101 research outputs found
DQ white-dwarf stars with low C abundance: Possible progenitors
The present paper focuses on the evolution of hydrogen-deficient white dwarfs
with the aim of exploring the consequences of different initial envelope
structures on the carbon abundances expected in helium-rich,
carbon-contaminated DQ white dwarfs. In particular, the evolutionary link
between the DQs with low detected carbon abundances and the PG1159, extreme
horizontal branch, and helium-rich R Coronae Borealis (RCrB) stars is explored.
We present full evolutionary calculations that take a self-consistent treatment
of element diffusion into account as well as expectations for the outer layer
chemical stratification of progenitor stars upon entering the white dwarf
regime. We find that PG1159 stars cannot be related to any DQ white dwarfs with
low C abundances. Instead, we suggest that the latter could constitute the
progeny of the giant, helium-rich RCrB stars.Comment: 10 pages, 10 figures. Accepted for publication in Astronomy and
Astrophysic
Lipid-free Antigen B subunits from echinococcus granulosus: oligomerization, ligand binding, and membrane interaction properties
Background:
The hydatid disease parasite Echinococcus granulosus has a restricted lipid metabolism, and needs to harvest essential lipids from the host. Antigen B (EgAgB), an abundant lipoprotein of the larval stage (hydatid cyst), is thought to be important in lipid storage and transport. It contains a wide variety of lipid classes, from highly hydrophobic compounds to phospholipids. Its protein component belongs to the cestode-specific Hydrophobic Ligand Binding Protein family, which includes five 8-kDa isoforms encoded by a multigene family (EgAgB1-EgAgB5). How lipid and protein components are assembled into EgAgB particles remains unknown. EgAgB apolipoproteins self-associate into large oligomers, but the functional contribution of lipids to oligomerization is uncertain. Furthermore, binding of fatty acids to some EgAgB subunits has been reported, but their ability to bind other lipids and transfer them to acceptor membranes has not been studied.<p></p>
Methodology/Principal Findings:
Lipid-free EgAgB subunits obtained by reverse-phase HPLC were used to analyse their oligomerization, ligand binding and membrane interaction properties. Size exclusion chromatography and cross-linking experiments showed that EgAgB8/2 and EgAgB8/3 can self-associate, suggesting that lipids are not required for oligomerization. Furthermore, using fluorescent probes, both subunits were found to bind fatty acids, but not cholesterol analogues. Analysis of fatty acid transfer to phospholipid vesicles demonstrated that EgAgB8/2 and EgAgB8/3 are potentially capable of transferring fatty acids to membranes, and that the efficiency of transfer is dependent on the surface charge of the vesicles.<p></p>
Conclusions/Significance:
We show that EgAgB apolipoproteins can oligomerize in the absence of lipids, and can bind and transfer fatty acids to phospholipid membranes. Since imported fatty acids are essential for Echinococcus granulosus, these findings provide a mechanism whereby EgAgB could engage in lipid acquisition and/or transport between parasite tissues. These results may therefore indicate vulnerabilities open to targeting by new types of drugs for hydatidosis therapy.<p></p>
Gravitational settling of 22Ne and white dwarf evolution
We study the effects of the sedimentation of the trace element 22Ne in the
cooling of white dwarfs. In contrast with previous studies, which adopted a
simplified treatment of the effects of 22Ne sedimentation, this is done
self-consistently for the first time, using an up-to-date stellar evolutionary
code in which the diffusion equation is coupled with the full set of equations
of stellar evolution. Due the large neutron excess of 22Ne, this isotope
rapidly sediments in the interior of the white dwarf. Although we explore a
wide range of parameters, we find that using the most reasonable assumptions
concerning the diffusion coefficient and the physical state of the white dwarf
interior the delay introduced by the ensuing chemical differentation is minor
for a typical 0.6 Msun white dwarf. For more massive white dwarfs, say M_Wd
about 1.0 Msun, the delay turns out to be considerably larger. These results
are in qualitatively good accord with those obtained in previous studies, but
we find that the magnitude of the delay introduced by 22Ne sedimentation was
underestimated by a factor of about 2. We also perform a preliminary study of
the impact of 22Ne sedimentation on the white dwarf luminosity function.
Finally, we hypothesize as well on the possibility of detecting the
sedimentation of 22Ne using pulsating white dwarfs in the appropriate effective
temperature range with accurately determined rates of change of the observed
periods.Comment: To apper in The Astrophysical Journa
Abell 43: Longest period Planetary Nebula Nucleus variable
Based on 24h high speed photometry of the hybrid PG 1159 star Abell 43, we
have detected 6 sighificant pulsations with periods between 2380 s and 6075 s.
A short (4h) run on the almost spectroscopic twin NGC 7094 central star
resulted in detection of 3 low amplitude pulsations with periods between 2000 s
and 5000 s. The results are close to predictions for g-mode pulsations driven
by the kappa-mechanism induced by the partial ionization of carbon and oxygen.Comment: 5 pages, 5 figures, to be published in Astronomy and Astrophysic
Diversity in the structures and ligand binding sites of nematode fatty acid and retinol binding proteins revealed by Na-FAR-1 from Necator americanus
Fatty acid and retinol binding proteins (FARs) comprise a family of unusual α-helix rich lipid binding proteins found exclusively in nematodes. They are secreted into host tissues by parasites of plants, animals and humans. The structure of a FAR protein from the free-living nematode Caenorhabditis elegans is available, but this protein (Ce-FAR-7) is from a subfamily of FARs that does not appear to be important at the host-parasite interface. We have therefore examined Na-FAR-1 from the blood-feeding intestinal parasite of humans, Necator americanus . The three dimensional structure of Na-FAR-1 in its ligand-free and ligand-bound forms, determined by nuclear magnetic resonance spectroscopy (NMR) and X-ray crystallography, respectively, reveals an a-helical fold similar to Ce-FAR-7, but Na-FAR-1 possesses a larger and more complex internal ligand binding cavity and an additional C-terminal a-helix. Titration of apo -Na-FAR-1 with oleic acid, analysed by NMR chemical shift perturbation, reveals that at least four distinct protein:ligand complexes can be formed. Na-FAR-1, and possibly other FARs, may have a wider repertoire for hydrophobic ligand binding, as confirmed here by our finding that a range of neutral and polar lipids co-purify with the bacterial recombinant protein. Finally, we show by immunohistochemistry that Na-FAR-1 is present in adult worms with a tissue distribution indicative of possible roles in nutrient acquisition by the parasite and in reproduction in the male
Study of sdO models: mode trapping
We present the first description of mode trapping for sdO models. Mode
trapping of gravity modes caused by the He/H chemical transition is found for a
particular model, providing a selection effect for high radial order trapped
modes. Low- and intermediate-radial order {\em p}-modes (mixed modes with a
majority of nodes in the P-mode region) are found to be trapped by the C-O/He
transition, but with no significant effects on the driving. This region seems
to have also a subtle effect on the trapping of low radial order {\em g}-modes
(mixed modes with a majority of nodes in the G-mode region), but again with no
effect on the driving. We found that for mode trapping to have an influence on
the driving of sdO modes (1) the mode should be trapped in a way that the
amplitude of the eigenfunctions is lower in a damping region and (2) in this
damping region significant energy interchange has to be produced.Comment: 10 pages, 13 figures, accepted for publication in MNRAS, 2009
December 1
Fatty acid transfer from Yarrowia lipolytica sterol carrier protein 2 to phospholipid membranes
Sterol carrier protein 2 (SCP2) is an intracellular protein domain found in all forms of life. It was originally identified as a sterol transfer protein, but was recently shown to also bind phospholipids, fatty acids, and fatty-acyl-CoA with high affinity. Based on studies carried out in higher eukaryotes, it is believed that SCP2 targets its ligands to compartmentalized intracellular pools and participates in lipid traffic, signaling, and metabolism. However, the biological functions of SCP2 are incompletely characterized and may be different in microorganisms. Herein, we demonstrate the preferential localization of SCP2 of Yarrowia lipolytica (YLSCP2) in peroxisome-enriched fractions and examine the rate and mechanism of transfer of anthroyloxy fatty acid from YLSCP2 to a variety of phospholipid membranes using a fluorescence resonance energy transfer assay. The results show that fatty acids are transferred by a collision-mediated mechanism, and that negative charges on the membrane surface are important for establishing a "collisional complex". Phospholipids, which are major constituents of peroxisome and mitochondria, induce special effects on the rates of transfer. In conclusion, YLSCP2 may function as a fatty acid transporter with some degree of specificity, and probably diverts fatty acids to the peroxisomal metabolism.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicada
Outer boundary conditions for evolving cool white dwarfs
White dwarf evolution is essentially a gravothermal cooling process,
which,for cool white dwarfs, sensitively depends on the treatment of the outer
boundary conditions. We provide detailed outer boundary conditions appropriate
for computing the evolution of cool white dwarfs employing detailed non-gray
model atmospheres for pure H composition. We also explore the impact on the
white dwarf cooling times of different assumptions for energy transfer in the
atmosphere of cool white dwarfs. Detailed non-gray model atmospheres are
computed taken into account non-ideal effects in the gas equation of state and
chemical equilibrium, collision-induced absorption from molecules, and the
Lyman alpha quasi-molecular opacity. Our results show that the use of detailed
outer boundary conditions becomes relevant for effective temperatures lower
than 5800 and 6100K for sequences with 0.60 and 0.90 M_sun, respectively.
Detailed model atmospheres predict ages that are up to approx 10% shorter at
log L/L_sun=-4 when compared with the ages derived using Eddington-like
approximations at tau_Ross=2/3. We also analyze the effects of various
assumptions and physical processes of relevance in the calculation of outer
boundary conditions. In particular, we find that the Ly_alpha red wing
absorption does not affect substantially the evolution of white dwarfs. White
dwarf cooling timescales are sensitive to the surface boundary conditions for
T_eff < 6000K. Interestingly enough, non-gray effects have little consequences
on these cooling times at observable luminosities. In fact, collision-induced
absorption processes, which significantly affect the spectra and colors of old
white dwarfs with hydrogen-rich atmospheres, have not noticeable effects in
their cooling rates, except throughout the Rosseland mean opacity.Comment: 6 pages, 9 figures, to be published in Astronomy and Astrophysic
Evolutionary and pulsational properties of white dwarf stars
Abridged. White dwarf stars are the final evolutionary stage of the vast
majority of stars, including our Sun. The study of white dwarfs has potential
applications to different fields of astrophysics. In particular, they can be
used as independent reliable cosmic clocks, and can also provide valuable
information about the fundamental parameters of a wide variety of stellar
populations, like our Galaxy and open and globular clusters. In addition, the
high densities and temperatures characterizing white dwarfs allow to use these
stars as cosmic laboratories for studying physical processes under extreme
conditions that cannot be achieved in terrestrial laboratories. They can be
used to constrain fundamental properties of elementary particles such as axions
and neutrinos, and to study problems related to the variation of fundamental
constants.
In this work, we review the essentials of the physics of white dwarf stars.
Special emphasis is placed on the physical processes that lead to the formation
of white dwarfs as well as on the different energy sources and processes
responsible for chemical abundance changes that occur along their evolution.
Moreover, in the course of their lives, white dwarfs cross different
pulsational instability strips. The existence of these instability strips
provides astronomers with an unique opportunity to peer into their internal
structure that would otherwise remain hidden from observers. We will show that
this allows to measure with unprecedented precision the stellar masses and to
infer their envelope thicknesses, to probe the core chemical stratification,
and to detect rotation rates and magnetic fields. Consequently, in this work,
we also review the pulsational properties of white dwarfs and the most recent
applications of white dwarf asteroseismology.Comment: 85 pages, 28 figures. To be published in The Astronomy and
Astrophysics Revie
Liver fatty acid-binding protein binds monoacylglycerol in vitro and in mouse liver cytosol
Liver fatty acid-binding protein (LFABP; FABP1) is expressed both in liver and intestinal mucosa. Mice null for LFABP were recently shown to have altered metabolism of not only fatty acids but also monoacylglycerol, the two major products of dietary triacylglycerol hydrolysis (Lagakos, W. S., Gajda, A. M., Agellon, L., Binas, B., Choi, V., Mandap, B., Russnak, T., Zhou, Y. X., and Storch, J. (2011) Am. J. Physiol. Gastrointest. Liver Physiol. 300, G803-G814). Nevertheless, the binding and transport of monoacylglycerol (MG) by LFABP are uncertain, with conflicting reports in the literature as to whether this single chain amphiphile is in fact bound by LFABP. In the present studies, gel filtration chromatography of liver cytosol from LFABP-/- mice shows the absence of the low molecular weight peak of radiolabeled monoolein present in the fractions that contain LFABP in cytosol from wild type mice, indicating that LFABP binds sn-2 MG in vivo. Furthermore, solution-state NMRspectroscopy demonstrates two molecules of sn-2 monoolein bound in the LFABP binding pocket in positions similar to those found for oleate binding. Equilibrium binding affinities are ~2-fold lower for MG compared with fatty acid. Finally, kinetic studies examining the transfer of a fluorescent MG analog show that the rate of transfer of MG is 7-fold faster from LFABP to phospholipid membranes than from membranes to membranes and occurs by an aqueous diffusion mechanism. These results provide strong support for monoacylglycerol as a physiological ligand for LFABP and further suggest that LFABP functions in the efficient intracellular transport of MG.Instituto de Investigaciones Bioquímicas de La Plat
- …