Triplex addressability as a basis for functional DNA nanostructures

Here, we present the formation of a fully addressable DNA nanostructure that shows the potential to be exploited as, for example, an information storage device based on pH-driven triplex strand formation or nanoscale circuits based on electron transfer, The nanostructure is composed of two adjacent hexagonal unit cells (analogous to naphthalene) in which each of the eleven edges has a unique double-stranded DNA sequence, constructed using novel three-way oligonucleotides. This allows each ten base-pair side, just 3.4 nm in length, to be assigned a specific address according to its sequence. Such constructs are therefore an ideal precursor to a nonrepetitive two-dimensional grid on which the "addresses" are located at a precise and known position. Triplex recognition of these addresses could function as a simple yet efficient means of information storage and retrieval. Future applications that may be envisaged include nanoscale circuits as well as subnanometer precision in nanoparticle templating. Characterization of these precursor nanostructures and their reversible targeting by triplex strand formation is shown here using gel electrophoresis, atomic force microscopy, and fluorescence resonance energy transfer (FRET) measurements. The durability of the system to repeated cycling of pH switching is also confirmed by the FRET studies

The Nexus of Cognitive and Emotional Intelligence: A Study on Student Learning Outcomes for Academic Excellence

This study delves into the dynamic interplay between cognitive and emotional intelligence, revealing their synergistic impact on student learning outcomes. Cognitive intelligence is shown to be a cornerstone of analytical thinking and problem solving, whereas emotional intelligence cultivates essential life skills such as self-awareness, empathy, and effective interpersonal relationships. Employing a mixed-methods approach, this research endeavor combined surveys and observational studies involving 250 students and 40 educators. Standardized assessment tools, including the Wechsler Adult Intelligence Scale (WAIS) for cognitive abilities and the Emotional Intelligence Appraisal (EIA), were used to evaluate participants. The findings indicate a strong positive correlation (r = 0.78, p < 0.01) between cognitive and emotional intelligence, emphasizing their collective influence on academic performance (average GPA increase of 12%) and social adaptability (measured through peer interaction scores). The study's outcomes underscore the imperative need for educational frameworks to incorporate both cognitive and emotional intelligence dimensions. By doing so, educators can foster the development of well-rounded learners equipped with the skills necessary to thrive in an increasingly complex world

Prospective study on traumatic hollow viscus injuries

INTRODUCTION: Trauma is a major health problem in all countries and it unfortunately forms 60-70 % of all emergency admissions to any hospital. Trauma patients need the most important care since these are the patients who can be salvaged to the maximum. Mahatma Gandhi Memorial Government Hospital, Trichy, attached to K.A.P.V Government Medical College is one of the tertiary centre for trauma in Tamilnadu in which majority of patients admitted to surgical triage ward are due to trauma. Systemic evaluation of these patients revealed orthopaedic and neurological injuries to be the most commonly encountered followed by abdominal trauma forming the third rank next to these injuries. In our hospital hollow viscus injuries are the most commonly encountered abdominal injuries followed by the injuries to solid organs (liver, spleen, kidney). Solid organ injuries can be managed by both conservative and surgical management whereas hollow viscus injuries need emergency surgical intervention since delayed intervention carry a worst prognosis. Hence early diagnosis and intervention in these patients will bring a significant change in the final outcome. AIM OF THE STUDY: 1. Complete systemic examination of all trauma patients admitted to triage ward. 2. Identifying patients having or suspected to have abdominal injury. 3. Appropriate investigations for those patients. 4. To analyse mechanism of injury, organs involved and accompanying injuries in traumatic hollow viscus injuries. 5. To analyse the different surgical procedures and finally the post operative outcome. MATERIALS AND METHODS: The study population are those patients admitted in surgical triage ward in Mahatma Gandhi Memorial Government Hospital (M.G.M.H), Trichy with hollow viscus injuries. Inclusion Criteria: 1. All trauma patients with abdomen injury greater than 13 yrs of age. 2. Both Blunt injury and Penetrating injury abdomen included. Exclusion Criteria: 1. Patients with isolated solid organ injuries. 2. Paediatric age group patients (<12 yrs) Mode of Evaluation: 1. Detailed history and complete physical examination of all the trauma patients, 2. Blood investigations.(complete blood count, Renal function tests), 3. Blood grouping and typing, 4. Chest X-ray PA view, 5. X-ray abdomen erect, 6. USG abdomen & pelvis, 7. CT scan abdomen and pelvis, 8. Paracentesis. Study Design: 1. It is a prospective non randomized descriptive study. 2. Duration of study – January 2012 to November 2013. 3. Ethical committee clearance obtained. 4. An informed written consent obtained either from the patient or from their reliable attenders. 5. Collected data were analyzed using statistical methods. OBSERVATION AND RESULTS: Total number of trauma patients admitted to surgical triage ward during the study period – 9660. Patients suspected of having abdominal injury – 2070. Patients who were completely evaluated and found to have abdominal injury – 74. Patients with solid organ injury alone – 26. Patients with hollow viscus injuries – 48. CONCLUSION: From the study conducted on traumatic hollow viscus injuries in Mahatma Gandhi Memorial Government Hospital (M.G.M.H) Trichy, a tertiary trauma care centre we have come to the following conclusions. Traumatic hollow viscus injuries are quiet common when compared to solid organ injuries. The most commonly affected group are the economically productive age group people .There is a definite male preponderance in our study. The most common etiology for the hollow viscus injuries in our setup is the Road traffic accident (RTA). Since pre operative diagnosis is infrequently done in our study hollow viscus injuries are generally recognised late and hence have a poor prognosis compared to solid organ injuries. Most of the hollow viscus injuries are recognized mainly during exploratory laparotomy only. Small intestine particularly the ileum is the most common hollow viscus to be affected. Since isolated ileal perforation is the most common encountered lesion simple debridment of wound edges followed primary closure of the wound carries a good prognosis in both recovery as well as mortality. Mortality was more or less equal to other studies. The commonest cause of death is associated polytrauma, followed by septicaemia owing to delayed referral to our hospital due to the subtle clinical signs seen in these patients or due to misdiagnosis , severe unstable haemodynamic status at the time of presentation leading to delay in surgery for correcting the heamodynamic status ,respiratory infections in ventilator support patients, SIRS (Systemic Inflammatory Response Syndrome) leading to MODS(Multi Organ Dysfunction Syndrome) and eventually death. To reduce the mortality, the recent concept of damage control surgery has to be stressed strongly whose feasibility and the effectiveness in our setup has to be studied further. So, we conclude that with early diagnosis, timely reference, early surgical intervention and intensive post operative care we can definitely save the life of these trauma patients with these relatively rare injuries. Adequate knowledge regarding suspecting intra abdominal injuries and timely reference to a tertiary trauma care centre can definitely bring a marked difference in the prognosis of these patients

Usefulness of Del Nido cardioplegia in adult cardiac surgery.

Del Nido cardioplegia is a type of cardioplegic solution widely used for complex congenital paediatric cardiac surgery. Its use in adult cardiac surgery is extended now. In our institute we are using it for various adult cardiac surgery patients for past one year. In this study 59 patients who underwent cardiac surgery using del Nido cardioplegia for achieving cardiac arrest, on observation it is effective for 60 to 80 minutes in maintaining arrest without spontaneous electrical activity need of frequent dose is less, surgery can be performed without work flow interruption. Recovery after cross clamp is smooth need for DC shock is very less, post-operative inotropic support were minimal and post-operative ECG and ECHO shows no evidence of perioperative ischemia. From our observation we conclude that it can be used safely in adult cardiac surgery patients also according to surgeon’s preference

Awareness of Plagiarism among Student Teachers of Indian Teacher Educational Institutions

Today, the Internet is a rich source of study materials, and Google Scholar offers free access to a large number of scientific articles. There are excellent research publications available in many more databases. Students have the option of easily copying the material. Reusing, paraphrasing, patchwriting, and ghostwriting without citing the original documents are plagiarism. Plagiarism is increasing in academics, particularly in research. This study aims to study the awareness of plagiarism and to analyze the reasons for plagiarism. The study samples are the student teachers in India. For this study, we used a survey method, and the author prepared a questionnaire to collect data from the student teachers. Study respondents are the student teachers of the Regional Institute of Education Mysore; Purposive sampling was used to select samples. The total number of study respondents is 207. The findings of the study revealed that over 70 per cent of respondents mentioned reasons for plagiarism as lack of relevant literature studying, lack of preparation and time, difficulty checking every piece of work, do not know the manual citation style, lack of knowledge about bibliographic management software and also they stated that getting easily available materials on the internet is also a reason for plagiarism. The study recommends that teachers teach or conduct awareness programs on plagiarism, avoiding plagiarism, citation style, and software

Awareness of Plagiarism among Student Teachers of Indian Teacher Educational Institutions

Today, the Internet is a rich source of study materials, and Google Scholar offers free access to a large number of scientific articles. There are excellent research publications available in many more databases. Students have the option of easily copying the material. Reusing, paraphrasing, patchwriting, and ghostwriting without citing the original documents are plagiarism. Plagiarism is increasing in academics, particularly in research. This study aims to study the awareness of plagiarism and to analyze the reasons for plagiarism. The study samples are the student teachers in India. For this study, we used a survey method, and the author prepared a questionnaire to collect data from the student teachers. Study respondents are the student teachers of the Regional Institute of Education Mysore; Purposive sampling was used to select samples. The total number of study respondents is 207. The findings of the study revealed that over 70 per cent of respondents mentioned reasons for plagiarism as lack of relevant literature studying, lack of preparation and time, difficulty checking every piece of work, do not know the manual citation style, lack of knowledge about bibliographic management software and also they stated that getting easily available materials on the internet is also a reason for plagiarism. The study recommends that teachers teach or conduct awareness programs on plagiarism, avoiding plagiarism, citation style, and software

Developing a Relational View of the Organizing Role of Objects: A study of the innovation process in computer games

Innovation processes create distinctive challenges for coordination. Objects are seen as supporting coordination in such settings by enabling the emergent action needed to deal with a dynamic and uncertain process. Thus, previous work has highlighted the role of different types of objects in coordinating the collaborative tasks undertaken by expert groups, either by motivating the creation of new knowledge or through the translation of understanding. Through an empirical study of innovation processes in the computer games sector, our paper adds to this previous work by finding that the relations between objects, and not the objects alone, help to orchestrate multiple collaborative tasks towards a final outcome within temporal and resource constraints. The relational view which emerges from this study shows how such a ‘system of objects’ is able to stabilize coordination of the process while preserving the emergence and autonomy of games developer practices needed to achieve innovation

Watching mesoporous metal films grow during templated electrodeposition with in situ SAXS

In this paper, we monitor the real-time growth of mesoporous platinum during electrodeposition using small-angle X-ray scattering (SAXS). Previously, we have demonstrated that platinum films featuring the ‘single diamond’ (Fd3m) morphology can be produced from ‘double diamond’ (Pn3m) lipid cubic phase templates; the difference in symmetry provides additional scattering signals unique to the metal. Taking advantage of this, we present simultaneous in situ SAXS/electrochemical measurement as the platinum nanostructures grow within the lipid template. This measurement allows us to correlate the nanostructure appearance with the deposition current density and to monitor the evolution of the orientational and lateral ordering of the lipid and platinum during deposition and after template removal. In other periodic metal nanomaterials deposited within any of the normal topology liquid crystal, mesoporous silica or block copolymer templates previously published, the template and emerging metal have the same symmetry, so such a study has not been possible previously

DC UPS for Critical Loads

The usual way to avoid a computer shutdown during a mains failure is to connect an  uninterruptible power supply system (UPS), which can be suitably modified and fitted inside the computer cabinet by the use of proposed methodology known as a dc UPS.  Conventional desk top UPS systems store electricity in form of chemical energy in batteries and when ever mains fail the batteries provide DC power which is then converter to ac by inverter and then fed to the computer in order to save critical data.  Thus power from mains or from UPS is fed to switched mode power supply unit which converts it to dc to  supply the computer motherboard and accessories.  Leaving the case of the computer powered by mains, duing power failure the dc power is unnecessarily converted to ac and then to dc again in SMPS hence high switching losses and heat is produced there by making the entire system very lowe efficient.  In this work it is proposed to directly feed the dc power from the battery to the computer SMPS system by building a UPS arrangement within the computer system.  This not noly saves space, it is highly efficient compared to the conventional ups system, easy to carry, low cost and reliable since there is no change over operation is required/.  A complete design of an ac to dc power supply with an internal dc UPS is presented in this paper. . A prototype has been fully developed and tested as a PC power supply. Thus the result is achieved at full power about 6-8 minutes