Classificação de cultivares de brássicas com relação à resistência à traça-das-crucíferas e à presença de glucosinolatos.

O objetivo deste trabalho foi avaliar o efeito de cultivares de repolho e de couve na biologia da traça-das-crucíferas; analisar a composição química das plantas, em relação aos glucosinolatos e aplicar a análise multivariada na classificação das cultivares. Foram utilizadas as cultivares: de repolho verde Chato-de-quintal e o híbrido Midori; de repolho roxo Roxo precoce e o híbrido Roxo TPC00682; e de couve-manteiga Geórgia (padrão de suscetibilidade) e Geórgia híbrido HS20. Foram avaliadas as características biológicas da praga: viabilidade e duração das fases larval e pupal, razão sexual, fecundidade das fêmeas, longevidade dos adultos e duração e viabilidade da fase de ovo, tendo-se calculado o potencial reprodutivo corrigido (PRC). Obtiveram-se as correlações entre os parâmetros pelo método de Pearson, e realizaram-se análises multivariadas de agrupamento e de componentes principais. Em cromatógrafo líquido, avaliaram-se a presença de sinigrina e de outros glucosinolatos nas plantas. A cultivar Geórgia e os híbridos HS20 e Roxo foram classificados como altamente suscetíveis; 'Roxo precoce' e 'Midori' como suscetíveis e, 'Chato-de-quintal' como moderadamente resistente à Plutella xylostella. As análises multivariadas proporcionam melhor classificação das cultivares, em razão do grau de resistência apresentado. Os materiais genéticos avaliados não apresentam a substância secundária sinigrina

A REDUCED KINETIC MECHANISM FOR PROPANE FLAMES

Propane is one of the simplest hydrocarbons that can be a representative of higher hydrocarbons used in many applications. Therefore, this work develops a ten-step reduced kinetic mechanism among 14 reactive species for the propane combustion. The model is based on the solution of the flamelet equations. The equations are discretized using the second-order space finite difference method, using LES (Large-Eddy Simulation). Obtained results compare favorably with data in the literature for a propane jet diffusion flame. The main advantage of this strategy is the decrease of the work needed to solve the system of governing equations

Desenvolvimento de metodologia de criação e multiplicação de Aphis gossypii: avanços e sucessos.

Estudos sobre as mais diversas formas de controle de pragas e utilização de inimigos naturais, exigem artrópodes em quantidade e qualidade

Capacidade predatória de Orius insidiosus predando Aphis gossypii sob o efeito da temperatura e variação da umidade relativa e fotoperíodo.

Estudou-se a capacidade predatória de Orius insidiosus predando o pulgão do algodoeiro Aphis gossypii sob o efeito da temperatura (25ºC) em diferentes umidades relativas (50, 60 e 70%) e fotoperíodos (10:14; 14:10 e 12:12 de luz:escuro). Folhas de algodoeiro cultivar DeltaOpal foram individualizada e infestada com quinze ninfas de terceiro/quarto estádio de A. gossypii e em seguida liberado um adulto do predador O.insidiosus. As avaliações foram realizadas diariamente, quantificando-se sobrevivência, longevidade e o número de pulgões predados por dia e total. A sobrevivência do predador foi afetada pelos fotoperíodos, enquanto que quando mantidos em umidade relativa de 50%, houve uma taxa menor de sobrevivência. A longevidade foi menor quando o predador estava condicionado ao fotoperíodo de 14:10 (luz:escuro). As condições testadas influenciaram na taxa diária e total de predação para fase adulta de O. insidiosus. O predador quando submetido a alta umidade relativa (70%) favoreceu maior taxa de predação

Aspectos biológicos de fêmeas adultas de Orius insidiosus (SAY, 1832) (HEMIPTERA: ANTHOCORIDAE) alimentadas com diferentes densidades de Aphis gossypii (GLOVER, 1877) (HEMIPTERA: APHIDIDAE)

Orius insidiosus (SAY, 1832) é uma espécie de predador importante para programas de controle biológico

Modelagem Numérica do Vento Atmosférico pelo Método de Decomposição Espectral

Modelagem numérica do vento atmosférico pelo métodode decomposição espectra

Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells.

Antiestrogens used for breast cancer (BC) treatment differ among each other for the ability to affect estrogen receptor (ER) activity and thereby inhibit hormone-responsive cell functions and viability. We used high-density cDNA microarrays for a comprehensive definition of the gene pathways affected by 17b-estradiol (E2), ICI 182,780 (ICI), 4OH- tamoxifen (Tamoxifen), and raloxifene (RAL) in ER-positive ZR-75.1 cells, a suitable model to investigate estrogen and antiestrogen actions in hormone-responsive BC. The expression of 601 genes was significantly affected by E2 in these cells; in silico analysis reveals that 86 among them include one or more potential ER binding site within or near the promoter and that the binding site signatures for E2F-1, NF-Y, and NRF-1 transcription factors are significantly enriched in the promoters of genes induced by estrogen treatment, while those for CAC-binding protein and LF-A1 in those repressed by the hormone, pointing to novel transcriptional effectors of secondary responses to estrogen in BC cells. Interestingly, expression of 176 E2- regulated mRNAs was unaffected by any of the antiestrogens tested, despite the fact that under the same conditions the transcriptional and cell cycle stimulatory activities of ER were inhibited. On the other hand, of 373 antiestrogen-responsive genes identified here, 52 were unresponsive to estrogen and 25% responded specifically to only one of the compounds tested, revealing non-overlapping and clearly distinguishable effects of the different antiestrogens in BC cells. As some of these differences reflect specificities of the mechanism of action of the antiestrogens tested, we propose to exploit this gene set for characterization of novel hormonal antagonists and selective estrogen receptor modulators (SERMs) and as a tool for testing new associations of antiestrogens, more effective against BC

Influence of the serotonin transporter 5HTTLPR polymorphism on symptom severity in irritable bowel syndrome

5HTTLPR polymorphism of serotonin transporter yields short (S) and long (L) alleles. SS and LS genotypes are associated with reduced expression of serotonin transporter. This cross-sectional study investigated the association of 5HTTLPR with symptom severity of irritable bowel syndrome (IBS). Patients with IBS (Rome III) and healthy controls were included. Genomic DNA was extracted from saliva, and 5HTTLPR alleles were assessed by polymerase chain reaction. IBS symptom severity was evaluated by means of IBS-SSS questionnaire. Two hundreds and four IBS patients (159 females; mean age: 39.6±12.3 years; 106 with constipation: C-IBS; 98 with diarrhea: D-IBS) and 200 healthy controls (154 females; mean age: 40.4±15.8 years) were enrolled. The overall IBS-SSS value was higher in LS/SS than LL patients (319.0±71.5 versus 283.8±62.3; P = 0.0006). LS/SS patients had also higher values of abdominal pain (59.7±21.0 versus 51.0±18.8; P = 0.020) and bowel dissatisfaction (80.1±23.9 versus 70.5±22.8; P = 0.035). The overall IBS-SSS values in C-IBS and D-IBS patients were 317.2±68.3 and 296.1±71.4, respectively (P = 0.192), with significantly higher values for abdominal distension (65.0±24.4 versus 51.4±24.8; P = 0.0006), but not for bowel dissatisfaction (80.5±21.7 versus 72.9±25.7; P = 0.138). Frequencies of 5HTTLPR genotypes did not differ significantly when comparing IBS patients (overall or upon stratification in C-IBS and D-IBS) with healthy controls. In conclusion, the LS and SS genotypes are significantly correlated with IBS symptom severity, although their possible direct causal role remains to be proven. In addition, the present findings do not support an association of 5HTTLPR with IBS or its clinical presentation in terms of bowel habit predominance