First documented occurrences of Cladonia krogiana and C. rangiformis in north America

Funding Information: We thank Scott LaGreca for providing a photograph of the specimen of C. rangiformis at BM that was reportedly collected on Bermuda; Bruce Allen and the late Ronald Pursell for allowing us to cite their collections of Homalothecium sericeum from Newfoundland; Zdeněk Palice for enabling us to compare his collection of C. krogiana from the Czech Republic with material from Canada and Norway; Nathalie Djan-Chékar (Provincial Museum of Newfoundland and Labrador) for co-organizing the 2007 Tuckerman Workshop; and Irwin Brodo, James Lendemer, and an anonymous reviewer for their helpful comments on the manuscript. Permission to collect lichens in Fundy National Park and New River Beach Provincial Park (NRBPP) was approved by Renee Wissink (Parks Canada) and Martin MacMullin (NB Department of Tourism, Heritage, and Culture). Fieldwork in NRBPP and follow-up studies were supported in part by the New Brunswick Wildlife Trust Fund and the New Brunswick Environmental Trust Fund. Publisher Copyright: © 2021, New York Botanical Garden. All rights reserved.– Cladonia krogiana, previously known only from Norway and the Czech Republic, is reported here for North America from two localities near the Bay of Fundy, New Brunswick, Canada. It occurs there on open, rocky banks of clear, free-flowing rivers, habitats similar to those in which it has been found in Norway. We also document the occurrence of C. rangiformis in North America, based on collections from two localities on the southwest coast of Conception Bay, on the Avalon Peninsula of the island of Newfoundland, Canada. It is possibly an accidental, but naturalized, introduction in this area, where European settlement began in the early 1600s. A molecular phylogenetic analysis confirmed the identity of one of the Newfoundland specimens. The IGS rDNA haplotype to which it belongs is the same as the most widely distributed haplotype of C. rangiformis in Europe and Macaronesia. Previous reports of C. rangiformis for continental North America are based on misidentifications. A 19th century collection reportedly made on the island of Bermuda, while correctly identified, is of uncertain provenance.Peer reviewe

Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi

DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Re-annotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi

Finding needles in haystacks: Linking scientific names, reference specimens and molecular data for Fungi

DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Reannotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi.B.R. and C.L.S. acknowledge support from the Intramural Research Program of the National Institutes of Health, National Library of MedicinePeer Reviewe

Finding needles in haystacks:Linking scientific names, reference specimens and molecular data for Fungi

DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Reannotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi.The Intramural Research Programs of the National Center for Biotechnology Information, National Library of Medicine and the National Human Genome Research Institute, both at the National Institutes of Health.http://www.ncbi.nlm.nih.gov/bioproject/PRJNA177353am201

Finding needles in haystacks : linking scientific names, reference specimens and molecular data for Fungi

DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Reannotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi.The Intramural Research Programs of the National Center for Biotechnology Information, National Library of Medicine and the National Human Genome Research Institute, both at the National Institutes of Health.http://www.ncbi.nlm.nih.gov/bioproject/PRJNA177353am201

Phylogenetic placement within Lecanoromycetes of lichenicolous fungi associated with Cladonia and some other genera

Though most of the lichenicolous fungi belong to the Ascomycetes, their phylogenetic placement based on molecular data is lacking for numerous species. In this study the phylogenetic placement of 19 species of lichenicolous fungi was determined using four loci (LSU rDNA, SSU rDNA, ITS rDNA and mtSSU). The phylogenetic analyses revealed that the studied lichenicolous fungi are widespread across the phylogeny of Lecanoromycetes. One species is placed in Acarosporales, Sarcogyne sphaerospora; five species in Dactylosporaceae, Dactylospora ahtii, D. deminuta, D. glaucoides, D. parasitica and Dactylospora sp.; four species belong to Lecanorales, Lichenosticta alcicorniaria, Epicladonia simplex, E. stenospora and Scutula epiblastematica. The genus Epicladonia is polyphyletic and the type E. sandstedei belongs to Leotiomycetes. Phaeopyxis punctum and Bachmanniomyces uncialicola form a well supported clade in the Ostropomycetidae. Epigloea soleiformis is related to Arthrorhaphis and Anzina. Four species are placed in Ostropales, Corticifraga peltigerae, Cryptodiscus epicladonia, C. galaninae and C. cladoniicola comb. nov. (= Lettauia cladoniicola). Three new species are described, Dactylospora ahtii, Cryptodiscus epicladonia and C. galaninae.Peer reviewe

Notes on lichenicolous Pleosporales, with two new species, Didymocyrtis azorica and Pseudopyrenidium epipertusariae (Phaeosphaeriaceae).

The identity and phylogenetic placement of some lichenicolous Pleosporales were studied using morphological and molecular data. Two new species are described, Didymocyrtis azorica on Hypotrachyna rockii, from the Azores, and Pseudopyrenidium epipertusariae on Pertusaria pertusa, from Spain in oceanic montane woods, with a Phoma-like asexual state. This is the second species of Pseudopyrenidium, and the morphological similarities with P. tartaricola are discussed. In addition, Evernia prunastri is a new host for Didymocyrtis ramalinae, and Heterodea muelleri is a new host for D. cladoniicola, which is newly reported for Australia

Cladonia subulata y Cladonia rei dos especies conflictivas

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Contribución a la resolución de un viejo problema taxonómico: Cladonia subrangiformis

Objetivos: Cladonia subrangiformis Sandst.es una especie que se desarrolla sobre sustratos básicos o neutros, con distribución mediterránea (Ahti & Shorabi, 2006) y, desde el punto de vista morfológico, está estrechamente relacionada con Cladonia furcata (Huds.) Schrad. que presenta distribución cosmopolita. El rango taxonómico de estas dos especies, así como su independencia ha sido muy discutido. De acuerdo con Sandstede (1922), su principal diferencia es la presencia de atranorina (talos K+ amarillo) en C. subrangiformis, mientras que C. furcata, por lo general, solo presenta ácido fumarprotocetrárico. Por su morfología son muy variables, lo que ha llevado a la descripción de varios táxones infraespecíficos en C. furcata, que se consideran modificaciones fenotípicas (Jahns & Beltman, 1973; Ahti, 1977). Algunos autores consideran que las diferencias entre C. furcata y C. subrangiformis no son suficientes para otorgarles el rango de especie, y debería hablarse de subespecies o variedades (Hawksworth 1969; Wirth 1995; James 2009). Sin embargo, Burgaz & Ahti (2009) aceptan el rango de especie. Así, el objetivo de este trabajo es intentar dilucidar el estatus taxonómico de estas dos especies mediante datos basados en secuencias de ADN. Material y Método: Se estudiaron 805 muestras procedentes de los herbarios CAMB, FH, H, MA-lichen, MACB, L, S y UPS. Se realizó un estudio morfológico siguiendo los criterios de Burgaz & Ahti (2009), de los metabolitos secundarios mediante TLC (White & James, 1985) y un estudio filogenético, en el que se analizaron las secuencias obtenidas de tres loci (ITS rDNA, IGS rDNA y rpb2) por Máxima Parsimonia, Máximo Likelihood e inferencia Bayesiana. Resultados: Los análisis filogenéticos resolvieron dos clados monofiléticos con alto apoyo. Los dos clados agruparon muestras con morfología que correspondía a ambos taxones, así como muestras químicamente diversas. Conclusión: Nuestros resultados indican que ni C. furcata ni C. subrangiformis son monofiléticas; sin embargo, la existencia de dos clados no coincidentes con las categorías taxonómicas establecidas sugiere una especiación críptica. Bibliografía: Ahti, T. 1977. In: M. R. D. Seaward (ed.). Academic Press, London. Ahti, T. & Sohrabi, M. 2006. Flora Mediterranea 16: 139-144. Burgaz, A. R. & Ahti, T. 2009. Flora Liquenológica Ibérica 4: 1-111. Hawksworth, D.L. 1969. Lichenologist 4: 105-193. Jahns, H. M. & Beltman, H. A. 1973. -Lichenologist 5: 349-367. James, P. W. 2009. In: Smith, C. W. et al. (eds.). London: Natural History Museum Publications, London. Sandstede, H. 1922. Abbandl. Naturrw. Ver. Bremen 25: 89-243. Wirth, V. 1995. Verlag Eugen Ulmer, Stuttgart.Peer Reviewe