Effects of climate change on pest-parasitoid dynamics: Development of a simulation model and first results

The influence of predicted climatic change on agricultural pest and beneficial insect species is of high importance for growers since increasing temperatures may have a direct impact on the developmental rates of insect populations. These parameters are species-specific and result in variable reactions to climate change, potentially disrupting the synchrony of pest-parasitoid dynamics. This study investigatedthe effect of increasing temperatures caused by climatechange on the population dynamics of the mealy cabbage aphid Brevicoryne brassicae , a worldwide occurring pest species in cruciferous crops, and the endoparasitoid Diaeretiella rapae . For three vegetable growing regions in lower Saxony, the population development of both species was simulatedfor (1) the near future (2041-2050), (2) the far future (2090-2099) and (3) the reference time period (1991-2000), using an age structured simulation model including simulated temperature data. An earlier first occurrence of D. rapae in spring compared to B. brassicae was shown, however with no disruption of the synchrony between the two populations. Despite the earlier occurrence and increased population size of D. rapae in future periods, B. brassicae maximum population size also increased. Furthermore, an earlier occurrence of alate aphids during the season resulted from the model, indicating a potential need to adapt pest management

Native parasitoids and their potential to control the invasive leafminer, Cameraria ohridella DESCH. & DIM. (Lep.: Gracillariidae)

In spite of the fact that since the end of the eighties, the horse chestnut leafminer, Cameraria ohridella, has established itself throughout Europe, native predators such as ants and birds are not attuned to this neozoic species. In contrast, several parasitic wasp species already started to exploit the invasive horse chestnut leafminer, but until now parasitation rates are quite low, mainly because of asynchrony in the lifecycles of parasitoids and host. Only the removal of leaf litter, in which pupae hibernate, is at the moment a strategy to reduce the infestation level in the next year. Unfortunately, not only hibernating horse chestnut leafminers but also parasitoids are removed, and important resources for biocontrol are unused. In the current study, we investigated the potential efficiency of the horse chestnut leafminer parasitoid complex extracted from leaf litter in defined environments. Parasitoids were released at different densities to investigate density dependence in parasitation rates. Although seven different species were released in our experiments, only Pnigalio agraules turned out to be responsible for biocontrol of C. ohridella. We recorded parasitation rates of up to 35%. Overall, parasitation rates were independent of the leafminer density but increased fourfold if ten times more parasitoid individuals were released. Unfortunately, none of the parasitoid species could be established in the experimental units in the long run. Results are compared to other parasitoid-leafminer systems, and promotion of horse chestnut leafminer parasitoids to support natural selection and biological control of the horse chestnut leafminer is discussed. Copyright © 2008 Cambridge University Press

Exceeding the threshold value for Trioza apicalis Förster 1848 in carrot fields did not cause damage as revealed during monitoring in Germany from 2017–2020

The carrot psyllid Trioza apicalis Förster 1848 is a carrot pest in Europe that can cause serious damages in case of massive occurrence. Damages up to a total loss of yield have been reported from Scandinavian countries but also from Switzerland. The action threshold to control the pest with chemical pesticides is 0.2 T. apicalis per day and trap caught by sticky traps. We investigated the number of T. apicalis with sticky traps on carrot fields of the study regions Lüneburg/Uelzen and Hameln/Bad Pyrmont in Germany, during the period 2017–2020. The number of T. apicalis caught was generally very low in both study regions. On several fields in successive weeks almost no individuals were found in the study region Hameln/Bad Pyrmont. In Lüneburg/Uelzen was at least one field each year where the number of carrot psyllid was clearly higher than in all other fields and exceeded the threshold level. Surprisingly on carrot fields in close proximity to carrot fields from the previous year, the T. apicalis numbers were only slightly increased. Nonetheless, no loss of yield was reported for any of the fields in the four years of the study, although the generally defined threshold has been exceeded on many of the investigated carrot fields

Exceeding the threshold value for Trioza apicalis Förster 1848 in carrot fields did not cause damage as revealed during monitoring in Germany from 2017–2020

The carrot psyllid Trioza apicalis Förster 1848 is a carrot pest in Europe that can cause serious damages in case of massive occurrence. Damages up to a total loss of yield have been reported from Scandinavian countries but also from Switzerland. The action threshold to control the pest with chemical pesticides is 0.2 T. apicalis per day and trap caught by sticky traps. We investigated the number of T. apicalis with sticky traps on carrot fields of the study regions Lüneburg/Uelzen and Hameln/Bad Pyrmont in Germany, during the period 2017–2020. The number of T. apicalis caught was generally very low in both study regions. On several fields in successive weeks almost no individuals were found in the study region Hameln/Bad Pyrmont. In Lüneburg/Uelzen was at least one field each year where the number of carrot psyllid was clearly higher than in all other fields and exceeded the threshold level. Surprisingly on carrot fields in close proximity to carrot fields from the previous year, the T. apicalis numbers were only slightly increased. Nonetheless, no loss of yield was reported for any of the fields in the four years of the study, although the generally defined threshold has been exceeded on many of the investigated carrot fields.Bundesanstalt für Landwirtschaft und Ernährung http://dx.doi.org/10.13039/501100010771Bundesanstalt für Landwirtschaft und Ernährung (DE)Julius Kühn-Institut (JKI), Bundesforschungsinstitut für Kulturpflanzen (4250)Peer Reviewe

Ligand-Receptor Interactions

The formation and dissociation of specific noncovalent interactions between a variety of macromolecules play a crucial role in the function of biological systems. During the last few years, three main lines of research led to a dramatic improvement of our understanding of these important phenomena. First, combination of genetic engineering and X ray cristallography made available a simultaneous knowledg of the precise structure and affinity of series or related ligand-receptor systems differing by a few well-defined atoms. Second, improvement of computer power and simulation techniques allowed extended exploration of the interaction of realistic macromolecules. Third, simultaneous development of a variety of techniques based on atomic force microscopy, hydrodynamic flow, biomembrane probes, optical tweezers, magnetic fields or flexible transducers yielded direct experimental information of the behavior of single ligand receptor bonds. At the same time, investigation of well defined cellular models raised the interest of biologists to the kinetic and mechanical properties of cell membrane receptors. The aim of this review is to give a description of these advances that benefitted from a largely multidisciplinar approach

Genomic Organization, Molecular Diversification, and Evolution of Antimicrobial Peptide Myticin-C Genes in the Mussel (Mytilus galloprovincialis)

Myticin-C is a highly variable antimicrobial peptide associated to immune response in Mediterranean mussel (Mytilus galloprovincialis). In this study, we tried to ascertain the genetic organization and the mechanisms underlying myticin-C variation and evolution of this gene family. We took advantage of the large intron size variation to find out the number of myticin-C genes. Using fragment analysis a maximum of four alleles was detected per individual at both introns in a large mussel sample suggesting a minimum of two myticin-C genes. The transmission pattern of size variants in two full-sib families was also used to ascertain the number of myticin-C genes underlying the variability observed. Results in both families were in accordance with two myticin-C genes organized in tandem. A more detailed analysis of myticin-C variation was carried out by sequencing a large sample of complementary (cDNA) and genomic DNA (gDNA) in 10 individuals. Two basic sequences were detected at most individuals and several sequences were constituted by combination of two different basic sequences, strongly suggesting somatic recombination or gene conversion. Slight within-basic sequence variation detected in all individuals was attributed to somatic mutation. Such mutations were more frequently at the C-terminal domain and mostly determined non-synonymous substitutions. The mature peptide domain showed the highest variation both in the whole cDNA and in the basic-sequence samples, which is in accordance with the pathogen recognition function associated to this domain. Although most tests suggested neutrality for myticin-C variation, evidence indicated positive selection in the mature peptide and C-terminal region. Three main highly supported clusters were observed when reconstructing phylogeny on basic sequences, meiotic recombination playing a relevant role on myticin-C evolution. This study demonstrates that mechanisms to generate molecular variation similar to that observed in vertebrates are also operating in molluscs

Effects of lorazepam on saccadic eye movements: the role of sex, task characteristics and baseline traits

Medical Research Council; National Institute for Health Research; Mental Health Biomedical Research Centre at South London; Maudsley NHS Foundation Trust; Institute of Psychiatry, Psychology; Neuroscience, King’s College London

Back on track – On the role of the microtubule for kinesin motility and cellular function

The evolution of cytoskeletal filaments (actin- and intermediate-filaments, and the microtubules) and their associated motor- and non-motor-proteins has enabled the eukaryotic cell to achieve complex organizational and structural tasks. This ability to control cellular transport processes and structures allowed for the development of such complex cellular organelles like cilia or flagella in single-cell organisms and made possible the development and differentiation of multi-cellular organisms with highly specialized, polarized cells. Also, the faithful segregation of large amounts of genetic information during cell division relies crucially on the reorganization and control of the cytoskeleton, making the cytoskeleton a key prerequisite for the development of highly complex genomes. Therefore, it is not surprising that the eukaryotic cell continuously invests considerable resources in the establishment, maintenance, modification and rearrangement of the cytoskeletal filaments and the regulation of its interaction with accessory proteins. Here we review the literature on the interaction between microtubules and motor-proteins of the kinesin-family. Our particular interest is the role of the microtubule in the regulation of kinesin motility and cellular function. After an introduction of the kinesin–microtubule interaction we focus on two interrelated aspects: (1) the active allosteric participation of the microtubule during the interaction with kinesins in general and (2) the possible regulatory role of post-translational modifications of the microtubule in the kinesin–microtubule interaction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42588/1/10974_2005_Article_9052.pd