109 research outputs found
Design of automated system for management of seminars at national institute of technology,rourkela
National Institute of Technology, Rourkela is one of the reputed institutions for technical education in India covering an area of 262 hectares. The main objective of the project is to develop a portal for management of seminars which will he hosted on our institution server. The portal provides a suitable and easy display for which the admin, department or student can view and upload the seminar details. Basically at what time, which department, which speaker, contact details etc. are provided on this portal. Using ASP.NET technologies and SQL Server, this portal is designed
Characteristics of Iron Ore Slime from Kiriburu, India
The mineralogical, size and chemical characteristics of slime vary from discharge point to the distal part of the Tailing pond of Kiriburu iron ore mines. They can be grouped as three end members of slime composition- (i) coarse grained dense martite microplaty hematite type, (ii) coarse to medium grained goethite and clay dominated martite hematite type, (iii) fine grained hematite-goethite-clay type, occurring systematically away from the discharge point. Any slime will have a bulk composition intermediate to these three end members. The study has significance in the planning for exploitation of slime
A 250 plastome phylogeny of the grass family (Poaceae): topological support under different data partitions
The systematics of grasses has advanced through applications of plastome phylogenomics, although studies have been largely limited to subfamilies or other subgroups of Poaceae. Here we present a plastome phylogenomic analysis of 250 complete plastomes (179 genera) sampled from 44 of the 52 tribes of Poaceae. Plastome sequences were determined from high throughput sequencing libraries and the assemblies represent over 28.7 Mbases of sequence data. Phylogenetic signal was characterized in 14 partitions, including (1) complete plastomes; (2) protein coding regions; (3) noncoding regions; and (4) three loci commonly used in single and multi-gene studies of grasses. Each of the four main partitions was further refined, alternatively including or excluding positively selected codons and also the gaps introduced by the alignment. All 76 protein coding plastome loci were found to be predominantly under purifying selection, but specific codons were found to be under positive selection in 65 loci. The loci that have been widely used in multi-gene phylogenetic studies had among the highest proportions of positively selected codons, suggesting caution in the interpretation of these earlier results. Plastome phylogenomic analyses confirmed the backbone topology for Poaceae with maximum bootstrap support (BP). Among the 14 analyses, 82 clades out of 309 resolved were maximally supported in all trees. Analyses of newly sequenced plastomes were in agreement with current classifications. Five of seven partitions in which alignment gaps were removed retrieved Panicoideae as sister to the remaining PACMAD subfamilies. Alternative topologies were recovered in trees from partitions that included alignment gaps. This suggests that ambiguities in aligning these uncertain regions might introduce a false signal. Resolution of these and other critical branch points in the phylogeny of Poaceae will help to better understand the selective forces that drove the radiation of the BOP and PACMAD clades comprising more than 99.9% of grass diversity
A next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics, applied to Poa grasses
<p>Abstract</p> <p>Background</p> <p>Polyploidy is important from a phylogenetic perspective because of its immense past impact on evolution and its potential future impact on diversification, survival and adaptation, especially in plants. Molecular population genetics studies of polyploid organisms have been difficult because of problems in sequencing multiple-copy nuclear genes using Sanger sequencing. This paper describes a method for sequencing a barcoded mixture of targeted gene regions using next-generation sequencing methods to overcome these problems.</p> <p>Results</p> <p>Using 64 3-bp barcodes, we successfully sequenced three chloroplast and two nuclear gene regions (each of which contained two gene copies with up to two alleles per individual) in a total of 60 individuals across 11 species of Australian <it>Poa </it>grasses. This method had high replicability, a low sequencing error rate (after appropriate quality control) and a low rate of missing data. Eighty-eight percent of the 320 gene/individual combinations produced sequence reads, and >80% of individuals produced sufficient reads to detect all four possible nuclear alleles of the homeologous nuclear loci with 95% probability.</p> <p>We applied this method to a group of sympatric Australian alpine <it>Poa </it>species, which we discovered to share an allopolyploid ancestor with a group of American <it>Poa </it>species. All markers revealed extensive allele sharing among the Australian species and so we recommend that the current taxonomy be re-examined. We also detected hypermutation in the <it>trn</it>H-<it>psb</it>A marker, suggesting it should not be used as a land plant barcode region. Some markers indicated differentiation between Tasmanian and mainland samples. Significant positive spatial genetic structure was detected at <100 km with chloroplast but not nuclear markers, which may be a result of restricted seed flow and long-distance pollen flow in this wind-pollinated group.</p> <p>Conclusions</p> <p>Our results demonstrate that 454 sequencing of barcoded amplicon mixtures can be used to reliably sample all alleles of homeologous loci in polyploid species and successfully investigate phylogenetic relationships among species, as well as to investigate phylogeographic hypotheses. This next-generation sequencing method is more affordable than and at least as reliable as bacterial cloning. It could be applied to any experiment involving sequencing of amplicon mixtures.</p
Figure S3: Maximum clade credibility tree obtained from BEAST analysis of plastid DNA sequences
The utility of Bambusoideae (Poaceae, Poales) leaf blade anatomy for identification and systematics
Establishing a baseline of plant diversity and endemism on a neotropical mountain summit for future comparative studies assessing upward migration: an approach from biogeography and nature conservation
An infrageneric classification for Poa in North America, and other notes on sections, species, and subspecies of Poa, Puccinellia, and Dissanthelium (Poaceae)
Volume: 8Start Page: 187End Page: 20
Taxonomic notes on Schedonorus, a segregate genus from Festuca or Lolium, with a new nothogenus, X Schedololium, and new combination
Volume: 83Start Page: 85End Page: 8
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