286 research outputs found
Tissue identification with micro-magnetic resonance imaging in a caprine spinal fusion model
Nonunion is a major complication of spinal
interbody fusion. Currently X-ray and computed tomography
(CT) are used for evaluating the spinal fusion process.
However, both imaging modalities have limitations in
judgment of the early stages of this fusion process, as they
only visualize mineralized bone. Magnetic resonance
imaging (MRI) could be of great value as it is able to discriminate
between different types of tissue. A feasibility
study was performed in nine animals from a goat spinal
fusion study, to evaluate the detection capacity of different
tissues with micro-MRI. In this study bioresorbable polylactic
acid cages were used. Six- and 12-months follow-up
specimens were scanned in a 6.3 T micro-MRI scanner.
After scanning, the specimens were processed for histology.
Different types of tissue as well as the degradable cage
material were identified in the fusion zone and designated as
regions of interest (ROIs). Subsequently, the location of
these ROIs was determined on the corresponding micro-
MRI image, and average signal intensities of every individual
ROI were measured. An excellent match was seen
between the histological sections and micro-MRI images.
The micro-MRI images showed quantifiable differences in
signal intensity between bone with adipose marrow, bone
with hematopoietic marrow, fibrocartilage, fibrous tissue,
and degradable implant material. In time the signal intensity
of bone with adipose marrow, bone with hematopoietic red
marrow, and of fibrous tissue remained relatively constant.
On the other hand, the signal intensity of the degradable
implant material and the fibrocartilage changed significantly
in time, indicating change of structure and
composition. In conclusion, in our model using bioresorbable
cages the MRI provides us with detailed information
about the early fusion process and may therefore, allow
early diagnosis of non-union
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A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit genes uncovered novel components of several ciliary structures, including a protein complex that contains δ-tubulin and ε-tubulin and is required for centriole maintenance. The screen also provides an unbiased tool for classifying ciliopathies and showed that many congenital heart disorders are caused by loss of ciliary signaling. Collectively, our study enables a systematic analysis of ciliary function and of ciliopathies, and also defines a versatile platform for dissecting signaling pathways through CRISPR-based screening
Calibrating Car-Following Models using Trajectory Data: Methodological Study
The car-following behavior of individual drivers in real city traffic is
studied on the basis of (publicly available) trajectory datasets recorded by a
vehicle equipped with an radar sensor. By means of a nonlinear optimization
procedure based on a genetic algorithm, we calibrate the Intelligent Driver
Model and the Velocity Difference Model by minimizing the deviations between
the observed driving dynamics and the simulated trajectory when following the
same leading vehicle. The reliability and robustness of the nonlinear fits are
assessed by applying different optimization criteria, i.e., different measures
for the deviations between two trajectories. The obtained errors are in the
range between~11% and~29% which is consistent with typical error ranges
obtained in previous studies. In addition, we found that the calibrated
parameter values of the Velocity Difference Model strongly depend on the
optimization criterion, while the Intelligent Driver Model is more robust in
this respect. By applying an explicit delay to the model input, we investigated
the influence of a reaction time. Remarkably, we found a negligible influence
of the reaction time indicating that drivers compensate for their reaction time
by anticipation. Furthermore, the parameter sets calibrated to a certain
trajectory are applied to the other trajectories allowing for model validation.
The results indicate that ``intra-driver variability'' rather than
``inter-driver variability'' accounts for a large part of the calibration
errors. The results are used to suggest some criteria towards a benchmarking of
car-following models
Two-way multi-lane traffic model for pedestrians in corridors
We extend the Aw-Rascle macroscopic model of car traffic into a two-way
multi-lane model of pedestrian traffic. Within this model, we propose a
technique for the handling of the congestion constraint, i.e. the fact that the
pedestrian density cannot exceed a maximal density corresponding to contact
between pedestrians. In a first step, we propose a singularly perturbed
pressure relation which models the fact that the pedestrian velocity is
considerably reduced, if not blocked, at congestion. In a second step, we carry
over the singular limit into the model and show that abrupt transitions between
compressible flow (in the uncongested regions) to incompressible flow (in
congested regions) occur. We also investigate the hyperbolicity of the two-way
models and show that they can lose their hyperbolicity in some cases. We study
a diffusive correction of these models and discuss the characteristic time and
length scales of the instability
Experimental study of pedestrian flow through a bottleneck
In this work the results of a bottleneck experiment with pedestrians are
presented in the form of total times, fluxes, specific fluxes, and time gaps. A
main aim was to find the dependence of these values from the bottleneck width.
The results show a linear decline of the specific flux with increasing width as
long as only one person at a time can pass, and a constant value for larger
bottleneck widths. Differences between small (one person at a time) and wide
bottlenecks (two persons at a time) were also found in the distribution of time
gaps.Comment: accepted for publication in J. Stat. Mec
Criterion for traffic phases in single vehicle data and empirical test of a microscopic three-phase traffic theory
A microscopic criterion for distinguishing synchronized flow and wide moving
jam phases in single vehicle data measured at a single freeway location is
presented. Empirical local congested traffic states in single vehicle data
measured on different days are classified into synchronized flow states and
states consisting of synchronized flow and wide moving jam(s). Then empirical
microscopic characteristics for these different local congested traffic states
are studied. Using these characteristics and empirical spatiotemporal
macroscopic traffic phenomena, an empirical test of a microscopic three-phase
traffic flow theory is performed. Simulations show that the microscopic
criterion and macroscopic spatiotemporal objective criteria lead to the same
identification of the synchronized flow and wide moving jam phases in congested
traffic. It is found that microscopic three-phase traffic models can explain
both microscopic and macroscopic empirical congested pattern features. It is
obtained that microscopic distributions for vehicle speed difference as well as
fundamental diagrams and speed correlation functions can depend on the spatial
co-ordinate considerably. It turns out that microscopic optimal velocity (OV)
functions and time headway distributions are not necessarily qualitatively
different, even if local congested traffic states are qualitatively different.
The reason for this is that important spatiotemporal features of congested
traffic patterns are it lost in these as well as in many other macroscopic and
microscopic traffic characteristics, which are widely used as the empirical
basis for a test of traffic flow models, specifically, cellular automata
traffic flow models.Comment: 27 pages, 16 figure
Mouse Model of Alagille Syndrome and Mechanisms of Jagged1 Missense Mutations.
BACKGROUND & AIMS: Alagille syndrome is a genetic disorder characterized by cholestasis, ocular abnormalities, characteristic facial features, heart defects, and vertebral malformations. Most cases are associated with mutations in JAGGED1 (JAG1), which encodes a Notch ligand, although it is not clear how these contribute to disease development. We aimed to develop a mouse model of Alagille syndrome to elucidate these mechanisms. METHODS: Mice with a missense mutation (H268Q) in Jag1 (Jag1+/Ndr mice) were outbred to a C3H/C57bl6 background to generate a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice). Liver tissues were collected at different timepoints during development, analyzed by histology, and liver organoids were cultured and analyzed. We performed transcriptome analysis of Jag1Ndr/Ndr livers and livers from patients with Alagille syndrome, cross-referenced to the Human Protein Atlas, to identify commonly dysregulated pathways and biliary markers. We used species-specific transcriptome separation and ligand-receptor interaction assays to measure Notch signaling and the ability of JAG1Ndr to bind or activate Notch receptors. We studied signaling of JAG1 and JAG1Ndr via NOTCH 1, NOTCH2, and NOTCH3 and resulting gene expression patterns in parental and NOTCH1-expressing C2C12 cell lines. RESULTS: Jag1Ndr/Ndr mice had many features of Alagille syndrome, including eye, heart, and liver defects. Bile duct differentiation, morphogenesis, and function were dysregulated in newborn Jag1Ndr/Ndr mice, with aberrations in cholangiocyte polarity, but these defects improved in adult mice. Jag1Ndr/Ndr liver organoids collapsed in culture, indicating structural instability. Whole-transcriptome sequence analyses of liver tissues from mice and patients with Alagille syndrome identified dysregulated genes encoding proteins enriched at the apical side of cholangiocytes, including CFTR and SLC5A1, as well as reduced expression of IGF1. Exposure of Notch-expressing cells to JAG1Ndr, compared with JAG1, led to hypomorphic Notch signaling, based on transcriptome analysis. JAG1-expressing cells, but not JAG1Ndr-expressing cells, bound soluble Notch1 extracellular domain, quantified by flow cytometry. However, JAG1 and JAG1Ndr cells each bound NOTCH2, and signaling from NOTCH2 signaling was reduced but not completely inhibited, in response to JAG1Ndr compared with JAG1. CONCLUSIONS: In mice, expression of a missense mutant of Jag1 (Jag1Ndr) disrupts bile duct development and recapitulates Alagille syndrome phenotypes in heart, eye, and craniofacial dysmorphology. JAG1Ndr does not bind NOTCH1, but binds NOTCH2, and elicits hypomorphic signaling. This mouse model can be used to study other features of Alagille syndrome and organ development
Low back pain around retirement age and physical occupational exposure during working life
<p>Abstract</p> <p>Background</p> <p>Physical occupational exposure is a risk factor for low back pain in workers but the long term effects of exposure remain unclear. As several countries consider increasing the retirement age, further information on this topic is relevant. This study aimed to describe the prevalence of low back pain among middle aged and aging individuals in the general French population according to physical occupational exposure and retirement status.</p> <p>Methods</p> <p>The study population originated from the French national survey 'Enquête décennale santé 2002'. Low back pain for more than 30 days within the previous twelve months (LBP) was assessed using a French version of the Nordic questionnaire. Occupational exposure was self assessed. Subjects were classified as "exposed" if they were currently or had previously been exposed to handling of heavy loads and/or to tiring postures. The weighted prevalence of LBP was computed separately for men and women, for active (aged 45-59) and retiree (aged 55-74), according to 5-year age group and past/present occupational exposure.</p> <p>Results</p> <p>For active men, the prevalence of LBP was significantly higher in those currently or previously exposed (n = 1051) compared with those never exposed (n = 1183), respectively over 20% versus less than 11%. Among retired men, the prevalence of LBP tended towards equivalence with increasing age among those previously exposed (n = 748) and those unexposed (n = 599).</p> <p>Patterns were quite similar for women with a higher prevalence in exposed active women (n = 741) compared to unexposed (n = 1260): around 25% versus 15%. Similarly, differences between previously exposed (n = 430) and unexposed (n = 489) retired women tended to reduce with age.</p> <p>Conclusion</p> <p>The prevalence of LBP in active workers was associated with occupational exposure. The link with past exposure among retirees decreased with age. These results should be considered for policies dealing with prevention at the workplace and retirement.</p
Evaluation of a multi-disciplinary back pain rehabilitation programme—individual and group perspectives
To evaluate the impact of a multi-disciplinary back pain rehabilitation programme using a combination of individual and group change data. A total of 261 consecutive patients attending an assessment session for the back pain rehabilitation programme completed the SF-36 health survey questionnaire. The patients were requested to complete the questionnaires again at programme completion and at the 6-month follow-up. The Reliable Change Index was used to define 'clinical significance' in terms of the assessment of individual change. Half of those patients considered to be suitable for the programme subsequently completed it. In group terms, non-completers scored lower than completers on all SF-36 scales. Statistically significant improvements were evident for those completing the programme (all scales at P < 0.000), with improvement maintained at follow-up. In individual terms, 'clinical significance' was exceeded most frequently in the Physical Functioning and Role Physical scales. Whilst some participants lost previous improvements between completion and follow-up, others improved over this same time period. The majority of those completing the programme showed improvement in at least one scale. Adding assessment of individual change to traditional group change measures provides greater insight into the impact a rehabilitation programme has upon participants' quality of life. Whilst the programme is clearly effective for those who complete it, work is required to limit post-programme deterioration and improve uptak
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