Characterisation and neutralisation of Aeromonas hydrophila enterotoxin in the rabbit ileal-loop model

Cell-free culture filtrates and crude enterotoxin preparations from six strains of Aeromonas hydrophiza caused the accumulation of fluid in rabbit ileal loops. This activity was due to a non-dialysable, heat and acid-labile antigenic protein and was lost when culture filtrates and crude enterotoxin preparations were heated at 60°C for 20 min. or 56°C for 30 min. respectively. Maximum activity was observed at pH 8.0-10.0; there was a gradual loss at lower pH and activity was abolished in culture filtrates held at pH 3.0 and crude enterotoxin preparations held at pH 4.0. Titration of the crude enterotoxin preparations in rabbit ileal loops showed that the ED50 (equivalent to 1 unit of toxin) was contained in 25 μg of protein; a logarithmic plot of the neutralisation coefficients against antiserum concentrations showed that one unit of antitoxin was contained in 42×10−4 ml of the antiserum

Recovering Grammar Relationships for the Java Language Specification

Grammar convergence is a method that helps discovering relationships between different grammars of the same language or different language versions. The key element of the method is the operational, transformation-based representation of those relationships. Given input grammars for convergence, they are transformed until they are structurally equal. The transformations are composed from primitive operators; properties of these operators and the composed chains provide quantitative and qualitative insight into the relationships between the grammars at hand. We describe a refined method for grammar convergence, and we use it in a major study, where we recover the relationships between all the grammars that occur in the different versions of the Java Language Specification (JLS). The relationships are represented as grammar transformation chains that capture all accidental or intended differences between the JLS grammars. This method is mechanized and driven by nominal and structural differences between pairs of grammars that are subject to asymmetric, binary convergence steps. We present the underlying operator suite for grammar transformation in detail, and we illustrate the suite with many examples of transformations on the JLS grammars. We also describe the extraction effort, which was needed to make the JLS grammars amenable to automated processing. We include substantial metadata about the convergence process for the JLS so that the effort becomes reproducible and transparent

Hepatitis Associated with a Sarcocystis canis–like Protozoan in a Hawaiian Monk Seal (Monachus schauinslandi)

A Hawaiian monk seal (Monachus schauinslandi) died in captivity at the National Marine Fisheries Service, Kewalo Basin Facility in Honolulu, Hawaii. The animal was icteric, and the liver was friable. Microscopic lesions were detected in the colon and liver. Colonic lesions included multifocal, necrohemorrhagic colitis associated with gram-negative bacilli. The liver lesions included random hepatic necrosis and cholestasis. Asexual stages of a Sarcocystis canis–like apicomplexan were detected in hepatocytes. The parasite divided by endopolygeny. Merozoites occasionally formed rosettes around a central residual body. Ultrastructurally, merozoites lacked rhoptries. This is the first report of S. canis infection in M. schauinslandi, which is an endangered pinniped in U.S. waters

Agility in humanitarian supply chain: an organizational information processing perspective and relational view

Humanitarian organizations are increasingly facing challenges in terms of improving the efficiency and the effectiveness of their disaster relief efforts. These challenges often arise due to a lack of trust, poor collaboration and an inability to respond to disaster affected areas in a timely manner. Our study attempts to understand how these challenges are overcome by seeking answers to questions related to the topics of swift-trust, collaboration and agility in humanitarian supply chains. For instance, in our study we have attempted to examine how information sharing and supply chain visibility in humanitarian supply chains improve the swift-trust among the humanitarian actors engaged in disaster relief operations. Further, we attempt to understand how-swift trust, commitment and collaboration among the humanitarian actors improve the agility in humanitarian supply chains. In our study we provide both theoretical and data-driven answers to our stated research gaps. Our theoretical model is firmly grounded in organizational information process theory and relational view. We tested our research hypotheses using variance based structural equation modelling with survey data collected using a web based pre-tested instrument from 147 NGOs respondents drawn from the National Disaster Management Authority database. Our results help to advance the theoretical debates surrounding “swift-trust”, “collaboration” and “agility” in humanitarian settings. We further provide direction to managers engaged in disaster relief operations. The humanitarian actors engaged in disaster relief often fail to understand how to build swift-trust. Moreover, how swift-trust further affects commitment and collaboration which in turn further affect agility in humanitarian supply chains. Thus humanitarian organizations must understand how information sharing and supply chain visibility is key to swift-trust among humanitarian actors and agility in humanitarian supply chains. Finally, we outline the limitations of our study and offer some future research directions for investigation

Polyethylene Maleate Copolyesters as Coating Materials for Piezoelectric Quartz Crystal-based Chemical Sensors

Polyethylene maleate (PEM) was synthesised by direct polycondensation of maleic anhydride and ethylene glycol in toluene under reflux usingp-toluene sulphonic acid as a catalyst. Structure of PEM was further modified by varying nature of diols and acid components, chain length of glycols, incorporation of aromatic and fluorine groups in the chain. Formation of PEM was monitored by gas chromatography. The parameters like degree of polymerisation, number average molecular weight (Mn), and weight average molecular weight (Mw) were calculated from the extent of reaction and stoichiometric ratio of the reactants. The number average molecular weight was also determined using Knauer vapour pressure osmometer. Cohesive energy, volume, and solubility parameters of PEM-based copolyesters were calculated by group contribution method. These PEM-based copolyesters’ have been evaluated as sorbent-coating materials for the detection of organ0 phosphorus(OP)compo using dimethylmethylphosphonate (DMMP) as model compound and piezoelectric crystal detector. PEMbisphenol A is found to be the most sensitive and potential coating material for the detection of OP compounds using piezoelectric crystal detector. Potential PEM-based copolyesters have been characterised by viscosity, infrared spectroscopy, NMR spectroscopy, Mn and thermal stability

Extreme daily rainfall in Pakistan and north India: scale-interactions, mechanisms, and precursors

While much of India is used to heavy precipitation and frequent low-pressure systems during the summer monsoon, towards the northwest and into Pakistan, such events are uncommon. Here, as much as a third of the annual rainfall is delivered sporadically during the winter monsoon by western disturbances. Such events of sparse but heavy precipitation in this region of typically mountainous valleys in the north and desert in the south can be catastrophic, as in the case of the Pakistan floods of July 2010. In this study, we identify extreme precipitation events (EPEs) in a box approximately covering this region (65◦ -78◦W, 25◦ -38◦N) using the APHRODITE gauge-based precipitation product. The role of the large-scale circulation in causing EPEs is investigated: it is found that, during winter, they often coexist with an upper-tropospheric Rossby wave train that has prominent anomalous southerlies over the region of interest. These winter EPEs are also found to be strongly colocated with incident western disturbances whereas those occurring during the summer are found to have a less direct relationship. Conversely, summer EPEs are found to have a strong relationship with tropical lows. A detailed Lagrangian method is used to explore possible sources of moisture for such events, and suggests that in winter, the moisture is mostly drawn from the Arabian Sea, whereas during the summer, it comes from along the African coast and the Indian monsoon trough region

Inverse problem of photoelastic fringe mapping using neural networks

This paper presents an enhanced technique for inverse analysis of photoelastic fringes using neural networks to determine the applied load. The technique may be useful in whole-field analysis of photoelastic images obtained due to external loading, which may find application in a variety of specialized areas including robotics and biomedical engineering. The presented technique is easy to implement, does not require much computation and can cope well within slight experimental variations. The technique requires image acquisition, filtering and data extraction, which is then fed to the neural network to provide load as output. This technique can be efficiently implemented for determining the applied load in applications where repeated loading is one of the main considerations. The results presented in this paper demonstrate the novelty of this technique to solve the inverse problem from direct image data. It has been shown that the presented technique offers better result for the inverse photoelastic problems than previously published works

Optimization of DNA extraction for ISSR studies in Tectona grandis L.f. - an important forest tree species

Four extraction methods and sample types were evaluated for yield, quality and suitability of genomic DNA for ISSR marker amplification in teak (Tectona grandis). Both CTAB and SDS based extraction procedures extracted better quantity and purity index of genomic DNA. Leaves of trees, bud grafts andseedlings yielded better DNA than seeds. Using identical PCR conditions, DNA extraction methods and sample types influenced amplifications of ISSR markers, with no amplifications among seed samples. DNA extraction method and sample type are very important consideration for reproducible ISSR-based molecular marker analysis in tea