242 research outputs found

    Effects of Ascorbic Acid on Reproductive Functions of Male Wistar Rats Exposed to Nicotine

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    Nicotine is a pharmacologically active and addictive alkaloid component of the cigarette smoke, and its effects on male reproductive system and fertility are well documented. Influence of ascorbic acid on reproductive functions of male wistar rats exposed to nicotine was examined in this study. Thirty-two adult male rats of 180 ± 15 g weight were used and grouped into control, nicotine, ascorbic and nicotine with ascorbic acid. The drugs were orally administered for thirty-five days. Plasma levels of FSH, LH and testosterone were significantly reduced in nicotine exposed rats when compared with the control (p<0.05), both FSH and LH plasma levels were significantly increased in rats exposed to ascorbic acid (p<0.05) relative to the control, while ascorbic acid also increased the level of these hormone in nicotine treated group (p<0.05). The cytoarchitecture of the seminiferous tubule shows high level of degeneration in the nicotine only treated group and this was reversed in the ascorbic acid treated group. There was a significant decreased in sperm motility, counts, percentage viability and morphology (p<0.05) in nicotine treated group relative to the control, while there was a significant improvement in these sperm parameters in co-administered ascorbic acid with nicotine when compared with the rats treated with nicotine only. In conclusion, ascorbic acid supplement may suppress nicotine toxic effects on reproductive functions in male rats.Keywords: Nicotine, ascorbic acid, FSH, LH, Testosterone, Sperm parameter

    Adoption of e-voting systems: A case study of Independent National Electoral Commission (INEC), Nigeria: A preliminary study

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    The trend in the technological development has made the use of computer and its supporting technologies mandatory in virtually all aspects of life.This is never an exception in the conventional voting process.Several issues have been revealed associated with the manual voting system which makes it inappropriate in the emerging technology-driven society. Several countries have successfully implemented an electronic voting technology. Considering Nigeria as a developing nation with quite a number of constraints regarding technological innovations, there is therefore the need to investigate the readiness on the part of the organization empowered to conduct elections in the country (INEC) so as to pave ways for its successful implementation.In this paper, the relationship among the variables of adoption evidenced from the previous literature was investigated using correlation coefficients and multiple linear regressions.As a pilot study, only 47 responses were captured from senior staffs of INEC. The results show that there is strong relationship between the variables examined

    RISK SURVEILLANCE OF MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA IN WATER AND PLASMID RELATEDNESS WITH CLINICAL STRAINS IN ABEOKUTA, SOUTHWEST NIGERIA

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    Pseudomonas aeruginosa as an opportunistic pathogen has been a subject of investigation due its intrinsic drug resistance.ª¤?ª¤? Its frequent presence in drinking, domestic and recreational water highlights its significance to public health.ª¤?ª¤? This study was aimed at risk surveillance of multidrug resistant environmental P. aeruginosa in water and their plasmid relatedness with clinical strains in Abeokuta, southwestern Nigeria.ª¤?ª¤? A total of forty-one (41) strains with prevalence: well water (29.3%); swimming pool (22.0%) hospital storage tank (19.5%); tap water (14.6%); sachet water (12.2%); and bottled water (2.4%) respectively were isolated from two hundred and eighty eight (288) water samples and were compared with 43 clinical strains from wound (37.3%), blood (11.6%), ear swab (20.9%)ª¤? and urine (20.9%)ª¤? and eye swab (9.3%).ª¤?ª¤?ª¤?ª¤? Both environmental and clinical strains were all multidrug resistant, though with different plasmid profile.ª¤? Plasmid with molecular weight size of 2010bp was detected in only 1 (2.5%) out of the 41 environmental strains as against 9 (20.93%) of the 43 clinical strains having between 22520-23130bp molecular weight.ª¤? All strains harboring plasmid were resistant to varied types of more than seven drugs out of the eleven tested (gentamycin 10ª¤?g, erythromycin 15ª¤?g, ampicillin 10ª¤?g, augmentin 10ª¤?g, cotrimoxazole 25ª¤?g, tetracycline 30ª¤?g, streptomycin 10ª¤?g, ciprofloxacin 5ª¤?g, cloxacillin 5ª¤?g, amoxicillin 25ª¤?g, and cefuroxime 30ª¤?g). ª¤?Strains without plasmid were also multidrug resistant.ª¤? This finding would be important in the control of multidrug resistant Pseudomonas aeruginosa infection in Nigeria

    RANDOM AMPLIFIED POLYMORHIC DNA TYPING OF MULTIDRUG-RESISTANT CLINICAL AND ENVIRONMENTAL Pseudomonas aeruginosa STRAINS FROM ABEOKUTA, NIGERIA.

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    Pseudomonas aeruginosa, a multidrug-resistant organism is responsible for most opportunistic infections. Genetic relatedness between clinical and environmental strains has always been limited to hospital settings.ª¤? This study utilized RAPD-PCR typing method to evaluate genetic relatedness between multidrug-resistantª¤? P. aeruginosa strains from diverse water samples (bottled water, tap water, sachet water, well water, hospital storage tank, and swimming pool water) and clinical strains (wound, blood, urine, eye and ear swab) collected from different locations in Abeokuta, Nigeria.ª¤?ª¤?ª¤? Polymorphic DNA bands with sizes ranging between 250 and 3000bp were generated from both clinical and environmental strains.ª¤? Within each population, both clinical and environmental strains were divided phylogenetically into two groups of Pc1 and Pc2 at 55% and Ps1 and Ps2 at 70% respectively.ª¤?ª¤?ª¤? Genetic similarities between clinical and environmental strains yielded a total of 7 unique fingerprints.ª¤?ª¤? Cluster 2 (51.2%) had the largest number of strains in which strains from wound, blood and ear, clustered with strains from hospital storage tank, tap water, swimming pool water, sachet water and well water.ª¤? ª¤?ª¤?These fingerprints proof genetic relatedness between clinical and environmental strains in Abeokuta, southwest Nigeria which is of public health significance, particularly, for immunocompromisedª¤?individuals.ª¤?ª¤

    Effect of Methanol extract of Musca domestica larva on some Enzymes and Haematological parameters in Trypanosoma brucei brucei - infected rats

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    This study investigated the effect of methanol extract of Musca domestica (400mg/kg body weight) on some biomarker enzymes and haematological parameters in Trypanosoma brucei  brucei - infected rats. Twenty albino rats were intraperitoneally infected with Trypanosoma brucei brucei and were grouped into five (5) groups of four (4) rats each. Group1 was set up as infected not treated (0.2ml normal saline/kg body weight), group 2 was treated with diaminazene aceturate (standard drug), group 3 as prophylactic treated (treatment for 72 hours before inoculation of parasite), group 4 as early treatment with the extract (treatment commenced after the sight of parasite) and group 5 as the control (uninfected untreated) group. Results shows significant (p<0.05) decrease in liver AST and ALT activities with concomitant increase in serum activities of the infected untreated rats when compared with the early treated, prophylactic treated, standard treated and normal control. Serum ALP activity of the infected not treated group was significantly (p<0.05) higher when compared to the control group and other experimental groups. No significant (p>0.05) difference in the liver ALP activities of the extract treated infected groups with standard drug treated group However, serum and liver GGT activities of the uninfected untreated (control) was significantly lower (p<0.05) than all the other experimental groups. Haematological studies shows significant decrease (p<0.05) in packed cell volume (PCV) , haemoglobin concentration (Hb) and red blood cell count (RBC) of infected not treated when compared to infected prophylactic treated and infected early treated. There was likewise significant increase in white blood cell count (WBC) of infected not treated compared to infected prophylactic treated and infected early treated. Findings from this study showed that methanol extract of Musca domestica larva has trypanocidal properties thereby ameliorating the T. brucei induced biochemical changes in rats.Key words: Musca domestica larva, Haematology, Trypanosomiasis, Enzymes, Methanol, Extract

    Development of Fingerprint Biometric Attendance System for Non-Academic Staff in a Tertiary Institution

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    Institutions, companies and organisations where security and net productivity is vital, access to certain areas must be controlled and monitored through an automated system of attendance. Managing people is a difficult task for most of the organizations and maintaining the attendance record is an important factor in people management. When considering the academic institute, taking the attendance of non-academic staff on daily basis and maintaining the records is a major task. Manually taking attendance and maintaining it for a long time adds to the difficulty of this task as well as wastes a lot of time. For this reason, an efficient system is proposed in this paper to solve the problem of manual attendance. This system takes attendance electronically with the help of a fingerprint recognition system, and all the records are saved for subsequent operations. Staff biometric attendance system employs an automated system to calculate attendance of staff in an organization and do further calculations of monthly attendance summary in order to reduce human errors during calculations. In essence, the proposed system can be employed in curbing the problems of lateness, buddy punching and truancy in any institution, organization or establishment. The proposed system will also improve the productivity of any organization if properly implemented. Keywords: Institution, Attendance, Biometric, Fingerprin

    Genome sequence of the biocontrol agent coniothyrium minitans conio (IMI 134523)

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    Coniothyrium minitans (synonym, Paraphaeosphaeria minitans) is a highly specific mycoparasite of the wide host range crop pathogen Sclerotinia sclerotiorum. The capability of C. minitans to destroy the sclerotia of S. sclerotiorum has been well recognized and it is available as a widely used biocontrol product Contans WG. We present the draft genome sequence of C. minitans Conio (IMI 134523), which has previously been used in extensive studies that formed part of a registration package of the commercial product. This work provides a distinctive resource for further research into the molecular basis of mycoparasitism to harness the biocontrol potential of C. minitans
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