580 research outputs found
Multi-Omics and Genome Editing Studies on Plant Cell Walls to Improve Biomass Quality
Biomass is one of the most important sources of renewable energy and plays an important role in reducing our reliance on fossil fuels. Efficient biomass production is essential to obtain large amounts of sustainable energy with minimal environmental cost. However, the biochemical and molecular processes behind the synthesis of the main components of biomass are still not fully understood. This review provides a comprehensive summary of the most relevant studies on cell wall biosynthesis and degradation mechanisms, focusing on the lignocellulosic component, in which the conversion process to fermentable sugars is expensive, due to its recalcitrant nature. A focus is placed on multi-omics research involving genomics, transcriptomics, proteomics, metabolomics, and phenomics, since multi-omics approaches offer a unique opportunity to investigate the biological pathways underlying the genotype traits characterizing cell wall energy crops. Furthermore, our study highlights the advances in genome editing approaches and proposes the modification of the genes that are involved in the complex cell wall structure as a feasible solution to an efficient biomass production. Several key points for future research activities based on these emerging technologies are also discussed, focusing on the combination of multi-omics and gene editing approaches, which offer potential for improved biomass valorization and the development of tangible bioproducts
Adiabaticity Conditions for Volatility Smile in Black-Scholes Pricing Model
Our derivation of the distribution function for future returns is based on
the risk neutral approach which gives a functional dependence for the European
call (put) option price, C(K), given the strike price, K, and the distribution
function of the returns. We derive this distribution function using for C(K) a
Black-Scholes (BS) expression with volatility in the form of a volatility
smile. We show that this approach based on a volatility smile leads to relative
minima for the distribution function ("bad" probabilities) never observed in
real data and, in the worst cases, negative probabilities. We show that these
undesirable effects can be eliminated by requiring "adiabatic" conditions on
the volatility smile
A Calibration of NICMOS Camera 2 for Low Count-Rates
NICMOS 2 observations are crucial for constraining distances to most of the
existing sample of z > 1 SNe Ia. Unlike the conventional calibration programs,
these observations involve long exposure times and low count rates. Reciprocity
failure is known to exist in HgCdTe devices and a correction for this effect
has already been implemented for high and medium count-rates. However
observations at faint count-rates rely on extrapolations. Here instead, we
provide a new zeropoint calibration directly applicable to faint sources. This
is obtained via inter-calibration of NIC2 F110W/F160W with WFC3 in the low
count-rate regime using z ~ 1 elliptical galaxies as tertiary calibrators.
These objects have relatively simple near-IR SEDs, uniform colors, and their
extended nature gives superior signal-to-noise at the same count rate than
would stars. The use of extended objects also allows greater tolerances on PSF
profiles. We find ST magnitude zeropoints (after the installation of the NICMOS
cooling system, NCS) of 25.296 +- 0.022 for F110W and 25.803 +- 0.023 for
F160W, both in agreement with the calibration extrapolated from count-rates
1,000 times larger (25.262 and 25.799). Before the installation of the NCS, we
find 24.843 +- 0.025 for F110W and 25.498 +- 0.021 for F160W, also in agreement
with the high-count-rate calibration (24.815 and 25.470). We also check the
standard bandpasses of WFC3 and NICMOS 2 using a range of stars and galaxies at
different colors and find mild tension for WFC3, limiting the accuracy of the
zeropoints. To avoid human bias, our cross-calibration was "blinded" in that
the fitted zeropoint differences were hidden until the analysis was finalized.Comment: Accepted for Publication in the Astronomical Journal. New version
contains added referenc
A New Determination of the High Redshift Type Ia Supernova Rates with the Hubble Space Telescope Advanced Camera for Surveys
We present a new measurement of the volumetric rate of Type Ia supernova up
to a redshift of 1.7, using the Hubble Space Telescope (HST) GOODS data
combined with an additional HST dataset covering the North GOODS field
collected in 2004. We employ a novel technique that does not require
spectroscopic data for identifying Type Ia supernovae (although spectroscopic
measurements of redshifts are used for over half the sample); instead we employ
a Bayesian approach using only photometric data to calculate the probability
that an object is a Type Ia supernova. This Bayesian technique can easily be
modified to incorporate improved priors on supernova properties, and it is
well-suited for future high-statistics supernovae searches in which
spectroscopic follow up of all candidates will be impractical. Here, the method
is validated on both ground- and space-based supernova data having some
spectroscopic follow up. We combine our volumetric rate measurements with low
redshift supernova data, and fit to a number of possible models for the
evolution of the Type Ia supernova rate as a function of redshift. The data do
not distinguish between a flat rate at redshift > 0.5 and a previously proposed
model, in which the Type Ia rate peaks at redshift >1 due to a significant
delay from star-formation to the supernova explosion. Except for the highest
redshifts, where the signal to noise ratio is generally too low to apply this
technique, this approach yields smaller or comparable uncertainties than
previous work.Comment: Accepted for publication in Ap
Genetic microheterogeneity and phenotypic variation of Helicobacter pylori arginase in clinical isolates
BACKGROUND: Clinical isolates of the gastric pathogen Helicobacter pylori display a high level of genetic macro- and microheterogeneity, featuring a panmictic, rather than clonal structure. The ability of H. pylori to survive the stomach acid is due, in part, to the arginase-urease enzyme system. Arginase (RocF) hydrolyzes L-arginine to L-ornithine and urea, and urease hydrolyzes urea to carbon dioxide and ammonium, which can neutralize acid. RESULTS: The degree of variation in arginase was explored at the DNA sequence, enzyme activity and protein expression levels. To this end, arginase activity was measured from 73 minimally-passaged clinical isolates and six laboratory-adapted strains of H. pylori. The rocF gene from 21 of the strains was cloned into genetically stable E. coli and the enzyme activities measured. Arginase activity was found to substantially vary (>100-fold) in both different H. pylori strains and in the E. coli model. Western blot analysis revealed a positive correlation between activity and amount of protein expressed in most H. pylori strains. Several H. pylori strains featured altered arginase activity upon in vitro passage. Pairwise alignments of the 21 rocF genes plus strain J99 revealed extensive microheterogeneity in the promoter region and 3' end of the rocF coding region. Amino acid S232, which was I232 in the arginase-negative clinical strain A2, was critical for arginase activity. CONCLUSION: These studies demonstrated that H. pylori arginase exhibits extensive genotypic and phenotypic variation which may be used to understand mechanisms of microheterogeneity in H. pylori
Spectra of High-Redshift Type Ia Supernovae and a Comparison with their Low-Redshift Counterparts
We present spectra for 14 high-redshift (0.17 < z < 0.83) supernovae, which
were discovered by the Supernova Cosmology Project as part of a campaign to
measure cosmological parameters. The spectra are used to determine the redshift
and classify the supernova type, essential information if the supernovae are to
be used for cosmological studies. Redshifts were derived either from the
spectrum of the host galaxy or from the spectrum of the supernova itself. We
present evidence that these supernovae are of Type Ia by matching to spectra of
nearby supernovae. We find that the dates of the spectra relative to maximum
light determined from this fitting process are consistent with the dates
determined from the photometric light curves, and moreover the spectral
time-sequence for SNe Type Ia at low and high redshift is indistinguishable. We
also show that the expansion velocities measured from blueshifted CaHK are
consistent with those measured for low-redshift Type Ia supernovae. From these
first-level quantitative comparisons we find no evidence for evolution in SNIa
properties between these low- and high-redshift samples. Thus even though our
samples may not be complete, we conclude that there is a population of SNe Ia
at high redshift whose spectral properties match those at low redshift.Comment: Accepted for publication in AJ. Also available at
http://supernova.lbl.gov
Subaru FOCAS Spectroscopic Observations of High-Redshift Supernovae
We present spectra of high-redshift supernovae (SNe) that were taken with the
Subaru low resolution optical spectrograph, FOCAS. These SNe were found in SN
surveys with Suprime-Cam on Subaru, the CFH12k camera on the
Canada-France-Hawaii Telescope (CFHT), and the Advanced Camera for Surveys
(ACS) on the Hubble Space Telescope (HST). These SN surveys specifically
targeted z>1 Type Ia supernovae (SNe Ia). From the spectra of 39 candidates, we
obtain redshifts for 32 candidates and spectroscopically identify 7 active
candidates as probable SNe Ia, including one at z=1.35, which is the most
distant SN Ia to be spectroscopically confirmed with a ground-based telescope.
An additional 4 candidates are identified as likely SNe Ia from the
spectrophotometric properties of their host galaxies. Seven candidates are not
SNe Ia, either being SNe of another type or active galactic nuclei. When SNe Ia
are observed within a week of maximum light, we find that we can
spectroscopically identify most of them up to z=1.1. Beyond this redshift, very
few candidates were spectroscopically identified as SNe Ia. The current
generation of super red-sensitive, fringe-free CCDs will push this redshift
limit higher.Comment: 19 pages, 26 figures. PASJ in press. see
http://www.supernova.lbl.gov/2009ClusterSurvey/ for additional information
pertaining to the HST Cluster SN Surve
Laser treatment monitoring with reflectance confocal microscopy
Laser treatments have become popular in Dermatology. In parallel to technologic development enabling the availability of different laser wavelengths, non-invasive skin imaging techniques, such as reflectance confocal microscopy (RCM), have been used to explore morphologic and qualitative skin characteristics. Specifically, RCM can be applied to cosmetically sensitive skin areas such as the face, without the need for skin biopsies. For these reasons, apart from its current use in skin cancer diagnosis, our systematic review reveals how RCM can be employed in the field of laser treatment monitoring, being particularly suitable for the evaluation of variations in epidermis and dermis, and pigmentary and vascular characteristics of the skin. This systematic review article aims to provide an overview on current applications of RCM laser treatment monitoring, while describing RCM features identified for different applications. Studies on human subjects treated with laser treatments, monitored with RCM, were included in the current systematic review. Five groups of treatments were identified and described: skin rejuvenation, scar tissue, pigmentary disorders, vascular disorders and other. Interestingly, RCM can assist treatments with lasers targeting all chromophores in the skin and exploiting laser induced optical breakdown. Treatment monitoring encompasses assessment at baseline and examination of changes after treatment, therefore revealing details in morphologic alterations underlying different skin conditions and mechanisms of actions of laser therapy, as well as objectify results after treatment
New Results from the Cryogenic Dark Matter Search Experiment
Using improved Ge and Si detectors, better neutron shielding, and increased
counting time, the Cryogenic Dark Matter Search (CDMS) experiment has obtained
stricter limits on the cross section of weakly interacting massive particles
(WIMPs) elastically scattering from nuclei. Increased discrimination against
electromagnetic backgrounds and reduction of neutron flux confirm
WIMP-candidate events previously detected by CDMS were consistent with neutrons
and give limits on spin-independent WIMP interactions which are >2X lower than
previous CDMS results for high WIMP mass, and which exclude new parameter space
for WIMPs with mass between 8-20 GeV/c^2.Comment: 4 pages, 4 figure
Spectroscopic Observations and Analysis of the Unusual Type Ia SN 1999ac
We present optical spectra of the peculiar Type Ia supernova (SN Ia) 1999ac.
The data extend from -15 to +42 days with respect to B-band maximum and reveal
an event that is unusual in several respects. Prior to B-band maximum, the
spectra resemble those of SN 1999aa, a slowly declining event, but possess
stronger SiII and CaII signatures (more characteristic of a spectroscopically
normal SN). Spectra after B-band maximum appear more normal. The expansion
velocities inferred from the Iron lines appear to be lower than average;
whereas, the expansion velocity inferred from Calcium H and K are higher than
average. The expansion velocities inferred from SiII are among the slowest ever
observed, though SN 1999ac is not particularly dim. The analysis of the
parameters v_10, R(SiII), dv(SiII)/dt, and d_m15 further underlines the unique
characteristics of SN 1999ac. We find convincing evidence of CII 6580 in the
day -15 spectrum with ejection velocity v > 16,000 km/s, but this signature
disappears by day -9. This rapid evolution at early times highlights the
importance of extremely early-time spectroscopy.Comment: 40 pages, 24 figures, accepted for publication in The Astronomical
Journa
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