384 research outputs found
Thomas-Ehrman shifts in nuclei around ^{16}O and role of residual nuclear interaction
The asymmetry in the energy spectra between mirror nuclei (the Thomas-Ehrman
shifts) around O is investigated from a phenomenological viewpoint. The
recent data on proton-rich nuclei indicates that the residual nuclear
interaction is reduced for the loosely bound s-orbit by as much as 30%, which
originates in the broad radial distribution of the proton single-particle wave
function.Comment: to appear in Phys. Lett. B, with 3 eps figure
On the density matrix for the kink ground state of higher spin XXZ chain
The exact expression for the density matrix of the kink ground state of
higher spin XXZ chain is obtained
On the problem of calculation of correlation functions in the six-vertex model with domain wall boundary conditions
The problem of calculation of correlation functions in the six-vertex model
with domain wall boundary conditions is addressed by considering a particular
nonlocal correlation function, called row configuration probability. This
correlation function can be used as building block for computing various (both
local and nonlocal) correlation functions in the model. The row configuration
probability is calculated using the quantum inverse scattering method; the
final result is given in terms of a multiple integral. The connection with the
emptiness formation probability, another nonlocal correlation function which
was computed elsewhere using similar methods, is also discussed.Comment: 15 pages, 2 figure
Simultaneous disruption of two DNA polymerases, Polη and Polζ, in Avian DT40 cells unmasks the role of Polη in cellular response to various DNA lesions
Replicative DNA polymerases are frequently stalled by DNA lesions. The resulting replication blockage is released by homologous recombination (HR) and translesion DNA synthesis (TLS). TLS employs specialized TLS polymerases to bypass DNA lesions. We provide striking in vivo evidence of the cooperation between DNA polymerase η, which is mutated in the variant form of the cancer predisposition disorder xeroderma pigmentosum (XP-V), and DNA polymerase ζ by generating POLη−/−/POLζ−/− cells from the chicken DT40 cell line. POLζ−/− cells are hypersensitive to a very wide range of DNA damaging agents, whereas XP-V cells exhibit moderate sensitivity to ultraviolet light (UV) only in the presence of caffeine treatment and exhibit no significant sensitivity to any other damaging agents. It is therefore widely believed that Polη plays a very specific role in cellular tolerance to UV-induced DNA damage. The evidence we present challenges this assumption. The phenotypic analysis of POLη−/−/POLζ−/− cells shows that, unexpectedly, the loss of Polη significantly rescued all mutant phenotypes of POLζ−/− cells and results in the restoration of the DNA damage tolerance by a backup pathway including HR. Taken together, Polη contributes to a much wide range of TLS events than had been predicted by the phenotype of XP-V cells
In Vitro Contraction of Cytokinetic Ring Depends on Myosin II but not on Actin Dynamics
10.1038/ncb2781Nature Cell Biology157853-859NCBI
Anaplastic lymphoma kinase (ALK) inhibitor response in neuroblastoma is highly correlated with ALK mutation status, ALK mRNA and protein levels
Background In pediatric neuroblastoma (NBL), high anaplastic lymphoma kinase (ALK) levels appear to be correlated with an unfavorable prognosis, regardless of ALK mutation status. This suggests a therapeutic role for ALK inhibitors in NBL patients. We examined the correlation between levels of ALK, phosphorylated ALK (pALK) and downstream signaling proteins and response to ALK inhibition in a large panel of both ALK mutated and wild type (WT) NBL cell lines. Methods We measured protein levels by western blot and ALK inhibitor sensitivity (TAE684) by viability assays in 19 NBL cell lines of which 6 had a point mutation and 4 an amplification of the ALK gene. Results ALK 220 kDa (p=0.01) and ALK 140 kDa (p= 0.03) protein levels were higher in ALK mutant than WT cell lines. Response to ALK inhibition was significantly correlated with ALK protein levels (p<0.01). ALK mutant cell lines (n=4) were 14,9 fold (p<0,01) more sensitive to ALK inhibition than eight WT cell lines. Conclusion NBL cell lines often express ALK at high levels and are responsive to ALK inhibitors. Mutated cell lines express ALK at higher levels, which may define their superior response to ALK inhibition
Controlling transmembrane protein concentration and orientation in supported lipid bilayers
The trans-membrane protein – proteorhodopsin (pR) has been incorporated into supported lipid bilayers (SLB). In-plane electric fields have been used to manipulate the orientation and concentration of these proteins, within the SLB, through electrophoresis leading to a 25-fold increase concentration of pR
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