Shuffle on array languages generated by array grammars

Motivated by the studies done by G. Siromoney et al. (1973) and Alexan- dru Mateescu et al. (1998) we examine the language theoretic results related to shuf- fle on trajectories by making use of Siromoney array grammars such as (R : R)AG, (R : C F )AG, (C F : R)AG, (C F : C F )AG, (C S : R)AG, (C S : C S)AG and (C F : C S)AG which are more powerful than the Siromoney matrix grammars (1972) and are used to make digital pictures

Mass Spectrometry-based Sequencing of Venom Peptides (Conotoxins) from Vermivorous Cone Snail, Conus Loroisii: Toxicity of its Natural Venom

Conus loroisii is a marine vermivorous snail found profusely in the southern seas of India. They harbor several toxic peptide components commonly called as ‘conotoxins’. In this study, we have identified and sequenced five conotoxins using proteome based tandem mass spectrometry analysis through Data analysis 4.1 software. Among them, we found Lo959 as contryphan which is previously described. All other conotoxins Lo1702, Lo1410, Lo1385 and Lo1686 belong to M-Superfamily conotoxins and novel to C. loroisii. Lo1410 is completely novel to conotoxin research with 3 disulfides and the amino acid sequence is derived as CCSTNCAVCIPCCP. All the identified M-Superfamily conotoxins are sub categorised to mini M2 superfamily conotoxins. Lo1702 and Lo1686 possess C- terminal amidation which is the key feature in conotoxins. Moreover, we have screened the natural venom for the occurrence of toxicity in the zebrafish model and brine shrimp

SWAT BASED ASSESSMENT AND PREDICTION OF CLIMATE CHANGE AND ITS IMPACT IN THENPENNAI SUB-BASIN OF SOUTH INDIA

Climate change impacts on watershed ecosystems and hydrologic processes are complex. The key significant parameters responsible for balancing the watershed ecosystems are temperature and rainfall. Though these parameters are uncertain, they play a prime role in the projections of dimensional climate change studies. The impact of climate change is more dependent on temperature and precipitation which contributes at a larger magnitude for characterising global warming issues. This paper aims to forecast the variations of temperature and precipitation during the period of 2020–2050 for the northern part of Thenpennar sub basin. This study is modelled using SWAT (Soil and Water Assessment Tool) – a scale model developed to predict the impact of changes that occurs in land, soil and water over a period of time. This study is validated using the base period from 1980–2000 which shows the distribution of rainfall and temperature among 38 watersheds. The results from this study show that there is a decrease in the rainfall for a maximum of about 20% in the month of December during the predicted period of 2020 and 2050. This study assesses the possible adverse impact of climate change on temperature and precipitation of Thenpennai sub-basin. This kind of predictions will help the government agencies, rulers and decision makers in policy making and implementing the adaptation strategies for the changing climatic conditions

Effect of Humic Acid on Seed Germination of Raphanus sativus L

Abstract: In the present study, we have tested the effect of humic acid on seed germination of Radish (Raphanus sativus). Seeds were soaked in various concentrations (0.1%, 0.25%, 0.5%, 0.75% and 1%) of humic acid at different time periods (10, 60, 120, 180 and 240 minutes). After 7 days, the seeds were analysed for their germination capacity, root and shoot length. The study infers that humic acid with the concentration of 0.25% showed maximum seed germination (100%) and the optimum shoot and root length was recorded as 6.175cm and 11.46cm respectively after 60 minutes soaking

Over-expression of adenosine deaminase in mouse podocytes does not reverse puromycin aminonucleoside resistance

<p>Abstract</p> <p>Background</p> <p>Edema in nephrotic syndrome results from renal retention of sodium and alteration of the permeability properties of capillaries. Nephrotic syndrome induced by puromycin aminonucleoside (PAN) in rats reproduces the biological and clinical signs of the human disease, and has been widely used to identify the cellular mechanisms of sodium retention. Unfortunately, mice do not develop nephrotic syndrome in response to PAN, and we still lack a good mouse model of the disease in which the genetic tools necessary for further characterizing the pathophysiological pathway could be used. Mouse resistance to PAN has been attributed to a defect in glomerular adenosine deaminase (ADA), which metabolizes PAN. We therefore attempted to develop a mouse line sensitive to PAN through induction of normal adenosine metabolism in their podocytes.</p> <p>Methods</p> <p>A mouse line expressing functional ADA under the control of the podocyte-specific podocin promoter was generated by transgenesis. The effect of PAN on urinary excretion of sodium and proteins was compared in rats and in mice over-expressing ADA and in littermates.</p> <p>Results</p> <p>We confirmed that expression of ADA mRNAs was much lower in wild type mouse than in rat glomerulus. Transgenic mice expressed ADA specifically in the glomerulus, and their ADA activity was of the same order of magnitude as in rats. Nonetheless, ADA transgenic mice remained insensitive to PAN treatment in terms of both proteinuria and sodium retention.</p> <p>Conclusions</p> <p>Along with previous results, this study shows that adenosine deaminase is necessary but not sufficient to confer PAN sensitivity to podocytes. ADA transgenic mice could be used as a background strain for further transgenesis.</p

Hepatitis C Virus Protects Human B Lymphocytes from Fas-Mediated Apoptosis via E2-CD81 Engagement

HCV infection is often associated with B-cell regulatory control disturbance and delayed appearance of neutralizing antibodies. CD81 is a cellular receptor for HCV and can bind to HCV envelope protein 2 (E2). CD81 also participates to form a B cell costimulatory complex. To investigate whether HCV influences B cell activation and immune function through E2 -CD81 engagement, here, human Burkitt's lymphoma cell line Raji cells and primary human B lymphocytes (PHB) were treated with HCV E2 protein and cell culture produced HCV particles (HCVcc), and then the related cell phenotypes were assayed. The results showed that both E2 and HCVcc triggered phosphorylation of IκBα, enhanced the expression of anti-apoptosis Bcl-2 family proteins, and protected Raji cells and PHB cells from Fas-mediated death. In addition, both E2 protein and HCVcc increased the expression of costimulatory molecules CD80, CD86 and CD81 itself, and decreased the expression of complement receptor CD21. The effects were dependent on E2-CD81 interaction on the cell surface, since CD81-silenced Raji cells did not respond to both treatments; and an E2 mutant that lose the CD81 binding activity, could not trigger the responses of both Raji cells and PHB cells. The effects were not associated with HCV replication in cells, for HCV pseudoparticle (HCVpp) and HCVcc failed to infect Raji cells. Hence, E2-CD81 engagement may contribute to HCV-associated B cell lymphoproliferative disorders and insufficient neutralizing antibody production

Natural Killer Cell Signal Integration Balances Synapse Symmetry and Migration

Imaging immune surveillance by natural killer (NK) cells has revealed that integration of activating and inhibitory signals determines whether or not NK cells stop to kill the target cell or retain a migratory configuration

Soy isoflavones and their relationship with microflora: beneficial effects on human health in equol producers

The bioavailability of soy isoflavones depends on the composition of the microflora for each subject. Bacteria act on different isoflavones with increased or reduced absorption and cause biotransformation of these compounds into metabolites with higher biological activity. S-equol is the most important metabolite and only 25–65 % of the population have the microflora that produces this compound. The presence of equol-producing bacteria in soy product consumers means that the consumption of such products for prolonged periods leads to lower cardiovascular risk, reduced incidence of prostate and breast cancer, and greater relief from symptoms related to the menopause such as hot flushes and osteoporosis