Família defensiva de peptídeos antimicrobianos em Urochloa.

O capim-braquiária é muito utilizado no Brasil, principalmente no cerrado, como forrageira devido a sua alta adaptabilidade a variados solos, como solos ácidos ou pobres em nutrientes; e apresenta alta cobertura e produção de biomassa. Os peptídeos antimicrobianos são moléculas proteicas pequenas produzidas por todos os seres vivos como parte do sistema imune inato para combater infecções e patógenos. Peptídeos antimicrobianos vegetais possuem um amplo espectro de atividades antimicrobianas contra fitopatógenos e a grande maioria deles é ativa contra fungos, enquanto alguns são inibidores de bactérias e insetos herbívoros. Deste modo, o objetivo deste trabalho foi verificar e identificar a presença de peptídeos antimicrobianos da família das defensinas em U. decumbens por meio da comparação entre as sequências do transcriptoma da planta contra as sequências de aminoácidos de peptídeos antimicrobianos. O transcriptoma da planta foi fornecido pela EMBRAPA Gado de Corte (Campo Grande, MS), obtido pela extração de RNA de plantas expostas a condições de estresse (com alumínio) e sem estresse (sem alumínio). As sequências dos peptídeos antimicrobianos foram baixadas do banco de dados online CAMP e foram formatadas em FASTA em um banco local no computador da FCBA, UFGD. Foi utilizado o blast local para fazer o alinhamento entre os 164.920 transcritos únicos da U. decumbens e as 5.906 sequências de peptídeos antimicrobianos. Deste alinhamento obteve-se 3.161 similaridades com 251 peptídeos diferentes. Para a análise dos resultados utilizou-se o MySQL, separando-os em famílias de acordo com a classificação do banco de dados CAMP e selecionando um grupo amostral para a construção das árvores filogenéticas. A partir desta análise, obteve-se 261 similaridades com 52 peptídeos diferentes dentro da família das defensinas. A maioria dos peptídeos antimicrobianos identificados possui atividade antifúngica ou antimicrobiana, e são de origem vegetal o que condiz com os transcritos da gramínea

Weak formulation for singular diffusion equation with dynamic boundary condition

In this paper, we propose a weak formulation of the singular diffusion equation subject to the dynamic boundary condition. The weak formulation is based on a reformulation method by an evolution equation including the subdifferential of a governing convex energy. Under suitable assumptions, the principal results of this study are stated in forms of Main Theorems A and B, which are respectively to verify: the adequacy of the weak formulation; the common property between the weak solutions and those in regular problems of standard PDEs.Comment: 23 page

Universal computation with limited resources: Belousov-Zhabotinsky and Physarum computers

Using the examples of an excitable chemical system (Belousov-Zhabotinsky medium) and plasmodium of Physarum polycephalum we show that universal computation in a geometrically unconstrained medium is only possible when resources (excitability or concentration of nutrients) are limited. In situations of limited resources the systems studied develop travelling localizations. The localizations are elementary units of dynamical logical circuits in collision-based computing architectures.Comment: Int. J. Bifurcation and Chaos (2008), accepte

Stability of the Magnetopause of Disk-Accreting Rotating Stars

We discuss three modes of oscillation of accretion disks around rotating magnetized neutron stars which may explain the separations of the kilo-Hertz quasi periodic oscillations (QPO) seen in low mass X-ray binaries. The existence of these compressible, non-barotropic magnetohydrodynamic (MHD) modes requires that there be a maximum in the angular velocity $\Omega_\phi(r)$ of the accreting material larger than the angular velocity of the star $\Omega_*$, and that the fluid is in approximately circular motion near this maximum rather than moving rapidly towards the star or out of the disk plane into funnel flows. Our MHD simulations show this type of flow and $\Omega_\phi(r)$ profile. The first mode is a Rossby wave instability (RWI) mode which is radially trapped in the vicinity of the maximum of a key function $g(r){\cal F}(r)$ at $r_{R}$. The real part of the angular frequency of the mode is $\omega_r=m\Omega_\phi(r_{R})$, where $m=1,2...$ is the azimuthal mode number. The second mode, is a mode driven by the rotating, non-axisymmetric component of the star's magnetic field. It has an angular frequency equal to the star's angular rotation rate $\Omega_*$. This mode is strongly excited near the radius of the Lindblad resonance which is slightly outside of $r_R$. The third mode arises naturally from the interaction of flow perturbation with the rotating non-axisymmetric component of the star's magnetic field. It has an angular frequency $\Omega_*/2$. We suggest that the first mode with $m=1$ is associated with the upper QPO frequency, $\nu_u$; that the nonlinear interaction of the first and second modes gives the lower QPO frequency, $\nu_\ell =\nu_u-\nu_*$; and that the nonlinear interaction of the first and third modes gives the lower QPO frequency $\nu_\ell=\nu_u-\nu_*/2$, where $\nu_*=\Omega_*/2\pi$.Comment: 10 pages, 7 figure

Linear Field Dependence of the Normal-State In-Plane Magnetoresistance of Sr2RuO4

The transverse and longitudinal in-plane magnetoresistances in the normal state of superconducting Sr2RuO4 single crystals have been measured. At low temperatures, both of them were found to be positive with a linear magnetic-field dependence above a threshold field, a result not expected from electronic band theory. We argue that such behavior is a manifestation of a novel coherent state characterized by a spin pseudo gap in the quasi-particle excitation spectrum in Sr2RuO4.Comment: 4 pages + 5 figure

Uniaxial-Pressure induced Ferromagnetism of Enhanced Paramagnetic Sr3Ru2O7

We report a uniaxial pressure-dependence of magnetism in layered perovskite strontium ruthenate Sr3Ru2O7. By applying a relatively small uniaxial pressure, greater than 0.1 GPa normal to the RuO2 layer, ferromagnetic ordering manifests below 80 K from the enhanced-paramagnet. Magnetization at 1 kOe and 2 K becomes 100 times larger than that under ambient condition. Uniaxial pressure dependence of Curie temperature T_C suggests the first order magnetic transition. Origin of this uniaxial-pressure induced ferromagnetism is discussed in terms of the rotation of RuO6 octahedra within the RuO2 plane.Comment: 8 pages, 3 figures. to be published in Journal of the Physical Society of Japan, vol.73, No.5 (2004

Millihertz Quasi-periodic Optical Oscillations in 4U 0614+091

We report the discovery of a 1mHz optical quasi-periodic oscillation (QPO) in the candidate ultracompact low-mass X-ray binary 4U 0614+091. The ultra-low frequency QPO has no X-ray counterpart in contemporaneous RXTE/PCA data and is likely a signature of structure in the accretion disk. The QPO can be reasonably fitted with a single sine wave but with a phase jump part way through the observation, indicating that it is not coherent.We also identify a 48 min modulation, approximately consistent with the suggested orbital period of O'Brien (2005) and Shahbaz et al. (2008). If this is indeed orbital, it supports an identification of 4U 0614+091 as an ultra-compact source.Comment: 7 pages, 5 figures, accepted for publication in Monthly Notices of the Royal Astronomical Societ

Discrete Cylindrical Vector Beam Generation from an Array of Optical Fibers

A novel method is presented for the beam shaping of far field intensity distributions of coherently combined fiber arrays. The fibers are arranged uniformly on the perimeter of a circle, and the linearly polarized beams of equal shape are superimposed such that the far field pattern represents an effective radially polarized vector beam, or discrete cylindrical vector (DCV) beam. The DCV beam is produced by three or more beams that each individually have a varying polarization vector. The beams are appropriately distributed in the near field such that the far field intensity distribution has a central null. This result is in contrast to the situation of parallel linearly polarized beams, where the intensity peaks on axis

Insulin regulates arginine-stimulated insulin secretion in humans

Aims: Insulin potentiates glucose-stimulated insulin secretion. These effects are attenuated in beta cell–specific insulin receptor knockout mice and insulin resistant humans. This investigation examines whether short duration insulin exposure regulates beta cell responsiveness to arginine, a non-glucose secretagogue, in healthy humans. Materials and methods: Arginine-stimulated insulin secretion was studied in 10 healthy humans. In each subject arginine was administered as a bolus followed by continuous infusion on two occasions one month apart, after sham/saline or hyperinsulinemic-isoglycemic clamp, respectively providing low and high insulin pre-exposure conditions. Arginine-stimulated insulin secretion was measured by C-peptide deconvolution, and by a selective immunogenic (DAKO) assay for direct measurement of endogenous but not exogenous insulin. Results: Pre-exposure to exogenous insulin augmented arginine-stimulated insulin secretion. The effect was seen acutely following arginine bolus (endogenous DAKO insulin incremental AUC240-255min 311.6 ± 208.1 (post-insulin exposure) versus 120.6 ± 42.2 μU/ml•min (sham/saline) (t-test P = 0.021)), as well as in response to continuous arginine infusion (DAKO insulin incremental AUC260-290min 1095.3 ± 592.1 (sham/saline) versus 564.8 ± 207.1 μU/ml•min (high insulin)(P = 0.009)). Findings were similar when beta cell response was assessed using C-peptide, insulin secretion rates by deconvolution, and the C-peptide to glucose ratio. Conclusions: We demonstrate a physiologic role of insulin in regulation of the beta cell secretory response to arginine