Efficiency of Rz-1 based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Geece

A survey was carried out to investigate the current situation concerning rhizomania disease incidence in sugar beet cultivation of Greece. A systematic field evaluation over locations and years revealed a consistent disease severity pattern according to favourable agroclimatic conditions and pointed to the so far effectiveness of the Rz1 gene-based resistance, as no major disease outbreaks were observed. Molecular analyses aiming at the characterization of the type and genetic diversity of the virus further confirmed the widespread occurrence of BNYVV in the country, as evidenced by RT-PCR amplification of all five known genomic molecules and nested-PCR assays. None of the isolates contained an RNA 5, typically found in pathotype P. On the basis of RFLP patterns, all BNYVV isolates analysed were classified as pathotype A. Sequence determination of the full-length RNA 3-encoded p25 protein, responsible for symptom development, revealed amino acid motifs ACHG/VCHG in the hypervariable region aa67–70. The presence of valine in position 67 did not appear associated with increased pathogenicity and resistance breaking properties, as earlier reported

Physical self efficacy, injury, history and locus of control as predictors of athletes\u27 expected recovery actions in response to various levels of injury severity

Athletes and coaches from a university athletic program at the highest level of competition in the National Collegiate Athletic Association and a college athletic program at the lowest level of competition participated in this project designed to study athletes expected recovery actions when presented with various levels of injury. Physical self-efficacy, locus of control and injury history were considered as mediators in the expected recovery processes including expected recovery time, the number of recovery strategies, and reaction to permission for competition after injury. The Physical Self-Efficacy Scale (Ryckman, Robbins, Thorton, & Cantrell, 1982) and the Nowicki-Strickland Locus of Control Scale for Children (Nowicki & Strickland, 1973, adapted for use with college students and adults), were determined to be related to an athlete\u27s predicted recovery actions from specific mild, moderate, and severe injury. Coaches were determined to have the ability to accurately categorize athletes regarding physical self-efficacy and locus of control. Athletes with an internal locus of control were found to predict different recovery actions from athletes with an external locus of control. Likewise, athletes with high physical self-efficacy were found to predict different recovery actions from athletes with a low physical self-efficacy. When athletes, who were classified as having both an internal locus of control and a high physical self-efficacy were compared to athletes classified as having both external locus of control and low self-efficacy, additional differences were observed. The athletes differed on ratings of their own ability, predictions of recovery times, and on level of awareness of the injury site after approval for competition. Repeated injuries were determined to increase extemality of locus of control of athletes. Previous experience with injury, the athlete\u27s perceived level of ability in sport, and the coach\u27s perceptions of the athlete\u27s response to injury were also considered for their predictive ability for injury recovery

Intelligent Tutoring Systems for Generation Z's Addiction

As generation Z's big data is flooding the Internet through social nets, neural network based data processing is turning an important cornerstone, showing significant potential for fast extraction of data patterns. Online course delivery and associated tutoring are transforming into customizable, on-demand services driven by the learner. Besides automated grading, strong potential exists for the development and deployment of next generation intelligent tutoring software agents. Self-adaptive, online tutoring agents exhibiting "intelligent-like" behavior, being capable "to learn" from the learner, will become the next educational superstars. Over the past decade, computer-based tutoring agents were deployed in a variety of extended reality environments, from patient rehabilitation to psychological trauma healing. Most of these agents are driven by a set of conditional control statements and a large answers/questions pairs dataset. This article provides a brief introduction on Generation Z's addiction to digital information, highlights important efforts for the development of intelligent dialogue systems, and explains the main components and important design decisions for Intelligent Tutoring System.Comment: 4 page

Identificação e clonagem de promotores raiz-específicos de eucalipto.

A utilização de promotores tecido - específicos é uma das principais estratégias para direcionar a expres são de transgenes a determinados órgãos ou tipos celulares . Por isso, o objetivo deste trabalho foi a clonagem de promotores raiz - específicos , a partir de dados de RNA - Seq obtidos de bibliotecas de raiz, xilema e folha de eucalipto. Dois genes com padrão d e expressão preferencial em raiz foram inicialmente selecionados para isolamento da região promotora. Fragmentos de 1kb foram clonados em vetor binário pCAMBIA2301, fusionados ao gene repórter da beta - glucuronidase (GUS) , para transformação de Populus trem ula x Populus alba via Agrobacterium tumefaciens .Edição dos anais do 3º Simpósio de Bioquímica e Biotecnologia, 2013. Londrina

Para repensar o ensino de genética : levantamento e análise da produção acadêmica da área do ensino de ciências e Biologia no Brasil

Buscou-se analisar a produção científica envolvendo a temática Ensino de Genética e Afins, provindas dos três principais eventos acadêmicos da Área de Ensino de Biologia e Ciências (EPEB, ENPEC, EREBIO/ENEBIO), entre 2001 e 2007. Foram identificados 152 trabalhos, em 13 anais, os quais foram categorizados em duas modalidades (Pesquisa acadêmica e Proposição de atividades-práticas), e em diversos focos e estratégias. Realizou-se, adicionalmente, um levantamento de dissertação e teses sobre a temática (52 títulos), a partir de bancos digitalizados presentes na Internet e sítios de Programas de Pós-Graduação em Ensino de Ciências e em Educação. Sob a luz deste trabalho, apontam-se considerações finais sobre os desafios presentes na renovação da genética escolar, ao se reconhecer a necessidade de superação do ensino fragmentado, desatualizado e descontextualizado

Indução de calogênese in vitro em folhas de erva-mate (Ilex paraguariensis).

Resumo