34 research outputs found
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The concurrent programming of saccades
Sequences of saccades have been shown to be prepared concurrently however it remains unclear exactly what aspects of those saccades are programmed in parallel. To examine this participants were asked to make one or two target-driven saccades: a reflexive saccade; a voluntary saccade; a reflexive then a voluntary saccade; or vice versa. During the first response the position of a second target was manipulated. The new location of the second saccade target was found to impact on second saccade latencies and second saccade accuracy showing that some aspects of the second saccade program are prepared in parallel with the first. However, differences were found in the specific pattern of effects for each sequence type. These differences fit well within a general framework for saccade control in which a common priority map for saccade control is computed and the influence of saccade programs on one another depends not so much on the types of saccade being produced but rather on the rate at which their programs develop
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The programming of sequences of saccades
Saccadic eye movements move the high-resolution fovea to point at regions of interest. Saccades can only be generated serially (i.e., one at a time). However, what remains unclear is the extent to which saccades are programmed in parallel (i.e., a series of such moments can be planned together) and how far ahead such planning occurs. In the current experiment, we investigate this issue with a saccade contingent preview paradigm. Participants were asked to execute saccadic eye movements in response to seven small circles presented on a screen. The extent to which participants were given prior information about target locations was varied on a trial-by-trial basis: participants were aware of the location of the next target only, the next three, five, or all seven targets. The addition of new targets to the display was made during the saccade to the next target in the sequence. The overall time taken to complete the sequence was decreased as more targets were available up to all seven targets. This was a result of a reduction in the number of saccades being executed and a reduction in their saccade latencies. Surprisingly, these results suggest that, when faced with a demand to saccade to a large number of target locations, saccade preparation about all target locations is carried out in paralle
Evolutionary factors affecting Lactate dehydrogenase A and B variation in the Daphnia pulex species complex
Background: Evidence for historical, demographic and selective factors affecting enzyme evolution can be
obtained by examining nucleotide sequence variation in candidate genes such as Lactate dehydrogenase (Ldh). Two
closely related Daphnia species can be distinguished by their electrophoretic Ldh genotype and habitat. Daphnia
pulex populations are fixed for the S allele and inhabit temporary ponds, while D. pulicaria populations are fixed for
the F allele and inhabit large stratified lakes. One locus is detected in most allozyme surveys, but genome
sequencing has revealed two genes, LdhA and LdhB.
Results: We sequenced both Ldh genes from 70 isolates of these two species from North America to determine if
the association between Ldh genotype and habitat shows evidence for selection, and to elucidate the evolutionary
history of the two genes. We found that alleles in the pond-dwelling D. pulex and in the lake-dwelling D. pulicaria
form distinct groups at both loci, and the substitution of Glutamine (S) for Glutamic acid (F) at amino acid 229
likely causes the electrophoretic mobility shift in the LDHA protein. Nucleotide diversity in both Ldh genes is much
lower in D. pulicaria than in D. pulex. Moreover, the lack of spatial structuring of the variation in both genes over a
wide geographic area is consistent with a recent demographic expansion of lake populations. Neutrality tests
indicate that both genes are under purifying selection, but the intensity is much stronger on LdhA.
Conclusions: Although lake-dwelling D. pulicaria hybridizes with the other lineages in the pulex species complex,
it remains distinct ecologically and genetically. This ecological divergence, coupled with the intensity of purifying
selection on LdhA and the strong association between its genotype and habitat, suggests that experimental
studies would be useful to determine if variation in molecular function provides evidence that LDHA variants are
adaptive
A first AFLP-based genetic linkage map for brine shrimp Artemia franciscana and its application in mapping the sex locus
We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp Artemia franciscana. Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2n = 42), covering 1,041 and 1,313 cM respectively. Fifteen putatively homologous linkage groups, including the sex linkage groups, were identified between the female and male linkage map. Eight sex-linked AFLP marker alleles were inherited from the female parent, supporting the hypothesis of a WZ-ZZ sex-determining system. The haploid Artemia genome size was estimated to 0.93 Gb by flow cytometry. The produced Artemia linkage maps provide the basis for further fine mapping and exploring of the sex-determining region and are a possible marker resource for mapping genomic loci underlying phenotypic differences among Artemia species
Diversity in the Reproductive Modes of European Daphnia pulicaria Deviates from the Geographical Parthenogenesis
10 páginas, 5 figuras, 3 tablas.Background: Multiple transitions to obligate parthenogenesis have occurred in the Daphnia pulex complex in North
America. These newly formed asexual lineages are differentially distributed being found predominantly at high latitudes.
This conforms to the rule of geographical parthenogenesis postulating prevalence of asexuals at high latitudes and
altitudes. While the reproductive mode of high-latitude populations is relatively well studied, little is known about the
reproduction mode in high altitudes. This study aimed to assess the reproductive mode of Daphnia pulicaria, a species of
the D. pulex complex, from high altitude lakes in Europe.
Methodology/Principal Findings: Variation at eight microsatellite loci revealed that D. pulicaria from the High Tatra
Mountains (HTM) had low genotype richness and showed excess of heterozygotes and significant deviations from Hardy-
Weinberg expectations, and was thus congruent with reproduction by obligate parthenogenesis. By contrast, populations
from the Pyrenees (Pyr) were generally in Hardy-Weinberg equilibrium and had higher genotypic richness, suggesting that
they are cyclic parthenogens. Four lakes from lowland areas (LLaP) had populations with an uncertain or mixed breeding
mode. All D. pulicaria had mtDNA ND5 haplotypes of the European D. pulicaria lineage. Pyr were distinct from LLaP and
HTM at the ND5 gene. By contrast, HTM shared two haplotypes with LLaP and one with Pyr. Principal Coordinate Analysis of
the microsatellite data revealed clear genetic differentiation into three groups. HTM isolates were intermediate to Pyr and
LLaP, congruent with a hybrid origin.
Conclusion/Significance: Inferred transitions to obligate parthenogenesis have occurred only in HTM, most likely as a result
of hybridizations. In contrast to North American populations, these transitions do not appear to involve meiosis suppressor
genes and have not been accompanied by polyploidy. The absence of obligate parthenogenesis in Pyr, an environment
highly similar to the HTM, may be due to the lack of opportunities for hybridization.Peer reviewe
Recent progress and challenges in population genetics of polyploid organisms: an overview of current state-of-the-art molecular and statistical tools
Despite the importance of polyploidy and the increasing availability of new genomic data, there remain important gaps in our knowledge of polyploid population genetics. These gaps arise from the complex nature of polyploid data (e.g. multiple alleles and loci, mixed inheritance patterns, association between ploidy and mating system variation). Furthermore, many of the standard tools for population genetics that have been developed for diploids are often not feasible for polyploids. This review aims to provide an overview of the state-of-the-art in polyploid population genetics and to identify the main areas where further development of molecular techniques and statistical theory is required. We review commonly used molecular tools (amplified fragment length polymorphism, microsatellites, Sanger sequencing, next-generation sequencing and derived technologies) and their challenges associated with their use in polyploid populations: that is, allele dosage determination, null alleles, difficulty of distinguishing orthologues from paralogues and copy number variation. In addition, we review the approaches that have been used for population genetic analysis in polyploids and their specific problems. These problems are in most cases directly associated with dosage uncertainty and the problem of inferring allele frequencies and assumptions regarding inheritance. This leads us to conclude that for advancing the field of polyploid population genetics, most priority should be given to development of new molecular approaches that allow efficient dosage determination, and to further development of analytical approaches to circumvent dosage uncertainty and to accommodate ‘flexible’ modes of inheritance. In addition, there is a need for more simulation-based studies that test what kinds of biases could result from both existing and novel approaches
Reticulate evolution of the Daphnia pulex complex as revealed by nuclear markers.
17 páginas, 3 figuras, 4 tablas.The study of species complexes is of particular interest to understand how evolutionary
young species maintain genomic integrity. The Daphnia pulex complex has been
intensively studied as it includes species that dominate freshwater environments in the
Northern hemisphere and as it is the sole North American complex that shows transitions
to obligate parthenogenesis. Past studies using mitochondrial markers have revealed the
presence of 10 distinct lineages in the complex. This study is the first to examine genetic
relationships among seven species of the complex at nuclear markers (nine microsatellite
loci and one protein-coding gene). Clones belonging to the seven species of the Daphnia
pulex complex were characterized at the mitochondrial NADH dehydrogenase (ND5)
gene and at the Lactate dehydrogenase (LDH) locus. K-means, principal coordinate
analyses and phylogenetic network analyses on the microsatellite data all separated
European D. pulicaria, D. tenebrosa, North American D. pulex, D. pulicaria and their
hybrids into distinct clusters. The hybrid cluster was composed of diploid and polyploid
hybrids with D. pulex mitochondria and some clones with D. pulicaria mitochondria. By
contrast, the phylogeny of the D. pulex complex using Rab4 was not well resolved but
still showed clusters consisting mostly of D. pulex alleles and others of D. pulicaria
alleles. Incomplete lineage sorting and hybridization may obscure genetic relationships
at this locus. This study shows that hybridization and introgression have played an
important role in the evolution of this complex.This work was supported by a Natural Sciences and Engineering research Council (NSERC) research grants and by a Canadian Foundation for Innovation (CFI) equipment grants to
France Dufresne. Additional support to Silvia Markova was
provided by the Academy of Sciences of the Czech Republic
(grants IRP IAPG AV0Z 50450515 and KJB600450901)Peer reviewe
Eye movements when reading disappearing text: Is there a gap effect in reading?
Readers’ eye movements were monitored when they read either normal sentences or sentences with masked or disappearing text (in which the fixated word disappeared or was masked after 60 ms). The goals of the research were to investigate (1) whether a gap effect occurred in reading and (2) the influence of linguistic and visual factors on oculomotor control. The results of a number of global analyses of eye movements under disappearing text conditions clearly demonstrated that there is no gap effect in reading. However, comparative analyses across a number of local measures in the experiments indicated that cognitive/lexical processes, as well as the continual uptake of visual information, influence eye movement control during reading. A persistent visual object throughout fixation caused refixations and even when a fixated word had disappeared (or been masked), there were significant effects of word frequency and word length
Cold adaptation in geographical populations of <i>Drosophila melanogaster</i> phenotypic plasticity is more important than genetic variability
1. According to their geographical distribution, most Drosophila species may be classified as either temperate or tropical, and this pattern is assumed to reflect differences in their thermal adaptation, especially in their cold tolerance. We investigated cold tolerance in a global collection of D. melanogaster by monitoring the time adults take to recover from chill coma after a treatment at 0degreesC. 2. Flies grown at an intermediate temperature (21degreesC) showed a significant linear latitudinal cline: recovery was faster in populations living in colder climates. 3. The role of growth temperature was analysed in a subset of tropical and temperate populations. In all cases, recovery time decreased when growth temperature was lowered, and linear reaction norms were observed. This adaptive phenotypic plasticity explained more than 80% of the total variation, while genetic latitudinal differences accounted for less than 4%. 4. The beneficial effect observed in adults grown at a low temperature contrasts with other phenotypic effects which, like male sterility, appear as harmful and pathological. Our results point to the difficulty of finding a general interpretation to the diversity of plastic responses that are induced by growth temperature variations
Cold adaptation in geographical populations of Drosophila melanogaster:Phenotypic plasticity is more important than genetic variability
1. According to their geographical distribution, most Drosophila species may be classified as either temperate or tropical, and this pattern is assumed to reflect differences in their thermal adaptation, especially in their cold tolerance. We investigated cold tolerance in a global collection of D. melanogaster by monitoring the time adults take to recover from chill coma after a treatment at 0degreesC. 2. Flies grown at an intermediate temperature (21degreesC) showed a significant linear latitudinal cline: recovery was faster in populations living in colder climates. 3. The role of growth temperature was analysed in a subset of tropical and temperate populations. In all cases, recovery time decreased when growth temperature was lowered, and linear reaction norms were observed. This adaptive phenotypic plasticity explained more than 80% of the total variation, while genetic latitudinal differences accounted for less than 4%. 4. The beneficial effect observed in adults grown at a low temperature contrasts with other phenotypic effects which, like male sterility, appear as harmful and pathological. Our results point to the difficulty of finding a general interpretation to the diversity of plastic responses that are induced by growth temperature variations