhance microbial metabolic activities by supplying limiting inorganic nutrients, electron acceptors or donors, thus stimulating oxidation or reduction of contaminants. A key issue is controlling the supply of electron donors/acceptors. Bioelectrochemical systems (BES) have emerged, in which an electrical current serves as either electron donor or acceptor for oil spill bioremediation. BES are highly controllable and can possibly also serve as biosensors for real time monitoring of the degradation process. Despite being promising, multiple aspects need to be considered to make BES suitable for field applications including system design, electrode materials, operational parameters, mode of action and radius of influence. The microbiological processes, involved in bioelectrochemical contaminant degradation, are currently not fully understood, particularly in relation to electron transfer mechanisms. Especially in sulfate rich environments, the sulfur cycle appears pivotal during hydrocarbon oxidation. This review provides a comprehensive analysis of the research on bioelectrochemical remediation of oil spills and of the key parameters involved in the process

Ultra-low-energy non-volatile straintronic computing using single multiferroic composites

The primary impediment to continued downscaling of traditional charge-based electronic devices in accordance with Moore\u27s law is the excessive energy dissipation that takes place in the device during switching of bits. One very promising solution is to utilize multiferroicheterostructures, comprised of a single-domain magnetostrictive nanomagnet strain-coupled to a piezoelectric layer, in which the magnetization can be switched between its two stable states while dissipating minuscule amount of energy. However, no efficient and viable means of computing is proposed so far. Here we show that such single multiferroic composites can act as universal logic gates for computing purposes, which we demonstrate by solving the stochastic Landau-Lifshitz-Gilbert equation of magnetization dynamics in the presence of room-temperature thermal fluctuations. The proposed concept can overwhelmingly simplify the design of large-scale circuits and portend a highly dense yet an ultra-low-energy computing paradigm for our future information processing systems

Adaptive Body Area Networks Using Kinematics and Biosignals

The increasing penetration of wearable and implantable devices necessitates energy-efficient and robust ways of connecting them to each other and to the cloud. However, the wireless channel around the human body poses unique challenges such as a high and variable path-loss caused by frequent changes in the relative node positions as well as the surrounding environment. An adaptive wireless body area network (WBAN) scheme is presented that reconfigures the network by learning from body kinematics and biosignals. It has very low overhead since these signals are already captured by the WBAN sensor nodes to support their basic functionality. Periodic channel fluctuations in activities like walking can be exploited by reusing accelerometer data and scheduling packet transmissions at optimal times. Network states can be predicted based on changes in observed biosignals to reconfigure the network parameters in real time. A realistic body channel emulator that evaluates the path-loss for everyday human activities was developed to assess the efficacy of the proposed techniques. Simulation results show up to 41% improvement in packet delivery ratio (PDR) and up to 27% reduction in power consumption by intelligent scheduling at lower transmission power levels. Moreover, experimental results on a custom test-bed demonstrate an average PDR increase of 20% and 18% when using our adaptive EMG- and heart-rate-based transmission power control methods, respectively. The channel emulator and simulation code is made publicly available at https://github.com/a-moin/wban-pathloss.Comment: Accepted for publication in IEEE Journal of Biomedical and Health Informatic

Fabrication and characterization of flexible spray-coated antennas

This paper investigates the potential of using spray coating as a methodology for flexible antenna fabrication. The methodology has advantages compared with other antenna-printing techniques, such as screen-printing and gravure printing (more flexibility in design), or inkjet printing (faster production). The methodology is demonstrated using two different types of folded dipole antennas that are designed to operate in the ultra-high frequency radio-frequency identification (UHF RFID) band. Both antennas show good agreement between simulation and measurement of the spray-coated samples in terms of power reflection coefficient and gain. The two folded dipoles, with and without ground plane, show comparable performance in terms of gain, as similar antennas found in literature. The folded dipole on a ground plane is more stable near conductive surfaces and on the human body. Given these results, we conclude that spray coating is a good technique for printing small to medium sized batches of antennas

An EMG Gesture Recognition System with Flexible High-Density Sensors and Brain-Inspired High-Dimensional Classifier

EMG-based gesture recognition shows promise for human-machine interaction. Systems are often afflicted by signal and electrode variability which degrades performance over time. We present an end-to-end system combating this variability using a large-area, high-density sensor array and a robust classification algorithm. EMG electrodes are fabricated on a flexible substrate and interfaced to a custom wireless device for 64-channel signal acquisition and streaming. We use brain-inspired high-dimensional (HD) computing for processing EMG features in one-shot learning. The HD algorithm is tolerant to noise and electrode misplacement and can quickly learn from few gestures without gradient descent or back-propagation. We achieve an average classification accuracy of 96.64% for five gestures, with only 7% degradation when training and testing across different days. Our system maintains this accuracy when trained with only three trials of gestures; it also demonstrates comparable accuracy with the state-of-the-art when trained with one trial

Chain elongation in anaerobic reactor microbiomes to recover resources from waste

Different microbial pathways can elongate the carbon chains of molecules in open cultures of microbial populations (i.e. reactor microbiomes) under anaerobic conditions. Here, we discuss three such pathways: 1. homoacetogenesis to combine two carbon dioxide molecules into acetate; 2. succinate formation to elongate glycerol with one carbon from carbon dioxide; and 3. reverse β oxidation to elongate short-chain carboxylates with two carbons into medium-chain carboxylates, leading to more energy-dense and insoluble products (e.g. easier to separate from solution). The ability to use reactor microbiomes to treat complex substrates can simultaneously address two pressing issues: 1. providing proper waste management; and 2. producing renewable chemicals and fuels.The authors thank Wolfgang Bucket (MPI Marburg) for assistance with Figure 1. C.M.S. and L.T.A. were supported by the U. S. Army Research Laboratory and the U. S. Army Research Office under contract/grant number W911NF-12-1-0555. H.R. was supported for this work by the Cornell University Agricultural Experiment Station federal formula funds, Project No. NYC-123452 received from the National Institutes for Food and Agriculture (NIFA), U.S. Department of Agriculture. K.R. was supported by the European Research Council Starter Grant Electrotalk and the Multidisciplinary Research Partnership Ghent Bio-Economy. A.J.M.S. was supported by the Chemical Sciences division of the Netherlands Science Foundation (CW-TOP 700.55.343) and the European Research Council (ERC grant 323009)