Pathoblockers or antivirulence drugs as a new option for the treatment of bacterial infections

The rapid development of antimicrobial resistance is threatening mankind to such an extent that the World Health Organization expects more deaths from infections than from cancer in 2050 if current trends continue. To avoid this scenario, new classes of antiinfectives must urgently be developed. Antibiotics with new modes of action are needed, but other concepts are also currently being pursued. Targeting bacterial virulence as a means of blocking pathogenicity is a promising new strategy for disarming pathogens. Furthermore, it is believed that this new approach is less susceptible towards resistance development. In this review, recent examples of anti-infective compounds acting on several types of bacterial targets, e.g., adhesins, toxins and bacterial communication, are described

Characterization of CoRoT target fields with BEST: Identification of periodic variable stars in the IR01 field

We report on observations of the CoRoT IR01 field with the Berlin Exoplanet Search Telescope (BEST). BEST is a small aperture telescope with a wide field of view (FOV). It is dedicated to search for variable stars within the target fields of the CoRoT space mission to aid in minimizing false-alarm rates and identify potential targets for additional science. CoRoT's observational programm started in February 2007 with the "initial run" field (IR01) observed for about two months. BEST observed this field for 12 nights spread over three months in winter 2006. From the total of 30426 stars observed in the IR01 field 3769 were marked as suspected variable stars and 54 from them showed clear periodicity. From these 19 periodic stars are within the part of the CoRoT FOV covered in our data set

Variability survey in the CoRoT SRa01 field: Implications of eclipsing binary distribution on cluster formation in NGC 2264

Time-series photometry of the CoRoT field SRa01 was carried out with the Berlin Exoplanet Search Telescope II (BEST II) in 2008/2009. A total of 1,161 variable stars were detected, of which 241 were previously known and 920 are newly found. Several new, variable young stellar objects have been discovered. The study of the spatial distribution of eclipsing binaries revealed the higher relative frequency of Algols toward the center of the young open cluster NGC 2264. In general Algol frequency obeys an isotropic distribution of their angular momentum vectors, except inside the cluster, where a specific orientation of the inclinations is the case. We suggest that we see the orbital plane of the binaries almost edge-on.Comment: 18 pages, 8 figures, accepted for publication in Ap

The Berlin Exoplanet Search Telescope II. Catalog of Variable Stars. I. Characterization of Three Southern Target Fields

A photometric survey of three Southern target fields with BEST II yielded the detection of 2,406 previously unknown variable stars and an additional 617 stars with suspected variability. This study presents a catalog including their coordinates, magnitudes, light curves, ephemerides, amplitudes, and type of variability. In addition, the variability of 17 known objects is confirmed, thus validating the results. The catalog contains a number of known and new variables that are of interest for further astrophysical investigations, in order to, e.g., search for additional bodies in eclipsing binary systems, or to test stellar interior models. Altogether, 209,070 stars were monitored with BEST II during a total of 128 nights in 2009/2010. The overall variability fraction of 1.2-1.5% in these target fields is well comparable to similar ground-based photometric surveys. Within the main magnitude range of $R\in\left[11,17\right]$, we identify 0.67(3)% of all stars to be eclipsing binaries, which indicates a completeness of about one third for this particular type in comparison to space surveys.Comment: accepted to A

The BAST algorithm for transit detection

The pioneer space mission for photometric exoplanet searches, CoRoT, steadily monitors about 12000 stars in each of its fields of view. Transit detection algorithms are applied to derive promising planetary candidates, which are then followed-up with ground-based observations. We present BAST (Berlin Automatic Search for Transits), a new algorithm for periodic transit detection, and test it on simulated CoRoT data. BAST searches for box-shaped signals in normalized, filtered, variability-fitted, and unfolded light curves. A low-pass filter is applied to remove high-frequency signals, and linear fits to subsections of data are subtracted to remove the star's variability. A search for periodicity is then performed in transit events identified above a given detection threshold. Some criteria are defined to better separate planet candidates from binary stars. From the analysis of simulated CoRoT light curves, we show that the BAST detection performance is similar to that of the Box-fitting Least-Square (BLS) method if the signal-to-noise ratio is high. However, the BAST box search for transits computes 10 times faster than the BLS method. By adding periodic transits to simulated CoRoT data, we show that the minimum periodic depth detectable with BAST is a linearly increasing function of the noise level. For low-noise light curves, the detection limit corresponds to a transit depth d~0.01%, i.e. a planet of 1 Earth radius around a solar-type star.Comment: 4 pages, 3 figures, to be published in A&

Periodic variable stars in CoRoT field LRa02 observed with BEST II

The Berlin Exoplanet Search Telescope II (BEST II) is a small wide field-of-view photometric survey telescope system located at the Observatorio Cerro Armazones, Chile. The high duty cycle combined with excellent observing conditions and millimagnitude photometric precision makes this instrument suitable for ground based support observations for the CoRoT space mission. Photometric data of the CoRoT LRa02 target field collected between November 2008 and March 2009 were analysed for stellar variability. The presented results will help in the future analysis of the CoRoT data, particularly in additional science programs related to variable stars. BEST II observes selected CoRoT target fields ahead of the space mission. The photometric data acquired are searched for stellar variability, periodic variable stars are identified with time series analysis of the obtained stellar light curves. We obtained the light curves of 104335 stars in the CoRoT LRa02 field over 41 nights. Variability was detected in light curves of 3726 stars of which 350 showed a regular period. These stars are, with the exception of 5 previously known variable stars, new discoveries.Comment: The figures with light curves can be find in the A&A journal as online onl

Induction of rare conformation of oligosaccharide by binding to calcium-dependent bacterial lectin: X-ray crystallography and modelling study

Pathogenic micro-organisms utilize protein receptors (lectins) in adhesion to host tissues, a process that in some cases relies on the interaction between lectins and human glycoconjugates. Oligosaccharide epitopes are recognized through their three-dimensional structure and their flexibility is a key issue in specificity. In this paper, we analysed by X-ray crystallography the structures of the LecB lectin from two strains of Pseudomonas aeruginosa in complex with Lewis x oligosaccharide present on cell surfaces of human tissues. An unusual conformation of the glycan was observed in all binding sites with a non-canonical syn orientation of the N-acetyl group of N-acetyl-glucosamine. A PDB-wide search revealed that such an orientation occurs only in 4% of protein/carbohydrate complexes. Theoretical chemistry calculations showed that the observed conformation is unstable in solution but stabilised by the lectin. A reliable description of LecB/Lewis x complex by force field-based methods had proven especially challenging due to the special feature of the binding site, two closely apposed Ca2+ ions which induce strong charge delocalisation. By comparing various force-field parametrisations, we propose a general strategy which will be useful in near future for designing carbohydrate-based ligands (glycodrugs) against other calcium-dependent protein receptors

Pre-discovery observations of CoRoT-1b and CoRoT-2b with the BEST survey

The BEST wide-angle telescope installed at the Observatoire de Haute-Provence and operated in remote control from Berlin by the Institut fuer Planetenforschung, DLR, has observed the CoRoT target fields prior to the mission. The resulting archive of stellar photometric lightcurves is used to search for deep transit events announced during CoRoT's alarm-mode to aid in fast photometric confirmation of these events. The "initial run" field of CoRoT (IRa01) has been observed with BEST in November and December 2006 for 12 nights. The first "long run" field (LRc01) was observed from June to September 2005 for 35 nights. After standard CCD data reduction, aperture photometry has been performed using the ISIS image subtraction method. About 30,000 lightcurves were obtained in each field. Transits of the first detected planets by the CoRoT mission, CoRoT-1b and CoRoT-2b, were found in archived data of the BEST survey and their lightcurves are presented here. Such detections provide useful information at the early stage of the organization of follow-up observations of satellite alarm-mode planet candidates. In addition, no period change was found over ~4 years between the first BEST observation and last available transit observations.Comment: AJ, accepte

Transmit array as a viable 3D printing option for backhaul applications at V-band

Two designs of high gain dielectric lens for a Vband backhaul antenna, compatible with 3D printing, are compared. The available printing materials still have significant losses, which limit the performance of traditional focusing dielectric lenses, as the dome elliptical lens. Herein, we show that an all-dielectric transmit array can present several mechanical and electrical advantages, especially when high gains are required. We demonstrate that even with a compact transmit array (f/d = 067 it is still possible to comply with the usual bandwidth (57-66 GHz) and gain (>30 dBi) requirements for backhaul applications.info:eu-repo/semantics/acceptedVersio

Pulmonary arterial wedge pressure increase during exercise in patients diagnosed with pulmonary arterial or chronic thromboembolic pulmonary hypertension

Background: The course of pulmonary arterial wedge pressure (PAWP) during exercise in patients with pulmonary arterial or chronic thromboembolic pulmonary hypertension (PAH/CTEPH), further abbreviated as pulmonary vascular disease (PVD), is still unknown. The aim of the study was to describe PAWP during exercise in patients with PVD. Methods: In this cross-sectional study, right heart catheter (RHC) data including PAWP, recorded during semi-supine, stepwise cycle exercise in patients with PVD, were analysed retrospectively. We investigated PAWP changes during exercise until end-exercise. Results: In 121 patients (59 female, 66 CTEPH, 55 PAH, 62±17 years) resting PAWP was 10.2±4.1 mmHg. Corresponding peak changes in PAWP during exercise were +2.9 mmHg (95% CI 2.1-3.7 mmHg, p<0.001). Patients ≥50 years had a significantly higher increase in PAWP during exercise compared with those <50 years (p<0.001). The PAWP/cardiac output (CO) slopes were 3.9 WU for all patients, and 1.6 WU for patients <50 years and 4.5 WU for those ≥50 years. Conclusion: In patients with PVD, PAWP increased slightly but significantly with the onset of exercise compared to resting values. The increase in PAWP during exercise was age-dependent, with patients ≥50 years showing a rapid PAWP increase even with minimal exercise. PAWP/CO slopes >2 WU are common in patients with PVD aged ≥50 years without exceeding the PAWP of 25 mmHg during exercise