Electron Donating Properties of Thioanisole, p-Methylthioanisole & Diphenyl Sulphide

10-1

Pengaruh Kualitas Layanan Terhadap Kepuasan Nasabah dan Gethok Tular pada Bank Syariah di Kota Palu

Tujuan penelitian ini adalah untuk (1) mengetahui pengaruh kualitas layanan terhadap kepuasan nasabah Bank Syariah di Kota Palu, (2) mengetahui dan menganalisis pengaruh kepuasan nasabah terhadap gethok tular pada nasabah Bank Syariah di Kota Palu. Secara keseluruhan, sampel berjumlah 190 nasabah, yang terdiri dari 131 nasabah Bank Muamalat dan 59 nasabah Bank Syariah Mandiri. Metode analisis yang digunakan adalah Structural Equation Modeling (SEM). Hasil peneltian ini mengungkapkan bahwa (1) kualitas layanan berpengaruh secara signifikan dan positif terhadap kepuasan nasabah Bank Syariah di Kota Palu, (2) kepuasan nasabah berpengaruh signifikan dan positif terhadap gethok tular pada Bank Syariah di Kota Palu

Fulminant Wilson’s Disease Managed with Plasmapheresis as a Bridge to Liver Transplant

New-onset jaundice can be a manifestation of multiple pathologic processes including hemolysis, parenchymal liver disease, and cholestasis; the differential diagnosis is broad and requires a systematic approach. We report a case of a patient who presented with jaundice after starting minocycline for the treatment of acne vulgaris and rapidly developed fulminant liver failure found to be due to Wilson’s disease. She also manifested severe Coomb’s negative hemolytic anemia and renal failure secondary to hepatorenal syndrome. As a bridge to liver transplant, she was successfully treated with plasmapheresis to decrease serum copper in addition to hemodialysis for acidosis and hyperkalemia. She was able to receive a liver and made a full recovery. The case highlights the use of plasmapheresis as an adjunctive treatment modality in cases of fulminant liver failure due to Wilson’s disease

Fulminant Wilson\u27s Disease Managed with Plasmapheresis as a Bridge to Liver Transplant

New-onset jaundice can be a manifestation of multiple pathologic processes including hemolysis, parenchymal liver disease, and cholestasis; the differential diagnosis is broad and requires a systematic approach. We report a case of a patient who presented with jaundice after starting minocycline for the treatment of acne vulgaris and rapidly developed fulminant liver failure found to be due to Wilson\u27s disease. She also manifested severe Coomb\u27s negative hemolytic anemia and renal failure secondary to hepatorenal syndrome. As a bridge to liver transplant, she was successfully treated with plasmapheresis to decrease serum copper in addition to hemodialysis for acidosis and hyperkalemia. She was able to receive a liver and made a full recovery. The case highlights the use of plasmapheresis as an adjunctive treatment modality in cases of fulminant liver failure due to Wilson\u27s disease

The alternate GNB3 splice variant, Gβ3s, exhibits an altered signalling response to EGF stimulation, which leads to enhanced cell migration

It has recently been reported that the duplication of the GNB3 gene has been shown to be directly linked to an obesity phenotype, both in humans and also in a humanised mouse model. Moreover, the common human GNB3 c.825C>T polymorphism (rs5443) causes this ubiquitously expressed gene to be aberrantly spliced approximately 50% of the time leading to the production of both a normal Gβ3 protein and a truncated, possibly less stable subunit, known as Gβ3s. The presence of the GNB3 825T allele has previously been shown to be associated with predisposition to hypertension, obesity, various cancers, Alzheimers, age related cognitive function, erectile dysfunction as well as a marker for pharmacogenetic drug action. Great controversy, however, currently exists as to whether these phenotypes associated with the 825T allele are a) mainly due to the presence of the smaller, possibly more active, Gβ3s subunit or b) merely down to the haploinsufficiency of the normal GNB3 transcript, due to its frequent aberrant splicing. In order to try and address these two conflicting hypothesis, we report on the identification and characterisation of signalling alterations unique to the presence of Gβ3s protein subunit. Moreover we also show the physiological consequences associated with altered signalling, directly induced by the Gβ3s subunit. For this, we used both an EBV transformed lymphoblast cell line homozygote for GNB3 825T/825T (TT) and a stable Gβ3s expressing recombinant COS-7 clone. In both of these cell lines that express the Gβ3s subunit, we found enhanced cytosolic calcium influx upon stimulation with EGF, TGFα and VEGF ligands, as compared to “normal” GNB3 controls with the 825C/825C (CC) genotype. This aberrant calcium influx also led to an increase in ERK, but not AKT1, phosphorylation. Despite the lack of AKT1 activation, we paradoxically observed a significant increase in phosphorylation of its downstream substrates, namely mTOR and p70S6k (KS6B2). Moreover we observed a decrease in phospho FoxO3a only in Gβ3s expressing cells, but not in the “normal” GNB3 (CC) control cell line. The presence of the Gβ3s subunit also appeared to alter the distinct localisation patterns of both Foxo3a and AKT1, while also increasing the colocalisation of mTOR and p70S6K. Subsequent growth factor stimulation studies revealed that EGF treatment, of Gβ3s expressing cells, appeared to cause a significant decrease in cAMP levels, which, in turn resulted in both enhanced caveolin-1a phosphorylation, and an increase in actin stress fibre formation. The identification of these distinct Gβ3s specific signalling alterations were indicative of a more aggressive migratory phenotype. This led us to further investigate and confirm that the presence of the Gβ3s subunit also appears to cause significantly enhanced migration and robust scratch wound healing kinetics, as compared to cells harbouring only the normal copy of the gene. These data therefore present convincing evidence that the Gβ3s subunit is stable, functional and its presence can significantly alter signalling pathways, in different cell types

NRF2 regulates HER1 signaling pathway to modulate the sensitivity of ovarian cancer cells to lapatinib and erlotinib

NF-E2-related factor 2 (NRF2) regulates the transcription of a battery of metabolic and cytoprotective genes. NRF2 and epidermal growth factor receptors (EGFRs/HERs) are regulators of cellular proliferation and determinants of cancer initiation and progression. NRF2 and HERs confer cancers with resistance to several therapeutic agents. Nevertheless, there is limited understanding of the regulation of HER expression and activation and the link between NRF2 and HER signalling pathways. We show that NRF2 regulates both basal and inducible expression of HER1, as treatment of ovarian cancer cells (PEO1, OVCAR3, and SKOV3) with NRF2 activator tBHQ inducing HER1, while inhibition of NRF2 by siRNA knockdown or with retinoid represses HER1. Furthermore, treatment of cells with tBHQ increased total and phosphorylated NRF2, HER1, and AKT levels and compromised the cytotoxic effect of lapatinib or erlotinib. Treatment with siRNA or retinoid antagonised the effect of tBHQ on NRF2 and HER1 levels and enhanced the sensitivity of ovarian cancer cells to lapatinib or erlotinib. Pharmacological or genetic inhibition of NRF2 and/or treatment with lapatinib or erlotinib elevated cellular ROS and depleted glutathione. This extends the understanding of NRF2 and its regulation of HER family receptors and opens a strategic target for improving cancer therapy

Computer recommendations for an automatic approach and landing system for V/STOL aircraft. Volume 1 - Computer recommendations

Evaluation of digital computer for V/STOL aircraft automatic approach and landing syste

Perbaikan Beban Kerja Fisik Dan Mental Pada Pembuatan Keripik Singkong Menggunakan Quick Exposure Check Dan National Aeronautics and Space Administration - Task Load Index (Studi Kasus: Ud. Lumba-lumba, Kecamatan Turen, Kabupaten Malang)

Lingkungan kerja mempunyai banyak faktor yang berkontribusi pada efisiensi dan efektivitas lingkungan kerja tersebut. Faktor manusia perlu untuk dipertimbangkan karena manusia memiliki keterbatasan dalam segi fisik dan mental. Pada penelitian ini, penilaian telah dilakukan untuk mengetahui besar beban kerja yang diderita oleh pekerja dan memberikan perbaikan untuk menurunkan beban kerja tersebut. Quick Exposure Check (QEC) digunakan sebagai penilaian terhadap exposure dari Musculoskeletal Disorders (MSDs) yang menggambarkan beban kerja fisik. Sedangkan Beban kerja mental dinilai menggunakan National Aeronautics and Space Administration - Task Load Index (NASA-TLX). Penilaian QEC menunjukkan 4 dari 8 set skor dikategorikan exposure tinggi. Penilaian NASA-TLX juga menunjukkan bahwa pekerja pada proses penggorengan menderita beban mental yang tinggi pula dengan nilai rata-rata 72.20833. Beban kerja yang tinggi ini disebabkan banyak faktor diantaranya adalah tata letak workstation tidak diatur dengan ergonomis. Pekerja juga menggunakan alat yang tidak ergonomis. Manajemen pabrik juga tidak mengatur jam kerja dengan baik. Pekerja dapat bekerja hingga 11 jam/hari dan 5 jam tanpa istirahat. Bahan baku juga tidak dikontrol dengan baik sehingga bahan baku tidak datang dengan teratur dan konstan