436 research outputs found

    What languages are chosen in Malaysian family domain? The case of Malay, Chinese and Indian undergraduates.

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    People in multilingual societies choose and use language depending on the contexts, activities, role relationships, gender and other ethno-linguistic background. Malaysia being a multilingual and multicultural country, what languages are chosen in the family domain constitutes the focus and objectives of this paper. The patterns of language choice among the university undergraduates of Malay, Chinese and Indian ethnic groups in the family domain were obtained through a questionnaire survey administered in November and December, 2006 at the University Putra Malaysia and the data were analyzed using SPSS. It is found that ethnic languages were found to be preferred in the family domain. Bahasa, Melayu and English were also reported to be chosen in this domain which could be attributed to domain allocation of language use and role-relationships among the family members. Ethnicity and proficiency were also found to play important role in the choice of language

    Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags

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    <p>Abstract</p> <p>Background</p> <p>The Red-headed krait (<it>Bungarus flaviceps</it>, Squamata: Serpentes: Elapidae) is a medically important venomous snake that inhabits South-East Asia. Although the venoms of most species of the snake genus <it>Bungarus </it>have been well characterized, a detailed compositional analysis of <it>B. flaviceps </it>is currently lacking.</p> <p>Results</p> <p>Here, we have sequenced 845 expressed sequence tags (ESTs) from the venom gland of a <it>B. flaviceps</it>. Of the transcripts, 74.8% were putative toxins; 20.6% were cellular; and 4.6% were unknown. The main venom protein families identified were three-finger toxins (3FTxs), Kunitz-type serine protease inhibitors (including chain B of β-bungarotoxin), phospholipase A<sub>2 </sub>(including chain A of β-bungarotoxin), natriuretic peptide (NP), CRISPs, and C-type lectin.</p> <p>Conclusion</p> <p>The 3FTxs were found to be the major component of the venom (39%). We found eight groups of unique 3FTxs and most of them were different from the well-characterized 3FTxs. We found three groups of Kunitz-type serine protease inhibitors (SPIs); one group was comparable to the classical SPIs and the other two groups to chain B of β-bungarotoxins (with or without the extra cysteine) based on sequence identity. The latter group may be functional equivalents of dendrotoxins in <it>Bungarus </it>venoms. The natriuretic peptide (NP) found is the first NP for any Asian elapid, and distantly related to Australian elapid NPs. Our study identifies several unique toxins in <it>B. flaviceps </it>venom, which may help in understanding the evolution of venom toxins and the pathophysiological symptoms induced after envenomation.</p

    Comparative population genetics of mimetic Heliconius butterflies in an endangered habitat; Brazil's Atlantic Forest

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    <p>Abstract</p> <p>Background</p> <p>Brazil's Atlantic Forest is a biodiversity hotspot endangered by severe habitat degradation and fragmentation. Habitat fragmentation is expected to reduce dispersal among habitat patches resulting in increased genetic differentiation among populations. Here we examined genetic diversity and differentiation among populations of two <it>Heliconius </it>butterfly species in the northern portion of Brazil's Atlantic Forest to estimate the potential impact of habitat fragmentation on population connectivity in butterflies with home-range behavior.</p> <p>Results</p> <p>We generated microsatellite, AFLP and mtDNA sequence data for 136 <it>Heliconius erato </it>specimens from eight collecting locations and 146 <it>H. melpomene </it>specimens from seven locations. Population genetic analyses of the data revealed high levels of genetic diversity in <it>H. erato </it>relative to <it>H. melpomene</it>, widespread genetic differentiation among populations of both species, and no evidence for isolation-by-distance.</p> <p>Conclusions</p> <p>These results are consistent with the hypothesis that the extensive habitat fragmentation along Brazil's Atlantic Forest has reduced dispersal of <it>Heliconius </it>butterflies among neighboring habitat patches. The results also lend support to the observation that fine-scale population genetic structure may be common in <it>Heliconius</it>. If such population structure also exists independent of human activity, and has been common over the evolutionary history of <it>Heliconius </it>butterflies, it may have contributed to the evolution of wing pattern diversity in the genus.</p

    Design and Implementation of Lab Scale Automated Solar Powered Irrigation and Fertigation System

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    In Brunei, natural oil and gas contributes to about 99% in the generation of electricity and about 88% to the country revenue. Since Brunei is heavily dependent on a non-renewable energy, potential exhaustion of oil and gas reserves pose a challenge to the country, especially with continuous increase of energy consumption in order to meet the population demand. Additionally, Brunei has ambition to improve its agriculture sector. Implementations of PV systems in local agricultural sectors can also help improving the country revenue by increasing yield and reducing costs from electricity consumption. In this paper, we present design and practical implementation of fully automated solar powered irrigation and fertigation system. Ultrasonic sensors and moisture sensors are used to detect the state of the tank and the soil. The whole system is controlled with a microcontroller and a wireless monitoring system with mobile application is designed. The operation of the system was tested and the performance was acceptable

    Survey of rehabilitation support for children 0-15 years in a rural part of Kenya

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    Abstract Purpose: Information regarding the nature, availability and distribution of rehabilitation services for children with disabilities across developing countries is scarce, and data that do exist are of variable quality. If planning and development are to progress, information about service provision is vital. The aim was to establish the scope and nature of rehabilitation support available to children with disabilities (0-15 years) and their families in rural Kenya. Method: A comprehensive sample comprising service provision in the health and special education sectors was established. Non-governmental and community-based organisations were also included. A survey of rehabilitation services was conducted through examination of service-related documentation and key informant interviews with the heads of services. Results: Rehabilitation comprised hospital-based occupational therapy, physiotherapy and orthopaedic technology; and seven special education establishments plus an education assessment resource centre. There was one non-government organisation and one community-based organisation relevant to children with disabilities. Activities focused on assessment, diagnosis and raising community awareness. Provision was challenged by inadequate staffing, resources and transport. Government funding was supplemented variously by donations and self-sufficiency initiatives. Rehabilitation approaches appeared to be informed by professional background of practitioner, rather than the needs of child. Service documentation revealed use of inconsistent recording methods. Conclusions: The data highlight the challenges of rehabilitation, demanding greater investment in personnel and their training, more material resources, improved access to the community and better recording mechanisms. Implications for Rehabilitation There needs to be greater investment in rehabilitation provision in developing countries. Consideration of community-based initiatives is required to support better access for all. In order to argue the case for improved resources, better skills and mechanisms for recording, monitoring and evaluating practice are needed

    Identification of harmful cyanobacteria in the Sacramento-San Joaquin Delta and Clear Lake, California by DNA barcoding.

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    Accurate identification of cyanobacteria using traditional morphological taxonomy is challenging due to the magnitude of phenotypic plasticity among natural algal assemblages. In this study, molecular approach was utilized to facilitate the accurate identification of cyanobacteria in the Sacramento-San Joaquin Delta and in Clear Lake in Northern California where recurring blooms have been observed over the past decades. Algal samples were collected from both water bodies in 2011 and the samples containing diverse cyanobacteria as identified by morphological taxonomy were chosen for the molecular analysis. The 16S ribosomal RNA genes (16S rDNA) and the adjacent internal transcribed spacer (ITS) regions were amplified by PCR from the mixed algal samples using cyanobacteria generic primers. The obtained sequences were analyzed by similarity search (BLASTN) and phylogenetic analysis (16S rDNA) to differentiate species sharing significantly similar sequences. A total of 185 plasmid clones were obtained of which 77 were successfully identified to the species level: Aphanizomenon flos-aquae, Dolichospermum lemmermannii (taxonomic synonym: Anabaena lemmermannii), Limnoraphis robusta (taxonomic synonym: Lyngbya hieronymusii f. robusta) and Microcystis aeruginosa. To date, Dolichospermum and Limnoraphis found in Clear Lake have only been identified to the genus lavel by microscopy. During the course of this study, morphological identification and DNA barcoding confirmed A. flos-aquae as the predominant cyanobacterium in the Sacramento-San Joaquin Delta indicating a shift from M. aeruginosa that have dominated the blooms in the past decade. Lastly, the species-specific identification of Limnoraphis robusta in Clear Lake is another significant finding as this cyanobacterium has, thus far, only been reported in Lake Atitlan blooms in Guatemala
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